(Circular) (Six-base) MAPSORT of: pFN21AE2122.seq Check: 3032 from: 1 to: 7420 pFN21AE2122 7420 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4051 4051 Size: 7420 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3456 5230 278 Size: 53 83 186 936 1920 1774 2468 Fragments arranged by size: 2468 1920 1774 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4763 1937 Size: 2826 4594 Acc65I G'GTAC_C Cuts at: 4741 4741 Size: 7420 AclI AA'CG_TT Cuts at: 1563 3801 5547 5920 1563 Size: 2238 1746 373 3063 Fragments arranged by size: 3063 2238 1746 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2615 2663 4647 5483 6867 7135 1632 Size: 983 48 1984 836 1384 268 1917 Fragments arranged by size: 1984 1917 1384 983 836 268 48 AfeI AGC'GCT Cuts at: 2949 2949 Size: 7420 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7223 AflIII A'CryG_T Cuts at: 1203 6319 7296 1203 Size: 5116 977 1327 Fragments arranged by size: 5116 1327 977 AgeI A'CCGG_T Cuts at: 6251 6251 Size: 7420 AhdI GACnn_n'nnGTC Cuts at: 6149 6149 Size: 7420 AleI CACnn'nnGTG Cuts at: 1166 3518 1166 Size: 2352 5068 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2535 3032 1184 Size: 1351 497 5572 Fragments arranged by size: 5572 1351 497 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3812 3844 3812 Size: 32 7388 AlwNI CAG_nnn'CTG Cuts at: 2593 3037 4408 4553 5163 6735 2593 Size: 444 1371 145 610 1572 3278 Fragments arranged by size: 3278 1572 1371 610 444 145 ApaLI G'TGCA_C Cuts at: 5477 6633 5477 Size: 1156 6264 ApoI r'AATT_y Cuts at: 1493 2212 4631 4697 4729 4955 5107 7216 1493 Size: 719 2419 66 32 226 152 2109 1697 Fragments arranged by size: 2419 2109 1697 719 226 152 66 32 AseI AT'TA_AT Cuts at: 160 1032 4230 5974 160 Size: 872 3198 1744 1606 Fragments arranged by size: 3198 1744 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7420 AvaI C'yCGr_G Cuts at: 1870 1943 2631 3648 4745 1870 Size: 73 688 1017 1097 4545 Fragments arranged by size: 4545 1097 1017 688 73 BamHI G'GATC_C Cuts at: 2599 4750 5117 2599 Size: 2151 367 4902 Fragments arranged by size: 4902 2151 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2366 4741 6196 618 Size: 325 217 627 579 2375 1455 1842 Fragments arranged by size: 2375 1842 1455 627 579 325 217 BanII G_rGCy'C Cuts at: 1391 2641 4422 4739 1391 Size: 1250 1781 317 4072 Fragments arranged by size: 4072 1781 1250 317 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2587 3448 928 Size: 971 688 861 4900 Fragments arranged by size: 4900 971 861 688 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2816 2850 5611 5645 1903 Size: 34 879 34 2761 34 3678 Fragments arranged by size: 3678 2761 879 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5312 6522 5312 Size: 1210 6210 BclI T'GATC_A Cuts at: 1548 3891 4164 4294 1548 Size: 2343 273 130 4674 Fragments arranged by size: 4674 2343 273 130 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2636 2817 3305 Size: 107 122 71 631 605 964 181 488 Cuts at: 3305 4019 6031 136 Size: 714 2012 1525 Fragments arranged by size: 2012 1525 964 714 631 605 488 181 122 107 71 BglII A'GATC_T Cuts at: 2254 3127 7415 2254 Size: 873 4288 2259 Fragments arranged by size: 4288 2259 873 BlpI GC'TnA_GC Cuts at: 3041 4056 4837 3041 Size: 1015 781 5624 Fragments arranged by size: 5624 1015 781 Bme1580I G_kGCm'C Cuts at: 1790 5481 6637 1790 Size: 3691 1156 2573 Fragments arranged by size: 3691 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7420 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5151 6109 442 Size: 416 53 83 399 24 3734 958 1753 Fragments arranged by size: 3734 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7420 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3104 4436 3104 Size: 1332 6088 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2933 2945 3014 4575 6080 1239 Size: 1694 12 69 1561 1505 2579 Fragments arranged by size: 2579 1694 1561 1505 69 12 Bpu10I CC'TnA_GC Cuts at: 2241 3438 2241 Size: 1197 6223 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2661 2763 5545 6410 6708 2661 Size: 102 2782 865 298 3373 Fragments arranged by size: 3373 2782 865 298 102 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6083 882 Size: 617 4584 2219 Fragments arranged by size: 4584 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5211 493 Size: 711 4007 2702 Fragments arranged by size: 4007 2702 711 BsaBI GATnn'nnATC Cuts at: 2315 7414 2315 Size: 5099 2321 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3453 5227 5609 6244 Size: 53 83 186 936 1920 1774 382 635 Cuts at: 6244 275 Size: 1451 Fragments arranged by size: 1920 1774 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2061 3615 5853 6251 6525 6672 7083 2061 Size: 1554 2238 398 274 147 411 2398 Fragments arranged by size: 2398 2238 1554 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3812 3842 1178 Size: 30 165 30 2409 30 4756 Fragments arranged by size: 4756 2409 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2314 2930 2953 3833 4052 4428 1186 Size: 1128 616 23 880 219 376 4178 Fragments arranged by size: 4178 1128 880 616 376 219 23 BseYI C'CCAG_C Cuts at: 3692 6623 7119 3692 Size: 2931 496 3993 Fragments arranged by size: 3993 2931 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2953 4421 1163 Size: 573 1217 1468 4162 Fragments arranged by size: 4162 1468 1217 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2906 2983 4870 5631 6659 1540 Size: 275 186 905 77 1887 761 1028 2301 Fragments arranged by size: 2301 1887 1028 905 761 275 186 77 BsiHKAI G_wGCw'C Cuts at: 4422 5481 5566 6637 4422 Size: 1059 85 1071 5205 Fragments arranged by size: 5205 1071 1059 85 BsmI GAATG_Cn' Cuts at: 2656 4486 4931 5024 2656 Size: 1830 445 93 5052 Fragments arranged by size: 5052 1830 445 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2300 2970 2300 Size: 670 6750 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2641 4422 4739 5481 5566 6637 1391 Size: 399 851 1781 317 742 85 1071 2174 Fragments arranged by size: 2174 1781 1071 851 742 399 317 85 BspEI T'CCGG_A Cuts at: 2061 7083 2061 Size: 5022 2398 BspHI T'CATG_A Cuts at: 2442 4167 5309 7039 2442 Size: 1725 1142 1730 2823 Fragments arranged by size: 2823 1730 1725 1142 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3045 3469 4051 4247 4775 844 Size: 2201 424 582 196 528 3489 Fragments arranged by size: 3489 2201 582 528 424 196 BsrBI CCG'CTC Cuts at: 1394 1823 3228 3401 5307 1394 Size: 429 1405 173 1906 3507 Fragments arranged by size: 3507 1906 1405 429 173 BsrDI GCAATG_nn' Cuts at: 65 1248 3093 3990 4135 4713 5915 6089 65 Size: 1183 1845 897 145 578 1202 174 1396 Fragments arranged by size: 1845 1396 1202 1183 897 578 174 145 BsrFI r'CCGG_y Cuts at: 1700 1810 2759 2982 6064 6251 1700 Size: 110 949 223 3082 187 2869 Fragments arranged by size: 3082 2869 949 223 187 110 BsrGI T'GTAC_A Cuts at: 96 2664 4681 96 Size: 2568 2017 2835 Fragments arranged by size: 2835 2568 2017 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7420 BssSI C'ACGA_G Cuts at: 4281 4641 5480 6492 4281 Size: 360 839 1012 5209 Fragments arranged by size: 5209 1012 839 360 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7420 BstBI TT'CG_AA Cuts at: 6224 6224 Size: 7420 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7420 BstXI CCAn_nnnn'nTGG Cuts at: 2053 3286 2053 Size: 1233 6187 BstYI r'GATC_y Cuts at: 1972 2254 2599 3082 3127 4750 5117 5502 5519 Size: 282 345 483 45 1623 367 385 17 Cuts at: 5519 6971 7057 7069 7415 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1623 1452 483 385 367 346 345 282 86 45 17 12 BtgI C'CryG_G Cuts at: 513 1065 2370 2400 3501 513 Size: 552 1305 30 1101 4432 Fragments arranged by size: 4432 1305 1101 552 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2740 3612 4434 487 Size: 683 56 1514 872 822 3473 Fragments arranged by size: 3473 1514 872 822 683 56 BtsI GCAGTG_nn' Cuts at: 3159 4936 5730 5750 3159 Size: 1777 794 20 4829 Fragments arranged by size: 4829 1777 794 20 ClaI AT'CG_AT Cuts at: 1554 3834 1554 Size: 2280 5140 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3349 3384 529 Size: 35 2785 35 4565 Fragments arranged by size: 4565 2785 35 35 DraI TTT'AAA Cuts at: 2533 4724 2533 Size: 2191 5229 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6427 809 Size: 507 5111 1802 Fragments arranged by size: 5111 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2903 2955 3419 Size: 54 1425 50 275 18 1073 52 464 Cuts at: 3419 4107 4146 4867 5756 7385 8 Size: 688 39 721 889 1629 43 Fragments arranged by size: 1629 1425 1073 889 721 688 464 275 54 52 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 2903 4867 1537 Size: 275 1091 1964 4090 Fragments arranged by size: 4090 1964 1091 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2259 2469 4607 5350 1353 Size: 906 210 2138 743 3423 Fragments arranged by size: 3423 2138 906 743 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2441 2678 3017 6007 6381 6527 Size: 1064 138 852 237 339 2990 374 146 Cuts at: 6527 387 Size: 1280 Fragments arranged by size: 2990 1280 1064 852 374 339 237 146 138 EcoICRI GAG'CTC Cuts at: 4420 4420 Size: 7420 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2615 2663 2933 2945 3014 4575 4647 Size: 393 983 48 270 12 69 1561 72 Cuts at: 4647 5483 6080 6867 7135 1239 Size: 836 597 787 268 1524 Fragments arranged by size: 1561 1524 983 836 787 597 393 270 268 72 69 48 12 EcoNI CCTnn'n_nnAGG Cuts at: 2222 3590 2222 Size: 1368 6052 EcoO109I rG'GnC_Cy Cuts at: 2041 2479 3034 3897 2041 Size: 438 555 863 5564 Fragments arranged by size: 5564 863 555 438 EcoRI G'AATT_C Cuts at: 4631 4697 4729 5107 4631 Size: 66 32 378 6944 Fragments arranged by size: 6944 378 66 32 EcoRV GAT'ATC Cuts at: 4135 4135 Size: 7420 FspI TGC'GCA Cuts at: 2294 5926 2294 Size: 3632 3788 HaeII r_GCGC'y Cuts at: 1678 2951 3029 6567 1678 Size: 1273 78 3538 2531 Fragments arranged by size: 3538 2531 1273 78 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2239 2271 2333 2365 3152 Size: 32 135 32 667 32 62 32 787 Cuts at: 3152 3184 3500 3518 3532 3550 4060 4092 4573 Size: 32 316 18 14 18 510 32 481 Cuts at: 4573 4605 6061 6093 6135 6167 1373 Size: 32 1456 32 42 32 2626 Fragments arranged by size: 2626 1456 787 667 510 481 316 135 62 42 32 32 32 32 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 2122 3492 4558 4764 5010 669 Size: 988 281 184 1370 1066 206 246 3079 Fragments arranged by size: 3079 1370 1066 988 281 246 206 184 HindIII A'AGCT_T Cuts at: 748 748 Size: 7420 HpaI GTT'AAC Cuts at: 5010 5010 Size: 7420 KpnI G_GTAC'C Cuts at: 4745 4745 Size: 7420 MfeI C'AATT_G Cuts at: 5019 5019 Size: 7420 MluI A'CGCG_T Cuts at: 7296 7296 Size: 7420 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2067 3345 3696 6534 6718 1305 Size: 762 1278 351 2838 184 2007 Fragments arranged by size: 2838 2007 1278 762 351 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4109 4148 10 Size: 54 1425 343 2277 39 3282 Fragments arranged by size: 3282 2277 1425 343 54 39 MslI CAynn'nnrTG Cuts at: 518 1166 2697 3518 5378 5737 5896 518 Size: 648 1531 821 1860 359 159 2042 Fragments arranged by size: 2042 1860 1531 821 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2402 2940 4125 4553 4836 5513 6661 6906 Size: 796 538 1185 428 283 677 1148 245 Cuts at: 6906 1606 Size: 2120 Fragments arranged by size: 2120 1185 1148 796 677 538 428 283 245 NaeI GCC'GGC Cuts at: 2761 2761 Size: 7420 NcoI C'CATG_G Cuts at: 513 1065 2370 513 Size: 552 1305 5563 Fragments arranged by size: 5563 1305 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7420 NgoMIV G'CCGG_C Cuts at: 2759 2759 Size: 7420 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7420 NotI GC'GGCC_GC Cuts at: 4867 4867 Size: 7420 NspI r_CATG'y Cuts at: 1132 1229 3278 4317 4778 6323 1132 Size: 97 2049 1039 461 1545 2229 Fragments arranged by size: 2229 2049 1545 1039 461 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7420 PciI A'CATG_T Cuts at: 6319 6319 Size: 7420 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7420 PfoI T'CCnGG_A Cuts at: 2217 2745 2868 3030 3180 2217 Size: 528 123 162 150 6457 Fragments arranged by size: 6457 528 162 150 123 PmeI GTTT'AAAC Cuts at: 4724 4724 Size: 7420 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2882 2914 3812 3844 5489 5521 7002 7034 2882 Size: 32 898 32 1645 32 1481 32 3268 Fragments arranged by size: 3268 1645 1481 898 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2041 3034 3897 2041 Size: 993 863 5564 Fragments arranged by size: 5564 993 863 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7420 PsiI TTA'TAA Cuts at: 4990 4990 Size: 7420 PspXI vC'TCGA_Gb Cuts at: 3648 3648 Size: 7420 PstI C_TGCA'G Cuts at: 830 3625 4382 4772 830 Size: 2795 757 390 3478 Fragments arranged by size: 3478 2795 757 390 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7420 PvuII CAG'CTG Cuts at: 4553 4553 Size: 7420 SacI G_AGCT'C Cuts at: 4422 4422 Size: 7420 SacII CC_GC'GG Cuts at: 2403 2403 Size: 7420 SalI G'TCGA_C Cuts at: 1936 4762 1936 Size: 2826 4594 SanDI GG'GwC_CC Cuts at: 2041 2041 Size: 7420 SbfI CC_TGCA'GG Cuts at: 4772 4772 Size: 7420 ScaI AGT'ACT Cuts at: 5668 7380 5668 Size: 1712 5708 SfcI C'TryA_G Cuts at: 826 1045 2193 2280 3621 4378 4768 5903 6584 Size: 219 1148 87 1341 757 390 1135 681 Cuts at: 6584 6775 826 Size: 191 1471 Fragments arranged by size: 1471 1341 1148 1135 757 681 390 219 191 87 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7420 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7420 SmaI CCC'GGG Cuts at: 1872 4747 1872 Size: 2875 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2676 2778 3648 5524 6425 6687 Size: 197 926 733 102 870 1876 901 262 Cuts at: 6687 820 Size: 1553 Fragments arranged by size: 1876 1553 926 901 870 733 262 197 102 SnaBI TAC'GTA Cuts at: 493 5211 493 Size: 4718 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7420 SphI G_CATG'C Cuts at: 1132 3278 4778 1132 Size: 2146 1500 3774 Fragments arranged by size: 3774 2146 1500 SspI AAT'ATT Cuts at: 5 52 5344 5 Size: 47 5292 2081 Fragments arranged by size: 5292 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7420 StyI C'CwwG_G Cuts at: 513 1065 2370 2805 4859 513 Size: 552 1305 435 2054 3074 Fragments arranged by size: 3074 2054 1305 552 435 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2709 3377 5447 5464 5617 5802 6247 Size: 1255 1129 668 2070 17 153 185 445 Cuts at: 6247 325 Size: 1498 Fragments arranged by size: 2070 1498 1255 1129 668 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2179 2664 3048 Size: 274 80 33 51 1443 202 485 384 Cuts at: 3048 4141 4681 5666 7378 96 Size: 1093 540 985 1712 138 Fragments arranged by size: 1712 1443 1093 985 540 485 384 274 202 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7380 7412 7380 Size: 32 7388 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7420 XbaI T'CTAG_A Cuts at: 4756 4756 Size: 7420 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4538 4538 Size: 7420 XhoI C'TCGA_G Cuts at: 1943 3648 1943 Size: 1705 5715 XmaI C'CCGG_G Cuts at: 1870 4745 1870 Size: 2875 4545 XmnI GAAnn'nnTTC Cuts at: 3827 5549 3827 Size: 1722 5698 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3454 5228 276 Size: 53 83 186 936 1920 1774 2468 Fragments arranged by size: 2468 1920 1774 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI