(Circular) (Six-base) MAPSORT of: pFN21AE1056.seq Check: 9258 from: 1 to: 8515 pFN21AE1056 8515 bp With 182 enzymes: SgfI * February 1, 2012 16:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5630 5630 Size: 8515 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6325 278 Size: 53 83 186 936 4789 2468 Fragments arranged by size: 4789 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5858 1937 Size: 3921 4594 Acc65I G'GTAC_C Cuts at: 5836 5836 Size: 8515 AclI AA'CG_TT Cuts at: 1563 6642 7015 1563 Size: 5079 373 3063 Fragments arranged by size: 5079 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2328 2928 3630 5070 6578 7962 8230 1632 Size: 696 600 702 1440 1508 1384 268 1917 Fragments arranged by size: 1917 1508 1440 1384 702 696 600 268 AflII C'TTAA_G Cuts at: 820 1017 3926 820 Size: 197 2909 5409 Fragments arranged by size: 5409 2909 197 AflIII A'CryG_T Cuts at: 1203 7414 8391 1203 Size: 6211 977 1327 Fragments arranged by size: 6211 1327 977 AgeI A'CCGG_T Cuts at: 7346 7346 Size: 8515 AhdI GACnn_n'nnGTC Cuts at: 7244 7244 Size: 8515 AleI CACnn'nnGTG Cuts at: 1166 2609 1166 Size: 1443 7072 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4422 1184 Size: 3238 5277 AlwNI CAG_nnn'CTG Cuts at: 5435 6258 7830 5435 Size: 823 1572 6120 Fragments arranged by size: 6120 1572 823 ApaI G_GGCC'C Cuts at: 2415 2415 Size: 8515 ApaLI G'TGCA_C Cuts at: 6572 7728 6572 Size: 1156 7359 ApoI r'AATT_y Cuts at: 1493 2020 2102 2152 3179 3214 4720 5824 6050 Size: 527 82 50 1027 35 1506 1104 226 Cuts at: 6050 6202 8311 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1506 1104 1027 527 226 152 82 50 35 AseI AT'TA_AT Cuts at: 160 1032 3558 4899 7069 160 Size: 872 2526 1341 2170 1606 Fragments arranged by size: 2526 2170 1606 1341 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8515 AvaI C'yCGr_G Cuts at: 1870 1943 4077 5157 5670 5840 1870 Size: 73 2134 1080 513 170 4545 Fragments arranged by size: 4545 2134 1080 513 170 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2738 2771 2738 Size: 33 8482 BamHI G'GATC_C Cuts at: 5845 6212 5845 Size: 367 8148 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5836 7291 618 Size: 325 217 627 4049 1455 1842 Fragments arranged by size: 4049 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2415 4345 5834 1391 Size: 1024 1930 1489 4072 Fragments arranged by size: 4072 1930 1489 1024 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3738 928 Size: 971 1839 5705 Fragments arranged by size: 5705 1839 971 BbvCI CC'TCA_GC Cuts at: 5428 5428 Size: 8515 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6706 6740 1903 Size: 34 4769 34 3678 Fragments arranged by size: 4769 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6407 7617 6407 Size: 1210 7305 BclI T'GATC_A Cuts at: 1548 2620 4744 1548 Size: 1072 2124 5319 Fragments arranged by size: 5319 2124 1072 BfrBI ATG'CAT Cuts at: 4526 4526 Size: 8515 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5327 7126 136 Size: 107 122 71 631 605 3655 1799 1525 Fragments arranged by size: 3655 1799 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5415 8510 5415 Size: 3095 5420 BlpI GC'TnA_GC Cuts at: 5932 5932 Size: 8515 Bme1580I G_kGCm'C Cuts at: 1790 2415 2734 6576 7732 1790 Size: 625 319 3842 1156 2573 Fragments arranged by size: 3842 2573 1156 625 319 BmgBI CAC'GTC Cuts at: 1318 5483 1318 Size: 4165 4350 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3265 3704 6246 Size: 416 53 83 399 24 1848 439 2542 Cuts at: 6246 7204 442 Size: 958 1753 Fragments arranged by size: 2542 1848 1753 958 439 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8515 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3894 3894 Size: 8515 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3057 3975 5715 7175 1239 Size: 1818 918 1740 1460 2579 Fragments arranged by size: 2579 1818 1740 1460 918 Bpu10I CC'TnA_GC Cuts at: 5428 5428 Size: 8515 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2437 2826 2909 3080 4298 6640 7505 7803 2437 Size: 389 83 171 1218 2342 865 298 3149 Fragments arranged by size: 3149 2342 1218 865 389 298 171 83 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5315 7178 882 Size: 617 3816 1863 2219 Fragments arranged by size: 3816 2219 1863 617 BsaAI yAC'GTr Cuts at: 493 1204 4630 4750 6306 493 Size: 711 3426 120 1556 2702 Fragments arranged by size: 3426 2702 1556 711 120 BsaBI GATnn'nnATC Cuts at: 8509 8509 Size: 8515 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6322 6704 7339 275 Size: 53 83 186 936 4789 382 635 1451 Fragments arranged by size: 4789 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2783 6948 7346 7620 7767 8178 2783 Size: 4165 398 274 147 411 3120 Fragments arranged by size: 4165 3120 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2269 2299 2799 2829 3146 Size: 30 165 30 866 30 500 30 317 Cuts at: 3146 3176 1178 Size: 30 6517 Fragments arranged by size: 6517 866 500 317 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2442 3402 5676 5712 5766 5814 1186 Size: 1256 960 2274 36 54 48 3887 Fragments arranged by size: 3887 2274 1256 960 54 48 36 BseYI C'CCAG_C Cuts at: 2513 2668 4702 7718 8214 2513 Size: 155 2034 3016 496 2814 Fragments arranged by size: 3016 2814 2034 496 155 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4354 5658 1163 Size: 573 2618 1304 4020 Fragments arranged by size: 4020 2618 1304 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5965 6726 7754 1540 Size: 275 186 3964 761 1028 2301 Fragments arranged by size: 3964 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3486 4345 4830 6576 6661 7732 3486 Size: 859 485 1746 85 1071 4269 Fragments arranged by size: 4269 1746 1071 859 485 85 BsmI GAATG_Cn' Cuts at: 3507 3738 4063 6026 6119 3507 Size: 231 325 1963 93 5903 Fragments arranged by size: 5903 1963 325 231 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2415 2734 3486 4345 4830 5834 6576 Size: 399 625 319 752 859 485 1004 742 Cuts at: 6576 6661 7732 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1004 859 752 742 625 485 399 319 85 BspEI T'CCGG_A Cuts at: 2783 8178 2783 Size: 5395 3120 BspHI T'CATG_A Cuts at: 3130 5284 6404 8134 3130 Size: 2154 1120 1730 3511 Fragments arranged by size: 3511 2154 1730 1120 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4673 5365 5630 5870 844 Size: 3829 692 265 240 3489 Fragments arranged by size: 3829 3489 692 265 240 BsrBI CCG'CTC Cuts at: 1394 1823 6402 1394 Size: 429 4579 3507 Fragments arranged by size: 4579 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2188 4394 4650 7010 7184 65 Size: 1183 940 2206 256 2360 174 1396 Fragments arranged by size: 2360 2206 1396 1183 940 256 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7159 7346 1700 Size: 110 5349 187 2869 Fragments arranged by size: 5349 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8515 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8515 BssSI C'ACGA_G Cuts at: 2688 6575 7587 2688 Size: 3887 1012 3616 Fragments arranged by size: 3887 3616 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2860 4532 5248 794 Size: 2066 1672 716 4061 Fragments arranged by size: 4061 2066 1672 716 BstBI TT'CG_AA Cuts at: 2215 2958 5152 7319 2215 Size: 743 2194 2167 3411 Fragments arranged by size: 3411 2194 2167 743 BstEII G'GTnAC_C Cuts at: 1184 3000 1184 Size: 1816 6699 BstXI CCAn_nnnn'nTGG Cuts at: 5237 5237 Size: 8515 BstYI r'GATC_y Cuts at: 1972 2583 2911 5415 5732 5845 6212 6597 6614 Size: 611 328 2504 317 113 367 385 17 Cuts at: 6614 8066 8152 8164 8510 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2504 1977 1452 611 385 367 346 328 317 113 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2328 5322 513 Size: 552 939 324 2994 3706 Fragments arranged by size: 3706 2994 939 552 324 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7776 Fragments arranged by size: 7776 683 56 BtsI GCAGTG_nn' Cuts at: 2085 4221 5581 6031 6825 6845 2085 Size: 2136 1360 450 794 20 3755 Fragments arranged by size: 3755 2136 1360 794 450 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8515 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4129 4164 529 Size: 35 3565 35 4880 Fragments arranged by size: 4880 3565 35 35 DraI TTT'AAA Cuts at: 2508 5819 2508 Size: 3311 5204 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7522 809 Size: 507 6206 1802 Fragments arranged by size: 6206 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4300 4444 4455 Size: 54 1425 50 275 18 2470 144 11 Cuts at: 4455 5962 6851 8480 8 Size: 1507 889 1629 43 Fragments arranged by size: 2470 1629 1507 1425 889 275 144 54 50 43 18 11 EagI C'GGCC_G Cuts at: 1537 1812 5962 1537 Size: 275 4150 4090 Fragments arranged by size: 4150 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2346 3387 4223 5791 6445 1353 Size: 993 1041 836 1568 654 3423 Fragments arranged by size: 3423 1568 1041 993 836 654 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2256 7102 7476 7622 387 Size: 1064 138 667 4846 374 146 1280 Fragments arranged by size: 4846 1280 1064 667 374 146 138 EcoICRI GAG'CTC Cuts at: 4343 4343 Size: 8515 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2328 2928 3057 3630 3975 5070 5715 Size: 393 696 600 129 573 345 1095 645 Cuts at: 5715 6578 7175 7962 8230 1239 Size: 863 597 787 268 1524 Fragments arranged by size: 1524 1095 863 787 696 645 600 597 573 393 345 268 129 EcoNI CCTnn'n_nnAGG Cuts at: 5718 5718 Size: 8515 EcoO109I rG'GnC_Cy Cuts at: 2336 2388 2615 3053 5711 5766 2336 Size: 52 227 438 2658 55 5085 Fragments arranged by size: 5085 2658 438 227 55 52 EcoRI G'AATT_C Cuts at: 5824 6202 5824 Size: 378 8137 FspI TGC'GCA Cuts at: 7021 7021 Size: 8515 HaeII r_GCGC'y Cuts at: 1678 5613 7662 1678 Size: 3935 2049 2531 Fragments arranged by size: 3935 2531 2049 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2435 2467 4626 4658 5140 Size: 32 135 32 863 32 2159 32 482 Cuts at: 5140 5172 7156 7188 7230 7262 1373 Size: 32 1984 32 42 32 2626 Fragments arranged by size: 2626 2159 1984 863 482 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2203 4548 4929 5859 6105 669 Size: 988 281 265 2345 381 930 246 3079 Fragments arranged by size: 3079 2345 988 930 381 281 265 246 HindIII A'AGCT_T Cuts at: 748 2775 748 Size: 2027 6488 HpaI GTT'AAC Cuts at: 2203 6105 2203 Size: 3902 4613 KpnI G_GTAC'C Cuts at: 5840 5840 Size: 8515 MfeI C'AATT_G Cuts at: 3673 3881 4464 6114 3673 Size: 208 583 1650 6074 Fragments arranged by size: 6074 1650 583 208 MluI A'CGCG_T Cuts at: 8391 8391 Size: 8515 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3039 3099 4096 5355 7629 7813 1305 Size: 1734 60 997 1259 2274 184 2007 Fragments arranged by size: 2274 2007 1734 1259 997 184 60 MscI TGG'CCA Cuts at: 10 64 1489 1832 4446 4457 10 Size: 54 1425 343 2614 11 4068 Fragments arranged by size: 4068 2614 1425 343 54 11 MslI CAynn'nnrTG Cuts at: 518 1166 2144 2609 3743 6473 6832 6991 518 Size: 648 978 465 1134 2730 359 159 2042 Fragments arranged by size: 2730 2042 1134 978 648 465 359 159 MspA1I CmG'CkG Cuts at: 1606 2668 2860 5432 5931 6608 7756 8001 1606 Size: 1062 192 2572 499 677 1148 245 2120 Fragments arranged by size: 2572 2120 1148 1062 677 499 245 192 NcoI C'CATG_G Cuts at: 513 1065 2004 2328 5322 513 Size: 552 939 324 2994 3706 Fragments arranged by size: 3706 2994 939 552 324 NdeI CA'TA_TG Cuts at: 387 387 Size: 8515 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8515 NotI GC'GGCC_GC Cuts at: 5962 5962 Size: 8515 NsiI A_TGCA'T Cuts at: 4528 4528 Size: 8515 NspI r_CATG'y Cuts at: 1132 1229 3655 3877 5873 7418 1132 Size: 97 2426 222 1996 1545 2229 Fragments arranged by size: 2426 2229 1996 1545 222 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8515 PciI A'CATG_T Cuts at: 7414 7414 Size: 8515 PflMI CCAn_nnn'nTGG Cuts at: 1108 2252 4031 4453 1108 Size: 1144 