(Circular) (Six-base) MAPSORT of: pFN21AB5422.seq Check: 970 from: 1 to: 5377 pFN21AB5422 5377 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3187 278 Size: 53 83 186 936 1651 2468 Fragments arranged by size: 2468 1651 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2720 1937 Size: 783 4594 Acc65I G'GTAC_C Cuts at: 2698 2698 Size: 5377 AclI AA'CG_TT Cuts at: 1563 3504 3877 1563 Size: 1941 373 3063 Fragments arranged by size: 3063 1941 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2286 3440 4824 5092 1632 Size: 654 1154 1384 268 1917 Fragments arranged by size: 1917 1384 1154 654 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5180 AflIII A'CryG_T Cuts at: 1203 4276 5253 1203 Size: 3073 977 1327 Fragments arranged by size: 3073 1327 977 AgeI A'CCGG_T Cuts at: 4208 4208 Size: 5377 AhdI GACnn_n'nnGTC Cuts at: 4106 4106 Size: 5377 AleI CACnn'nnGTG Cuts at: 1166 2063 1166 Size: 897 4480 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2174 1184 Size: 990 4387 AlwNI CAG_nnn'CTG Cuts at: 2083 2112 2420 3120 4692 2083 Size: 29 308 700 1572 2768 Fragments arranged by size: 2768 1572 700 308 29 ApaI G_GGCC'C Cuts at: 2635 2635 Size: 5377 ApaLI G'TGCA_C Cuts at: 3434 4590 3434 Size: 1156 4221 ApoI r'AATT_y Cuts at: 1493 2351 2686 2912 3064 5173 1493 Size: 858 335 226 152 2109 1697 Fragments arranged by size: 2109 1697 858 335 226 152 AseI AT'TA_AT Cuts at: 160 1032 3931 160 Size: 872 2899 1606 Fragments arranged by size: 2899 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5377 AvaI C'yCGr_G Cuts at: 1870 1943 2033 2334 2600 2702 1870 Size: 73 90 301 266 102 4545 Fragments arranged by size: 4545 301 266 102 90 73 BamHI G'GATC_C Cuts at: 2707 3074 2707 Size: 367 5010 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2698 4153 618 Size: 325 217 627 911 1455 1842 Fragments arranged by size: 1842 1455 911 627 325 217 BanII G_rGCy'C Cuts at: 1391 2635 2661 2696 1391 Size: 1244 26 35 4072 Fragments arranged by size: 4072 1244 35 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4406 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2609 2643 3568 3602 1903 Size: 34 672 34 925 34 3678 Fragments arranged by size: 3678 925 672 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3269 4479 3269 Size: 1210 4167 BclI T'GATC_A Cuts at: 1548 1548 Size: 5377 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3988 136 Size: 107 122 71 631 605 2316 1525 Fragments arranged by size: 2316 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2211 5372 2211 Size: 3161 2216 BlpI GC'TnA_GC Cuts at: 2794 2794 Size: 5377 Bme1580I G_kGCm'C Cuts at: 1790 2070 2635 3438 4594 1790 Size: 280 565 803 1156 2573 Fragments arranged by size: 2573 1156 803 565 280 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5377 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2194 2591 3108 Size: 416 53 83 399 24 777 397 517 Cuts at: 3108 4066 442 Size: 958 1753 Fragments arranged by size: 1753 958 777 517 416 399 397 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5377 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2347 2347 Size: 5377 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2305 4037 1239 Size: 830 236 1732 2579 Fragments arranged by size: 2579 1732 830 236 Bpu10I CC'TnA_GC Cuts at: 2655 2655 Size: 5377 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3502 4367 4665 3502 Size: 865 298 4214 Fragments arranged by size: 4214 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2331 2510 4040 882 Size: 617 832 179 1530 2219 Fragments arranged by size: 2219 1530 832 617 179 BsaAI yAC'GTr Cuts at: 493 1204 2065 3168 493 Size: 711 861 1103 2702 Fragments arranged by size: 2702 1103 861 711 BsaBI GATnn'nnATC Cuts at: 5371 5371 Size: 5377 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2536 3184 3566 4201 Size: 53 83 186 936 1003 648 382 635 Cuts at: 4201 275 Size: 1451 Fragments arranged by size: 1451 1003 936 648 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3810 4208 4482 4629 5040 3810 Size: 398 274 147 411 4147 Fragments arranged by size: 4147 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2502 2532 1178 Size: 30 165 30 1099 30 4023 Fragments arranged by size: 4023 1099 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5377 BseYI C'CCAG_C Cuts at: 4580 5076 4580 Size: 496 4881 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2219 2234 1163 Size: 573 483 15 4306 Fragments arranged by size: 4306 573 483 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2827 3588 4616 1540 Size: 275 186 826 761 1028 2301 Fragments arranged by size: 2301 1028 826 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3438 3523 4594 3438 Size: 85 1071 4221 Fragments arranged by size: 4221 1071 85 BsmI GAATG_Cn' Cuts at: 2888 2981 2888 Size: 93 5284 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2635 2661 2696 3438 3523 4594 Size: 399 280 565 26 35 742 85 1071 Cuts at: 4594 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 565 399 280 85 35 26 BspEI T'CCGG_A Cuts at: 5040 5040 Size: 5377 BspHI T'CATG_A Cuts at: 3266 4996 3266 Size: 1730 3647 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2732 844 Size: 1888 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3264 1394 Size: 429 1441 3507 Fragments arranged by size: 3507 1441 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3872 4046 65 Size: 1183 2624 174 1396 Fragments arranged by size: 2624 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2586 4021 4208 1700 Size: 110 776 1435 187 2869 Fragments arranged by size: 2869 1435 776 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5377 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5377 BssSI C'ACGA_G Cuts at: 2132 3437 4449 2132 Size: 1305 1012 3060 Fragments arranged by size: 3060 1305 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5377 BstBI TT'CG_AA Cuts at: 4181 4181 Size: 5377 BstEII G'GTnAC_C Cuts at: 1184 2606 1184 Size: 1422 3955 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5377 BstYI r'GATC_y Cuts at: 1972 2211 2326 2707 3074 3459 3476 4928 5014 Size: 239 115 381 367 385 17 1452 86 Cuts at: 5014 5026 5372 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 381 367 346 239 115 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2196 513 Size: 552 939 192 3694 Fragments arranged by size: 3694 939 552 192 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2322 2500 487 Size: 683 56 1096 178 3364 Fragments arranged by size: 3364 1096 683 178 56 BtsI GCAGTG_nn' Cuts at: 2893 3687 3707 2893 Size: 794 20 4563 Fragments arranged by size: 4563 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5377 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5342 DraI TTT'AAA Cuts at: 2681 2681 Size: 5377 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4384 809 Size: 507 3068 1802 Fragments arranged by size: 3068 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2199 2824 3713 Size: 54 1425 50 275 18 369 625 889 Cuts at: 3713 5342 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 625 369 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2824 1537 Size: 275 1012 4090 Fragments arranged by size: 4090 1012 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2262 2475 3307 1353 Size: 909 213 832 3423 Fragments arranged by size: 3423 909 832 213 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3964 4338 4484 387 Size: 1064 138 2375 374 146 1280 Fragments arranged by size: 2375 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2286 2305 3440 4037 4824 5092 Size: 393 437 217 19 1135 597 787 268 Cuts at: 5092 1239 Size: 1524 Fragments arranged by size: 1524 1135 787 597 437 393 268 217 19 EcoNI CCTnn'n_nnAGG Cuts at: 2162 2162 Size: 5377 EcoO109I rG'GnC_Cy Cuts at: 2119 2446 2632 2119 Size: 327 186 4864 Fragments arranged by size: 4864 327 186 EcoRI G'AATT_C Cuts at: 2351 2686 3064 2351 Size: 335 378 4664 Fragments arranged by size: 4664 378 335 FspI TGC'GCA Cuts at: 3883 3883 Size: 5377 HaeII r_GCGC'y Cuts at: 1678 4524 1678 Size: 2846 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2376 2408 2648 2680 4018 Size: 32 135 32 804 32 240 32 1338 Cuts at: 4018 4050 4092 4124 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1338 804 240 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2721 2967 669 Size: 988 281 783 246 3079 Fragments arranged by size: 3079 988 783 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5377 HpaI GTT'AAC Cuts at: 2967 2967 Size: 5377 KpnI G_GTAC'C Cuts at: 2702 2702 Size: 5377 MfeI C'AATT_G Cuts at: 2976 2976 Size: 5377 MluI A'CGCG_T Cuts at: 5253 5253 Size: 5377 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2545 4491 4675 1305 Size: 1240 1946 184 2007 Fragments arranged by size: 2007 1946 1240 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3555 Fragments arranged by size: 3555 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2063 3335 3694 3853 518 Size: 648 843 54 1272 359 159 2042 Fragments arranged by size: 2042 1272 843 648 359 159 54 MspA1I CmG'CkG Cuts at: 1606 2080 2405 2639 2793 3470 4618 4863 1606 Size: 474 325 234 154 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 474 325 245 234 154 NaeI GCC'GGC Cuts at: 2588 2588 Size: 5377 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3886 Fragments arranged by size: 3886 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5377 NgoMIV G'CCGG_C Cuts at: 2586 2586 Size: 5377 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5377 NotI GC'GGCC_GC Cuts at: 2824 2824 Size: 5377 NspI r_CATG'y Cuts at: 1132 1229 2735 4280 1132 Size: 97 1506 1545 2229 Fragments arranged by size: 2229 1545 1506 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5377 PciI A'CATG_T Cuts at: 4276 4276 Size: 5377 PflMI CCAn_nnn'nTGG Cuts at: 1108 2197 1108 Size: 1089 4288 PmeI GTTT'AAAC Cuts at: 2681 2681 Size: 5377 PmlI CAC'GTG Cuts at: 2065 2065 Size: 5377 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3446 3478 4959 4991 3446 Size: 32 1481 32 3832 Fragments arranged by size: 3832 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2119 2446 2119 Size: 327 5050 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5377 PsiI TTA'TAA Cuts at: 2947 2947 Size: 5377 PspOMI G'GGCC_C Cuts at: 2631 2631 Size: 5377 PstI C_TGCA'G Cuts at: 830 2108 2729 830 Size: 1278 621 3478 Fragments arranged by size: 3478 1278 621 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5377 PvuII CAG'CTG Cuts at: 2080 2405 2080 Size: 325 5052 SalI G'TCGA_C Cuts at: 1936 2719 1936 Size: 783 4594 SapI GCTCTTCn'nnn_ Cuts at: 2475 2475 Size: 5377 SbfI CC_TGCA'GG Cuts at: 2729 2729 Size: 5377 ScaI AGT'ACT Cuts at: 3625 5337 3625 Size: 1712 3665 SexAI A'CCwGG_T Cuts at: 2364 2364 Size: 5377 SfcI C'TryA_G Cuts at: 826 1045 2104 2725 3860 4541 4732 826 Size: 219 1059 621 1135 681 191 1471 Fragments arranged by size: 1471 1135 1059 681 621 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5377 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5377 SmaI CCC'GGG Cuts at: 1872 2704 1872 Size: 832 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2334 3481 4382 4644 820 Size: 197 926 391 1147 901 262 1553 Fragments arranged by size: 1553 1147 926 901 391 262 197 SnaBI TAC'GTA Cuts at: 493 3168 493 Size: 2675 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5377 SphI G_CATG'C Cuts at: 1132 2735 1132 Size: 1603 3774 SspI AAT'ATT Cuts at: 5 52 3301 5 Size: 47 3249 2081 Fragments arranged by size: 3249 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5377 StyI C'CwwG_G Cuts at: 513 1065 2004 2816 513 Size: 552 939 812 3074 Fragments arranged by size: 3074 939 812 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3404 3421 3574 3759 4204 325 Size: 1255 1824 17 153 185 445 1498 Fragments arranged by size: 1824 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3623 5335 96 Size: 274 80 33 51 1443 1646 1712 138 Fragments arranged by size: 1712 1646 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2502 2534 5337 5369 2502 Size: 32 2803 32 2510 Fragments arranged by size: 2803 2510 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5377 XbaI T'CTAG_A Cuts at: 2713 2713 Size: 5377 XhoI C'TCGA_G Cuts at: 1943 2334 1943 Size: 391 4986 XmaI C'CCGG_G Cuts at: 1870 2702 1870 Size: 832 4545 XmnI GAAnn'nnTTC Cuts at: 2213 2351 3506 2213 Size: 138 1155 4084 Fragments arranged by size: 4084 1155 138 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3185 276 Size: 53 83 186 936 1651 2468 Fragments arranged by size: 2468 1651 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI