(Circular) (Six-base) MAPSORT of: pFN21AB9271.seq Check: 1169 from: 1 to: 6469 pFN21AB9271 6469 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2234 2246 2234 Size: 12 6457 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4279 278 Size: 53 83 186 936 2743 2468 Fragments arranged by size: 2743 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3812 1937 Size: 1875 4594 Acc65I G'GTAC_C Cuts at: 3790 3790 Size: 6469 AclI AA'CG_TT Cuts at: 1563 4596 4969 1563 Size: 3033 373 3063 Fragments arranged by size: 3063 3033 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3703 4532 5916 6184 1632 Size: 2071 829 1384 268 1917 Fragments arranged by size: 2071 1917 1384 829 268 AflII C'TTAA_G Cuts at: 820 1017 2497 820 Size: 197 1480 4792 Fragments arranged by size: 4792 1480 197 AflIII A'CryG_T Cuts at: 1203 5368 6345 1203 Size: 4165 977 1327 Fragments arranged by size: 4165 1327 977 AgeI A'CCGG_T Cuts at: 5300 5300 Size: 6469 AhdI GACnn_n'nnGTC Cuts at: 5198 5198 Size: 6469 AleI CACnn'nnGTG Cuts at: 1166 3342 1166 Size: 2176 4293 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6469 AlwNI CAG_nnn'CTG Cuts at: 2203 4212 5784 2203 Size: 2009 1572 2888 Fragments arranged by size: 2888 2009 1572 ApaI G_GGCC'C Cuts at: 2644 2644 Size: 6469 ApaLI G'TGCA_C Cuts at: 4526 5682 4526 Size: 1156 5313 ApoI r'AATT_y Cuts at: 1493 2391 3565 3778 4004 4156 6265 1493 Size: 898 1174 213 226 152 2109 1697 Fragments arranged by size: 2109 1697 1174 898 226 213 152 AseI AT'TA_AT Cuts at: 160 1032 5023 160 Size: 872 3991 1606 Fragments arranged by size: 3991 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 AvaI C'yCGr_G Cuts at: 1870 1943 2922 3794 1870 Size: 73 979 872 4545 Fragments arranged by size: 4545 979 872 73 BamHI G'GATC_C Cuts at: 3585 3799 4166 3585 Size: 214 367 5888 Fragments arranged by size: 5888 367 214 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 2076 3790 5245 618 Size: 325 217 627 224 65 1714 1455 1842 Fragments arranged by size: 1842 1714 1455 627 325 224 217 65 BanII G_rGCy'C Cuts at: 1391 2644 3788 1391 Size: 1253 1144 4072 Fragments arranged by size: 4072 1253 1144 BbeI G_GCGC'C Cuts at: 2015 2015 Size: 6469 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2046 928 Size: 971 147 5351 Fragments arranged by size: 5351 971 147 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4660 4694 1903 Size: 34 2723 34 3678 Fragments arranged by size: 3678 2723 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4361 5571 4361 Size: 1210 5259 BclI T'GATC_A Cuts at: 1548 1548 Size: 6469 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5080 136 Size: 107 122 71 631 605 337 3071 1525 Fragments arranged by size: 3071 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6464 6464 Size: 6469 BlpI GC'TnA_GC Cuts at: 3886 3886 Size: 6469 Bme1580I G_kGCm'C Cuts at: 1790 2079 2644 4530 5686 1790 Size: 289 565 1886 1156 2573 Fragments arranged by size: 2573 1886 1156 565 289 BmgBI CAC'GTC Cuts at: 1318 2044 1318 Size: 726 5743 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2156 2763 4200 Size: 416 53 83 399 24 739 607 1437 Cuts at: 4200 5158 442 Size: 958 1753 Fragments arranged by size: 1753 1437 958 739 607 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6469 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2326 2566 2326 Size: 240 6229 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2082 2141 2691 3195 3531 3615 3699 5129 Size: 843 59 550 504 336 84 84 1430 Cuts at: 5129 1239 Size: 2579 Fragments arranged by size: 2579 1430 843 550 504 336 84 84 59 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2326 2428 2538 4594 5459 5757 2326 Size: 102 110 2056 865 298 3038 Fragments arranged by size: 3038 2056 865 298 110 102 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2117 2146 5132 882 Size: 617 618 29 2986 2219 Fragments arranged by size: 2986 2219 618 617 29 BsaAI yAC'GTr Cuts at: 493 1204 4260 493 Size: 711 3056 2702 Fragments arranged by size: 3056 2702 711 BsaBI GATnn'nnATC Cuts at: 6463 6463 Size: 6469 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 4276 4658 5293 Size: 53 83 186 936 479 2264 382 635 Cuts at: 5293 275 Size: 1451 Fragments arranged by size: 2264 1451 936 635 479 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2837 3257 4902 5300 5574 5721 6132 2837 Size: 420 1645 398 274 147 411 3174 Fragments arranged by size: 3174 1645 420 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2957 2987 1178 Size: 30 165 30 1554 30 4660 Fragments arranged by size: 4660 1554 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2965 1186 Size: 1779 4690 BseYI C'CCAG_C Cuts at: 5672 6168 5672 Size: 496 5973 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3382 1163 Size: 573 1646 4250 Fragments arranged by size: 4250 1646 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3919 4680 5708 1540 Size: 275 186 1918 761 1028 2301 Fragments arranged by size: 2301 1918 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2823 4530 4615 5686 2823 Size: 1707 85 1071 3606 Fragments arranged by size: 3606 1707 1071 85 BsmI GAATG_Cn' Cuts at: 2876 3617 3701 3980 4073 2876 Size: 741 84 279 93 5272 Fragments arranged by size: 5272 741 279 93 84 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2644 2823 3788 4530 4615 5686 Size: 399 289 565 179 965 742 85 1071 Cuts at: 5686 1391 Size: 2174 Fragments arranged by size: 2174 1071 965 742 565 399 289 179 85 BspEI T'CCGG_A Cuts at: 6132 6132 Size: 6469 BspHI T'CATG_A Cuts at: 4358 6088 4358 Size: 1730 4739 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2135 2234 2246 2793 3568 3824 844 Size: 1291 99 12 547 775 256 3489 Fragments arranged by size: 3489 1291 775 547 256 99 12 BsrBI CCG'CTC Cuts at: 1394 1823 2889 3057 4356 1394 Size: 429 1066 168 1299 3507 Fragments arranged by size: 3507 1299 1066 429 168 BsrDI GCAATG_nn' Cuts at: 65 1248 2698 3706 4964 5138 65 Size: 1183 1450 1008 1258 174 1396 Fragments arranged by size: 1450 1396 1258 1183 1008 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2014 5113 5300 1700 Size: 110 204 3099 187 2869 Fragments arranged by size: 3099 2869 204 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6469 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6469 BssSI C'ACGA_G Cuts at: 4529 5541 4529 Size: 1012 5457 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6469 BstBI TT'CG_AA Cuts at: 5273 5273 Size: 6469 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6469 BstYI r'GATC_y Cuts at: 1972 2336 3585 3799 4166 4551 4568 6020 6106 Size: 364 1249 214 367 385 17 1452 86 Cuts at: 6106 6118 6464 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1249 385 367 364 346 214 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4978 Fragments arranged by size: 4978 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3206 487 Size: 683 56 1980 3750 Fragments arranged by size: 3750 1980 683 56 BtsI GCAGTG_nn' Cuts at: 3370 3985 4779 4799 3370 Size: 615 794 20 5040 Fragments arranged by size: 5040 794 615 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6469 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2680 2715 2848 2883 2932 2967 3268 Size: 35 2116 35 133 35 49 35 301 Cuts at: 3268 3303 3688 3723 529 Size: 35 385 35 3275 Fragments arranged by size: 3275 2116 385 301 133 49 35 35 35 35 35 35 DraI TTT'AAA Cuts at: 3773 3773 Size: 6469 DraIII CAC_nnn'GTG Cuts at: 3079 3079 Size: 6469 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5476 809 Size: 507 4160 1802 Fragments arranged by size: 4160 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3916 4805 6434 Size: 54 1425 50 275 18 2086 889 1629 Cuts at: 6434 8 Size: 43 Fragments arranged by size: 2086 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3916 1537 Size: 275 2104 4090 Fragments arranged by size: 4090 2104 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2210 2896 3316 3484 4399 1353 Size: 857 686 420 168 915 3423 Fragments arranged by size: 3423 915 857 686 420 168 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5056 5430 5576 387 Size: 1064 138 3467 374 146 1280 Fragments arranged by size: 3467 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2082 2141 2691 3195 3531 3615 3699 Size: 393 450 59 550 504 336 84 84 Cuts at: 3699 3703 4532 5129 5916 6184 1239 Size: 4 829 597 787 268 1524 Fragments arranged by size: 1524 829 787 597 550 504 450 393 336 268 84 84 59 4 EcoNI CCTnn'n_nnAGG Cuts at: 2105 2105 Size: 6469 EcoO109I rG'GnC_Cy Cuts at: 2097 2641 3249 3417 2097 Size: 544 608 168 5149 Fragments arranged by size: 5149 608 544 168 EcoRI G'AATT_C Cuts at: 3778 4156 3778 Size: 378 6091 FspI TGC'GCA Cuts at: 2242 4975 2242 Size: 2733 3736 HaeII r_GCGC'y Cuts at: 1678 2015 5616 1678 Size: 337 3601 2531 Fragments arranged by size: 3601 2531 337 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2905 2937 5110 5142 5184 Size: 32 135 32 1333 32 2173 32 42 Cuts at: 5184 5216 1373 Size: 32 2626 Fragments arranged by size: 2626 2173 1333 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3813 4059 669 Size: 988 281 1875 246 3079 Fragments arranged by size: 3079 1875 988 281 246 HindIII A'AGCT_T Cuts at: 748 2958 748 Size: 2210 4259 HpaI GTT'AAC Cuts at: 4059 4059 Size: 6469 KasI G'GCGC_C Cuts at: 2011 2011 Size: 6469 KpnI G_GTAC'C Cuts at: 3794 3794 Size: 6469 MfeI C'AATT_G Cuts at: 4068 4068 Size: 6469 MluI A'CGCG_T Cuts at: 6345 6345 Size: 6469 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3262 5583 5767 1305 Size: 1957 2321 184 2007 Fragments arranged by size: 2321 2007 1957 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4647 Fragments arranged by size: 4647 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2544 2770 3106 3342 4427 4786 4945 Size: 648 1378 226 336 236 1085 359 159 Cuts at: 4945 518 Size: 2042 Fragments arranged by size: 2042 1378 1085 648 359 336 236 226 159 MspA1I CmG'CkG Cuts at: 1606 2290 3885 4562 5710 5955 1606 Size: 684 1595 677 1148 245 2120 Fragments arranged by size: 2120 1595 1148 684 677 245 NaeI GCC'GGC Cuts at: 2016 2016 Size: 6469 NarI GG'CG_CC Cuts at: 2012 2012 Size: 6469 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4978 Fragments arranged by size: 4978 939 552 NdeI CA'TA_TG Cuts at: 387 2739 2991 3327 387 Size: 2352 252 336 3529 Fragments arranged by size: 3529 2352 336 252 NgoMIV G'CCGG_C Cuts at: 2014 2014 Size: 6469 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6469 NotI GC'GGCC_GC Cuts at: 3916 3916 Size: 6469 NspI r_CATG'y Cuts at: 1132 1229 2607 3827 5372 1132 Size: 97 1378 1220 1545 2229 Fragments arranged by size: 2229 1545 1378 1220 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6469 PciI A'CATG_T Cuts at: 5368 5368 Size: 6469 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6469 PfoI T'CCnGG_A Cuts at: 2316 2316 Size: 6469 PmeI GTTT'AAAC Cuts at: 3773 3773 Size: 6469 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4538 4570 6051 6083 4538 Size: 32 1481 32 4924 Fragments arranged by size: 4924 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2097 3249 3417 2097 Size: 1152 168 5149 Fragments arranged by size: 5149 1152 168 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6469 PsiI TTA'TAA Cuts at: 2460 4039 2460 Size: 1579 4890 PspOMI G'GGCC_C Cuts at: 2640 2640 Size: 6469 PstI C_TGCA'G Cuts at: 830 2132 3821 830 Size: 1302 1689 3478 Fragments arranged by size: 3478 1689 1302 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6469 PvuII CAG'CTG Cuts at: 2290 2290 Size: 6469 SalI G'TCGA_C Cuts at: 1936 3811 1936 Size: 1875 4594 SapI GCTCTTCn'nnn_ Cuts at: 2896 3316 3484 2896 Size: 420 168 5881 Fragments arranged by size: 5881 420 168 SbfI CC_TGCA'GG Cuts at: 2132 3821 2132 Size: 1689 4780 ScaI AGT'ACT Cuts at: 4717 6429 4717 Size: 1712 4757 SfcI C'TryA_G Cuts at: 826 1045 2128 2968 3472 3817 4952 5633 5824 Size: 219 1083 840 504 345 1135 681 191 Cuts at: 5824 826 Size: 1471 Fragments arranged by size: 1471 1135 1083 840 681 504 345 219 191 SfoI GGC'GCC Cuts at: 2013 2013 Size: 6469 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 SgrAI Cr'CCGG_yG Cuts at: 1700 2014 1700 Size: 314 6155 SmaI CCC'GGG Cuts at: 1872 3796 1872 Size: 1924 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2341 2407 2497 2553 2922 4573 Size: 197 926 398 66 90 56 369 1651 Cuts at: 4573 5474 5736 820 Size: 901 262 1553 Fragments arranged by size: 1651 1553 926 901 398 369 262 197 90 66 56 SnaBI TAC'GTA Cuts at: 493 4260 493 Size: 3767 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6469 SphI G_CATG'C Cuts at: 1132 3827 1132 Size: 2695 3774 SspI AAT'ATT Cuts at: 5 52 4393 5 Size: 47 4341 2081 Fragments arranged by size: 4341 2081 47 StuI AGG'CCT Cuts at: 1517 2223 3044 3212 3296 1517 Size: 706 821 168 84 4690 Fragments arranged by size: 4690 821 706 168 84 StyI C'CwwG_G Cuts at: 513 1065 2004 3438 3908 513 Size: 552 939 1434 470 3074 Fragments arranged by size: 3074 1434 939 552 470 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2897 4496 4513 4666 4851 5296 325 Size: 1255 1317 1599 17 153 185 445 1498 Fragments arranged by size: 1599 1498 1317 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4715 6427 96 Size: 274 80 33 51 1443 2738 1712 138 Fragments arranged by size: 2738 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3409 3441 6429 6461 3409 Size: 32 2988 32 3417 Fragments arranged by size: 3417 2988 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6469 XbaI T'CTAG_A Cuts at: 3805 3805 Size: 6469 XhoI C'TCGA_G Cuts at: 1943 2922 1943 Size: 979 5490 XmaI C'CCGG_G Cuts at: 1870 3794 1870 Size: 1924 4545 XmnI GAAnn'nnTTC Cuts at: 2792 3632 4598 2792 Size: 840 966 4663 Fragments arranged by size: 4663 966 840 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4277 276 Size: 53 83 186 936 2743 2468 Fragments arranged by size: 2743 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI