################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsiEI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HaeII HgaI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MscI MseI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AccIII 783 TCCGGA T^CCGGA AfaI 138 GTAC GT^AC AlwI 38 GATCC GGATC(4/5) BsaWI 783 TCCGGA [AT]^CCGG[AT] BseAI 783 TCCGGA T^CCGGA BsmBI 714 CGTCTC CGTCTC(1/5) BspEI 783 TCCGGA T^CCGGA BsrDI 249 CATTGC GCAATG(2/0) BssHII 315 GCGCGC G^CGCGC BstXI 255 CCACCTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 37 AGATCC [GA]^GATC[CT] EaeI 800 TGGCCG [CT]^GGCC[GA] FauI 200 GCGGG CCCGC(4/6) HinfI 468 GAGTC G^A[ACGT]TC MboII 680 GAAGA GAAGA(8/7) MflI 37 AGATCC [GA]^GATC[CT] MroI 783 TCCGGA T^CCGGA NcoI 305 CCATGG C^CATGG NdeI 666 CATATG CA^TATG NlaIII 306 CATG CATG^ PleI 468 GAGTC GAGTC(4/5) PvuII 555 CAGCTG CAG^CTG RsaI 138 GTAC GT^AC SbfI 771 CCTGCAGG CCTGCA^GG SfaNI 380 GCATC GCATC(5/9) SfiI 768 GGCCCTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 60 CTTGAG C^T[CT][GA]AG Sse8387I 771 CCTGCAGG CCTGCA^GG StuI 194 AGGCCT AGG^CCT XhoII 37 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AatII 55 GACGTC GACGT^C 787 GACGTC AcyI 55 GACGTC G[GA]^CG[CT]C 787 GACGTC AvaI 435 CCCGGG C^[CT]CG[GA]G 498 CCCGGG BanI 241 GGCACC G^G[CT][GA]CC 460 GGCACC BglI 233 GCCCTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 769 GCCCTGCAGGC BsaHI 55 GACGTC G[GA]^CG[CT]C 787 GACGTC BsoBI 435 CCCGGG C^[CT]CG[GA]G 498 CCCGGG DdeI 268 CTAAG C^T[ACGT]AG 618 CTCAG DpnII 38 GATC ^GATC 711 GATC DraIII 216 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG 477 CACCCTGTG EcoT14I 305 CCATGG C^C[AT][AT]GG 688 CCAAGG Hin1I 55 GACGTC G[GA]^CG[CT]C 787 GACGTC MaeII 56 ACGT A^CGT 788 ACGT MboI 38 GATC ^GATC 711 GATC MspA1I 555 CAGCTG C[AC]G^C[GT]G 724 CAGCGG NdeII 38 GATC ^GATC 711 GATC PpuMI 502 GGGACCT [GA]G^G[AT]CC[CT] 520 GGGACCC Sau3AI 38 GATC ^GATC 711 GATC SmaI 435 CCCGGG CCC^GGG 498 CCCGGG StyI 305 CCATGG C^C[AT][AT]GG 688 CCAAGG XcmI 515 CCAATGGGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 794 CCACGGTGGCCGTGG XmaCI 435 CCCGGG C^CCGGG 498 CCCGGG XmaI 435 CCCGGG C^CCGGG 498 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AccII AciI AluI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DraII DsaI EcoO109I EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MnlI MslI MspI MvaI MvnI MwoI NciI NlaIV PstI Sau96I ScrFI SfcI TseI Tsp45I TspRI