################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII AccI AccIII AciI AclI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AspEI AspI AvaI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbvCI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KspI MaeII MamI MfeI MflI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 255 GGTACC G^GTACC AccII 76 CGCG CG^CG Alw44I 1009 GTGCAC G^TGCAC AlwI 487 GATCC GGATC(4/5) ApaLI 1009 GTGCAC G^TGCAC AseI 31 ATTAAT AT^TAAT AsnI 31 ATTAAT AT^TAAT Asp700I 1120 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 255 GGTACC G^GTACC AspHI 1009 GTGCAC G[AT]GC[AT]^C BalI 965 TGGCCA TGG^CCA BbsI 418 GAAGAC GAAGAC(2/6) BcgI 909 GCACTTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 109 CCCGG CC^[GC]GG BlpI 1049 GCTTAGC GC^T[ACGT]AGC BpmI 788 CTCCAG CTGGAG(16/14) Bpu1102I 1049 GCTTAGC GC^T[ACGT]AGC BpuAI 418 GAAGAC GAAGAC(2/6) BsaI 661 GAGACC GGTCTC(1/5) BsaJI 1280 CCAAGG C^C[ACGT][ACGT]GG BsgI 1259 CTGCAC GTGCAG(16/14) BsiHKAI 1009 GTGCAC G[AT]GC[AT]^C BsmFI 137 GGGAC GGGAC(10/14) Bsp1407I 668 TGTACA T^GTACA BsrGI 668 TGTACA T^GTACA BstUI 76 CGCG CG^CG BstXI 1280 CCAAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1049 GCTTAGC GC^T[ACGT]AGC Cfr13I 87 GGCCC G^G[ACGT]CC DraI 876 TTTAAA TTT^AAA EaeI 965 TGGCCA [CT]^GGCC[GA] EcoNI 423 CCTGTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1254 GAATTC G^AATTC EcoT14I 1280 CCAAGG C^C[AT][AT]GG HaeII 3 GGCGCT [GA]GCGC^[CT] HapII 110 CCGG C^CGG HpaII 110 CCGG C^CGG KpnI 255 GGTACC GGTAC^C MluNI 965 TGGCCA TGG^CCA MscI 965 TGGCCA TGG^CCA MspI 110 CCGG C^CGG MvnI 76 CGCG CG^CG NciI 109 CCCGG CC^[GC]GG NspI 1224 GCATGC [GA]CATG^[CT] PflMI 790 CCAGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 11 GAGTC GAGTC(4/5) PshAI 421 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 31 ATTAAT AT^TAAT SapI 547 GAAGAGC GCTCTTC(1/4) Sau96I 87 GGCCC G^G[ACGT]CC SmlI 541 CTTGAG C^T[CT][GA]AG SpeI 1263 ACTAGT A^CTAGT SphI 1224 GCATGC GCATG^C SspBI 668 TGTACA T^GTACA StyI 1280 CCAAGG C^C[AT][AT]GG Van91I 790 CCAGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 968 CCAAATACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1120 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BanI 255 GGTACC G^G[CT][GA]CC 267 GGTGCC BfaI 625 CTAG C^TAG 1264 CTAG BstNI 17 CCAGG CC^[AT]GG 1146 CCTGG EarI 1124 CTCTTC CTCTTC(1/4) 547 GAAGAG EcoRII 17 CCAGG ^CC[AT]GG 1146 CCTGG Ksp632I 1124 CTCTTC CTCTTC(1/4) 547 GAAGAG MaeI 625 CTAG C^TAG 1264 CTAG MspA1I 733 CAGCTG C[AC]G^C[GT]G 1249 CAGCTG MvaI 17 CCAGG CC^[AT]GG 1146 CCTGG PvuII 733 CAGCTG CAG^CTG 1249 CAGCTG TfiI 864 GATTC G^A[AT]TC 1120 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AcsI AfaI AluI AlwNI ApoI BbvI BmyI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I Cac8I CfoI DdeI DpnII Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MaeIII MboI MboII MnlI MseI MslI MwoI NdeII NlaIII NlaIV PstI RsaI Sau3AI ScrFI SfaNI SfcI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I