(Circular) (Six-base) MAPSORT of: pFN21AE1501.seq Check: 60 from: 1 to: 5554 pFN21AE1501 5554 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2064 2796 3364 278 Size: 53 83 186 936 528 732 568 2468 Fragments arranged by size: 2468 936 732 568 528 186 83 53 AccI GT'mk_AC Cuts at: 1937 2897 1937 Size: 960 4594 Acc65I G'GTAC_C Cuts at: 2875 2875 Size: 5554 AclI AA'CG_TT Cuts at: 1563 3681 4054 1563 Size: 2118 373 3063 Fragments arranged by size: 3063 2118 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3617 5001 5269 1632 Size: 1985 1384 268 1917 Fragments arranged by size: 1985 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5357 AflIII A'CryG_T Cuts at: 1203 4453 5430 1203 Size: 3250 977 1327 Fragments arranged by size: 3250 1327 977 AgeI A'CCGG_T Cuts at: 4385 4385 Size: 5554 AhdI GACnn_n'nnGTC Cuts at: 4283 4283 Size: 5554 AleI CACnn'nnGTG Cuts at: 1166 2801 1166 Size: 1635 3919 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2300 1184 Size: 1116 4438 AlwNI CAG_nnn'CTG Cuts at: 3297 4869 3297 Size: 1572 3982 ApaLI G'TGCA_C Cuts at: 3611 4767 3611 Size: 1156 4398 ApoI r'AATT_y Cuts at: 1493 2863 3089 3241 5350 1493 Size: 1370 226 152 2109 1697 Fragments arranged by size: 2109 1697 1370 226 152 AseI AT'TA_AT Cuts at: 160 1032 4108 160 Size: 872 3076 1606 Fragments arranged by size: 3076 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5554 AvaI C'yCGr_G Cuts at: 1870 1943 2440 2503 2879 1870 Size: 73 497 63 376 4545 Fragments arranged by size: 4545 497 376 73 63 BamHI G'GATC_C Cuts at: 2884 3251 2884 Size: 367 5187 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2246 2465 2875 4330 618 Size: 325 217 627 459 219 410 1455 1842 Fragments arranged by size: 1842 1455 627 459 410 325 219 217 BanII G_rGCy'C Cuts at: 1391 2101 2156 2846 2873 1391 Size: 710 55 690 27 4072 Fragments arranged by size: 4072 710 690 55 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4583 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3745 3779 1903 Size: 34 1808 34 3678 Fragments arranged by size: 3678 1808 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3446 4656 3446 Size: 1210 4344 BclI T'GATC_A Cuts at: 1548 1548 Size: 5554 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2244 2780 4165 Size: 107 122 71 631 605 572 536 1385 Cuts at: 4165 136 Size: 1525 Fragments arranged by size: 1525 1385 631 605 572 536 122 107 71 BglII A'GATC_T Cuts at: 5549 5549 Size: 5554 BlpI GC'TnA_GC Cuts at: 2971 2971 Size: 5554 Bme1580I G_kGCm'C Cuts at: 1790 2222 3615 4771 1790 Size: 432 1393 1156 2573 Fragments arranged by size: 2573 1393 1156 432 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5554 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2032 2141 2614 Size: 416 53 83 399 24 615 109 473 Cuts at: 2614 3285 4243 442 Size: 671 958 1753 Fragments arranged by size: 1753 958 671 615 473 416 399 109 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5554 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2037 2059 2226 2323 2419 2552 4214 1239 Size: 798 22 167 97 96 133 1662 2579 Fragments arranged by size: 2579 1662 798 167 133 97 96 22 Bpu10I CC'TnA_GC Cuts at: 2273 2273 Size: 5554 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2086 3679 4544 4842 2086 Size: 1593 865 298 2798 Fragments arranged by size: 2798 1593 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4217 882 Size: 617 2718 2219 Fragments arranged by size: 2718 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3345 493 Size: 711 2141 2702 Fragments arranged by size: 2702 2141 711 BsaBI GATnn'nnATC Cuts at: 5548 5548 Size: 5554 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2061 2793 3361 3743 Size: 53 83 186 936 528 732 568 382 Cuts at: 3743 4378 275 Size: 635 1451 Fragments arranged by size: 1451 936 732 635 568 528 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2788 3987 4385 4659 4806 5217 2788 Size: 1199 398 274 147 411 3125 Fragments arranged by size: 3125 1199 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2126 2156 2417 2447 2500 Size: 30 165 30 723 30 261 30 53 Cuts at: 2500 2530 1178 Size: 30 4202 Fragments arranged by size: 4202 723 261 165 53 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2022 2828 2854 1186 Size: 836 806 26 3886 Fragments arranged by size: 3886 836 806 26 BseYI C'CCAG_C Cuts at: 2113 2305 2585 4757 5253 2113 Size: 192 280 2172 496 2414 Fragments arranged by size: 2414 2172 496 280 192 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2194 2474 2578 1163 Size: 573 458 280 104 4139 Fragments arranged by size: 4139 573 458 280 104 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3004 3765 4793 1540 Size: 275 186 1003 761 1028 2301 Fragments arranged by size: 2301 1028 1003 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3615 3700 4771 3615 Size: 85 1071 4398 Fragments arranged by size: 4398 1071 85 BsmI GAATG_Cn' Cuts at: 3065 3158 3065 Size: 93 5461 BsmBI CGTCTCn'nnnn_ Cuts at: 2725 2725 Size: 5554 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2101 2156 2222 2846 2873 3615 3700 Size: 399 311 55 66 624 27 742 85 Cuts at: 3700 4771 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 624 399 311 85 66 55 27 BspEI T'CCGG_A Cuts at: 2788 5217 2788 Size: 2429 3125 BspHI T'CATG_A Cuts at: 3443 5173 3443 Size: 1730 3824 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2909 844 Size: 2065 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3441 1394 Size: 429 1618 3507 Fragments arranged by size: 3507 1618 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2253 4049 4223 65 Size: 1183 1005 1796 174 1396 Fragments arranged by size: 1796 1396 1183 1005 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4198 4385 1700 Size: 110 2388 187 2869 Fragments arranged by size: 2869 2388 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5554 BssHII G'CGCG_C Cuts at: 1925 2320 1925 Size: 395 5159 BssSI C'ACGA_G Cuts at: 3614 4626 3614 Size: 1012 4542 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5554 BstBI TT'CG_AA Cuts at: 4358 4358 Size: 5554 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5554 BstXI CCAn_nnnn'nTGG Cuts at: 2267 2267 Size: 5554 BstYI r'GATC_y Cuts at: 1972 2042 2884 3251 3636 3653 5105 5191 5203 Size: 70 842 367 385 17 1452 86 12 Cuts at: 5203 5549 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 842 385 367 346 86 70 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2310 2799 2808 513 Size: 552 939 306 489 9 3259 Fragments arranged by size: 3259 939 552 489 306 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4815 Fragments arranged by size: 4815 683 56 BtsI GCAGTG_nn' Cuts at: 2294 2404 3070 3864 3884 2294 Size: 110 666 794 20 3964 Fragments arranged by size: 3964 794 666 110 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5554 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2749 2784 529 Size: 35 2185 35 3299 Fragments arranged by size: 3299 2185 35 35 DraI TTT'AAA Cuts at: 2858 2858 Size: 5554 DraIII CAC_nnn'GTG Cuts at: 2226 2487 2226 Size: 261 5293 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4561 809 Size: 507 3245 1802 Fragments arranged by size: 3245 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2805 3001 3890 Size: 54 1425 50 275 18 975 196 889 Cuts at: 3890 5519 8 Size: 1629 43 Fragments arranged by size: 1629 1425 975 889 275 196 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3001 1537 Size: 275 1189 4090 Fragments arranged by size: 4090 1189 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3484 1353 Size: 2131 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4141 4515 4661 387 Size: 1064 138 2552 374 146 1280 Fragments arranged by size: 2552 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2037 2059 2226 2323 2419 2552 3617 Size: 393 405 22 167 97 96 133 1065 Cuts at: 3617 4214 5001 5269 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1065 787 597 405 393 268 167 133 97 96 22 EcoO109I rG'GnC_Cy Cuts at: 2027 2508 2526 2773 2782 2027 Size: 481 18 247 9 4799 Fragments arranged by size: 4799 481 247 18 9 EcoRI G'AATT_C Cuts at: 2863 3241 2863 Size: 378 5176 FspI TGC'GCA Cuts at: 4060 4060 Size: 5554 HaeII r_GCGC'y Cuts at: 1678 4701 1678 Size: 3023 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2500 2532 4195 4227 4269 Size: 32 135 32 928 32 1663 32 42 Cuts at: 4269 4301 1373 Size: 32 2626 Fragments arranged by size: 2626 1663 928 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2898 3144 669 Size: 988 281 960 246 3079 Fragments arranged by size: 3079 988 960 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5554 HpaI GTT'AAC Cuts at: 3144 3144 Size: 5554 KpnI G_GTAC'C Cuts at: 2879 2879 Size: 5554 MfeI C'AATT_G Cuts at: 3153 3153 Size: 5554 MluI A'CGCG_T Cuts at: 5430 5430 Size: 5554 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4668 4852 1305 Size: 3363 184 2007 Fragments arranged by size: 3363 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3732 Fragments arranged by size: 3732 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2265 2675 2801 3512 3871 4030 518 Size: 648 1099 410 126 711 359 159 2042 Fragments arranged by size: 2042 1099 711 648 410 359 159 126 MspA1I CmG'CkG Cuts at: 1606 2562 2731 2970 3647 4795 5040 1606 Size: 956 169 239 677 1148 245 2120 Fragments arranged by size: 2120 1148 956 677 245 239 169 NcoI C'CATG_G Cuts at: 513 1065 2004 2310 513 Size: 552 939 306 3757 Fragments arranged by size: 3757 939 552 306 NdeI CA'TA_TG Cuts at: 387 2672 387 Size: 2285 3269 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5554 NotI GC'GGCC_GC Cuts at: 3001 3001 Size: 5554 NspI r_CATG'y Cuts at: 1132 1229 2912 4457 1132 Size: 97 1683 1545 2229 Fragments arranged by size: 2229 1683 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5554 PciI A'CATG_T Cuts at: 4453 4453 Size: 5554 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5554 PfoI T'CCnGG_A Cuts at: 2014 2339 2391 2014 Size: 325 52 5177 Fragments arranged by size: 5177 325 52 PmeI GTTT'AAAC Cuts at: 2858 2858 Size: 5554 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3623 3655 5136 5168 3623 Size: 32 1481 32 4009 Fragments arranged by size: 4009 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2508 2526 2508 Size: 18 5536 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5554 PsiI TTA'TAA Cuts at: 3124 3124 Size: 5554 PstI C_TGCA'G Cuts at: 830 2289 2369 2781 2906 830 Size: 1459 80 412 125 3478 Fragments arranged by size: 3478 1459 412 125 80 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5554 PvuII CAG'CTG Cuts at: 2562 2562 Size: 5554 SalI G'TCGA_C Cuts at: 1936 2896 1936 Size: 960 4594 SanDI GG'GwC_CC Cuts at: 2526 2526 Size: 5554 SbfI CC_TGCA'GG Cuts at: 2781 2906 2781 Size: 125 5429 ScaI AGT'ACT Cuts at: 3802 5514 3802 Size: 1712 3842 SfcI C'TryA_G Cuts at: 826 1045 2285 2365 2777 2902 4037 4718 4909 Size: 219 1240 80 412 125 1135 681 191 Cuts at: 4909 826 Size: 1471 Fragments arranged by size: 1471 1240 1135 681 412 219 191 125 80 SfiI GGCCn_nnn'nGGCC Cuts at: 2780 2780 Size: 5554 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5554 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5554 SmaI CCC'GGG Cuts at: 1872 2442 2505 2881 1872 Size: 570 63 376 4545 Fragments arranged by size: 4545 570 376 63 SmlI C'TyrA_G Cuts at: 820 1017 1943 2065 3658 4559 4821 820 Size: 197 926 122 1593 901 262 1553 Fragments arranged by size: 1593 1553 926 901 262 197 122 SnaBI TAC'GTA Cuts at: 493 3345 493 Size: 2852 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5554 SphI G_CATG'C Cuts at: 1132 2912 1132 Size: 1780 3774 SrfI GCCC'GGGC Cuts at: 2442 2442 Size: 5554 SspI AAT'ATT Cuts at: 5 52 3478 5 Size: 47 3426 2081 Fragments arranged by size: 3426 2081 47 StuI AGG'CCT Cuts at: 1517 2201 1517 Size: 684 4870 StyI C'CwwG_G Cuts at: 513 1065 2004 2310 2693 2993 513 Size: 552 939 306 383 300 3074 Fragments arranged by size: 3074 939 552 383 306 300 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3581 3598 3751 3936 4381 325 Size: 1255 2001 17 153 185 445 1498 Fragments arranged by size: 2001 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3800 5512 96 Size: 274 80 33 51 1443 1823 1712 138 Fragments arranged by size: 1823 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2471 2503 5514 5546 2471 Size: 32 3011 32 2479 Fragments arranged by size: 3011 2479 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5554 XbaI T'CTAG_A Cuts at: 2890 2890 Size: 5554 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2527 2806 2527 Size: 279 5275 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5554 XmaI C'CCGG_G Cuts at: 1870 2440 2503 2879 1870 Size: 570 63 376 4545 Fragments arranged by size: 4545 570 376 63 XmnI GAAnn'nnTTC Cuts at: 3683 3683 Size: 5554 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2062 2794 3362 276 Size: 53 83 186 936 528 732 568 2468 Fragments arranged by size: 2468 936 732 568 528 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfoI SwaI