(Circular) (Six-base) MAPSORT of: pFN21AB9961.seq Check: 3707 from: 1 to: 6313 pFN21AB9961 6313 bp With 182 enzymes: SgfI * May 28, 2009 10:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4123 278 Size: 53 83 186 936 2587 2468 Fragments arranged by size: 2587 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3656 1937 Size: 1719 4594 Acc65I G'GTAC_C Cuts at: 2876 3634 2876 Size: 758 5555 AclI AA'CG_TT Cuts at: 1563 4440 4813 1563 Size: 2877 373 3063 Fragments arranged by size: 3063 2877 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2924 4376 5760 6028 1632 Size: 1292 1452 1384 268 1917 Fragments arranged by size: 1917 1452 1384 1292 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6116 AflIII A'CryG_T Cuts at: 1203 3345 5212 6189 1203 Size: 2142 1867 977 1327 Fragments arranged by size: 2142 1867 1327 977 AgeI A'CCGG_T Cuts at: 5144 5144 Size: 6313 AhdI GACnn_n'nnGTC Cuts at: 2290 3349 5042 2290 Size: 1059 1693 3561 Fragments arranged by size: 3561 1693 1059 AleI CACnn'nnGTG Cuts at: 1166 2270 2741 2885 1166 Size: 1104 471 144 4594 Fragments arranged by size: 4594 1104 471 144 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3006 3101 1184 Size: 1822 95 4396 Fragments arranged by size: 4396 1822 95 AlwNI CAG_nnn'CTG Cuts at: 2579 2689 2776 3311 4056 5628 2579 Size: 110 87 535 745 1572 3264 Fragments arranged by size: 3264 1572 745 535 110 87 ApaI G_GGCC'C Cuts at: 2246 2246 Size: 6313 ApaLI G'TGCA_C Cuts at: 2075 4370 5526 2075 Size: 2295 1156 2862 Fragments arranged by size: 2862 2295 1156 ApoI r'AATT_y Cuts at: 1493 3281 3622 3848 4000 6109 1493 Size: 1788 341 226 152 2109 1697 Fragments arranged by size: 2109 1788 1697 341 226 152 AseI AT'TA_AT Cuts at: 160 1032 4867 160 Size: 872 3835 1606 Fragments arranged by size: 3835 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 AvaI C'yCGr_G Cuts at: 1870 1943 2515 2802 3476 3599 3638 1870 Size: 73 572 287 674 123 39 4545 Fragments arranged by size: 4545 674 572 287 123 73 39 BamHI G'GATC_C Cuts at: 3360 3643 4010 3360 Size: 283 367 5663 Fragments arranged by size: 5663 367 283 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2046 2425 2558 2876 3275 Size: 325 217 627 259 379 133 318 399 Cuts at: 3275 3394 3634 5089 618 Size: 119 240 1455 1842 Fragments arranged by size: 1842 1455 627 399 379 325 318 259 240 217 133 119 BanII G_rGCy'C Cuts at: 1391 2064 2187 2246 3012 3378 3588 3632 1391 Size: 673 123 59 766 366 210 44 4072 Fragments arranged by size: 4072 766 673 366 210 123 59 44 BbeI G_GCGC'C Cuts at: 2050 2429 2050 Size: 379 5934 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3255 928 Size: 971 1356 3986 Fragments arranged by size: 3986 1356 971 BbvCI CC'TCA_GC Cuts at: 2448 2963 3096 2448 Size: 515 133 5665 Fragments arranged by size: 5665 515 133 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4504 4538 1903 Size: 34 2567 34 3678 Fragments arranged by size: 3678 2567 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4205 5415 4205 Size: 1210 5103 BclI T'GATC_A Cuts at: 1548 1548 Size: 6313 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2148 2174 4924 Size: 107 122 71 631 605 476 26 2750 Cuts at: 4924 136 Size: 1525 Fragments arranged by size: 2750 1525 631 605 476 122 107 71 26 BglII A'GATC_T Cuts at: 6308 6308 Size: 6313 BlpI GC'TnA_GC Cuts at: 2384 3730 2384 Size: 1346 4967 Bme1580I G_kGCm'C Cuts at: 1790 2079 2246 2750 3399 4374 5530 1790 Size: 289 167 504 649 975 1156 2573 Fragments arranged by size: 2573 1156 975 649 504 289 167 BmgBI CAC'GTC Cuts at: 1318 2754 3346 3576 1318 Size: 1436 592 230 4055 Fragments arranged by size: 4055 1436 592 230 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2662 3583 4044 Size: 416 53 83 399 24 1245 921 461 Cuts at: 4044 5002 442 Size: 958 1753 Fragments arranged by size: 1753 1245 958 921 461 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6313 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2078 2321 2840 2907 3288 3581 4973 1239 Size: 839 243 519 67 381 293 1392 2579 Fragments arranged by size: 2579 1392 839 519 381 293 243 67 Bpu10I CC'TnA_GC Cuts at: 2448 2963 3096 2448 Size: 515 133 5665 Fragments arranged by size: 5665 515 133 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3047 4438 5303 5601 3047 Size: 1391 865 298 3759 Fragments arranged by size: 3759 1391 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2540 4976 882 Size: 617 1041 2436 2219 Fragments arranged by size: 2436 2219 1041 617 BsaAI yAC'GTr Cuts at: 493 1204 2422 3142 4104 493 Size: 711 1218 720 962 2702 Fragments arranged by size: 2702 1218 962 720 711 BsaBI GATnn'nnATC Cuts at: 6307 6307 Size: 6313 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2047 2426 4120 4502 Size: 53 83 186 936 514 379 1694 382 Cuts at: 4502 5137 275 Size: 635 1451 Fragments arranged by size: 1694 1451 936 635 514 382 379 186 83 53 BsaWI w'CCGG_w Cuts at: 3450 4746 5144 5418 5565 5976 3450 Size: 1296 398 274 147 411 3787 Fragments arranged by size: 3787 1296 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2642 2672 2905 2935 1178 Size: 30 165 30 1239 30 233 30 4556 Fragments arranged by size: 4556 1239 233 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2200 2465 3020 3539 1186 Size: 1014 265 555 519 3960 Fragments arranged by size: 3960 1014 555 519 265 BseYI C'CCAG_C Cuts at: 2117 2153 2411 2830 5516 6012 2117 Size: 36 258 419 2686 496 2418 Fragments arranged by size: 2686 2418 496 419 258 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3002 3198 3470 1163 Size: 573 1266 196 272 4006 Fragments arranged by size: 4006 1266 573 272 196 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2136 2178 3763 4524 5552 1540 Size: 275 186 135 42 1585 761 1028 2301 Fragments arranged by size: 2301 1585 1028 761 275 186 135 42 BsiHKAI G_wGCw'C Cuts at: 2079 3378 4374 4459 5530 2079 Size: 1299 996 85 1071 2862 Fragments arranged by size: 2862 1299 1071 996 85 BsiWI C'GTAC_G Cuts at: 3138 3138 Size: 6313 BsmI GAATG_Cn' Cuts at: 3824 3917 3824 Size: 93 6220 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2079 2187 2246 2750 3012 3378 Size: 399 274 15 108 59 504 262 366 Cuts at: 3378 3399 3588 3632 4374 4459 5530 1391 Size: 21 189 44 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 504 399 366 274 262 189 108 85 59 44 21 15 BspEI T'CCGG_A Cuts at: 5976 5976 Size: 6313 BspHI T'CATG_A Cuts at: 2952 4202 5932 2952 Size: 1250 1730 3333 Fragments arranged by size: 3333 1730 1250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3093 3555 3668 844 Size: 2249 462 113 3489 Fragments arranged by size: 3489 2249 462 113 BsrBI CCG'CTC Cuts at: 1394 1823 2332 2491 3148 3442 3529 4200 1394 Size: 429 509 159 657 294 87 671 3507 Fragments arranged by size: 3507 671 657 509 429 294 159 87 BsrDI GCAATG_nn' Cuts at: 65 1248 4808 4982 65 Size: 1183 3560 174 1396 Fragments arranged by size: 3560 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2252 4957 5144 1700 Size: 110 442 2705 187 2869 Fragments arranged by size: 2869 2705 442 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6313 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6313 BssSI C'ACGA_G Cuts at: 2531 4373 5385 2531 Size: 1842 1012 3459 Fragments arranged by size: 3459 1842 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6313 BstBI TT'CG_AA Cuts at: 5117 5117 Size: 6313 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6313 BstXI CCAn_nnnn'nTGG Cuts at: 2272 2887 3190 2272 Size: 615 303 5395 Fragments arranged by size: 5395 615 303 BstYI r'GATC_y Cuts at: 1972 2622 2796 3360 3643 4010 4395 4412 5864 Size: 650 174 564 283 367 385 17 1452 Cuts at: 5864 5950 5962 6308 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 650 564 385 367 346 283 174 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3127 3522 3127 Size: 395 5918 BtgI C'CryG_G Cuts at: 513 1065 2004 2146 2172 513 Size: 552 939 142 26 4654 Fragments arranged by size: 4654 939 552 142 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5574 Fragments arranged by size: 5574 683 56 BtsI GCAGTG_nn' Cuts at: 3829 4623 4643 3829 Size: 794 20 5499 Fragments arranged by size: 5499 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6313 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6278 DraI TTT'AAA Cuts at: 3617 3617 Size: 6313 DraIII CAC_nnn'GTG Cuts at: 2745 2745 Size: 6313 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2311 5320 809 Size: 507 995 3009 1802 Fragments arranged by size: 3009 1802 995 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 2140 2175 Size: 54 1425 50 275 18 303 7 35 Cuts at: 2175 2250 2778 2889 3219 3322 3760 4649 6278 Size: 75 528 111 330 103 438 889 1629 Cuts at: 6278 8 Size: 43 Fragments arranged by size: 1629 1425 889 528 438 330 303 275 111 103 75 54 50 43 35 18 7 EagI C'GGCC_G Cuts at: 1537 1812 2133 2175 3760 1537 Size: 275 321 42 1585 4090 Fragments arranged by size: 4090 1585 321 275 42 EarI CTCTTCn'nnn_ Cuts at: 1353 4243 1353 Size: 2890 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3526 4900 5274 5420 387 Size: 1064 138 1937 1374 374 146 1280 Fragments arranged by size: 1937 1374 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3376 3376 Size: 6313 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2321 2840 2907 2924 3288 3581 Size: 393 446 243 519 67 17 364 293 Cuts at: 3581 4376 4973 5760 6028 1239 Size: 795 597 787 268 1524 Fragments arranged by size: 1524 795 787 597 519 446 393 364 293 268 243 67 17 EcoNI CCTnn'n_nnAGG Cuts at: 2780 2939 2780 Size: 159 6154 EcoO109I rG'GnC_Cy Cuts at: 2523 2599 2773 2865 3169 2523 Size: 76 174 92 304 5667 Fragments arranged by size: 5667 304 174 92 76 EcoRI G'AATT_C Cuts at: 3622 4000 3622 Size: 378 5935 FspI TGC'GCA Cuts at: 4819 4819 Size: 6313 HaeII r_GCGC'y Cuts at: 1678 2050 2085 2097 2429 5460 1678 Size: 372 35 12 332 3031 2531 Fragments arranged by size: 3031 2531 372 332 35 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2276 2308 3335 3367 4954 Size: 32 135 32 704 32 1027 32 1587 Cuts at: 4954 4986 5028 5060 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1587 1027 704 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2347 3657 3903 669 Size: 988 281 409 1310 246 3079 Fragments arranged by size: 3079 1310 988 409 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6313 HpaI GTT'AAC Cuts at: 3903 3903 Size: 6313 KasI G'GCGC_C Cuts at: 2046 2425 2046 Size: 379 5934 KpnI G_GTAC'C Cuts at: 2880 3638 2880 Size: 758 5555 MfeI C'AATT_G Cuts at: 3912 3912 Size: 6313 MluI A'CGCG_T Cuts at: 6189 6189 Size: 6313 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2457 3321 5427 5611 1305 Size: 1152 864 2106 184 2007 Fragments arranged by size: 2106 2007 1152 864 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2780 2891 10 Size: 54 1425 343 948 111 3432 Fragments arranged by size: 3432 1425 948 343 111 54 MslI CAynn'nnrTG Cuts at: 518 1166 2270 2715 2741 2885 3191 4271 4630 Size: 648 1104 445 26 144 306 1080 359 Cuts at: 4630 4789 518 Size: 159 2042 Fragments arranged by size: 2042 1104 1080 648 445 359 306 159 144 26 MspA1I CmG'CkG Cuts at: 1606 2044 2148 2174 2248 2640 2689 3100 3729 Size: 438 104 26 74 392 49 411 629 Cuts at: 3729 4406 5554 5799 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 629 438 411 392 245 104 74 49 26 NarI GG'CG_CC Cuts at: 2047 2426 2047 Size: 379 5934 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4822 Fragments arranged by size: 4822 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6313 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6313 NotI GC'GGCC_GC Cuts at: 2175 3760 2175 Size: 1585 4728 NspI r_CATG'y Cuts at: 1132 1229 3671 5216 1132 Size: 97 2442 1545 2229 Fragments arranged by size: 2442 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3404 1348 Size: 2056 4257 PciI A'CATG_T Cuts at: 5212 5212 Size: 6313 PflMI CCAn_nnn'nTGG Cuts at: 1108 3294 1108 Size: 2186 4127 PfoI T'CCnGG_A Cuts at: 3558 3558 Size: 6313 PmeI GTTT'AAAC Cuts at: 3617 3617 Size: 6313 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4382 4414 5895 5927 4382 Size: 32 1481 32 4768 Fragments arranged by size: 4768 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2523 2865 3169 2523 Size: 342 304 5667 Fragments arranged by size: 5667 342 304 PshAI GACnn'nnGTC Cuts at: 1613 2482 1613 Size: 869 5444 PsiI TTA'TAA Cuts at: 3883 3883 Size: 6313 PspOMI G'GGCC_C Cuts at: 2242 2242 Size: 6313 PstI C_TGCA'G Cuts at: 830 2685 2940 3552 3665 830 Size: 1855 255 612 113 3478 Fragments arranged by size: 3478 1855 612 255 113 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6313 PvuII CAG'CTG Cuts at: 2689 3100 2689 Size: 411 5902 SacI G_AGCT'C Cuts at: 3378 3378 Size: 6313 SacII CC_GC'GG Cuts at: 2149 2175 2149 Size: 26 6287 SalI G'TCGA_C Cuts at: 1936 3655 1936 Size: 1719 4594 SbfI CC_TGCA'GG Cuts at: 2940 3552 3665 2940 Size: 612 113 5588 Fragments arranged by size: 5588 612 113 ScaI AGT'ACT Cuts at: 4561 6273 4561 Size: 1712 4601 SexAI A'CCwGG_T Cuts at: 3204 3432 3204 Size: 228 6085 SfcI C'TryA_G Cuts at: 826 1045 2681 2936 3548 3661 4796 5477 5668 Size: 219 1636 255 612 113 1135 681 191 Cuts at: 5668 826 Size: 1471 Fragments arranged by size: 1636 1471 1135 681 612 255 219 191 113 SfiI GGCCn_nnn'nGGCC Cuts at: 2148 2148 Size: 6313 SfoI GGC'GCC Cuts at: 2048 2427 2048 Size: 379 5934 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6313 SmaI CCC'GGG Cuts at: 1872 3601 3640 1872 Size: 1729 39 4545 Fragments arranged by size: 4545 1729 39 SmlI C'TyrA_G Cuts at: 820 1017 1943 3062 4417 5318 5580 820 Size: 197 926 1119 1355 901 262 1553 Fragments arranged by size: 1553 1355 1119 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4104 493 Size: 3611 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6313 SphI G_CATG'C Cuts at: 1132 3671 1132 Size: 2539 3774 SspI AAT'ATT Cuts at: 5 52 4237 5 Size: 47 4185 2081 Fragments arranged by size: 4185 2081 47 StuI AGG'CCT Cuts at: 1517 2930 3554 1517 Size: 1413 624 4276 Fragments arranged by size: 4276 1413 624 StyI C'CwwG_G Cuts at: 513 1065 2004 2028 2595 2781 3752 513 Size: 552 939 24 567 186 971 3074 Fragments arranged by size: 3074 971 939 567 552 186 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2681 4340 4357 4510 4695 5140 325 Size: 1255 1101 1659 17 153 185 445 1498 Fragments arranged by size: 1659 1498 1255 1101 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3030 3258 4559 Size: 274 80 33 51 1443 1053 228 1301 Cuts at: 4559 6271 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1301 1053 274 228 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2905 2937 6273 6305 2905 Size: 32 3336 32 2913 Fragments arranged by size: 3336 2913 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2219 1316 Size: 903 5410 XbaI T'CTAG_A Cuts at: 3649 3649 Size: 6313 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2419 2843 3190 2419 Size: 424 347 5542 Fragments arranged by size: 5542 424 347 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6313 XmaI C'CCGG_G Cuts at: 1870 3599 3638 1870 Size: 1729 39 4545 Fragments arranged by size: 4545 1729 39 XmnI GAAnn'nnTTC Cuts at: 4442 4442 Size: 6313 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4121 276 Size: 53 83 186 936 2587 2468 Fragments arranged by size: 2587 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsmBI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI SrfI SwaI