Restriction map for
pFN21AB5474
(Circular) (Six-base) MAPSORT of: pFN21AB5474.seq Check: 7764 from: 1 to: 5443
pFN21AB5474 5443 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3253 278
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2786 1937
Size: 849 4594
Acc65I G'GTAC_C
Cuts at: 2764 2764
Size: 5443
AclI AA'CG_TT
Cuts at: 1563 2742 3570 3943 1563
Size: 1179 828 373 3063
Fragments arranged by size:
3063 1179 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2421 3506 4890 5158 1632
Size: 789 1085 1384 268 1917
Fragments arranged by size:
1917 1384 1085 789 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5246
AflIII A'CryG_T
Cuts at: 1203 4342 5319 1203
Size: 3139 977 1327
Fragments arranged by size:
3139 1327 977
AgeI A'CCGG_T
Cuts at: 4274 4274
Size: 5443
AhdI GACnn_n'nnGTC
Cuts at: 4172 4172
Size: 5443
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5443
AlwNI CAG_nnn'CTG
Cuts at: 3186 4758 3186
Size: 1572 3871
ApaLI G'TGCA_C
Cuts at: 3500 4656 3500
Size: 1156 4287
ApoI r'AATT_y
Cuts at: 1493 2527 2752 2978 3130 5239 1493
Size: 1034 225 226 152 2109 1697
Fragments arranged by size:
2109 1697 1034 226 225 152
AseI AT'TA_AT
Cuts at: 160 1032 3997 160
Size: 872 2965 1606
Fragments arranged by size:
2965 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
AvaI C'yCGr_G
Cuts at: 1870 1943 2768 1870
Size: 73 825 4545
Fragments arranged by size:
4545 825 73
BamHI G'GATC_C
Cuts at: 2773 3140 2773
Size: 367 5076
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2168 2764 4219 618
Size: 325 217 627 381 596 1455 1842
Fragments arranged by size:
1842 1455 627 596 381 325 217
BanII G_rGCy'C
Cuts at: 1391 2762 1391
Size: 1371 4072
BbeI G_GCGC'C
Cuts at: 2172 2172
Size: 5443
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4472
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3634 3668 1903
Size: 34 1697 34 3678
Fragments arranged by size:
3678 1697 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2614 3335 4545 2614
Size: 721 1210 3512
Fragments arranged by size:
3512 1210 721
BclI T'GATC_A
Cuts at: 1548 2658 1548
Size: 1110 4333
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2090 4054 136
Size: 107 122 71 631 605 418 1964 1525
Fragments arranged by size:
1964 1525 631 605 418 122 107 71
BglII A'GATC_T
Cuts at: 5438 5438
Size: 5443
BlpI GC'TnA_GC
Cuts at: 2049 2058 2860 2049
Size: 9 802 4632
Fragments arranged by size:
4632 802 9
Bme1580I G_kGCm'C
Cuts at: 1790 3504 4660 1790
Size: 1714 1156 2573
Fragments arranged by size:
2573 1714 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5443
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3174 4132 442
Size: 416 53 83 399 24 1757 958 1753
Fragments arranged by size:
1757 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5443
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2182 2324 2433 4103 1239
Size: 943 142 109 1670 2579
Fragments arranged by size:
2579 1670 943 142 109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3568 4433 4731 3568
Size: 865 298 4280
Fragments arranged by size:
4280 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4106 882
Size: 617 2607 2219
Fragments arranged by size:
2607 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3234 493
Size: 711 2030 2702
Fragments arranged by size:
2702 2030 711
BsaBI GATnn'nnATC
Cuts at: 5437 5437
Size: 5443
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2169 3250 3632 4267
Size: 53 83 186 936 636 1081 382 635
Cuts at: 4267 275
Size: 1451
Fragments arranged by size:
1451 1081 936 636 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3876 4274 4548 4695 5106 3876
Size: 398 274 147 411 4213
Fragments arranged by size:
4213 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2727 2757 1178
Size: 30 165 30 1324 30 3864
Fragments arranged by size:
3864 1324 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5443
BseYI C'CCAG_C
Cuts at: 2445 2726 4646 5142 2445
Size: 281 1920 496 2746
Fragments arranged by size:
2746 1920 496 281
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4870
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2893 3654 4682 1540
Size: 275 186 892 761 1028 2301
Fragments arranged by size:
2301 1028 892 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3504 3589 4660 3504
Size: 85 1071 4287
Fragments arranged by size:
4287 1071 85
BsmI GAATG_Cn'
Cuts at: 2954 3047 2954
Size: 93 5350
BsmBI CGTCTCn'nnnn_
Cuts at: 2706 2715 2706
Size: 9 5434
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2762 3504 3589 4660 1391
Size: 399 972 742 85 1071 2174
Fragments arranged by size:
2174 1071 972 742 399 85
BspEI T'CCGG_A
Cuts at: 5106 5106
Size: 5443
BspHI T'CATG_A
Cuts at: 3332 5062 3332
Size: 1730 3713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2798 844
Size: 1954 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2178 3330 1394
Size: 429 355 1152 3507
Fragments arranged by size:
3507 1152 429 355
BsrDI GCAATG_nn'
Cuts at: 65 1248 2299 3938 4112 65
Size: 1183 1051 1639 174 1396
Fragments arranged by size:
1639 1396 1183 1051 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4087 4274 1700
Size: 110 2277 187 2869
Fragments arranged by size:
2869 2277 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5443
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5443
BssSI C'ACGA_G
Cuts at: 3503 4515 3503
Size: 1012 4431
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5443
BstBI TT'CG_AA
Cuts at: 4247 4247
Size: 5443
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5443
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5443
BstYI r'GATC_y
Cuts at: 1972 2773 3140 3525 3542 4994 5080 5092 5438
Size: 801 367 385 17 1452 86 12 346
Cuts at: 5438 1972
Size: 1977
Fragments arranged by size:
1977 1452 801 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4891
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4704
Fragments arranged by size:
4704 683 56
BtsI GCAGTG_nn'
Cuts at: 2959 3753 3773 2959
Size: 794 20 4629
Fragments arranged by size:
4629 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5443
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5408
DraI TTT'AAA
Cuts at: 2747 2747
Size: 5443
DraIII CAC_nnn'GTG
Cuts at: 2691 2691
Size: 5443
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4450 809
Size: 507 3134 1802
Fragments arranged by size:
3134 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2890 3779 5408
Size: 54 1425 50 275 18 1060 889 1629
Cuts at: 5408 8
Size: 43
Fragments arranged by size:
1629 1425 1060 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2890 1537
Size: 275 1078 4090
Fragments arranged by size:
4090 1078 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2121 2185 2628 3373 1353
Size: 768 64 443 745 3423
Fragments arranged by size:
3423 768 745 443 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4030 4404 4550 387
Size: 1064 138 2441 374 146 1280
Fragments arranged by size:
2441 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2182 2324 2421 2433 3506 4103 4890
Size: 393 550 142 97 12 1073 597 787
Cuts at: 4890 5158 1239
Size: 268 1524
Fragments arranged by size:
1524 1073 787 597 550 393 268 142
97 12
EcoRI G'AATT_C
Cuts at: 2752 3130 2752
Size: 378 5065
FspI TGC'GCA
Cuts at: 3949 3949
Size: 5443
HaeII r_GCGC'y
Cuts at: 1678 2172 4590 1678
Size: 494 2418 2531
Fragments arranged by size:
2531 2418 494
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4084 4116 4158 4190 1373
Size: 32 135 32 2512 32 42 32 2626
Fragments arranged by size:
2626 2512 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2787 3033 669
Size: 988 281 849 246 3079
