(Circular) (Six-base) MAPSORT of: pFN21AE1017.seq Check: 3160 from: 1 to: 6124 pFN21AE1017 6124 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3934 278 Size: 53 83 186 936 2398 2468 Fragments arranged by size: 2468 2398 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3467 1937 Size: 1530 4594 Acc65I G'GTAC_C Cuts at: 3445 3445 Size: 6124 AclI AA'CG_TT Cuts at: 1563 4251 4624 1563 Size: 2688 373 3063 Fragments arranged by size: 3063 2688 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2418 2976 2979 3205 3290 4187 5571 5839 Size: 786 558 3 226 85 897 1384 268 Cuts at: 5839 1632 Size: 1917 Fragments arranged by size: 1917 1384 897 786 558 268 226 85 3 AfeI AGC'GCT Cuts at: 3260 3260 Size: 6124 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5927 AflIII A'CryG_T Cuts at: 1203 5023 6000 1203 Size: 3820 977 1327 Fragments arranged by size: 3820 1327 977 AgeI A'CCGG_T Cuts at: 4955 4955 Size: 6124 AhdI GACnn_n'nnGTC Cuts at: 2683 4853 2683 Size: 2170 3954 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6124 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6124 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2963 2995 2963 Size: 32 6092 AlwNI CAG_nnn'CTG Cuts at: 3867 5439 3867 Size: 1572 4552 ApaLI G'TGCA_C Cuts at: 2805 4181 5337 2805 Size: 1376 1156 3592 Fragments arranged by size: 3592 1376 1156 ApoI r'AATT_y Cuts at: 1493 2412 2445 2628 3433 3659 3811 5920 1493 Size: 919 33 183 805 226 152 2109 1697 Fragments arranged by size: 2109 1697 919 805 226 183 152 33 AseI AT'TA_AT Cuts at: 160 1032 4678 160 Size: 872 3646 1606 Fragments arranged by size: 3646 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 AvaI C'yCGr_G Cuts at: 1870 1943 3449 1870 Size: 73 1506 4545 Fragments arranged by size: 4545 1506 73 BamHI G'GATC_C Cuts at: 3454 3821 3454 Size: 367 5757 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3445 4900 618 Size: 325 217 627 1658 1455 1842 Fragments arranged by size: 1842 1658 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2188 2588 3313 3443 1391 Size: 797 400 725 130 4072 Fragments arranged by size: 4072 797 725 400 130 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3302 928 Size: 971 1403 3750 Fragments arranged by size: 3750 1403 971 BbvCI CC'TCA_GC Cuts at: 2709 2709 Size: 6124 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4315 4349 1903 Size: 34 2378 34 3678 Fragments arranged by size: 3678 2378 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4016 5226 4016 Size: 1210 4914 BclI T'GATC_A Cuts at: 1548 1548 Size: 6124 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2995 4735 136 Size: 107 122 71 631 605 1323 1740 1525 Fragments arranged by size: 1740 1525 1323 631 605 122 107 71 BglII A'GATC_T Cuts at: 6119 6119 Size: 6124 BlpI GC'TnA_GC Cuts at: 3541 3541 Size: 6124 Bme1580I G_kGCm'C Cuts at: 1790 2809 4185 5341 1790 Size: 1019 1376 1156 2573 Fragments arranged by size: 2573 1376 1156 1019 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6124 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2534 2621 3855 Size: 416 53 83 399 24 1117 87 1234 Cuts at: 3855 4813 442 Size: 958 1753 Fragments arranged by size: 1753 1234 1117 958 416 399 87 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6124 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2288 2934 2967 3327 4784 1239 Size: 1049 646 33 360 1457 2579 Fragments arranged by size: 2579 1457 1049 646 360 33 Bpu10I CC'TnA_GC Cuts at: 2709 2709 Size: 6124 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2834 4249 5114 5412 2834 Size: 1415 865 298 3546 Fragments arranged by size: 3546 1415 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4787 882 Size: 617 3288 2219 Fragments arranged by size: 3288 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3915 493 Size: 711 2711 2702 Fragments arranged by size: 2711 2702 711 BsaBI GATnn'nnATC Cuts at: 6118 6118 Size: 6124 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2323 3931 4313 4948 Size: 53 83 186 936 790 1608 382 635 Cuts at: 4948 275 Size: 1451 Fragments arranged by size: 1608 1451 936 790 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4557 4955 5229 5376 5787 4557 Size: 398 274 147 411 4894 Fragments arranged by size: 4894 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2761 2791 2963 2993 1178 Size: 30 165 30 1358 30 172 30 4309 Fragments arranged by size: 4309 1358 172 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2663 2666 