################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmI BsoBI BspDI BspEI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV FbaI FseI HaeII HpaI KasI KpnI Ksp632I MamI MluI MluNI MroI MscI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 75 enzymes) # ################################### AccII 792 CGCG CG^CG AcyI 4 GACGCC G[GA]^CG[CT]C AlwI 406 GATCC GGATC(4/5) AlwNI 1063 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 1054 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 454 TGCGCA TGC^GCA BanII 880 GAGCCC G[GA]GC[CT]^C BbsI 1054 GAAGAC GAAGAC(2/6) BbvI 48 GCTGC GCAGC(8/12) BcnI 1023 CCGGG CC^[GC]GG BfaI 190 CTAG C^TAG BlpI 807 GCTTAGC GC^T[ACGT]AGC BpmI 763 CTGGAG CTGGAG(16/14) Bpu1102I 807 GCTTAGC GC^T[ACGT]AGC BpuAI 1054 GAAGAC GAAGAC(2/6) BsaAI 442 TACGTG [CT]AC^GT[GA] BsaHI 4 GACGCC G[GA]^CG[CT]C BsgI 694 GTGCAG GTGCAG(16/14) BsmAI 463 GAGAC GTCTC(1/5) BsmBI 463 GAGACG CGTCTC(1/5) Bsp1407I 716 TGTACA T^GTACA BspHI 1079 TCATGA T^CATGA BspLU11I 527 ACATGT A^CATGT BsrDI 923 GCAATG GCAATG(2/0) BsrGI 716 TGTACA T^GTACA BstEII 618 GGTCACC G^GT[ACGT]ACC BstPI 618 GGTCACC G^GT[ACGT]ACC BstUI 792 CGCG CG^CG BstYI 405 AGATCC [GA]^GATC[CT] CelII 807 GCTTAGC GC^T[ACGT]AGC CfoI 455 GCGC GCG^C DpnII 406 GATC ^GATC DraIII 55 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 618 GGTCACC G^GT[ACGT]ACC EcoT14I 797 CCATGG C^C[AT][AT]GG EcoT22I 1099 ATGCAT ATGCA^T FspI 454 TGCGCA TGC^GCA HapII 1023 CCGG C^CGG HgaI 4 GACGC GACGC(5/10) HhaI 455 GCGC GCG^C Hin1I 4 GACGCC G[GA]^CG[CT]C HinP1I 455 GCGC G^CGC HincII 22 GTCAAC GT[CT]^[GA]AC HindII 22 GTCAAC GT[CT]^[GA]AC HindIII 805 AAGCTT A^AGCTT HpaII 1023 CCGG C^CGG KspI 791 CCGCGG CCGC^GG MaeI 190 CTAG C^TAG MboI 406 GATC ^GATC MfeI 1049 CAATTG C^AATTG MflI 405 AGATCC [GA]^GATC[CT] MslI 621 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 791 CCGCGG C[AC]G^C[GT]G MspI 1023 CCGG C^CGG MunI 1049 CAATTG C^AATTG MvnI 792 CGCG CG^CG NciI 1023 CCGGG CC^[GC]GG NcoI 797 CCATGG C^CATGG NdeII 406 GATC ^GATC NheI 189 GCTAGC G^CTAGC NsiI 1099 ATGCAT ATGCA^T NspI 527 ACATGT [GA]CATG^[CT] PleI 126 GAGTC GAGTC(4/5) RcaI 1079 TCATGA T^CATGA SacII 791 CCGCGG CCGC^GG Sau3AI 406 GATC ^GATC ScaI 513 AGTACT AGT^ACT SmlI 854 CTCAAG C^T[CT][GA]AG SspBI 716 TGTACA T^GTACA SspI 904 AATATT AAT^ATT StyI 797 CCATGG C^C[AT][AT]GG TseI 48 GCTGC G^C[AT]GC XcmI 594 CCAGAAGAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 405 AGATCC [GA]^GATC[CT] XmnI 1054 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AcsI 398 AAATTC [GA]^AATT[CT] 1087 AAATTT AfaI 514 GTAC GT^AC 717 GTAC AflIII 443 ACGTGT A^C[GA][CT]GT 527 ACATGT ApoI 398 AAATTC [GA]^AATT[CT] 1087 AAATTT AvaII 37 GGTCC G^G[AT]CC 678 GGTCC BanI 589 GGTGCC G^G[CT][GA]CC 787 GGTGCC BspMI 644 ACCTGC ACCTGC(4/8) 15 GCAGGT BstF5I 1020 CATCC GGATG(2/0) 558 GGATG FauI 40 CCCGC CCCGC(4/6) 180 GCGGG FokI 1020 CATCC GGATG(9/13) 558 GGATG RsaI 514 GTAC GT^AC 717 GTAC TfiI 171 GAATC G^A[AT]TC 860 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AluI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI BstXI Cac8I Cfr13I DdeI DsaI EaeI EcoRII Fnu4HI HaeIII HinfI HphI ItaI MaeII MaeIII MboII MnlI MseI MvaI MwoI NlaIII NlaIV Sau96I ScrFI SfaNI Tru9I Tsp45I Tsp509I TspRI