(Circular) (Six-base) MAPSORT of: pFN21AE1028.seq Check: 3099 from: 1 to: 8356 pFN21AE1028 8356 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2008 2008 Size: 8356 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6166 278 Size: 53 83 186 936 4630 2468 Fragments arranged by size: 4630 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4319 5699 1937 Size: 2382 1380 4594 Fragments arranged by size: 4594 2382 1380 Acc65I G'GTAC_C Cuts at: 5677 5677 Size: 8356 AclI AA'CG_TT Cuts at: 1563 2977 6483 6856 1563 Size: 1414 3506 373 3063 Fragments arranged by size: 3506 3063 1414 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2056 3294 4409 4679 5107 6419 7803 8071 Size: 424 1238 1115 270 428 1312 1384 268 Cuts at: 8071 1632 Size: 1917 Fragments arranged by size: 1917 1384 1312 1238 1115 428 424 270 268 AfeI AGC'GCT Cuts at: 2386 4712 2386 Size: 2326 6030 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8159 AflIII A'CryG_T Cuts at: 1203 3877 7255 8232 1203 Size: 2674 3378 977 1327 Fragments arranged by size: 3378 2674 1327 977 AgeI A'CCGG_T Cuts at: 2265 7187 2265 Size: 4922 3434 AhdI GACnn_n'nnGTC Cuts at: 7085 7085 Size: 8356 AleI CACnn'nnGTG Cuts at: 1166 3650 1166 Size: 2484 5872 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3468 1184 Size: 2284 6072 AlwNI CAG_nnn'CTG Cuts at: 3001 4679 4882 5449 6099 7671 3001 Size: 1678 203 567 650 1572 3686 Fragments arranged by size: 3686 1678 1572 650 567 203 ApaLI G'TGCA_C Cuts at: 6413 7569 6413 Size: 1156 7200 ApoI r'AATT_y Cuts at: 1493 2844 3053 4184 4830 4978 5558 5665 5891 Size: 1351 209 1131 646 148 580 107 226 Cuts at: 5891 6043 8152 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1351 1131 646 580 226 209 152 148 107 AseI AT'TA_AT Cuts at: 160 1032 3222 4125 6910 160 Size: 872 2190 903 2785 1606 Fragments arranged by size: 2785 2190 1606 903 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8356 AvaI C'yCGr_G Cuts at: 1870 1943 3342 4482 5681 1870 Size: 73 1399 1140 1199 4545 Fragments arranged by size: 4545 1399 1199 1140 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2487 2520 2487 Size: 33 8323 BamHI G'GATC_C Cuts at: 5686 6053 5686 Size: 367 7989 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2312 5677 7132 618 Size: 325 217 627 525 3365 1455 1842 Fragments arranged by size: 3365 1842 1455 627 525 325 217 BanII G_rGCy'C Cuts at: 1391 2671 5675 1391 Size: 1280 3004 4072 Fragments arranged by size: 4072 3004 1280 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3273 928 Size: 971 1374 6011 Fragments arranged by size: 6011 1374 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2693 2727 6547 6581 1903 Size: 34 756 34 3820 34 3678 Fragments arranged by size: 3820 3678 756 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3787 6248 7458 3787 Size: 2461 1210 4685 Fragments arranged by size: 4685 2461 1210 BclI T'GATC_A Cuts at: 1548 3831 4138 5604 1548 Size: 2283 307 1466 4300 Fragments arranged by size: 4300 2283 1466 307 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6967 136 Size: 107 122 71 631 605 5295 1525 Fragments arranged by size: 5295 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3469 8351 3469 Size: 4882 3474 BlpI GC'TnA_GC Cuts at: 2736 2781 5457 5773 2736 Size: 45 2676 316 5319 Fragments arranged by size: 5319 2676 316 45 Bme1580I G_kGCm'C Cuts at: 1790 6417 7573 1790 Size: 4627 1156 2573 Fragments arranged by size: 4627 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8356 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4008 4355 6087 Size: 416 53 83 399 24 2591 347 1732 Cuts at: 6087 7045 442 Size: 958 1753 Fragments arranged by size: 2591 1753 1732 958 416 399 347 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8356 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3317 3771 5307 7016 1239 Size: 2078 454 1536 1709 2579 Fragments arranged by size: 2579 2078 1709 1536 454 Bpu10I CC'TnA_GC Cuts at: 4269 4269 Size: 8356 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3087 3185 4931 5190 5564 6481 7346 7644 3087 Size: 98 1746 259 374 917 865 298 3799 Fragments arranged by size: 3799 1746 917 865 374 298 259 98 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4163 7019 882 Size: 617 2664 2856 2219 Fragments arranged by size: 2856 2664 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6147 493 Size: 711 4943 2702 Fragments arranged by size: 4943 2702 711 BsaBI GATnn'nnATC Cuts at: 8350 8350 Size: 8356 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6163 6545 7180 275 Size: 53 83 186 936 4630 382 635 1451 Fragments arranged by size: 4630 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2265 6789 7187 7461 7608 8019 2265 Size: 4524 398 274 147 411 2602 Fragments arranged by size: 4524 2602 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8131 Fragments arranged by size: 8131 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 8356 BseYI C'CCAG_C Cuts at: 3924 4173 7559 8055 3924 Size: 249 3386 496 4225 Fragments arranged by size: 4225 3386 496 249 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7783 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5806 6567 7595 1540 Size: 275 186 3805 761 1028 2301 Fragments arranged by size: 3805 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3994 6417 6502 7573 3994 Size: 2423 85 1071 4777 Fragments arranged by size: 4777 2423 1071 85 BsmI GAATG_Cn' Cuts at: 3439 5867 5960 3439 Size: 2428 93 5835 Fragments arranged by size: 5835 2428 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2656 2656 Size: 8356 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2671 3994 5675 6417 6502 7573 1391 Size: 399 881 1323 1681 742 85 1071 2174 Fragments arranged by size: 2174 1681 1323 1071 881 742 399 85 BspEI T'CCGG_A Cuts at: 8019 8019 Size: 8356 BspHI T'CATG_A Cuts at: 4753 6245 7975 4753 Size: 1492 1730 5134 Fragments arranged by size: 5134 1730 1492 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2008 4110 5711 844 Size: 1164 2102 1601 3489 Fragments arranged by size: 3489 2102 1601 1164 BsrBI CCG'CTC Cuts at: 1394 1823 6243 1394 Size: 429 4420 3507 Fragments arranged by size: 4420 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6851 7025 65 Size: 1183 5603 174 1396 Fragments arranged by size: 5603 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2265 7000 7187 1700 Size: 110 455 4735 187 2869 Fragments arranged by size: 4735 2869 455 187 110 BsrGI T'GTAC_A Cuts at: 96 2007 5500 96 Size: 1911 3493 2952 Fragments arranged by size: 3493 2952 1911 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8356 BssSI C'ACGA_G Cuts at: 2217 2709 6416 7428 2217 Size: 492 3707 1012 3145 Fragments arranged by size: 3707 3145 1012 492 BstAPI GCAn_nnn'nTGC Cuts at: 794 3001 3455 4795 794 Size: 2207 454 1340 4355 Fragments arranged by size: 4355 2207 1340 454 BstBI TT'CG_AA Cuts at: 2038 2299 4903 7160 2038 Size: 261 2604 2257 3234 Fragments arranged by size: 3234 2604 2257 261 BstEII G'GTnAC_C Cuts at: 1184 2268 3968 1184 Size: 1084 1700 5572 Fragments arranged by size: 5572 1700 1084 BstXI CCAn_nnnn'nTGG Cuts at: 5285 5285 Size: 8356 BstYI r'GATC_y Cuts at: 1972 2065 2108 3124 3322 3469 5686 6053 6438 Size: 93 43 1016 198 147 2217 367 385 Cuts at: 6438 6455 7907 7993 8005 8351 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2217 1977 1452 1016 385 367 346 198 147 93 86 43 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7804 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7617 Fragments arranged by size: 7617 683 56 BtsI GCAGTG_nn' Cuts at: 3660 4922 5266 5872 6666 6686 3660 Size: 1262 344 606 794 20 5330 Fragments arranged by size: 5330 1262 794 606 344 20 ClaI AT'CG_AT Cuts at: 1554 2277 5042 1554 Size: 723 2765 4868 Fragments arranged by size: 4868 2765 723 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8321 DraI TTT'AAA Cuts at: 3522 4189 5660 3522 Size: 667 1471 6218 Fragments arranged by size: 6218 1471 667 DraIII CAC_nnn'GTG Cuts at: 5654 5654 Size: 8356 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7363 809 Size: 507 6047 1802 Fragments arranged by size: 6047 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5619 5803 6692 Size: 54 1425 50 275 18 3789 184 889 Cuts at: 6692 8321 8 Size: 1629 43 Fragments arranged by size: 3789 1629 1425 889 275 184 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5803 1537 Size: 275 3991 4090 Fragments arranged by size: 4090 3991 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2296 2589 3423 4232 4382 4694 4809 5013 Size: 943 293 834 809 150 312 115 204 Cuts at: 5013 6286 1353 Size: 1273 3423 Fragments arranged by size: 3423 1273 943 834 809 312 293 204 150 115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6943 7317 7463 387 Size: 1064 138 5354 374 146 1280 Fragments arranged by size: 5354 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2056 3294 3317 3771 4409 4679 5107 Size: 393 424 1238 23 454 638 270 428 Cuts at: 5107 5307 6419 7016 7803 8071 1239 Size: 200 1112 597 787 268 1524 Fragments arranged by size: 1524 1238 1112 787 638 597 454 428 424 393 270 268 200 23 EcoRI G'AATT_C Cuts at: 5665 6043 5665 Size: 378 7978 EcoRV GAT'ATC Cuts at: 2812 2812 Size: 8356 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2312 2424 2891 2312 Size: 112 467 7777 Fragments arranged by size: 7777 467 112 FspI TGC'GCA Cuts at: 4106 6862 4106 Size: 2756 5600 HaeII r_GCGC'y Cuts at: 1678 2388 4714 7503 1678 Size: 710 2326 2789 2531 Fragments arranged by size: 2789 2531 2326 710 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3835 3867 4768 4800 6997 Size: 32 135 32 2263 32 901 32 2197 Cuts at: 6997 7029 7071 7103 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2263 2197 901 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4421 4951 5097 5275 5700 5946 Size: 988 281 2483 530 146 178 425 246 Cuts at: 5946 669 Size: 3079 Fragments arranged by size: 3079 2483 988 530 425 281 246 178 146 HindIII A'AGCT_T Cuts at: 748 3867 4300 748 Size: 3119 433 4804 Fragments arranged by size: 4804 3119 433 HpaI GTT'AAC Cuts at: 4951 5097 5946 4951 Size: 146 849 7361 Fragments arranged by size: 7361 849 146 KpnI G_GTAC'C Cuts at: 5681 5681 Size: 8356 MfeI C'AATT_G Cuts at: 2058 5955 2058 Size: 3897 4459 MluI A'CGCG_T Cuts at: 8232 8232 Size: 8356 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2898 5272 7470 7654 1305 Size: 1593 2374 2198 184 2007 Fragments arranged by size: 2374 2198 2007 1593 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5621 10 Size: 54 1425 343 3789 2745 Fragments arranged by size: 3789 2745 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3650 3987 4740 5598 6314 6673 6832 Size: 648 2484 337 753 858 716 359 159 Cuts at: 6832 518 Size: 2042 Fragments arranged by size: 2484 2042 858 753 716 648 359 337 159 MspA1I CmG'CkG Cuts at: 1606 2185 3928 5552 5772 6449 7597 7842 1606 Size: 579 1743 1624 220 677 1148 245 2120 Fragments arranged by size: 2120 1743 1624 1148 677 579 245 220 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7804 NdeI CA'TA_TG Cuts at: 387 4737 387 Size: 4350 4006 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8356 NotI GC'GGCC_GC Cuts at: 5803 5803 Size: 8356 NspI r_CATG'y Cuts at: 1132 1229 3587 3651 3881 4310 4798 5507 5714 Size: 97 2358 64 230 429 488 709 207 Cuts at: 5714 7259 1132 Size: 1545 2229 Fragments arranged by size: 2358 2229 1545 709 488 429 230 207 97 64 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8356 PciI A'CATG_T Cuts at: 3877 7255 3877 Size: 3378 4978 PflMI CCAn_nnn'nTGG Cuts at: 1108 5449 1108 Size: 4341 4015 PfoI T'CCnGG_A Cuts at: 3748 3748 Size: 8356 PmeI GTTT'AAAC Cuts at: 5660 5660 Size: 8356 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6425 6457 7938 7970 6425 Size: 32 1481 32 6811 Fragments arranged by size: 6811 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8356 PsiI TTA'TAA Cuts at: 5926 5926 Size: 8356 PspXI vC'TCGA_Gb Cuts at: 3342 4482 3342 Size: 1140 7216 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5484 5516 5484 Size: 32 8324 PstI C_TGCA'G Cuts at: 830 5708 830 Size: 4878 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8356 PvuII CAG'CTG Cuts at: 3928 5552 3928 Size: 1624 6732 SalI G'TCGA_C Cuts at: 1936 5698 1936 Size: 3762 4594 SbfI CC_TGCA'GG Cuts at: 5708 5708 Size: 8356 ScaI AGT'ACT Cuts at: 6604 8316 6604 Size: 1712 6644 SexAI A'CCwGG_T Cuts at: 5149 5149 Size: 8356 SfcI C'TryA_G Cuts at: 826 1045 5704 6839 7520 7711 826 Size: 219 4659 1135 681 191 1471 Fragments arranged by size: 4659 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8356 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8356 SmaI CCC'GGG Cuts at: 1872 5683 1872 Size: 3811 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3102 3200 3342 4482 4910 5205 Size: 197 926 1159 98 142 1140 428 295 Cuts at: 5205 5543 6460 7361 7623 820 Size: 338 917 901 262 1553 Fragments arranged by size: 1553 1159 1140 926 917 901 428 338 295 262 197 142 98 SnaBI TAC'GTA Cuts at: 493 6147 493 Size: 5654 2702 SpeI A'CTAG_T Cuts at: 152 2747 152 Size: 2595 5761 SphI G_CATG'C Cuts at: 1132 4310 4798 5714 1132 Size: 3178 488 916 3774 Fragments arranged by size: 3774 3178 916 488 SspI AAT'ATT Cuts at: 5 52 3368 4114 6280 5 Size: 47 3316 746 2166 2081 Fragments arranged by size: 3316 2166 2081 746 47 StuI AGG'CCT Cuts at: 1517 5533 1517 Size: 4016 4340 StyI C'CwwG_G Cuts at: 513 1065 2931 5367 5795 513 Size: 552 1866 2436 428 3074 Fragments arranged by size: 3074 2436 1866 552 428 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6383 6400 6553 6738 7183 325 Size: 1255 4803 17 153 185 445 1498 Fragments arranged by size: 4803 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 4938 5062 Size: 274 80 33 51 1443 30 2931 124 Cuts at: 5062 5500 6602 8314 96 Size: 438 1102 1712 138 Fragments arranged by size: 2931 1712 1443 1102 438 274 138 124 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5286 5318 8316 8348 5286 Size: 32 2998 32 5294 Fragments arranged by size: 5294 2998 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3974 1316 Size: 2658 5698 XbaI T'CTAG_A Cuts at: 2611 4196 5692 2611 Size: 1585 1496 5275 Fragments arranged by size: 5275 1585 1496 XhoI C'TCGA_G Cuts at: 1943 3342 4482 1943 Size: 1399 1140 5817 Fragments arranged by size: 5817 1399 1140 XmaI C'CCGG_G Cuts at: 1870 5681 1870 Size: 3811 4545 XmnI GAAnn'nnTTC Cuts at: 4259 5088 6485 4259 Size: 829 1397 6130 Fragments arranged by size: 6130 1397 829 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6164 276 Size: 53 83 186 936 4630 2468 Fragments arranged by size: 4630 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI