(Circular) (Six-base) MAPSORT of: pFN21AB5452.seq Check: 4794 from: 1 to: 5971 pFN21AB5452 5971 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3781 278 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3314 1937 Size: 1377 4594 Acc65I G'GTAC_C Cuts at: 3292 3292 Size: 5971 AclI AA'CG_TT Cuts at: 1563 4098 4471 1563 Size: 2535 373 3063 Fragments arranged by size: 3063 2535 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 1994 2825 4034 5418 5686 1632 Size: 362 831 1209 1384 268 1917 Fragments arranged by size: 1917 1384 1209 831 362 268 AflII C'TTAA_G Cuts at: 820 1017 2581 820 Size: 197 1564 4210 Fragments arranged by size: 4210 1564 197 AflIII A'CryG_T Cuts at: 1203 2766 3268 4870 5847 1203 Size: 1563 502 1602 977 1327 Fragments arranged by size: 1602 1563 1327 977 502 AgeI A'CCGG_T Cuts at: 4802 4802 Size: 5971 AhdI GACnn_n'nnGTC Cuts at: 4700 4700 Size: 5971 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5971 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5971 AlwNI CAG_nnn'CTG Cuts at: 2486 3714 5286 2486 Size: 1228 1572 3171 Fragments arranged by size: 3171 1572 1228 ApaLI G'TGCA_C Cuts at: 4028 5184 4028 Size: 1156 4815 ApoI r'AATT_y Cuts at: 1493 2398 3280 3506 3658 5767 1493 Size: 905 882 226 152 2109 1697 Fragments arranged by size: 2109 1697 905 882 226 152 AseI AT'TA_AT Cuts at: 160 1032 2424 4525 160 Size: 872 1392 2101 1606 Fragments arranged by size: 2101 1606 1392 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 AvaI C'yCGr_G Cuts at: 1870 1943 3296 1870 Size: 73 1353 4545 Fragments arranged by size: 4545 1353 73 BamHI G'GATC_C Cuts at: 3301 3668 3301 Size: 367 5604 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3292 4747 618 Size: 325 217 627 1505 1455 1842 Fragments arranged by size: 1842 1505 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3290 1391 Size: 1899 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2029 2926 928 Size: 971 130 897 3973 Fragments arranged by size: 3973 971 897 130 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4162 4196 1903 Size: 34 50 34 2141 34 3678 Fragments arranged by size: 3678 2141 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3014 3863 5073 3014 Size: 849 1210 3912 Fragments arranged by size: 3912 1210 849 BclI T'GATC_A Cuts at: 1548 1548 Size: 5971 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4582 136 Size: 107 122 71 631 605 2910 1525 Fragments arranged by size: 2910 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2812 2968 5966 2812 Size: 156 2998 2817 Fragments arranged by size: 2998 2817 156 BlpI GC'TnA_GC Cuts at: 3388 3388 Size: 5971 Bme1580I G_kGCm'C Cuts at: 1790 4032 5188 1790 Size: 2242 1156 2573 Fragments arranged by size: 2573 2242 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5971 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2722 3702 4660 Size: 416 53 83 399 24 1305 980 958 Cuts at: 4660 442 Size: 1753 Fragments arranged by size: 1753 1305 980 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5971 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2360 2985 4631 1239 Size: 1121 625 1646 2579 Fragments arranged by size: 2579 1646 1121 625 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4096 4961 5259 4096 Size: 865 298 4808 Fragments arranged by size: 4808 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2070 4634 882 Size: 617 571 2564 2219 Fragments arranged by size: 2564 2219 617 571 BsaAI yAC'GTr Cuts at: 493 1204 2673 3762 493 Size: 711 1469 1089 2702 Fragments arranged by size: 2702 1469 1089 711 BsaBI GATnn'nnATC Cuts at: 5965 5965 Size: 5971 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3778 4160 4795 275 Size: 53 83 186 936 2245 382 635 1451 Fragments arranged by size: 2245 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4404 4802 5076 5223 5634 4404 Size: 398 274 147 411 4741 Fragments arranged by size: 4741 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2219 2249 1178 Size: 30 165 30 816 30 4900 Fragments arranged by size: 4900 816 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2032 1186 Size: 846 5125 BseYI C'CCAG_C Cuts at: 5174 5670 5174 Size: 496 5475 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5398 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3421 4182 5210 1540 Size: 275 186 1420 761 1028 2301 Fragments arranged by size: 2301 1420 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4032 4117 5188 4032 Size: 85 1071 4815 Fragments arranged by size: 4815 1071 85 BsmI GAATG_Cn' Cuts at: 2059 3482 3575 2059 Size: 1423 93 4455 Fragments arranged by size: 4455 1423 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3290 4032 4117 5188 1391 Size: 399 1500 742 85 1071 2174 Fragments arranged by size: 2174 1500 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5634 5634 Size: 5971 BspHI T'CATG_A Cuts at: 2140 2641 3860 5590 2140 Size: 501 1219 1730 2521 Fragments arranged by size: 2521 1730 1219 501 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3237 3326 844 Size: 2393 89 3489 Fragments arranged by size: 3489 2393 89 BsrBI CCG'CTC Cuts at: 1394 1823 3858 1394 Size: 429 2035 3507 Fragments arranged by size: 3507 2035 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2536 4466 4640 65 Size: 1183 1288 1930 174 1396 Fragments arranged by size: 1930 1396 1288 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4615 4802 1700 Size: 110 2805 187 2869 Fragments arranged by size: 2869 2805 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5971 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5971 BssSI C'ACGA_G Cuts at: 4031 5043 4031 Size: 1012 4959 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5971 BstBI TT'CG_AA Cuts at: 4775 4775 Size: 5971 BstEII G'GTnAC_C Cuts at: 1184 2843 1184 Size: 1659 4312 BstYI r'GATC_y Cuts at: 1972 2812 2968 3039 3301 3668 4053 4070 5522 Size: 840 156 71 262 367 385 17 1452 Cuts at: 5522 5608 5620 5966 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 840 385 367 346 262 156 86 71 17 12 Bsu36I CC'TnA_GG Cuts at: 2276 2276 Size: 5971 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5419 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5232 Fragments arranged by size: 5232 683 56 BtsI GCAGTG_nn' Cuts at: 2026 3487 4281 4301 2026 Size: 1461 794 20 3696 Fragments arranged by size: 3696 1461 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5971 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5936 DraI TTT'AAA Cuts at: 2989 3275 2989 Size: 286 5685 DraIII CAC_nnn'GTG Cuts at: 2354 2354 Size: 5971 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2771 4978 809 Size: 507 1455 2207 1802 Fragments arranged by size: 2207 1802 1455 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3418 4307 5936 Size: 54 1425 50 275 18 1588 889 1629 Cuts at: 5936 8 Size: 43 Fragments arranged by size: 1629 1588 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3418 1537 Size: 275 1606 4090 Fragments arranged by size: 4090 1606 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2500 3901 1353 Size: 1147 1401 3423 Fragments arranged by size: 3423 1401 1147 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2996 4558 4932 5078 387 Size: 1064 138 1407 1562 374 146 1280 Fragments arranged by size: 1562 1407 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1994 2360 2825 2985 4034 4631 5418 Size: 393 362 366 465 160 1049 597 787 Cuts at: 5418 5686 1239 Size: 268 1524 Fragments arranged by size: 1524 1049 787 597 465 393 366 362 268 160 EcoRI G'AATT_C Cuts at: 3280 3658 3280 Size: 378 5593 FspI TGC'GCA Cuts at: 4477 4477 Size: 5971 HaeII r_GCGC'y Cuts at: 1678 5118 1678 Size: 3440 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3049 3081 4612 4644 4686 Size: 32 135 32 1477 32 1531 32 42 Cuts at: 4686 4718 1373 Size: 32 2626 Fragments arranged by size: 2626 1531 1477 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2211 2776 3315 3561 669 Size: 988 281 273 565 539 246 3079 Fragments arranged by size: 3079 988 565 539 281 273 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5971 HpaI GTT'AAC Cuts at: 3561 3561 Size: 5971 KpnI G_GTAC'C Cuts at: 3296 3296 Size: 5971 MfeI C'AATT_G Cuts at: 3570 3570 Size: 5971 MluI A'CGCG_T Cuts at: 5847 5847 Size: 5971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5085 5269 1305 Size: 3780 184 2007 Fragments arranged by size: 3780 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4149 Fragments arranged by size: 4149 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3929 4288 4447 518 Size: 648 2763 359 159 2042 Fragments arranged by size: 2763 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3387 4064 5212 5457 1606 Size: 1781 677 1148 245 2120 Fragments arranged by size: 2120 1781 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5419 NdeI CA'TA_TG Cuts at: 387 2743 387 Size: 2356 3615 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5971 NotI GC'GGCC_GC Cuts at: 3418 3418 Size: 5971 NspI r_CATG'y Cuts at: 1132 1229 2770 2899 3272 3329 4874 1132 Size: 97 1541 129 373 57 1545 2229 Fragments arranged by size: 2229 1545 1541 373 129 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5971 PciI A'CATG_T Cuts at: 2766 3268 4870 2766 Size: 502 1602 3867 Fragments arranged by size: 3867 1602 502 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5971 PmeI GTTT'AAAC Cuts at: 3275 3275 Size: 5971 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3077 3109 4040 4072 5553 5585 3077 Size: 32 931 32 1481 32 3463 Fragments arranged by size: 3463 1481 931 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5971 PsiI TTA'TAA Cuts at: 3541 3541 Size: 5971 PstI C_TGCA'G Cuts at: 830 2482 3323 830 Size: 1652 841 3478 Fragments arranged by size: 3478 1652 841 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5971 SalI G'TCGA_C Cuts at: 1936 3313 1936 Size: 1377 4594 SapI GCTCTTCn'nnn_ Cuts at: 2500 2500 Size: 5971 SbfI CC_TGCA'GG Cuts at: 3323 3323 Size: 5971 ScaI AGT'ACT Cuts at: 2094 4219 5931 2094 Size: 2125 1712 2134 Fragments arranged by size: 2134 2125 1712 SexAI A'CCwGG_T Cuts at: 2545 2545 Size: 5971 SfcI C'TryA_G Cuts at: 826 1045 2478 3319 4454 5135 5326 826 Size: 219 1433 841 1135 681 191 1471 Fragments arranged by size: 1471 1433 1135 841 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5971 SmaI CCC'GGG Cuts at: 1872 3298 1872 Size: 1426 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2581 4075 4976 5238 820 Size: 197 926 638 1494 901 262 1553 Fragments arranged by size: 1553 1494 926 901 638 262 197 SnaBI TAC'GTA Cuts at: 493 2673 3762 493 Size: 2180 1089 2702 Fragments arranged by size: 2702 2180 1089 SpeI A'CTAG_T Cuts at: 152 152 Size: 5971 SphI G_CATG'C Cuts at: 1132 3329 1132 Size: 2197 3774 SspI AAT'ATT Cuts at: 5 52 2568 2786 3895 5 Size: 47 2516 218 1109 2081 Fragments arranged by size: 2516 2081 1109 218 47 StuI AGG'CCT Cuts at: 1517 2281 2337 1517 Size: 764 56 5151 Fragments arranged by size: 5151 764 56 StyI C'CwwG_G Cuts at: 513 1065 3410 513 Size: 552 2345 3074 Fragments arranged by size: 3074 2345 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2196 2881 3998 4015 4168 4353 4798 Size: 1255 616 685 1117 17 153 185 445 Cuts at: 4798 325 Size: 1498 Fragments arranged by size: 1498 1255 1117 685 616 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2092 4217 5929 Size: 274 80 33 51 1443 115 2125 1712 Cuts at: 5929 96 Size: 138 Fragments arranged by size: 2125 1712 1443 274 138 115 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5931 5963 5931 Size: 32 5939 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5971 XbaI T'CTAG_A Cuts at: 3307 3307 Size: 5971 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5971 XmaI C'CCGG_G Cuts at: 1870 3296 1870 Size: 1426 4545 XmnI GAAnn'nnTTC Cuts at: 4100 4100 Size: 5971 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3779 276 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI