Restriction map for
pFN21AB5272
(Linear) (Six-base) MAPSORT of: KIBB5272.seq Check: 5980 from: 1 to: 744
KIBB5272.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 744
Size: 502 242
AlwNI CAG_nnn'CTG
Cuts at: 0 48 723 744
Size: 48 675 21
Fragments arranged by size:
675 48 21
ApaI G_GGCC'C
Cuts at: 0 123 744
Size: 123 621
AvaI C'yCGr_G
Cuts at: 0 122 744
Size: 122 622
BamHI G'GATC_C
Cuts at: 0 164 744
Size: 164 580
BanII G_rGCy'C
Cuts at: 0 123 744
Size: 123 621
BbvCI CC'TCA_GC
Cuts at: 0 134 140 744
Size: 134 6 604
Fragments arranged by size:
604 134 6
BclI T'GATC_A
Cuts at: 0 401 744
Size: 401 343
BlpI GC'TnA_GC
Cuts at: 0 83 344 440 744
Size: 83 261 96 304
Fragments arranged by size:
304 261 96 83
Bme1580I G_kGCm'C
Cuts at: 0 123 744
Size: 123 621
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 165 549 744
Size: 165 384 195
Fragments arranged by size:
384 195 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 304 490 744
Size: 76 228 186 254
Fragments arranged by size:
254 228 186 76
Bpu10I CC'TnA_GC
Cuts at: 0 134 140 197 518 744
Size: 134 6 57 321 226
Fragments arranged by size:
321 226 134 57 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 532 571 744
Size: 487 45 39 173
Fragments arranged by size:
487 173 45 39
BsaI GGTCTCn'nnnn_
Cuts at: 0 592 744
Size: 592 152
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 487 490 700 744
Size: 88 399 3 210 44
Fragments arranged by size:
399 210 88 44 3
BseYI C'CCAG_C
Cuts at: 0 4 744
Size: 4 740
BsiHKAI G_wGCw'C
Cuts at: 0 198 692 744
Size: 198 494 52
Fragments arranged by size:
494 198 52
Bsp1286I G_dGCh'C
Cuts at: 0 123 198 692 744
Size: 123 75 494 52
Fragments arranged by size:
494 123 75 52
BsrDI GCAATG_nn'
Cuts at: 0 350 744
Size: 350 394
BsrGI T'GTAC_A
Cuts at: 0 697 744
Size: 697 47
BstAPI GCAn_nnn'nTGC
Cuts at: 0 67 744
Size: 67 677
BstYI r'GATC_y
Cuts at: 0 38 164 744
Size: 38 126 580
Fragments arranged by size:
580 126 38
BtsI GCAGTG_nn'
Cuts at: 0 212 744
Size: 212 532
EaeI y'GGCC_r
Cuts at: 0 209 744
Size: 209 535
EarI CTCTTCn'nnn_
Cuts at: 0 176 353 374 744
Size: 176 177 21 370
Fragments arranged by size:
370 177 176 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 538 744
Size: 538 206
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 304 490 502 744
Size: 76 228 186 12 242
Fragments arranged by size:
242 228 186 76 12
HaeII r_GCGC'y
Cuts at: 0 90 207 744
Size: 90 117 537
Fragments arranged by size:
537 117 90
MfeI C'AATT_G
Cuts at: 0 647 744
Size: 647 97
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 744
Size: 354 390
MscI TGG'CCA
Cuts at: 0 211 744
Size: 211 533
MspA1I CmG'CkG
Cuts at: 0 171 744
Size: 171 573
PfoI T'CCnGG_A
Cuts at: 0 320 744
Size: 320 424
PspOMI G'GGCC_C
Cuts at: 0 119 744
Size: 119 625
PstI C_TGCA'G
Cuts at: 0 53 744
Size: 53 691
PvuII CAG'CTG
Cuts at: 0 171 744
Size: 171 573
SapI GCTCTTCn'nnn_
Cuts at: 0 176 744
Size: 176 568
ScaI AGT'ACT
Cuts at: 0 532 744
Size: 532 212
SfcI C'TryA_G
Cuts at: 0 49 744
Size: 49 695
SmaI CCC'GGG
Cuts at: 0 124 744
Size: 124 620
SmlI C'TyrA_G
Cuts at: 0 502 511 586 744
Size: 502 9 75 158
Fragments arranged by size:
502 158 75 9
TatI w'GTAC_w
Cuts at: 0 530 697 744
Size: 530 167 47
Fragments arranged by size:
530 167 47
XmaI C'CCGG_G
Cuts at: 0 122 744
Size: 122 622
Enzymes that do cut:
AcuI AlwNI ApaI AvaI BamHI BanII BbvCI BclI
BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BseRI
BseYI BsiHKAI Bsp1286I BsrDI BsrGI BstAPI BstYI BtsI
EaeI EarI EciI Eco57MI HaeII MfeI MmeI MscI
MspA1I PfoI PspOMI PstI PvuII SapI ScaI SfcI
SmaI SmlI TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BanI BbeI BbsI BcgI
BciVI BfrBI BglI BglII BmgBI BmrI BmtI BsaAI
BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MluI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmnI ZraI