2669 2697 2900 2931 1186 Size: 1477 3 3 28 203 31 4379 Fragments arranged by size: 4379 1477 203 31 28 3 3 BseYI C'CCAG_C Cuts at: 2056 3396 5327 5823 2056 Size: 1340 1931 496 2357 Fragments arranged by size: 2357 1931 1340 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2186 2817 1163 Size: 573 450 631 4470 Fragments arranged by size: 4470 631 573 450 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3574 4335 5363 1540 Size: 275 186 1573 761 1028 2301 Fragments arranged by size: 2301 1573 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2408 2809 3291 4185 4270 5341 2408 Size: 401 482 894 85 1071 3191 Fragments arranged by size: 3191 1071 894 482 401 85 BsmI GAATG_Cn' Cuts at: 2514 3635 3728 2514 Size: 1121 93 4910 Fragments arranged by size: 4910 1121 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2261 2330 2261 Size: 69 6055 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2188 2408 2588 2809 3291 3313 3443 Size: 399 398 220 180 221 482 22 130 Cuts at: 3443 4185 4270 5341 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 482 399 398 221 220 180 130 85 22 BspEI T'CCGG_A Cuts at: 5787 5787 Size: 6124 BspHI T'CATG_A Cuts at: 4013 5743 4013 Size: 1730 4394 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2057 2380 3479 844 Size: 1213 323 1099 3489 Fragments arranged by size: 3489 1213 1099 323 BsrBI CCG'CTC Cuts at: 1394 1823 4011 1394 Size: 429 2188 3507 Fragments arranged by size: 3507 2188 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4619 4793 65 Size: 1183 3371 174 1396 Fragments arranged by size: 3371 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3201 4768 4955 1700 Size: 110 1391 1567 187 2869 Fragments arranged by size: 2869 1567 1391 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6124 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6124 BssSI C'ACGA_G Cuts at: 4184 5196 4184 Size: 1012 5112 BstAPI GCAn_nnn'nTGC Cuts at: 794 3211 794 Size: 2417 3707 BstBI TT'CG_AA Cuts at: 4928 4928 Size: 6124 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6124 BstYI r'GATC_y Cuts at: 1972 3043 3454 3821 4206 4223 5675 5761 5773 Size: 1071 411 367 385 17 1452 86 12 Cuts at: 5773 6119 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1071 411 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2880 2880 Size: 6124 BtgI C'CryG_G Cuts at: 513 1065 2004 2063 2455 513 Size: 552 939 59 392 4182 Fragments arranged by size: 4182 939 552 392 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5385 Fragments arranged by size: 5385 683 56 BtsI GCAGTG_nn' Cuts at: 2202 2759 2834 3189 3402 3640 4434 4454 2202 Size: 557 75 355 213 238 794 20 3872 Fragments arranged by size: 3872 794 557 355 238 213 75 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6124 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6089 DraI TTT'AAA Cuts at: 3428 3428 Size: 6124 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5131 809 Size: 507 3815 1802 Fragments arranged by size: 3815 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2367 3334 3571 Size: 54 1425 50 275 18 537 967 237 Cuts at: 3571 4460 6089 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 967 889 537 275 237 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3571 1537 Size: 275 1759 4090 Fragments arranged by size: 4090 1759 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2352 2527 2640 4054 1353 Size: 999 175 113 1414 3423 Fragments arranged by size: 3423 1414 999 175 113 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2318 3356 4711 5085 5231 387 Size: 1064 138 729 1038 1355 374 146 1280 Fragments arranged by size: 1355 1280 1064 1038 729 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2288 2418 2934 2967 2976 2979 3205 Size: 393 656 130 516 33 9 3 226 Cuts at: 3205 3290 3327 4187 4784 5571 5839 1239 Size: 85 37 860 597 787 268 1524 Fragments arranged by size: 1524 860 787 656 597 516 393 268 226 130 85 37 33 9 3 EcoNI CCTnn'n_nnAGG Cuts at: 2165 2592 3383 2165 Size: 427 791 4906 Fragments arranged by size: 4906 791 427 EcoO109I rG'GnC_Cy Cuts at: 3080 3376 3080 Size: 296 5828 EcoRI G'AATT_C Cuts at: 3433 3811 3433 Size: 378 5746 EcoRV GAT'ATC Cuts at: 2892 2892 Size: 6124 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2589 2589 Size: 6124 FspI TGC'GCA Cuts at: 4630 4630 Size: 6124 HaeII r_GCGC'y Cuts at: 1678 3262 5271 1678 Size: 1584 2009 2531 Fragments arranged by size: 2531 2009 1584 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2390 2422 2669 2701 2761 Size: 32 135 32 818 32 247 32 60 Cuts at: 2761 2793 2876 2908 4765 4797 4839 4871 1373 Size: 32 83 32 1857 32 42 32 2626 Fragments arranged by size: 2626 1857 818 247 135 83 60 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3468 3714 669 Size: 988 281 1530 246 3079 Fragments arranged by size: 3079 1530 988 281 246 HindIII A'AGCT_T Cuts at: 748 2786 748 Size: 2038 4086 HpaI GTT'AAC Cuts at: 3714 3714 Size: 6124 KpnI G_GTAC'C Cuts at: 3449 3449 Size: 6124 MfeI C'AATT_G Cuts at: 3723 3723 Size: 6124 MluI A'CGCG_T Cuts at: 6000 6000 Size: 6124 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5238 5422 1305 Size: 3933 184 2007 Fragments arranged by size: 3933 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2369 10 Size: 54 1425 343 537 3765 Fragments arranged by size: 3765 1425 537 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3054 4082 4441 4600 518 Size: 648 1888 1028 359 159 2042 Fragments arranged by size: 2042 1888 1028 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3540 4217 5365 5610 1606 Size: 1934 677 1148 245 2120 Fragments arranged by size: 2120 1934 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4633 Fragments arranged by size: 4633 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6124 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6124 NotI GC'GGCC_GC Cuts at: 3571 3571 Size: 6124 NspI r_CATG'y Cuts at: 1132 1229 3320 3482 5027 1132 Size: 97 2091 162 1545 2229 Fragments arranged by size: 2229 2091 1545 162 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6124 PciI A'CATG_T Cuts at: 5023 5023 Size: 6124 PflMI CCAn_nnn'nTGG Cuts at: 1108 3011 1108 Size: 1903 4221 PfoI T'CCnGG_A Cuts at: 3029 3029 Size: 6124 PmeI GTTT'AAAC Cuts at: 3428 3428 Size: 6124 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2126 2158 2963 2995 4193 4225 5706 5738 2126 Size: 32 805 32 1198 32 1481 32 2512 Fragments arranged by size: 2512 1481 1198 805 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3080 3376 3080 Size: 296 5828 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6124 PsiI TTA'TAA Cuts at: 3694 3694 Size: 6124 PstI C_TGCA'G Cuts at: 830 3142 3476 830 Size: 2312 334 3478 Fragments arranged by size: 3478 2312 334 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6124 SalI G'TCGA_C Cuts at: 1936 3466 1936 Size: 1530 4594 SapI GCTCTTCn'nnn_ Cuts at: 2352 2527 2352 Size: 175 5949 SbfI CC_TGCA'GG Cuts at: 3476 3476 Size: 6124 ScaI AGT'ACT Cuts at: 4372 6084 4372 Size: 1712 4412 SfcI C'TryA_G Cuts at: 826 1045 2499 3138 3472 4607 5288 5479 826 Size: 219 1454 639 334 1135 681 191 1471 Fragments arranged by size: 1471 1454 1135 681 639 334 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6124 SmaI CCC'GGG Cuts at: 1872 3451 1872 Size: 1579 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2813 4228 5129 5391 820 Size: 197 926 870 1415 901 262 1553 Fragments arranged by size: 1553 1415 926 901 870 262 197 SnaBI TAC'GTA Cuts at: 493 3915 493 Size: 3422 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6124 SphI G_CATG'C Cuts at: 1132 3320 3482 1132 Size: 2188 162 3774 Fragments arranged by size: 3774 2188 162 SspI AAT'ATT Cuts at: 5 52 4048 5 Size: 47 3996 2081 Fragments arranged by size: 3996 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6124 StyI C'CwwG_G Cuts at: 513 1065 2004 3563 513 Size: 552 939 1559 3074 Fragments arranged by size: 3074 1559 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2772 4151 4168 4321 4506 4951 325 Size: 1255 1192 1379 17 153 185 445 1498 Fragments arranged by size: 1498 1379 1255 1192 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2542 4370 6082 Size: 274 80 33 51 1443 565 1828 1712 Cuts at: 6082 96 Size: 138 Fragments arranged by size: 1828 1712 1443 565 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2055 2087 6084 6116 2055 Size: 32 3997 32 2063 Fragments arranged by size: 3997 2063 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6124 XbaI T'CTAG_A Cuts at: 3460 3460 Size: 6124 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3104 3104 Size: 6124 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6124 XmaI C'CCGG_G Cuts at: 1870 3449 1870 Size: 1579 4545 XmnI GAAnn'nnTTC Cuts at: 4253 4253 Size: 6124 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3932 276 Size: 53 83 186 936 2398 2468 Fragments arranged by size: 2468 2398 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI