(Circular) (Six-base) MAPSORT of: pFN21AB6181.seq Check: 8138 from: 1 to: 6682 pFN21AB6181 6682 bp With 182 enzymes: SgfI * July 29, 2009 14:18 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3493 4492 278 Size: 53 83 186 936 1957 999 2468 Fragments arranged by size: 2468 1957 999 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3813 4025 1937 Size: 1876 212 4594 Fragments arranged by size: 4594 1876 212 Acc65I G'GTAC_C Cuts at: 4003 4003 Size: 6682 AclI AA'CG_TT Cuts at: 1563 4809 5182 1563 Size: 3246 373 3063 Fragments arranged by size: 3246 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 1999 3218 3650 4745 6129 6397 1632 Size: 367 1219 432 1095 1384 268 1917 Fragments arranged by size: 1917 1384 1219 1095 432 367 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6485 AflIII A'CryG_T Cuts at: 1203 5581 6558 1203 Size: 4378 977 1327 Fragments arranged by size: 4378 1327 977 AgeI A'CCGG_T Cuts at: 5513 5513 Size: 6682 AhdI GACnn_n'nnGTC Cuts at: 5411 5411 Size: 6682 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6682 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6682 AlwNI CAG_nnn'CTG Cuts at: 2215 4425 5997 2215 Size: 2210 1572 2900 Fragments arranged by size: 2900 2210 1572 ApaI G_GGCC'C Cuts at: 2693 2693 Size: 6682 ApaLI G'TGCA_C Cuts at: 4739 5895 4739 Size: 1156 5526 ApoI r'AATT_y Cuts at: 1493 2588 3612 3941 3991 4217 4369 6478 1493 Size: 1095 1024 329 50 226 152 2109 1697 Fragments arranged by size: 2109 1697 1095 1024 329 226 152 50 AseI AT'TA_AT Cuts at: 160 1032 3185 5236 160 Size: 872 2153 2051 1606 Fragments arranged by size: 2153 2051 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6682 AvaI C'yCGr_G Cuts at: 1870 1943 4007 1870 Size: 73 2064 4545 Fragments arranged by size: 4545 2064 73 BamHI G'GATC_C Cuts at: 4012 4379 4012 Size: 367 6315 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2054 2091 4003 5458 618 Size: 325 217 627 267 37 1912 1455 1842 Fragments arranged by size: 1912 1842 1455 627 325 267 217 37 BanII G_rGCy'C Cuts at: 1391 2686 2693 4001 1391 Size: 1295 7 1308 4072 Fragments arranged by size: 4072 1308 1295 7 BbeI G_GCGC'C Cuts at: 2095 2095 Size: 6682 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2755 2881 928 Size: 971 856 126 4729 Fragments arranged by size: 4729 971 856 126 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4873 4907 1903 Size: 34 2936 34 3678 Fragments arranged by size: 3678 2936 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4574 5784 4574 Size: 1210 5472 BclI T'GATC_A Cuts at: 1548 3331 1548 Size: 1783 4899 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5293 136 Size: 107 122 71 631 605 337 3284 1525 Fragments arranged by size: 3284 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6677 6677 Size: 6682 BlpI GC'TnA_GC Cuts at: 4099 4099 Size: 6682 Bme1580I G_kGCm'C Cuts at: 1790 2693 4743 5899 1790 Size: 903 2050 1156 2573 Fragments arranged by size: 2573 2050 1156 903 BmgBI CAC'GTC Cuts at: 1318 3817 1318 Size: 2499 4183 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3766 4413 5371 Size: 416 53 83 399 24 2349 647 958 Cuts at: 5371 442 Size: 1753 Fragments arranged by size: 2349 1753 958 647 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6682 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2492 3994 2492 Size: 1502 5180 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3315 5342 1239 Size: 2076 2027 2579 Fragments arranged by size: 2579 2076 2027 Bpu10I CC'TnA_GC Cuts at: 2685 2685 Size: 6682 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2182 3026 3093 4807 5672 5970 2182 Size: 844 67 1714 865 298 2894 Fragments arranged by size: 2894 1714 865 844 298 67 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3241 3369 3529 5345 882 Size: 617 1742 128 160 1816 2219 Fragments arranged by size: 2219 1816 1742 617 160 128 BsaAI yAC'GTr Cuts at: 493 1204 4473 493 Size: 711 3269 2702 Fragments arranged by size: 3269 2702 711 BsaBI GATnn'nnATC Cuts at: 3207 6676 3207 Size: 3469 3213 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2092 3490 4489 4871 Size: 53 83 186 936 559 1398 999 382 Cuts at: 4871 5506 275 Size: 635 1451 Fragments arranged by size: 1451 1398 999 936 635 559 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5115 5513 5787 5934 6345 5115 Size: 398 274 147 411 5452 Fragments arranged by size: 5452 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3255 3285 1178 Size: 30 165 30 1852 30 4575 Fragments arranged by size: 4575 1852 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2032 2510 1186 Size: 846 478 5358 Fragments arranged by size: 5358 846 478 BseYI C'CCAG_C Cuts at: 2692 3134 3684 5885 6381 2692 Size: 442 550 2201 496 2993 Fragments arranged by size: 2993 2201 550 496 442 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2093 3822 1163 Size: 573 357 1729 4023 Fragments arranged by size: 4023 1729 573 357 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4132 4893 5921 1540 Size: 275 186 2131 761 1028 2301 Fragments arranged by size: 2301 2131 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2686 4743 4828 5899 2686 Size: 2057 85 1071 3469 Fragments arranged by size: 3469 2057 1071 85 BsmI GAATG_Cn' Cuts at: 3096 3797 4193 4286 3096 Size: 701 396 93 5492 Fragments arranged by size: 5492 701 396 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2686 2693 4001 4743 4828 5899 1391 Size: 399 896 7 1308 742 85 1071 2174 Fragments arranged by size: 2174 1308 1071 896 742 399 85 7 BspEI T'CCGG_A Cuts at: 6345 6345 Size: 6682 BspHI T'CATG_A Cuts at: 3901 4571 6301 3901 Size: 670 1730 4282 Fragments arranged by size: 4282 1730 670 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3921 4037 844 Size: 3077 116 3489 Fragments arranged by size: 3489 3077 116 BsrBI CCG'CTC Cuts at: 1394 1823 4569 1394 Size: 429 2746 3507 Fragments arranged by size: 3507 2746 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5177 5351 65 Size: 1183 3929 174 1396 Fragments arranged by size: 3929 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3158 5326 5513 1700 Size: 110 1348 2168 187 2869 Fragments arranged by size: 2869 2168 1348 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6682 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6682 BssSI C'ACGA_G Cuts at: 4742 5754 4742 Size: 1012 5670 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6682 BstBI TT'CG_AA Cuts at: 2746 5486 2746 Size: 2740 3942 BstEII G'GTnAC_C Cuts at: 1184 2990 1184 Size: 1806 4876 BstYI r'GATC_y Cuts at: 1972 4012 4379 4764 4781 6233 6319 6331 6677 Size: 2040 367 385 17 1452 86 12 346 Cuts at: 6677 1972 Size: 1977 Fragments arranged by size: 2040 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5191 Fragments arranged by size: 5191 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2377 3758 487 Size: 683 56 1151 1381 3411 Fragments arranged by size: 3411 1381 1151 683 56 BtsI GCAGTG_nn' Cuts at: 4198 4992 5012 4198 Size: 794 20 5868 Fragments arranged by size: 5868 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6682 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2968 3003 529 Size: 35 2404 35 4208 Fragments arranged by size: 4208 2404 35 35 DraI TTT'AAA Cuts at: 3207 3986 3207 Size: 779 5903 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5689 809 Size: 507 4373 1802 Fragments arranged by size: 4373 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3156 4129 5018 Size: 54 1425 50 275 18 1326 973 889 Cuts at: 5018 6647 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1326 973 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4129 1537 Size: 275 2317 4090 Fragments arranged by size: 4090 2317 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3869 4612 1353 Size: 2516 743 3423 Fragments arranged by size: 3423 2516 743 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5269 5643 5789 387 Size: 1064 138 3680 374 146 1280 Fragments arranged by size: 3680 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2684 2684 Size: 6682 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1999 3218 3315 3650 4745 5342 6129 Size: 393 367 1219 97 335 1095 597 787 Cuts at: 6129 6397 1239 Size: 268 1524 Fragments arranged by size: 1524 1219 1095 787 597 393 367 335 268 97 EcoNI CCTnn'n_nnAGG Cuts at: 2492 3507 2492 Size: 1015 5667 EcoO109I rG'GnC_Cy Cuts at: 2689 2689 Size: 6682 EcoRI G'AATT_C Cuts at: 3941 3991 4369 3941 Size: 50 378 6254 Fragments arranged by size: 6254 378 50 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2395 3655 2395 Size: 1260 5422 FspI TGC'GCA Cuts at: 5188 5188 Size: 6682 HaeII r_GCGC'y Cuts at: 1678 2095 5829 1678 Size: 417 3734 2531 Fragments arranged by size: 3734 2531 417 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2367 2399 3255 3287 5323 Size: 32 135 32 795 32 856 32 2036 Cuts at: 5323 5355 5397 5429 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2036 856 795 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2667 3814 4026 4272 669 Size: 988 281 729 1147 212 246 3079 Fragments arranged by size: 3079 1147 988 729 281 246 212 HindIII A'AGCT_T Cuts at: 748 2508 748 Size: 1760 4922 HpaI GTT'AAC Cuts at: 2667 4272 2667 Size: 1605 5077 KasI G'GCGC_C Cuts at: 2091 2091 Size: 6682 KpnI G_GTAC'C Cuts at: 4007 4007 Size: 6682 MfeI C'AATT_G Cuts at: 2676 4281 2676 Size: 1605 5077 MluI A'CGCG_T Cuts at: 6558 6558 Size: 6682 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5796 5980 1305 Size: 4491 184 2007 Fragments arranged by size: 4491 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4860 Fragments arranged by size: 4860 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3921 4640 4999 5158 518 Size: 648 2755 719 359 159 2042 Fragments arranged by size: 2755 2042 719 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2566 4098 4775 5923 6168 1606 Size: 960 1532 677 1148 245 2120 Fragments arranged by size: 2120 1532 1148 960 677 245 NarI GG'CG_CC Cuts at: 2092 2092 Size: 6682 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5191 Fragments arranged by size: 5191 939 552 NdeI CA'TA_TG Cuts at: 387 3918 387 Size: 3531 3151 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6682 NotI GC'GGCC_GC Cuts at: 4129 4129 Size: 6682 NspI r_CATG'y Cuts at: 1132 1229 4040 5585 1132 Size: 97 2811 1545 2229 Fragments arranged by size: 2811 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6682 PciI A'CATG_T Cuts at: 5581 5581 Size: 6682 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6682 PmeI GTTT'AAAC Cuts at: 3986 3986 Size: 6682 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4751 4783 6264 6296 4751 Size: 32 1481 32 5137 Fragments arranged by size: 5137 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6682 PsiI TTA'TAA Cuts at: 2523 3323 4252 2523 Size: 800 929 4953 Fragments arranged by size: 4953 929 800 PspOMI G'GGCC_C Cuts at: 2689 2689 Size: 6682 PstI C_TGCA'G Cuts at: 830 4034 830 Size: 3204 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6682 PvuII CAG'CTG Cuts at: 2566 2566 Size: 6682 SacI G_AGCT'C Cuts at: 2686 2686 Size: 6682 SalI G'TCGA_C Cuts at: 1936 3812 4024 1936 Size: 1876 212 4594 Fragments arranged by size: 4594 1876 212 SbfI CC_TGCA'GG Cuts at: 4034 4034 Size: 6682 ScaI AGT'ACT Cuts at: 2024 3195 3627 3908 4930 6642 2024 Size: 1171 432 281 1022 1712 2064 Fragments arranged by size: 2064 1712 1171 1022 432 281 SexAI A'CCwGG_T Cuts at: 3286 3286 Size: 6682 SfcI C'TryA_G Cuts at: 826 1045 4030 5165 5846 6037 826 Size: 219 2985 1135 681 191 1471 Fragments arranged by size: 2985 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2093 2093 Size: 6682 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6682 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6682 SmaI CCC'GGG Cuts at: 1872 4009 1872 Size: 2137 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2197 3041 3108 4786 5687 5949 Size: 197 926 254 844 67 1678 901 262 Cuts at: 5949 820 Size: 1553 Fragments arranged by size: 1678 1553 926 901 844 262 254 197 67 SnaBI TAC'GTA Cuts at: 493 4473 493 Size: 3980 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6682 SphI G_CATG'C Cuts at: 1132 4040 1132 Size: 2908 3774 SspI AAT'ATT Cuts at: 5 52 4606 5 Size: 47 4554 2081 Fragments arranged by size: 4554 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6682 StyI C'CwwG_G Cuts at: 513 1065 2004 3550 4121 513 Size: 552 939 1546 571 3074 Fragments arranged by size: 3074 1546 939 571 552 SwaI ATTT'AAAT Cuts at: 3207 3207 Size: 6682 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4709 4726 4879 5064 5509 325 Size: 1255 3129 17 153 185 445 1498 Fragments arranged by size: 3129 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2022 3047 3193 Size: 274 80 33 51 1443 45 1025 146 Cuts at: 3193 3265 3625 3906 4928 6640 96 Size: 72 360 281 1022 1712 138 Fragments arranged by size: 1712 1443 1025 1022 360 281 274 146 138 80 72 51 45 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6642 6674 6642 Size: 32 6650 Tth111I GACn'n_nGTC Cuts at: 1316 3647 1316 Size: 2331 4351 XbaI T'CTAG_A Cuts at: 3064 4018 3064 Size: 954 5728 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6682 XmaI C'CCGG_G Cuts at: 1870 4007 1870 Size: 2137 4545 XmnI GAAnn'nnTTC Cuts at: 2366 3442 4811 2366 Size: 1076 1369 4237 Fragments arranged by size: 4237 1369 1076 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3491 4490 276 Size: 53 83 186 936 1957 999 2468 Fragments arranged by size: 2468 1957 999 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI XcmI