1779 422 5170 Fragments arranged by size: 5170 1779 1144 422 PfoI T'CCnGG_A Cuts at: 3952 3952 Size: 8515 PmeI GTTT'AAAC Cuts at: 5819 5819 Size: 8515 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6584 6616 8097 8129 6584 Size: 32 1481 32 6970 Fragments arranged by size: 6970 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2336 2615 2336 Size: 279 8236 PshAI GACnn'nnGTC Cuts at: 1613 5186 1613 Size: 3573 4942 PsiI TTA'TAA Cuts at: 2133 6085 2133 Size: 3952 4563 PspOMI G'GGCC_C Cuts at: 2411 2411 Size: 8515 PspXI vC'TCGA_Gb Cuts at: 5157 5157 Size: 8515 PstI C_TGCA'G Cuts at: 830 5202 5867 830 Size: 4372 665 3478 Fragments arranged by size: 4372 3478 665 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8515 PvuII CAG'CTG Cuts at: 2668 2860 5432 2668 Size: 192 2572 5751 Fragments arranged by size: 5751 2572 192 SacI G_AGCT'C Cuts at: 4345 4345 Size: 8515 SalI G'TCGA_C Cuts at: 1936 5857 1936 Size: 3921 4594 SapI GCTCTTCn'nnn_ Cuts at: 3387 3387 Size: 8515 SbfI CC_TGCA'GG Cuts at: 5867 5867 Size: 8515 ScaI AGT'ACT Cuts at: 3582 6763 8475 3582 Size: 3181 1712 3622 Fragments arranged by size: 3622 3181 1712 SfcI C'TryA_G Cuts at: 826 1045 2295 3415 3474 5198 5569 5656 5704 Size: 219 1250 1120 59 1724 371 87 48 Cuts at: 5704 5725 5863 6998 7679 7870 826 Size: 21 138 1135 681 191 1471 Fragments arranged by size: 1724 1471 1250 1135 1120 681 371 219 191 138 87 59 48 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8515 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8515 SmaI CCC'GGG Cuts at: 1872 5842 1872 Size: 3970 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2452 2841 2888 3095 3926 4077 Size: 197 926 509 389 47 207 831 151 Cuts at: 4077 4277 5157 6619 7520 7782 820 Size: 200 880 1462 901 262 1553 Fragments arranged by size: 1553 1462 926 901 880 831 509 389 262 207 200 197 151 47 SnaBI TAC'GTA Cuts at: 493 6306 493 Size: 5813 2702 SpeI A'CTAG_T Cuts at: 152 3032 152 Size: 2880 5635 SphI G_CATG'C Cuts at: 1132 5873 1132 Size: 4741 3774 SspI AAT'ATT Cuts at: 5 52 2567 3665 3722 4147 6439 5 Size: 47 2515 1098 57 425 2292 2081 Fragments arranged by size: 2515 2292 2081 1098 425 57 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8515 StyI C'CwwG_G Cuts at: 513 1065 2004 2328 2535 2587 2996 3026 4125 Size: 552 939 324 207 52 409 30 1099 Cuts at: 4125 5322 5954 513 Size: 1197 632 3074 Fragments arranged by size: 3074 1197 1099 939 632 552 409 324 207 52 30 SwaI ATTT'AAAT Cuts at: 2508 2508 Size: 8515 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 2439 2621 2975 3318 4784 6542 Size: 1255 417 442 182 354 343 1466 1758 Cuts at: 6542 6559 6712 6897 7342 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1758 1498 1466 1255 445 442 417 354 343 185 182 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2601 3470 3580 Size: 274 80 33 51 1443 624 869 110 Cuts at: 3580 3930 5459 6761 8473 96 Size: 350 1529 1302 1712 138 Fragments arranged by size: 1712 1529 1443 1302 869 624 350 274 138 110 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2799 2831 3250 3282 8475 8507 2799 Size: 32 419 32 5193 32 2807 Fragments arranged by size: 5193 2807 419 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8515 XbaI T'CTAG_A Cuts at: 2485 3280 5420 5851 2485 Size: 795 2140 431 5149 Fragments arranged by size: 5149 2140 795 431 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3015 5329 5753 3015 Size: 2314 424 5777 Fragments arranged by size: 5777 2314 424 XhoI C'TCGA_G Cuts at: 1943 4077 5157 1943 Size: 2134 1080 5301 Fragments arranged by size: 5301 2134 1080 XmaI C'CCGG_G Cuts at: 1870 5840 1870 Size: 3970 4545 XmnI GAAnn'nnTTC Cuts at: 2241 3723 4217 5148 6644 2241 Size: 1482 494 931 1496 4112 Fragments arranged by size: 4112 1496 1482 931 494 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6323 276 Size: 53 83 186 936 4789 2468 Fragments arranged by size: 4789 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI