(Circular) (Six-base) MAPSORT of: pFN21AB9268.seq Check: 8219 from: 1 to: 6433 pFN21AB9268 6433 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4243 278 Size: 53 83 186 936 2707 2468 Fragments arranged by size: 2707 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3776 1937 Size: 1839 4594 Acc65I G'GTAC_C Cuts at: 3754 3754 Size: 6433 AclI AA'CG_TT Cuts at: 1563 4560 4933 1563 Size: 2997 373 3063 Fragments arranged by size: 3063 2997 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2237 2406 2774 4496 5880 6148 1632 Size: 605 169 368 1722 1384 268 1917 Fragments arranged by size: 1917 1722 1384 605 368 268 169 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6236 AflIII A'CryG_T Cuts at: 1203 2086 5332 6309 1203 Size: 883 3246 977 1327 Fragments arranged by size: 3246 1327 977 883 AgeI A'CCGG_T Cuts at: 5264 5264 Size: 6433 AhdI GACnn_n'nnGTC Cuts at: 3116 5162 3116 Size: 2046 4387 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6433 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6433 AlwNI CAG_nnn'CTG Cuts at: 2932 4176 5748 2932 Size: 1244 1572 3617 Fragments arranged by size: 3617 1572 1244 ApaLI G'TGCA_C Cuts at: 4490 5646 4490 Size: 1156 5277 ApoI r'AATT_y Cuts at: 1493 3437 3742 3968 4120 6229 1493 Size: 1944 305 226 152 2109 1697 Fragments arranged by size: 2109 1944 1697 305 226 152 AseI AT'TA_AT Cuts at: 160 1032 4987 160 Size: 872 3955 1606 Fragments arranged by size: 3955 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6433 AvaI C'yCGr_G Cuts at: 1870 1943 3758 1870 Size: 73 1815 4545 Fragments arranged by size: 4545 1815 73 BamHI G'GATC_C Cuts at: 3763 4130 3763 Size: 367 6066 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2200 3709 3754 5209 618 Size: 325 217 627 413 1509 45 1455 1842 Fragments arranged by size: 1842 1509 1455 627 413 325 217 45 BanII G_rGCy'C Cuts at: 1391 3669 3752 1391 Size: 2278 83 4072 Fragments arranged by size: 4072 2278 83 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2109 2412 928 Size: 971 210 303 4949 Fragments arranged by size: 4949 971 303 210 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2825 2859 4624 4658 1903 Size: 34 50 34 804 34 1765 34 3678 Fragments arranged by size: 3678 1765 804 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2568 4325 5535 2568 Size: 1757 1210 3466 Fragments arranged by size: 3466 1757 1210 BclI T'GATC_A Cuts at: 1548 3034 3171 1548 Size: 1486 137 4810 Fragments arranged by size: 4810 1486 137 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5044 136 Size: 107 122 71 631 605 3372 1525 Fragments arranged by size: 3372 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6428 6428 Size: 6433 BlpI GC'TnA_GC Cuts at: 3850 3850 Size: 6433 Bme1580I G_kGCm'C Cuts at: 1790 2205 3207 4494 5650 1790 Size: 415 1002 1287 1156 2573 Fragments arranged by size: 2573 1287 1156 1002 415 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6433 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2360 2459 4164 Size: 416 53 83 399 24 943 99 1705 Cuts at: 4164 5122 442 Size: 958 1753 Fragments arranged by size: 1753 1705 958 943 416 399 99 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6433 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2425 2425 Size: 6433 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5093 1239 Size: 3854 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4558 5423 5721 4558 Size: 865 298 5270 Fragments arranged by size: 5270 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3692 5096 882 Size: 617 2193 1404 2219 Fragments arranged by size: 2219 2193 1404 617 BsaAI yAC'GTr Cuts at: 493 1204 4224 493 Size: 711 3020 2702 Fragments arranged by size: 3020 2702 711 BsaBI GATnn'nnATC Cuts at: 2699 6427 2699 Size: 3728 2705 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4240 4622 5257 275 Size: 53 83 186 936 2707 382 635 1451 Fragments arranged by size: 2707 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2197 4866 5264 5538 5685 6096 2197 Size: 2669 398 274 147 411 2534 Fragments arranged by size: 2669 2534 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6208 Fragments arranged by size: 6208 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2526 2529 2684 1186 Size: 1340 3 155 4935 Fragments arranged by size: 4935 1340 155 3 BseYI C'CCAG_C Cuts at: 2073 2759 3183 5636 6132 2073 Size: 686 424 2453 496 2374 Fragments arranged by size: 2453 2374 686 496 424 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3570 1163 Size: 573 1834 4026 Fragments arranged by size: 4026 1834 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3883 4644 5672 1540 Size: 275 186 1882 761 1028 2301 Fragments arranged by size: 2301 1882 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3669 4494 4579 5650 3669 Size: 825 85 1071 4452 Fragments arranged by size: 4452 1071 825 85 BsmI GAATG_Cn' Cuts at: 2146 3026 3656 3944 4037 2146 Size: 880 630 288 93 4542 Fragments arranged by size: 4542 880 630 288 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2899 2899 Size: 6433 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2205 3207 3669 3752 4494 4579 5650 Size: 399 415 1002 462 83 742 85 1071 Cuts at: 5650 1391 Size: 2174 Fragments arranged by size: 2174 1071 1002 742 462 415 399 85 83 BspEI T'CCGG_A Cuts at: 6096 6096 Size: 6433 BspHI T'CATG_A Cuts at: 2164 2505 4322 6052 2164 Size: 341 1817 1730 2545 Fragments arranged by size: 2545 1817 1730 341 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3788 844 Size: 2944 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4320 1394 Size: 429 2497 3507 Fragments arranged by size: 3507 2497 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4928 5102 65 Size: 1183 3680 174 1396 Fragments arranged by size: 3680 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5077 5264 1700 Size: 110 3267 187 2869 Fragments arranged by size: 3267 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2332 3289 96 Size: 2236 957 3240 Fragments arranged by size: 3240 2236 957 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6433 BssSI C'ACGA_G Cuts at: 4493 5505 4493 Size: 1012 5421 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6433 BstBI TT'CG_AA Cuts at: 5237 5237 Size: 6433 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6433 BstXI CCAn_nnnn'nTGG Cuts at: 3191 3720 3191 Size: 529 5904 BstYI r'GATC_y Cuts at: 1972 2024 3763 4130 4515 4532 5984 6070 6082 Size: 52 1739 367 385 17 1452 86 12 Cuts at: 6082 6428 1972 Size: 346 1977 Fragments arranged by size: 1977 1739 1452 385 367 346 86 52 17 12 Bsu36I CC'TnA_GG Cuts at: 2598 2598 Size: 6433 BtgI C'CryG_G Cuts at: 513 1065 2212 3132 513 Size: 552 1147 920 3814 Fragments arranged by size: 3814 1147 920 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5694 Fragments arranged by size: 5694 683 56 BtsI GCAGTG_nn' Cuts at: 3949 4743 4763 3949 Size: 794 20 5619 Fragments arranged by size: 5619 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6433 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6398 DraI TTT'AAA Cuts at: 3737 3737 Size: 6433 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5440 809 Size: 507 4124 1802 Fragments arranged by size: 4124 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2229 2241 2496 Size: 54 1425 50 275 18 399 12 255 Cuts at: 2496 3880 4769 6398 8 Size: 1384 889 1629 43 Fragments arranged by size: 1629 1425 1384 889 399 275 255 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 3880 1537 Size: 275 2068 4090 Fragments arranged by size: 4090 2068 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2241 3023 4363 1353 Size: 888 782 1340 3423 Fragments arranged by size: 3423 1340 888 782 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5020 5394 5540 387 Size: 1064 138 3431 374 146 1280 Fragments arranged by size: 3431 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3667 3667 Size: 6433 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 2406 2774 4496 5093 5880 6148 Size: 393 605 169 368 1722 597 787 268 Cuts at: 6148 1239 Size: 1524 Fragments arranged by size: 1722 1524 787 605 597 393 368 268 169 EcoRI G'AATT_C Cuts at: 3742 4120 3742 Size: 378 6055 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2780 2780 Size: 6433 FspI TGC'GCA Cuts at: 4939 4939 Size: 6433 HaeII r_GCGC'y Cuts at: 1678 5580 1678 Size: 3902 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2605 2637 2663 2695 3067 Size: 32 135 32 1033 32 26 32 372 Cuts at: 3067 3099 3102 3134 5074 5106 5148 5180 1373 Size: 32 3 32 1940 32 42 32 2626 Fragments arranged by size: 2626 1940 1033 372 135 42 32 32 32 32 32 32 32 32 26 3 HincII GTy'rAC Cuts at: 669 1657 1938 2019 2569 3777 4023 669 Size: 988 281 81 550 1208 246 3079 Fragments arranged by size: 3079 1208 988 550 281 246 81 HindIII A'AGCT_T Cuts at: 748 748 Size: 6433 HpaI GTT'AAC Cuts at: 4023 4023 Size: 6433 KpnI G_GTAC'C Cuts at: 3758 3758 Size: 6433 MfeI C'AATT_G Cuts at: 4032 4032 Size: 6433 MluI A'CGCG_T Cuts at: 6309 6309 Size: 6433 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2967 3375 5547 5731 1305 Size: 1662 408 2172 184 2007 Fragments arranged by size: 2172 2007 1662 408 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2498 10 Size: 54 1425 343 666 3945 Fragments arranged by size: 3945 1425 666 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2181 2547 4391 4750 4909 518 Size: 648 1015 366 1844 359 159 2042 Fragments arranged by size: 2042 1844 1015 648 366 359 159 MspA1I CmG'CkG Cuts at: 1606 2077 3050 3849 4526 5674 5919 1606 Size: 471 973 799 677 1148 245 2120 Fragments arranged by size: 2120 1148 973 799 677 471 245 NcoI C'CATG_G Cuts at: 513 1065 2212 513 Size: 552 1147 4734 Fragments arranged by size: 4734 1147 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6433 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6433 NotI GC'GGCC_GC Cuts at: 3880 3880 Size: 6433 NspI r_CATG'y Cuts at: 1132 1229 2090 3791 5336 1132 Size: 97 861 1701 1545 2229 Fragments arranged by size: 2229 1701 1545 861 97 PasI CC'CwG_GG Cuts at: 1348 2853 1348 Size: 1505 4928 PciI A'CATG_T Cuts at: 2086 5332 2086 Size: 3246 3187 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6433 PfoI T'CCnGG_A Cuts at: 2883 2883 Size: 6433 PmeI GTTT'AAAC Cuts at: 3737 3737 Size: 6433 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4502 4534 6015 6047 4502 Size: 32 1481 32 4888 Fragments arranged by size: 4888 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2040 1613 Size: 427 6006 PsiI TTA'TAA Cuts at: 3461 3632 4003 3461 Size: 171 371 5891 Fragments arranged by size: 5891 371 171 PstI C_TGCA'G Cuts at: 830 2082 2524 3785 830 Size: 1252 442 1261 3478 Fragments arranged by size: 3478 1261 1252 442 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6433 PvuII CAG'CTG Cuts at: 2077 3050 2077 Size: 973 5460 SacI G_AGCT'C Cuts at: 3669 3669 Size: 6433 SalI G'TCGA_C Cuts at: 1936 3775 1936 Size: 1839 4594 SapI GCTCTTCn'nnn_ Cuts at: 2241 2241 Size: 6433 SbfI CC_TGCA'GG Cuts at: 3785 3785 Size: 6433 ScaI AGT'ACT Cuts at: 4681 6393 4681 Size: 1712 4721 SfcI C'TryA_G Cuts at: 826 1045 2078 2520 3781 4916 5597 5788 826 Size: 219 1033 442 1261 1135 681 191 1471 Fragments arranged by size: 1471 1261 1135 1033 681 442 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6433 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6433 SmaI CCC'GGG Cuts at: 1872 3760 1872 Size: 1888 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4537 5438 5700 820 Size: 197 926 2594 901 262 1553 Fragments arranged by size: 2594 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4224 493 Size: 3731 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6433 SphI G_CATG'C Cuts at: 1132 3791 1132 Size: 2659 3774 SspI AAT'ATT Cuts at: 5 52 2919 4357 5 Size: 47 2867 1438 2081 Fragments arranged by size: 2867 2081 1438 47 StuI AGG'CCT Cuts at: 1517 2596 1517 Size: 1079 5354 StyI C'CwwG_G Cuts at: 513 1065 2212 2226 3532 3872 513 Size: 552 1147 14 1306 340 3074 Fragments arranged by size: 3074 1306 1147 552 340 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3197 3351 3697 4460 4477 4630 4815 Size: 1255 1617 154 346 763 17 153 185 Cuts at: 4815 5260 325 Size: 445 1498 Fragments arranged by size: 1617 1498 1255 763 445 346 185 154 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2332 3289 4679 Size: 274 80 33 51 1443 355 957 1390 Cuts at: 4679 6391 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1390 957 355 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6393 6425 6393 Size: 32 6401 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6433 XbaI T'CTAG_A Cuts at: 3769 3769 Size: 6433 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3239 3239 Size: 6433 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6433 XmaI C'CCGG_G Cuts at: 1870 3758 1870 Size: 1888 4545 XmnI GAAnn'nnTTC Cuts at: 2250 3564 4562 2250 Size: 1314 998 4121 Fragments arranged by size: 4121 1314 998 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4241 276 Size: 53 83 186 936 2707 2468 Fragments arranged by size: 2707 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI