(Linear) (Six-base) MAPSORT of: KIEE2239.seq Check: 6012 from: 1 to: 1299 KIEE2239.orf With 182 enzymes: SgfI * July 15, 2010 18:56 .. Acc65I G'GTAC_C Cuts at: 0 255 1299 Size: 255 1044 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 188 1136 1299 Size: 188 948 163 Fragments arranged by size: 948 188 163 AlwNI CAG_nnn'CTG Cuts at: 0 211 283 307 319 1146 1299 Size: 211 72 24 12 827 153 Fragments arranged by size: 827 211 153 72 24 12 ApaLI G'TGCA_C Cuts at: 0 1009 1299 Size: 1009 290 ApoI r'AATT_y Cuts at: 0 643 1061 1254 1299 Size: 643 418 193 45 Fragments arranged by size: 643 418 193 45 AseI AT'TA_AT Cuts at: 0 32 1299 Size: 32 1267 BanI G'GyrC_C Cuts at: 0 255 267 1299 Size: 255 12 1032 Fragments arranged by size: 1032 255 12 BbsI GAAGACnn'nnnn_ Cuts at: 0 425 1299 Size: 425 874 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 898 932 1299 Size: 898 34 367 Fragments arranged by size: 898 367 34 BlpI GC'TnA_GC Cuts at: 0 1050 1299 Size: 1050 249 Bme1580I G_kGCm'C Cuts at: 0 408 719 1013 1299 Size: 408 311 294 286 Fragments arranged by size: 408 311 294 286 BmrI ACTGGGnnnn_n' Cuts at: 0 586 1299 Size: 586 713 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 773 1299 Size: 773 526 Bpu10I CC'TnA_GC Cuts at: 0 308 320 350 1299 Size: 308 12 30 949 Fragments arranged by size: 949 308 30 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 562 1299 Size: 562 737 BsaI GGTCTCn'nnnn_ Cuts at: 0 655 1299 Size: 655 644 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 360 390 652 682 1299 Size: 360 30 262 30 617 Fragments arranged by size: 617 360 262 30 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1244 1299 Size: 1244 55 BsiHKAI G_wGCw'C Cuts at: 0 1013 1299 Size: 1013 286 Bsp1286I G_dGCh'C Cuts at: 0 408 719 1013 1299 Size: 408 311 294 286 Fragments arranged by size: 408 311 294 286 BsrGI T'GTAC_A Cuts at: 0 668 1299 Size: 668 631 BstXI CCAn_nnnn'nTGG Cuts at: 0 1287 1299 Size: 1287 12 BtsI GCAGTG_nn' Cuts at: 0 746 1299 Size: 746 553 DraI TTT'AAA Cuts at: 0 878 1299 Size: 878 421 EaeI y'GGCC_r Cuts at: 0 965 1299 Size: 965 334 EarI CTCTTCn'nnn_ Cuts at: 0 542 1130 1299 Size: 542 588 169 Fragments arranged by size: 588 542 169 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 188 773 1136 1299 Size: 188 585 363 163 Fragments arranged by size: 585 363 188 163 EcoNI CCTnn'n_nnAGG Cuts at: 0 427 1299 Size: 427 872 EcoRI G'AATT_C Cuts at: 0 1254 1299 Size: 1254 45 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 358 1141 1299 Size: 358 783 158 Fragments arranged by size: 783 358 158 HaeII r_GCGC'y Cuts at: 0 7 1299 Size: 7 1292 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 358 390 1074 1106 1224 1256 1299 Size: 358 32 684 32 118 32 43 Fragments arranged by size: 684 358 118 43 32 32 32 KpnI G_GTAC'C Cuts at: 0 259 1299 Size: 259 1040 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 974 1299 Size: 974 325 MscI TGG'CCA Cuts at: 0 967 1299 Size: 967 332 MslI CAynn'nnrTG Cuts at: 0 399 670 751 1299 Size: 399 271 81 548 Fragments arranged by size: 548 399 271 81 MspA1I CmG'CkG Cuts at: 0 735 1251 1299 Size: 735 516 48 Fragments arranged by size: 735 516 48 NspI r_CATG'y Cuts at: 0 1228 1299 Size: 1228 71 PflMI CCAn_nnn'nTGG Cuts at: 0 796 1299 Size: 796 503 PshAI GACnn'nnGTC Cuts at: 0 425 1299 Size: 425 874 PstI C_TGCA'G Cuts at: 0 170 414 734 1299 Size: 170 244 320 565 Fragments arranged by size: 565 320 244 170 PvuII CAG'CTG Cuts at: 0 735 1251 1299 Size: 735 516 48 Fragments arranged by size: 735 516 48 SapI GCTCTTCn'nnn_ Cuts at: 0 542 1299 Size: 542 757 SfcI C'TryA_G Cuts at: 0 166 410 730 1299 Size: 166 244 320 569 Fragments arranged by size: 569 320 244 166 SmlI C'TyrA_G Cuts at: 0 541 1299 Size: 541 758 SpeI A'CTAG_T Cuts at: 0 1263 1299 Size: 1263 36 SphI G_CATG'C Cuts at: 0 1228 1299 Size: 1228 71 StyI C'CwwG_G Cuts at: 0 1280 1299 Size: 1280 19 TatI w'GTAC_w Cuts at: 0 627 668 1299 Size: 627 41 631 Fragments arranged by size: 631 627 41 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 975 1299 Size: 975 324 XmnI GAAnn'nnTTC Cuts at: 0 1124 1299 Size: 1124 175 Enzymes that do cut: Acc65I AcuI AlwNI ApaLI ApoI AseI BanI BbsI BcgI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaXI BsgI BsiHKAI Bsp1286I BsrGI BstXI BtsI DraI EaeI EarI Eco57MI EcoNI EcoRI FalI HaeII Hin4I KpnI MmeI MscI MslI MspA1I NspI PflMI PshAI PstI PvuII SapI SfcI SmlI SpeI SphI StyI TatI XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI AscI AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbvCI BciVI BclI BfrBI BglI BglII BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoO109I EcoRV FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI XhoI XmaI ZraI