Restriction map for
pFN21AE1028
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AhdI Alw44I AocI ApaI
ApaLI AscI Asp718I AspEI AvrII
BamHI BbeI BbrPI BbvCI BfrI
BglI BlnI BsaAI BsaBI BsaHI
BseAI BseRI BsgI BsiEI BsiWI
BspEI BsrBI BsrDI BssHII Bst1107I
BstUI BstZ17I Bsu36I CpoI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRI EcoT22I FauI FseI Hin1I
KasI KpnI KspI MamI MluI
MroI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
PacI PmaCI PmeI PmlI PpuMI
PshAI PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgrAI SmaI
SnaBI Sse8387I SwaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 2313 GTCTAC GT^[AC][GT]AC
AciI 180 GCGG CCGC(-3/-1)
AclI 971 AACGTT AA^CGTT
AflIII 1872 ACATGT A^C[GA][CT]GT
AgeI 260 ACCGGT A^CCGGT
AspHI 1985 GTGCTC G[AT]GC[AT]^C
AspI 1966 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2099 TGCGCA TGC^GCA
BalI 3614 TGGCCA TGG^CCA
BanI 307 GGTGCC G^G[CT][GA]CC
BanII 662 GAGCCC G[GA]GC[CT]^C
BbsI 1261 GAAGAC GAAGAC(2/6)
BcgI 699 CGAGACTCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1788 GGATAC GTATCC(6/5)
BcnI 3405 CCCGG CC^[GC]GG
BglII 1464 AGATCT A^GATCT
BpuAI 1261 GAAGAC GAAGAC(2/6)
BsaI 2164 GAGACC GGTCTC(1/5)
BsaWI 260 ACCGGT [AT]^CCGG[AT]
BsiHKAI 1985 GTGCTC G[AT]GC[AT]^C
BsmBI 657 GAGACG CGTCTC(1/5)
BsmFI 3259 GTCCC GGGAC(10/14)
BsmI 1428 GAATGC GAATGC(1/-1)
BspHI 2748 TCATGA T^CATGA
BspLU11I 1872 ACATGT A^CATGT
BsrFI 260 ACCGGT [GA]^CCGG[CT]
BstXI 3273 CCAGTTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 260 ACCGGT [GA]^CCGG[CT]
EaeI 3614 TGGCCA [CT]^GGCC[GA]
EcoRV 805 GATATC GAT^ATC
FspI 2099 TGCGCA TGC^GCA
HgaI 3006 GCGTC GACGC(5/10)
MfeI 53 CAATTG C^AATTG
MluNI 3614 TGGCCA TGG^CCA
MscI 3614 TGGCCA TGG^CCA
MunI 53 CAATTG C^AATTG
NciI 3405 CCCGG CC^[GC]GG
NdeI 2731 CATATG CA^TATG
PflFI 1966 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3438 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 260 ACCGGT A^CCGGT
Psp1406I 971 AACGTT AA^CGTT
RcaI 2748 TCATGA T^CATGA
SexAI 3144 ACCTGGT A^CC[AT]GGT
SpeI 742 ACTAGT A^CTAGT
StuI 3526 AGGCCT AGG^CCT
Tth111I 1966 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3438 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 40 enzymes) #
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Aor51HI 379 AGCGCT AGC^GCT
2705 AGCGCT
AseI 1216 ATTAAT AT^TAAT
2119 ATTAAT
AsnI 1216 ATTAAT AT^TAAT
2119 ATTAAT
Asp700I 2250 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3079 GAAGCTATTC
AvaI 1337 CTCGAG C^[CT]CG[GA]G
2477 CTCGAG
BmyI 662 GAGCCC G[AGT]GC[ACT]^C
1985 GTGCTC
BsiYI 2168 CCCAGCAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3438 CCAGGCTCTGG
BslI 2168 CCCAGCAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3438 CCAGGCTCTGG
BsoBI 1337 CTCGAG C^[CT]CG[GA]G
2477 CTCGAG
Bsp1286I 662 GAGCCC G[AGT]GC[ACT]^C
1985 GTGCTC
Bsp1407I 2 TGTACA T^GTACA
3495 TGTACA
BspDI 271 ATCGAT AT^CGAT
3036 ATCGAT
BspMI 2096 ACCTGC ACCTGC(4/8)
12 GCAGGT
BsrGI 2 TGTACA T^GTACA
3495 TGTACA
BssSI 212 CTCGTG CACGAG(-5/-1)
704 CTCGTG
BstEII 263 GGTTACC G^GT[ACGT]ACC
1963 GGTGACC
BstPI 263 GGTTACC G^GT[ACGT]ACC
1963 GGTGACC
ClaI 271 ATCGAT AT^CGAT
3036 ATCGAT
DraI 1515 TTTAAA TTT^AAA
2182 TTTAAA
Eco47III 379 AGCGCT AGC^GCT
2705 AGCGCT
EcoO65I 263 GGTTACC G^GT[ACGT]ACC
1963 GGTGACC
EcoT14I 926 CCAAGG C^C[AT][AT]GG
3362 CCTTGG
HaeII 379 AGCGCT [GA]GCGC^[CT]
2705 AGCGCT
HapII 261 CCGG C^CGG
3406 CCGG
HindIII 1862 AAGCTT A^AGCTT
2295 AAGCTT
HpaI 2944 GTTAAC GTT^AAC
3090 GTTAAC
HpaII 261 CCGG C^CGG
3406 CCGG
MaeII 972 ACGT A^CGT
2271 ACGT
MspI 261 CCGG C^CGG
3406 CCGG
NlaIV 307 GGTGCC GG[ACGT]^[ACGT]CC
3408 GGCTCC
PaeR7I 1337 CTCGAG C^TCGAG
2477 CTCGAG
PshBI 1216 ATTAAT AT^TAAT
2119 ATTAAT
PvuII 1921 CAGCTG CAG^CTG
3545 CAGCTG
SphI 2301 GCATGC GCATG^C
2789 GCATGC
SspBI 2 TGTACA T^GTACA
3495 TGTACA
SspI 1361 AATATT AAT^ATT
2107 AATATT
StyI 926 CCAAGG C^C[AT][AT]GG
3362 CCTTGG
XbaI 606 TCTAGA T^CTAGA
2191 TCTAGA
XhoI 1337 CTCGAG C^TCGAG
2477 CTCGAG
XmnI 2250 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3079 GAAGCTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AvaII BbvI BclI BfaI
BlpI BpmI Bpu1102I BsaJI BsmAI
BsrI BssKI BstAPI BstBI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII EarI EcoRII
FbaI Fnu4HI FokI HaeIII HhaI
HinP1I HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NspI NspV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfuI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII