################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvaII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FbaI FseI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 145 GGTACC G^GTACC AcsI 169 AAATTC [GA]^AATT[CT] AfaI 146 GTAC GT^AC AlwNI 12 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 169 AAATTC [GA]^AATT[CT] Asp718I 145 GGTACC G^GTACC AspHI 64 GTGCTC G[AT]GC[AT]^C AviII 293 TGCGCA TGC^GCA BanI 145 GGTACC G^G[CT][GA]CC BanII 262 GAGCCC G[GA]GC[CT]^C BsaWI 164 ACCGGA [AT]^CCGG[AT] BsgI 79 GTGCAG GTGCAG(16/14) BsiHKAI 64 GTGCTC G[AT]GC[AT]^C BsmFI 95 GGGAC GGGAC(10/14) BspMI 290 ACCTGC ACCTGC(4/8) BsrI 254 CCAGT ACTGG(1/-1) BstAPI 11 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 240 GGTCACC G^GT[ACGT]ACC BstF5I 42 CATCC GGATG(2/0) BstNI 149 CCAGG CC^[AT]GG BstPI 240 GGTCACC G^GT[ACGT]ACC BstYI 219 GGATCT [GA]^GATC[CT] CfoI 294 GCGC GCG^C Cfr13I 38 GGGCC G^G[ACGT]CC DsaI 269 CCATGG C^C[GA][CT]GG EcoO65I 240 GGTCACC G^GT[ACGT]ACC EcoRII 149 CCAGG ^CC[AT]GG FokI 42 CATCC GGATG(9/13) FspI 293 TGCGCA TGC^GCA HaeIII 39 GGCC GG^CC HhaI 294 GCGC GCG^C HinP1I 294 GCGC G^CGC HinfI 331 GAGTC G^A[ACGT]TC KpnI 145 GGTACC GGTAC^C MflI 219 GGATCT [GA]^GATC[CT] MnlI 143 GAGG CCTC(7/6) MspA1I 15 CAGCTG C[AC]G^C[GT]G MvaI 149 CCAGG CC^[AT]GG NcoI 269 CCATGG C^CATGG NlaIII 270 CATG CATG^ NlaIV 145 GGTACC GG[ACGT]^[ACGT]CC PleI 331 GAGTC GAGTC(4/5) PstI 18 CTGCAG CTGCA^G PvuII 15 CAGCTG CAG^CTG RsaI 146 GTAC GT^AC SapI 123 GCTCTTC GCTCTTC(1/4) Sau96I 38 GGGCC G^G[ACGT]CC Tsp509I 170 AATT ^AATT TspRI 255 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 50 CCACTCGCCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 219 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AluI 16 AGCT AG^CT 83 AGCT AlwI 152 GGATC GGATC(4/5) 219 GGATC AvaI 91 CCCGGG C^[CT]CG[GA]G 138 CCCGGG BmyI 64 GTGCTC G[AGT]GC[ACT]^C 262 GAGCCC BsoBI 91 CCCGGG C^[CT]CG[GA]G 138 CCCGGG Bsp1286I 64 GTGCTC G[AGT]GC[ACT]^C 262 GAGCCC DpnII 153 GATC ^GATC 220 GATC EarI 124 CTCTTC CTCTTC(1/4) 328 GAAGAG EcoT14I 214 CCAAGG C^C[AT][AT]GG 269 CCATGG Ksp632I 124 CTCTTC CTCTTC(1/4) 328 GAAGAG MboI 153 GATC ^GATC 220 GATC NdeII 153 GATC ^GATC 220 GATC Sau3AI 153 GATC ^GATC 220 GATC SfcI 18 CTGCAG C^T[GA][CT]AG 99 CTACAG SmaI 91 CCCGGG CCC^GGG 138 CCCGGG StyI 214 CCAAGG C^C[AT][AT]GG 269 CCATGG XmaCI 91 CCCGGG C^CCGGG 138 CCCGGG XmaI 91 CCCGGG C^CCGGG 138 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AciI BbvI BcnI BsaJI BsiYI BslI BssKI Cac8I FauI Fnu4HI HapII HpaII HphI ItaI MaeIII MboII MspI MwoI NciI ScrFI TseI Tsp45I