################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII Acc65I AccI AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsiWI BspDI BsrDI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HpaI KpnI KspI MamI MfeI MluI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SexAI SfuI SgrAI SmlI SnaBI SpeI SspI SwaI Tru9I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccIII 356 TCCGGA T^CCGGA AcyI 945 GGCGCC G[GA]^CG[CT]C Asp700I 572 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbeI 945 GGCGCC GGCGC^C BfaI 34 CTAG C^TAG BglII 89 AGATCT A^GATCT BsaAI 852 CACGTA [CT]AC^GT[GA] BsaHI 945 GGCGCC G[GA]^CG[CT]C BseAI 356 TCCGGA T^CCGGA BsiEI 1310 CGGCCG CG[GA][CT]^CG BsmI 493 GAATGC GAATGC(1/-1) Bsp1407I 1820 TGTACA T^GTACA BspEI 356 TCCGGA T^CCGGA BspLU11I 1904 ACATGT A^CATGT BspMI 281 ACCTGC ACCTGC(4/8) BsrBI 1624 GAGCGG CCGCTC(-3/-3) BsrGI 1820 TGTACA T^GTACA BssSI 1213 CACGAG CACGAG(-5/-1) BstAPI 124 GCAAAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraIII 1813 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1310 CGGCCG C^GGCCG EclXI 1310 CGGCCG C^GGCCG Eco52I 1310 CGGCCG C^GGCCG Hin1I 945 GGCGCC G[GA]^CG[CT]C HincII 157 GTCAAC GT[CT]^[GA]AC HindII 157 GTCAAC GT[CT]^[GA]AC HindIII 1480 AAGCTT A^AGCTT KasI 945 GGCGCC G^GCGCC MaeI 34 CTAG C^TAG MroI 356 TCCGGA T^CCGGA NarI 945 GGCGCC GG^CGCC PpuMI 278 AGGACCT [GA]G^G[AT]CC[CT] PshAI 842 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 973 GAGCTC GAGCT^C SapI 1465 GAAGAGC GCTCTTC(1/4) ScaI 587 AGTACT AGT^ACT SfoI 945 GGCGCC GGC^GCC SmaI 1439 CCCGGG CCC^GGG SspBI 1820 TGTACA T^GTACA StuI 338 AGGCCT AGG^CCT XmaCI 1439 CCCGGG C^CCGGG XmaI 1439 CCCGGG C^CCGGG XmnI 572 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AcsI 138 GAATTC [GA]^AATT[CT] 430 GAATTC AflIII 174 ACACGT A^C[GA][CT]GT 1904 ACATGT ApoI 138 GAATTC [GA]^AATT[CT] 430 GAATTC BsaWI 356 TCCGGA [AT]^CCGG[AT] 992 ACCGGA BssHII 1668 GCGCGC G^CGCGC 1670 GCGCGC DraII 278 AGGACCT [GA]G^G[ACGT]CC[CT] 2030 AGGCCCC EcoO109I 278 AGGACCT [GA]G^G[ACGT]CC[CT] 2030 AGGCCCC EcoRI 138 GAATTC G^AATTC 430 GAATTC SbfI 768 CCTGCAGG CCTGCA^GG 1605 CCTGCAGG SfiI 1938 GGCCCCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 2022 GGCCTCCCAGGCC Sse8387I 768 CCTGCAGG CCTGCA^GG 1605 CCTGCAGG TfiI 205 GAATC G^A[AT]TC 2136 GAATC Tsp45I 807 GTCAC ^GT[GC]AC 1246 GTCAC Tsp509I 139 AATT ^AATT 431 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspHI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EarI EcoNI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SphI StyI TaqI TseI TspRI TthHB8I Van91I XcmI XhoII