(Circular) (Six-base) MAPSORT of: pFN21AE2267.seq Check: 5441 from: 1 to: 7549 pFN21AE2267 7549 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5359 278 Size: 53 83 186 936 3823 2468 Fragments arranged by size: 3823 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3714 4892 1937 Size: 1777 1178 4594 Fragments arranged by size: 4594 1777 1178 Acc65I G'GTAC_C Cuts at: 4870 4870 Size: 7549 AclI AA'CG_TT Cuts at: 1563 5676 6049 1563 Size: 4113 373 3063 Fragments arranged by size: 4113 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2534 3500 4011 5612 6996 7264 1632 Size: 902 966 511 1601 1384 268 1917 Fragments arranged by size: 1917 1601 1384 966 902 511 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7352 AflIII A'CryG_T Cuts at: 1203 2731 2733 4221 4766 6448 7425 1203 Size: 1528 2 1488 545 1682 977 1327 Fragments arranged by size: 1682 1528 1488 1327 977 545 2 AgeI A'CCGG_T Cuts at: 6380 6380 Size: 7549 AhdI GACnn_n'nnGTC Cuts at: 6278 6278 Size: 7549 AleI CACnn'nnGTG Cuts at: 1166 4755 1166 Size: 3589 3960 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7549 AlwNI CAG_nnn'CTG Cuts at: 3998 4760 5292 6864 3998 Size: 762 532 1572 4683 Fragments arranged by size: 4683 1572 762 532 ApaI G_GGCC'C Cuts at: 3193 3676 3193 Size: 483 7066 ApaLI G'TGCA_C Cuts at: 5606 6762 5606 Size: 1156 6393 ApoI r'AATT_y Cuts at: 1493 3440 3773 4666 4858 5084 5236 7345 1493 Size: 1947 333 893 192 226 152 2109 1697 Fragments arranged by size: 2109 1947 1697 893 333 226 192 152 AseI AT'TA_AT Cuts at: 160 1032 6103 160 Size: 872 5071 1606 Fragments arranged by size: 5071 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7549 AvaI C'yCGr_G Cuts at: 1870 1943 2137 2516 3280 3554 4874 1870 Size: 73 194 379 764 274 1320 4545 Fragments arranged by size: 4545 1320 764 379 274 194 73 BamHI G'GATC_C Cuts at: 2120 3733 4879 5246 2120 Size: 1613 1146 367 4423 Fragments arranged by size: 4423 1613 1146 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4340 4439 4870 6325 618 Size: 325 217 627 2553 99 431 1455 1842 Fragments arranged by size: 2553 1842 1455 627 431 325 217 99 BanII G_rGCy'C Cuts at: 1391 2138 3193 3676 4641 4868 1391 Size: 747 1055 483 965 227 4072 Fragments arranged by size: 4072 1055 965 747 483 227 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3912 4216 4821 928 Size: 971 2013 304 605 3656 Fragments arranged by size: 3656 2013 971 605 304 BbvCI CC'TCA_GC Cuts at: 4559 4559 Size: 7549 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5740 5774 1903 Size: 34 3803 34 3678 Fragments arranged by size: 3803 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5441 6651 5441 Size: 1210 6339 BclI T'GATC_A Cuts at: 1548 1548 Size: 7549 BfrBI ATG'CAT Cuts at: 3584 3584 Size: 7549 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6160 136 Size: 107 122 71 631 605 4488 1525 Fragments arranged by size: 4488 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4254 7544 4254 Size: 3290 4259 BlpI GC'TnA_GC Cuts at: 4966 4966 Size: 7549 Bme1580I G_kGCm'C Cuts at: 1790 3193 3676 5610 6766 1790 Size: 1403 483 1934 1156 2573 Fragments arranged by size: 2573 1934 1403 1156 483 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7549 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3085 5280 6238 Size: 416 53 83 399 24 1668 2195 958 Cuts at: 6238 442 Size: 1753 Fragments arranged by size: 2195 1753 1668 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7549 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2954 2954 Size: 7549 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3230 3551 3841 6209 1239 Size: 1991 321 290 2368 2579 Fragments arranged by size: 2579 2368 1991 321 290 Bpu10I CC'TnA_GC Cuts at: 4559 4559 Size: 7549 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2907 5674 6539 6837 2907 Size: 2767 865 298 3619 Fragments arranged by size: 3619 2767 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4015 6212 882 Size: 617 2516 2197 2219 Fragments arranged by size: 2516 2219 2197 617 BsaAI yAC'GTr Cuts at: 493 1204 2734 5340 493 Size: 711 1530 2606 2702 Fragments arranged by size: 2702 2606 1530 711 BsaBI GATnn'nnATC Cuts at: 7543 7543 Size: 7549 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2708 4213 5356 5738 Size: 53 83 186 936 1175 1505 1143 382 Cuts at: 5738 6373 275 Size: 635 1451 Fragments arranged by size: 1505 1451 1175 1143 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5982 6380 6654 6801 7212 5982 Size: 398 274 147 411 6319 Fragments arranged by size: 6319 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3071 3101 1178 Size: 30 165 30 1668 30 5626 Fragments arranged by size: 5626 1668 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2198 4609 1186 Size: 1012 2411 4126 Fragments arranged by size: 4126 2411 1012 BseYI C'CCAG_C Cuts at: 2310 6752 7248 2310 Size: 4442 496 2611 Fragments arranged by size: 4442 2611 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2083 2663 4619 1163 Size: 573 347 580 1956 4093 Fragments arranged by size: 4093 1956 580 573 347 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2078 3070 4999 5760 6788 1540 Size: 275 186 77 992 1929 761 1028 2301 Fragments arranged by size: 2301 1929 1028 992 761 275 186 77 BsiHKAI G_wGCw'C Cuts at: 2970 2985 3972 5610 5695 6766 2970 Size: 15 987 1638 85 1071 3753 Fragments arranged by size: 3753 1638 1071 987 85 15 BsmI GAATG_Cn' Cuts at: 2693 4614 5060 5153 2693 Size: 1921 446 93 5089 Fragments arranged by size: 5089 1921 446 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2534 2534 Size: 7549 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2138 2970 2985 3193 3676 3972 4641 Size: 399 348 832 15 208 483 296 669 Cuts at: 4641 4868 5610 5695 6766 1391 Size: 227 742 85 1071 2174 Fragments arranged by size: 2174 1071 832 742 669 483 399 348 296 227 208 85 15 BspEI T'CCGG_A Cuts at: 7212 7212 Size: 7549 BspHI T'CATG_A Cuts at: 5438 7168 5438 Size: 1730 5819 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2055 4904 844 Size: 1211 2849 3489 Fragments arranged by size: 3489 2849 1211 BsrBI CCG'CTC Cuts at: 1394 1823 5436 1394 Size: 429 3613 3507 Fragments arranged by size: 3613 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2949 4024 6044 6218 65 Size: 1183 1701 1075 2020 174 1396 Fragments arranged by size: 2020 1701 1396 1183 1075 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6193 6380 1700 Size: 110 4383 187 2869 Fragments arranged by size: 4383 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7549 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7549 BssSI C'ACGA_G Cuts at: 3617 3965 5609 6621 3617 Size: 348 1644 1012 4545 Fragments arranged by size: 4545 1644 1012 348 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7549 BstBI TT'CG_AA Cuts at: 6353 6353 Size: 7549 BstEII G'GTnAC_C Cuts at: 1184 2585 1184 Size: 1401 6148 BstYI r'GATC_y Cuts at: 1972 2120 3169 3733 4022 4254 4879 5246 5631 Size: 148 1049 564 289 232 625 367 385 Cuts at: 5631 5648 7100 7186 7198 7544 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1049 625 564 385 367 346 289 232 148 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2029 2078 3107 513 Size: 552 964 49 1029 4955 Fragments arranged by size: 4955 1029 964 552 49 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6810 Fragments arranged by size: 6810 683 56 BtsI GCAGTG_nn' Cuts at: 2066 2985 3014 4527 5065 5859 5879 2066 Size: 919 29 1513 538 794 20 3736 Fragments arranged by size: 3736 1513 919 794 538 29 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7549 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7514 DraI TTT'AAA Cuts at: 3424 3838 4410 4853 3424 Size: 414 572 443 6120 Fragments arranged by size: 6120 572 443 414 DraIII CAC_nnn'GTG Cuts at: 2974 2974 Size: 7549 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6556 809 Size: 507 5240 1802 Fragments arranged by size: 5240 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2075 3063 4368 Size: 54 1425 50 275 18 245 988 1305 Cuts at: 4368 4479 4996 5885 7514 8 Size: 111 517 889 1629 43 Fragments arranged by size: 1629 1425 1305 988 889 517 275 245 111 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2075 4996 1537 Size: 275 263 2921 4090 Fragments arranged by size: 4090 2921 275 263 EarI CTCTTCn'nnn_ Cuts at: 1353 3280 3566 3888 5479 1353 Size: 1927 286 322 1591 3423 Fragments arranged by size: 3423 1927 1591 322 286 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2807 2993 6136 6510 6656 387 Size: 1064 138 1218 186 3143 374 146 1280 Fragments arranged by size: 3143 1280 1218 1064 374 186 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2534 3230 3500 3551 3841 4011 5612 Size: 393 902 696 270 51 290 170 1601 Cuts at: 5612 6209 6996 7264 1239 Size: 597 787 268 1524 Fragments arranged by size: 1601 1524 902 787 696 597 393 290 270 268 170 51 EcoNI CCTnn'n_nnAGG Cuts at: 3197 3197 Size: 7549 EcoO109I rG'GnC_Cy Cuts at: 2067 3189 3190 3672 4468 4711 2067 Size: 1122 1 482 796 243 4905 Fragments arranged by size: 4905 1122 796 482 243 1 EcoRI G'AATT_C Cuts at: 3440 4858 5236 3440 Size: 1418 378 5753 Fragments arranged by size: 5753 1418 378 FspI TGC'GCA Cuts at: 6055 6055 Size: 7549 HaeII r_GCGC'y Cuts at: 1678 6696 1678 Size: 5018 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2291 2323 3796 3828 4713 Size: 32 135 32 719 32 1473 32 885 Cuts at: 4713 4745 6190 6222 6264 6296 1373 Size: 32 1445 32 42 32 2626 Fragments arranged by size: 2626 1473 1445 885 719 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3520 4893 5139 669 Size: 988 281 1582 1373 246 3079 Fragments arranged by size: 3079 1582 1373 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7549 HpaI GTT'AAC Cuts at: 5139 5139 Size: 7549 KpnI G_GTAC'C Cuts at: 4874 4874 Size: 7549 MfeI C'AATT_G Cuts at: 5148 5148 Size: 7549 MluI A'CGCG_T Cuts at: 7425 7425 Size: 7549 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2919 3054 3465 6663 6847 1305 Size: 1614 135 411 3198 184 2007 Fragments arranged by size: 3198 2007 1614 411 184 135 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5727 Fragments arranged by size: 5727 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4226 4239 4487 4595 4755 4771 5507 Size: 648 3060 13 248 108 160 16 736 Cuts at: 5507 5866 6025 518 Size: 359 159 2042 Fragments arranged by size: 3060 2042 736 648 359 248 160 159 108 16 13 MspA1I CmG'CkG Cuts at: 1606 2031 2080 3075 4965 5642 6790 7035 1606 Size: 425 49 995 1890 677 1148 245 2120 Fragments arranged by size: 2120 1890 1148 995 677 425 245 49 NcoI C'CATG_G Cuts at: 513 1065 3107 513 Size: 552 2042 4955 Fragments arranged by size: 4955 2042 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7549 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7549 NotI GC'GGCC_GC Cuts at: 2075 4996 2075 Size: 2921 4628 NsiI A_TGCA'T Cuts at: 3586 3586 Size: 7549 NspI r_CATG'y Cuts at: 1132 1229 2046 3004 4770 4907 6452 1132 Size: 97 817 958 1766 137 1545 2229 Fragments arranged by size: 2229 1766 1545 958 817 137 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7549 PciI A'CATG_T Cuts at: 4766 6448 4766 Size: 1682 5867 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7549 PfoI T'CCnGG_A Cuts at: 2725 3154 3165 3729 2725 Size: 429 11 564 6545 Fragments arranged by size: 6545 564 429 11 PmeI GTTT'AAAC Cuts at: 4853 4853 Size: 7549 PmlI CAC'GTG Cuts at: 2734 2734 Size: 7549 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5618 5650 7131 7163 5618 Size: 32 1481 32 6004 Fragments arranged by size: 6004 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2067 4468 4711 2067 Size: 2401 243 4905 Fragments arranged by size: 4905 2401 243 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7549 PsiI TTA'TAA Cuts at: 3864 5119 3864 Size: 1255 6294 PspOMI G'GGCC_C Cuts at: 3189 3672 3189 Size: 483 7066 PstI C_TGCA'G Cuts at: 830 3009 3339 3960 4330 4901 830 Size: 2179 330 621 370 571 3478 Fragments arranged by size: 3478 2179 621 571 370 330 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7549 PvuII CAG'CTG Cuts at: 3075 3075 Size: 7549 SacII CC_GC'GG Cuts at: 2032 2081 2032 Size: 49 7500 SalI G'TCGA_C Cuts at: 1936 4891 1936 Size: 2955 4594 SapI GCTCTTCn'nnn_ Cuts at: 3566 3566 Size: 7549 SbfI CC_TGCA'GG Cuts at: 4901 4901 Size: 7549 ScaI AGT'ACT Cuts at: 2189 5797 7509 2189 Size: 3608 1712 2229 Fragments arranged by size: 3608 2229 1712 SfcI C'TryA_G Cuts at: 826 1045 3005 3335 3956 4299 4326 4897 6032 Size: 219 1960 330 621 343 27 571 1135 Cuts at: 6032 6713 6904 826 Size: 681 191 1471 Fragments arranged by size: 1960 1471 1135 681 621 571 343 330 219 191 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7549 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7549 SmaI CCC'GGG Cuts at: 1872 2139 2518 3282 4876 1872 Size: 267 379 764 1594 4545 Fragments arranged by size: 4545 1594 764 379 267 SmlI C'TyrA_G Cuts at: 820 1017 1943 2922 5653 6554 6816 820 Size: 197 926 979 2731 901 262 1553 Fragments arranged by size: 2731 1553 979 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5340 493 Size: 4847 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7549 SphI G_CATG'C Cuts at: 1132 2046 4907 1132 Size: 914 2861 3774 Fragments arranged by size: 3774 2861 914 SrfI GCCC'GGGC Cuts at: 2139 2139 Size: 7549 SspI AAT'ATT Cuts at: 5 52 3636 4462 5473 5 Size: 47 3584 826 1011 2081 Fragments arranged by size: 3584 2081 1011 826 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7549 StyI C'CwwG_G Cuts at: 513 1065 3107 3539 4717 4988 513 Size: 552 2042 432 1178 271 3074 Fragments arranged by size: 3074 2042 1178 552 432 271 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2573 3929 5576 5593 5746 5931 6376 Size: 1255 993 1356 1647 17 153 185 445 Cuts at: 6376 325 Size: 1498 Fragments arranged by size: 1647 1498 1356 1255 993 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2187 3289 5795 Size: 274 80 33 51 1443 210 1102 2506 Cuts at: 5795 7507 96 Size: 1712 138 Fragments arranged by size: 2506 1712 1443 1102 274 210 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2549 2581 3260 3292 4507 4539 7509 7541 2549 Size: 32 679 32 1215 32 2970 32 2557 Fragments arranged by size: 2970 2557 1215 679 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7549 XbaI T'CTAG_A Cuts at: 4885 4885 Size: 7549 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7549 XmaI C'CCGG_G Cuts at: 1870 2137 2516 3280 4874 1870 Size: 267 379 764 1594 4545 Fragments arranged by size: 4545 1594 764 379 267 XmnI GAAnn'nnTTC Cuts at: 3612 5678 3612 Size: 2066 5483 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5357 276 Size: 53 83 186 936 3823 2468 Fragments arranged by size: 3823 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SwaI XcmI