(Linear) (Six-base) MAPSORT of: KIEE1520.seq Check: 6902 from: 1 to: 354 KIEE1520.orf With 182 enzymes: SgfI * July 15, 2010 18:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 299 354 Size: 299 55 Acc65I G'GTAC_C Cuts at: 0 145 354 Size: 145 209 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 111 354 Size: 111 243 AlwNI CAG_nnn'CTG Cuts at: 0 17 354 Size: 17 337 ApoI r'AATT_y Cuts at: 0 169 354 Size: 169 185 AvaI C'yCGr_G Cuts at: 0 91 138 354 Size: 91 47 216 Fragments arranged by size: 216 91 47 BanI G'GyrC_C Cuts at: 0 145 354 Size: 145 209 BanII G_rGCy'C Cuts at: 0 266 354 Size: 266 88 BsaWI w'CCGG_w Cuts at: 0 164 354 Size: 164 190 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 354 Size: 100 254 BsiHKAI G_wGCw'C Cuts at: 0 68 354 Size: 68 286 Bsp1286I G_dGCh'C Cuts at: 0 68 266 354 Size: 68 198 88 Fragments arranged by size: 198 88 68 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 299 354 Size: 299 55 BstAPI GCAn_nnn'nTGC Cuts at: 0 17 354 Size: 17 337 BstEII G'GTnAC_C Cuts at: 0 240 354 Size: 240 114 BstYI r'GATC_y Cuts at: 0 219 354 Size: 219 135 BtgI C'CryG_G Cuts at: 0 269 354 Size: 269 85 EarI CTCTTCn'nnn_ Cuts at: 0 130 323 354 Size: 130 193 31 Fragments arranged by size: 193 130 31 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 111 354 Size: 111 243 FspI TGC'GCA Cuts at: 0 295 354 Size: 295 59 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 168 200 354 Size: 168 32 154 Fragments arranged by size: 168 154 32 KpnI G_GTAC'C Cuts at: 0 149 354 Size: 149 205 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 166 354 Size: 166 188 MspA1I CmG'CkG Cuts at: 0 17 354 Size: 17 337 NcoI C'CATG_G Cuts at: 0 269 354 Size: 269 85 PstI C_TGCA'G Cuts at: 0 22 354 Size: 22 332 PvuII CAG'CTG Cuts at: 0 17 354 Size: 17 337 SapI GCTCTTCn'nnn_ Cuts at: 0 130 354 Size: 130 224 SfcI C'TryA_G Cuts at: 0 18 99 354 Size: 18 81 255 Fragments arranged by size: 255 81 18 SmaI CCC'GGG Cuts at: 0 93 140 354 Size: 93 47 214 Fragments arranged by size: 214 93 47 StyI C'CwwG_G Cuts at: 0 214 269 354 Size: 214 55 85 Fragments arranged by size: 214 85 55 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 57 354 Size: 57 297 XmaI C'CCGG_G Cuts at: 0 91 138 354 Size: 91 47 216 Fragments arranged by size: 216 91 47 Enzymes that do cut: AarI Acc65I AcuI AlwNI ApoI AvaI BanI BanII BsaWI BsgI BsiHKAI Bsp1286I BspMI BstAPI BstEII BstYI BtgI EarI Eco57MI FspI Hin4I KpnI MmeI MspA1I NcoI PstI PvuII SapI SfcI SmaI StyI XcmI XmaI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI MscI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmnI ZraI