Restriction map for
pFN21AE1056
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI Asp718I
AspEI AspI AviII AvrII BamHI
BanI BbeI BbrPI BcgI BciVI
BlnI BlpI Bpu1102I BsaBI BsaHI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV FseI FspI
Hin1I KasI KpnI KspI MamI
MluI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PflFI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
StuI Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccIII 778 TCCGGA T^CCGGA
AflII 1921 CTTAAG C^TTAAG
AlwNI 3425 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 406 GGGCCC GGGCC^C
BbsI 1726 GAAGAC GAAGAC(2/6)
BbvCI 3422 CCTCAGC CCTCAGC(-5/-2)
BcnI 3309 CCGGG CC^[GC]GG
BfrI 1921 CTTAAG C^TTAAG
BglI 3316 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3410 AGATCT A^GATCT
BpuAI 1726 GAAGAC GAAGAC(2/6)
BsaI 3304 GGTCTC GGTCTC(1/5)
BsaWI 778 TCCGGA [AT]^CCGG[AT]
BseAI 778 TCCGGA T^CCGGA
BspEI 778 TCCGGA T^CCGGA
BssSI 683 CACGAG CACGAG(-5/-1)
BstEII 995 GGTCACC G^GT[ACGT]ACC
BstPI 995 GGTCACC G^GT[ACGT]ACC
BstXI 3225 CCAATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 501 TTTAAA TTT^AAA
EcoNI 3709 CCTAGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 995 GGTCACC G^GT[ACGT]ACC
EcoT22I 2519 ATGCAT ATGCA^T
FauI 897 CCCGC CCCGC(4/6)
HaeII 3604 AGCGCC [GA]GCGC^[CT]
HgaI 2424 GCGTC GACGC(5/10)
HindIII 770 AAGCTT A^AGCTT
HpaI 196 GTTAAC GTT^AAC
MroI 778 TCCGGA T^CCGGA
NciI 3309 CCGGG CC^[GC]GG
NsiI 2519 ATGCAT ATGCA^T
PshAI 3177 GACTTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 3193 CTGCAG CTGCA^G
SacI 2336 GAGCTC GAGCT^C
SapI 1387 GAAGAGC GCTCTTC(1/4)
ScaI 1575 AGTACT AGT^ACT
SpeI 1027 ACTAGT A^CTAGT
SwaI 500 ATTTAAAT ATTT^AAAT
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AseI 1552 ATTAAT AT^TAAT
2893 ATTAAT
AsnI 1552 ATTAAT AT^TAAT
2893 ATTAAT
BalI 2439 TGGCCA TGG^CCA
2450 TGGCCA
BanII 406 GGGCCC G[GA]GC[CT]^C
2336 GAGCTC
BclI 615 TGATCA T^GATCA
2739 TGATCA
BsaAI 2623 TACGTG [CT]AC^GT[GA]
2743 CACGTA
BsgI 2328 GTGCAG GTGCAG(16/14)
3632 GTGCAG
BsmFI 416 GGGAC GGGAC(10/14)
2553 GGGAC
BspHI 1125 TCATGA T^CATGA
3279 TCATGA
CfoI 2085 GCGC GCG^C
3605 GCGC
DsaI 323 CCATGG C^C[GA][CT]GG
3317 CCATGG
FbaI 615 TGATCA T^GATCA
2739 TGATCA
HapII 779 CCGG C^CGG
3309 CCGG
HhaI 2085 GCGC GCG^C
3605 GCGC
HinP1I 2085 GCGC G^CGC
3605 GCGC
HpaII 779 CCGG C^CGG
3309 CCGG
MluNI 2439 TGGCCA TGG^CCA
2450 TGGCCA
MscI 2439 TGGCCA TGG^CCA
2450 TGGCCA
MspI 779 CCGG C^CGG
3309 CCGG
NcoI 323 CCATGG C^CATGG
3317 CCATGG
NspI 1646 GCATGT [GA]CATG^[CT]
1868 GCATGT
PaeR7I 2072 CTCGAG C^TCGAG
3152 CTCGAG
PpuMI 330 AGGACCT [GA]G^G[AT]CC[CT]
609 GGGTCCT
PshBI 1552 ATTAAT AT^TAAT
2893 ATTAAT
RcaI 1125 TCATGA T^CATGA
3279 TCATGA
XhoI 2072 CTCGAG C^TCGAG
3152 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaI AvaII
BbvI BfaI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstBI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MunI MvaI MwoI
NdeII NlaIII NlaIV NspV PflMI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfuI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoII XmnI