Fragments arranged by size:
3079 988 849 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5443
HpaI GTT'AAC
Cuts at: 3033 3033
Size: 5443
KasI G'GCGC_C
Cuts at: 2168 2168
Size: 5443
KpnI G_GTAC'C
Cuts at: 2768 2768
Size: 5443
MfeI C'AATT_G
Cuts at: 2607 3042 2607
Size: 435 5008
MluI A'CGCG_T
Cuts at: 5319 5319
Size: 5443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2145 2292 4557 4741 1305
Size: 840 147 2265 184 2007
Fragments arranged by size:
2265 2007 840 184 147
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3621
Fragments arranged by size:
3621 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3401 3760 3919 518
Size: 648 843 1392 359 159 2042
Fragments arranged by size:
2042 1392 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2859 3536 4684 4929 1606
Size: 1253 677 1148 245 2120
Fragments arranged by size:
2120 1253 1148 677 245
NarI GG'CG_CC
Cuts at: 2169 2169
Size: 5443
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4891
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5443
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5443
NotI GC'GGCC_GC
Cuts at: 2890 2890
Size: 5443
NspI r_CATG'y
Cuts at: 1132 1229 2801 4346 1132
Size: 97 1572 1545 2229
Fragments arranged by size:
2229 1572 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5443
PciI A'CATG_T
Cuts at: 4342 4342
Size: 5443
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5443
PmeI GTTT'AAAC
Cuts at: 2747 2747
Size: 5443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3512 3544 5025 5057 3512
Size: 32 1481 32 3898
Fragments arranged by size:
3898 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5443
PsiI TTA'TAA
Cuts at: 2643 3013 2643
Size: 370 5073
PstI C_TGCA'G
Cuts at: 830 2083 2283 2795 830
Size: 1253 200 512 3478
Fragments arranged by size:
3478 1253 512 200
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5443
SalI G'TCGA_C
Cuts at: 1936 2785 1936
Size: 849 4594
SapI GCTCTTCn'nnn_
Cuts at: 2185 2185
Size: 5443
SbfI CC_TGCA'GG
Cuts at: 2795 2795
Size: 5443
ScaI AGT'ACT
Cuts at: 3691 5403 3691
Size: 1712 3731
SfcI C'TryA_G
Cuts at: 826 1045 2079 2279 2575 2791 3926 4607 4798
Size: 219 1034 200 296 216 1135 681 191
Cuts at: 4798 826
Size: 1471
Fragments arranged by size:
1471 1135 1034 681 296 219 216 200
191
SfoI GGC'GCC
Cuts at: 2170 2170
Size: 5443
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5443
SmaI CCC'GGG
Cuts at: 1872 2770 1872
Size: 898 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3547 4448 4710 820
Size: 197 926 1604 901 262 1553
Fragments arranged by size:
1604 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3234 493
Size: 2741 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5443
SphI G_CATG'C
Cuts at: 1132 2801 1132
Size: 1669 3774
SspI AAT'ATT
Cuts at: 5 52 3367 5
Size: 47 3315 2081
Fragments arranged by size:
3315 2081 47
StuI AGG'CCT
Cuts at: 1517 2573 1517
Size: 1056 4387
StyI C'CwwG_G
Cuts at: 513 1065 2882 513
Size: 552 1817 3074
Fragments arranged by size:
3074 1817 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2517 3470 3487 3640 3825 4270 325
Size: 1255 937 953 17 153 185 445 1498
Fragments arranged by size:
1498 1255 953 937 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3689 5401 96
Size: 274 80 33 51 1443 1712 1712 138
Fragments arranged by size:
1712 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5403 5435 5403
Size: 32 5411
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5443
XbaI T'CTAG_A
Cuts at: 2779 2779
Size: 5443
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5443
XmaI C'CCGG_G
Cuts at: 1870 2768 1870
Size: 898 4545
XmnI GAAnn'nnTTC
Cuts at: 3572 3572
Size: 5443
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3251 276
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI
SwaI XcmI