(Circular) (Six-base) MAPSORT of: pFN21AE1077.seq Check: 631 from: 1 to: 5389 pFN21AE1077 5389 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3199 278 Size: 53 83 186 936 1663 2468 Fragments arranged by size: 2468 1663 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2732 1937 Size: 795 4594 Acc65I G'GTAC_C Cuts at: 2710 2710 Size: 5389 AclI AA'CG_TT Cuts at: 1563 3516 3889 1563 Size: 1953 373 3063 Fragments arranged by size: 3063 1953 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2195 2378 2560 3452 4836 5104 1632 Size: 563 183 182 892 1384 268 1917 Fragments arranged by size: 1917 1384 892 563 268 183 182 AfeI AGC'GCT Cuts at: 2212 2672 2212 Size: 460 4929 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5192 AflIII A'CryG_T Cuts at: 1203 2087 2140 4288 5265 1203 Size: 884 53 2148 977 1327 Fragments arranged by size: 2148 1327 977 884 53 AgeI A'CCGG_T Cuts at: 2601 4220 2601 Size: 1619 3770 AhdI GACnn_n'nnGTC Cuts at: 4118 4118 Size: 5389 AleI CACnn'nnGTG Cuts at: 1166 2621 1166 Size: 1455 3934 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5389 AlwNI CAG_nnn'CTG Cuts at: 3132 4704 3132 Size: 1572 3817 ApaLI G'TGCA_C Cuts at: 3446 4602 3446 Size: 1156 4233 ApoI r'AATT_y Cuts at: 1493 2293 2698 2924 3076 5185 1493 Size: 800 405 226 152 2109 1697 Fragments arranged by size: 2109 1697 800 405 226 152 AseI AT'TA_AT Cuts at: 160 1032 3943 160 Size: 872 2911 1606 Fragments arranged by size: 2911 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5389 AvaI C'yCGr_G Cuts at: 1870 1943 2243 2714 1870 Size: 73 300 471 4545 Fragments arranged by size: 4545 471 300 73 AvrII C'CTAG_G Cuts at: 2074 2074 Size: 5389 BamHI G'GATC_C Cuts at: 2719 3086 2719 Size: 367 5022 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2128 2218 2598 2710 4165 Size: 325 217 627 341 90 380 112 1455 Cuts at: 4165 618 Size: 1842 Fragments arranged by size: 1842 1455 627 380 341 325 217 112 90 BanII G_rGCy'C Cuts at: 1391 2244 2708 1391 Size: 853 464 4072 Fragments arranged by size: 4072 853 464 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4418 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3580 3614 1903 Size: 34 1643 34 3678 Fragments arranged by size: 3678 1643 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2497 3281 4491 2497 Size: 784 1210 3395 Fragments arranged by size: 3395 1210 784 BclI T'GATC_A Cuts at: 1548 2458 1548 Size: 910 4479 BfrBI ATG'CAT Cuts at: 2387 2387 Size: 5389 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4000 136 Size: 107 122 71 631 605 337 1991 1525 Fragments arranged by size: 1991 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5384 5384 Size: 5389 BlpI GC'TnA_GC Cuts at: 2806 2806 Size: 5389 Bme1580I G_kGCm'C Cuts at: 1790 2133 3450 4606 1790 Size: 343 1317 1156 2573 Fragments arranged by size: 2573 1317 1156 343 BmgBI CAC'GTC Cuts at: 1318 2143 1318 Size: 825 4564 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3120 4078 442 Size: 416 53 83 399 24 1703 958 1753 Fragments arranged by size: 1753 1703 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5389 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4049 1239 Size: 2810 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3514 4379 4677 3514 Size: 865 298 4226 Fragments arranged by size: 4226 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2544 4052 882 Size: 617 1045 1508 2219 Fragments arranged by size: 2219 1508 1045 617 BsaAI yAC'GTr Cuts at: 493 1204 2088 2323 3180 493 Size: 711 884 235 857 2702 Fragments arranged by size: 2702 884 857 711 235 BsaBI GATnn'nnATC Cuts at: 5383 5383 Size: 5389 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2226 3196 3578 4213 Size: 53 83 186 936 693 970 382 635 Cuts at: 4213 275 Size: 1451 Fragments arranged by size: 1451 970 936 693 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2313 2601 3822 4220 4494 4641 5052 2313 Size: 288 1221 398 274 147 411 2650 Fragments arranged by size: 2650 1221 411 398 288 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5164 Fragments arranged by size: 5164 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5389 BseYI C'CCAG_C Cuts at: 4592 5088 4592 Size: 496 4893 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4816 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2839 3600 4628 1540 Size: 275 186 838 761 1028 2301 Fragments arranged by size: 2301 1028 838 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2244 2264 2586 3450 3535 4606 2244 Size: 20 322 864 85 1071 3027 Fragments arranged by size: 3027 1071 864 322 85 20 BsmI GAATG_Cn' Cuts at: 2900 2993 2900 Size: 93 5296 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 2244 2264 2586 2708 3450 3535 Size: 399 343 111 20 322 122 742 85 Cuts at: 3535 4606 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 343 322 122 111 85 20 BspEI T'CCGG_A Cuts at: 5052 5052 Size: 5389 BspHI T'CATG_A Cuts at: 3278 5008 3278 Size: 1730 3659 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2744 844 Size: 1900 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2248 3276 1394 Size: 429 425 1028 3507 Fragments arranged by size: 3507 1028 429 425 BsrDI GCAATG_nn' Cuts at: 65 1248 3884 4058 65 Size: 1183 2636 174 1396 Fragments arranged by size: 2636 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2053 2119 2595 2601 4033 4220 1700 Size: 110 243 66 476 6 1432 187 2869 Fragments arranged by size: 2869 1432 476 243 187 110 66 6 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5389 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5389 BssSI C'ACGA_G Cuts at: 3449 4461 3449 Size: 1012 4377 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5389 BstBI TT'CG_AA Cuts at: 4193 4193 Size: 5389 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5389 BstXI CCAn_nnnn'nTGG Cuts at: 2515 2515 Size: 5389 BstYI r'GATC_y Cuts at: 1972 2310 2719 3086 3471 3488 4940 5026 5038 Size: 338 409 367 385 17 1452 86 12 Cuts at: 5038 5384 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 409 385 367 346 338 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2235 2278 513 Size: 552 939 231 43 3624 Fragments arranged by size: 3624 939 552 231 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4650 Fragments arranged by size: 4650 683 56 BtsI GCAGTG_nn' Cuts at: 2479 2905 3699 3719 2479 Size: 426 794 20 4149 Fragments arranged by size: 4149 794 426 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5389 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5354 DraI TTT'AAA Cuts at: 2693 2693 Size: 5389 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4396 809 Size: 507 3080 1802 Fragments arranged by size: 3080 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2836 3725 5354 Size: 54 1425 50 275 18 1006 889 1629 Cuts at: 5354 8 Size: 43 Fragments arranged by size: 1629 1425 1006 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2836 1537 Size: 275 1024 4090 Fragments arranged by size: 4090 1024 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2397 2637 3319 1353 Size: 1044 240 682 3423 Fragments arranged by size: 3423 1044 682 240 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2075 3976 4350 4496 387 Size: 1064 138 486 1901 374 146 1280 Fragments arranged by size: 1901 1280 1064 486 374 146 138 EcoICRI GAG'CTC Cuts at: 2242 2242 Size: 5389 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2195 2378 2560 3452 4049 4836 5104 Size: 393 563 183 182 892 597 787 268 Cuts at: 5104 1239 Size: 1524 Fragments arranged by size: 1524 892 787 597 563 393 268 183 182 EcoRI G'AATT_C Cuts at: 2698 3076 2698 Size: 378 5011 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2314 2314 Size: 5389 FspI TGC'GCA Cuts at: 2040 3895 2040 Size: 1855 3534 HaeII r_GCGC'y Cuts at: 1678 2214 2571 2596 2674 4536 1678 Size: 536 357 25 78 1862 2531 Fragments arranged by size: 2531 1862 536 357 78 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2495 2527 2571 2603 4030 Size: 32 135 32 923 32 44 32 1427 Cuts at: 4030 4062 4104 4136 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1427 923 135 44 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2733 2979 669 Size: 988 281 795 246 3079 Fragments arranged by size: 3079 988 795 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5389 HpaI GTT'AAC Cuts at: 2979 2979 Size: 5389 KpnI G_GTAC'C Cuts at: 2714 2714 Size: 5389 MfeI C'AATT_G Cuts at: 2988 2988 Size: 5389 MluI A'CGCG_T Cuts at: 5265 5265 Size: 5389 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4503 4687 1305 Size: 3198 184 2007 Fragments arranged by size: 3198 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3567 Fragments arranged by size: 3567 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2621 3347 3706 3865 518 Size: 648 1455 726 359 159 2042 Fragments arranged by size: 2042 1455 726 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2020 2044 2805 3482 4630 4875 1606 Size: 414 24 761 677 1148 245 2120 Fragments arranged by size: 2120 1148 761 677 414 245 24 NaeI GCC'GGC Cuts at: 2055 2597 2055 Size: 542 4847 NcoI C'CATG_G Cuts at: 513 1065 2004 2278 513 Size: 552 939 274 3624 Fragments arranged by size: 3624 939 552 274 NdeI CA'TA_TG Cuts at: 387 387 Size: 5389 NgoMIV G'CCGG_C Cuts at: 2053 2595 2053 Size: 542 4847 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5389 NotI GC'GGCC_GC Cuts at: 2836 2836 Size: 5389 NsiI A_TGCA'T Cuts at: 2389 2389 Size: 5389 NspI r_CATG'y Cuts at: 1132 1229 2160 2747 4292 1132 Size: 97 931 587 1545 2229 Fragments arranged by size: 2229 1545 931 587 97 PasI CC'CwG_GG Cuts at: 1348 2544 1348 Size: 1196 4193 PciI A'CATG_T Cuts at: 4288 4288 Size: 5389 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5389 PmeI GTTT'AAAC Cuts at: 2693 2693 Size: 5389 PmlI CAC'GTG Cuts at: 2088 2323 2088 Size: 235 5154 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3458 3490 4971 5003 3458 Size: 32 1481 32 3844 Fragments arranged by size: 3844 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5389 PsiI TTA'TAA Cuts at: 2959 2959 Size: 5389 PspXI vC'TCGA_Gb Cuts at: 2243 2243 Size: 5389 PstI C_TGCA'G Cuts at: 830 2166 2741 830 Size: 1336 575 3478 Fragments arranged by size: 3478 1336 575 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5389 PvuII CAG'CTG Cuts at: 2044 2044 Size: 5389 SacI G_AGCT'C Cuts at: 2244 2244 Size: 5389 SalI G'TCGA_C Cuts at: 1936 2731 1936 Size: 795 4594 SbfI CC_TGCA'GG Cuts at: 2166 2741 2166 Size: 575 4814 ScaI AGT'ACT Cuts at: 3637 5349 3637 Size: 1712 3677 SexAI A'CCwGG_T Cuts at: 2685 2685 Size: 5389 SfcI C'TryA_G Cuts at: 826 1045 2162 2737 3872 4553 4744 826 Size: 219 1117 575 1135 681 191 1471 Fragments arranged by size: 1471 1135 1117 681 575 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5389 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5389 SmaI CCC'GGG Cuts at: 1872 2716 1872 Size: 844 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2243 3493 4394 4656 820 Size: 197 926 300 1250 901 262 1553 Fragments arranged by size: 1553 1250 926 901 300 262 197 SnaBI TAC'GTA Cuts at: 493 3180 493 Size: 2687 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5389 SphI G_CATG'C Cuts at: 1132 2160 2747 1132 Size: 1028 587 3774 Fragments arranged by size: 3774 1028 587 SspI AAT'ATT Cuts at: 5 52 3313 5 Size: 47 3261 2081 Fragments arranged by size: 3261 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5389 StyI C'CwwG_G Cuts at: 513 1065 2004 2074 2278 2399 2828 513 Size: 552 939 70 204 121 429 3074 Fragments arranged by size: 3074 939 552 429 204 121 70 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3416 3433 3586 3771 4216 325 Size: 1255 1836 17 153 185 445 1498 Fragments arranged by size: 1836 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3635 5347 96 Size: 274 80 33 51 1443 1658 1712 138 Fragments arranged by size: 1712 1658 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2211 2243 5349 5381 2211 Size: 32 3106 32 2219 Fragments arranged by size: 3106 2219 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5389 XbaI T'CTAG_A Cuts at: 2725 2725 Size: 5389 XhoI C'TCGA_G Cuts at: 1943 2243 1943 Size: 300 5089 XmaI C'CCGG_G Cuts at: 1870 2714 1870 Size: 844 4545 XmnI GAAnn'nnTTC Cuts at: 2297 3518 2297 Size: 1221 4168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3197 276 Size: 53 83 186 936 1663 2468 Fragments arranged by size: 2468 1663 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI PacI PfoI PpuMI PspOMI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI