Restriction map for
pFN21AE1731
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AvrII BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BpuAI BsaBI
BseAI BseRI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI HindIII HpaI
HphI KspI MaeIII MamI MfeI
MluI MroI MseI MslI MunI
NcoI NdeI NheI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp45I Tsp509I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 284 GGTACC G^GTACC
AccI 103 GTCTAC GT^[AC][GT]AC
AflIII 461 ACGTGT A^C[GA][CT]GT
Asp718I 284 GGTACC G^GTACC
AspI 405 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 177 CCCGAG C^[CT]CG[GA]G
AviII 711 TGCGCA TGC^GCA
BamHI 359 GGATCC G^GATCC
BfaI 413 CTAG C^TAG
BlpI 24 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 24 GCTCAGC GC^T[ACGT]AGC
BsaI 268 GGTCTC GGTCTC(1/5)
BsaWI 401 ACCGGA [AT]^CCGG[AT]
BsgI 172 CTGCAC GTGCAG(16/14)
BsoBI 177 CCCGAG C^[CT]CG[GA]G
BstYI 359 GGATCC [GA]^GATC[CT]
CelII 24 GCTCAGC GC^T[ACGT]AGC
DdeI 25 CTCAG C^T[ACGT]AG
DpnII 360 GATC ^GATC
DsaI 93 CCGTGG C^C[GA][CT]GG
EarI 271 CTCTTC CTCTTC(1/4)
EcoT14I 114 CCTTGG C^C[AT][AT]GG
FseI 481 GGCCGGCC GGCCGG^CC
FspI 711 TGCGCA TGC^GCA
HincII 190 GTCAAC GT[CT]^[GA]AC
HindII 190 GTCAAC GT[CT]^[GA]AC
HinfI 311 GACTC G^A[ACGT]TC
KpnI 284 GGTACC GGTAC^C
Ksp632I 271 CTCTTC CTCTTC(1/4)
MaeI 413 CTAG C^TAG
MboI 360 GATC ^GATC
MboII 272 TCTTC GAAGA(8/7)
MflI 359 GGATCC [GA]^GATC[CT]
NdeII 360 GATC ^GATC
NlaIII 294 CATG CATG^
NotI 182 GCGGCCGC GC^GGCCGC
PflFI 405 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 311 GACTC GAGTC(4/5)
Sau3AI 360 GATC ^GATC
SfaNI 82 GATGC GCATC(5/9)
SnaBI 381 TACGTA TAC^GTA
StyI 114 CCTTGG C^C[AT][AT]GG
TaqI 682 TCGA T^CGA
Tth111I 405 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 682 TCGA T^CGA
XhoII 359 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwI 360 GATCC GGATC(4/5)
359 GGATC
ApaI 57 GGGCCC GGGCC^C
425 GGGCCC
AspHI 109 GAGCAC G[AT]GC[AT]^C
325 GAGCAC
BalI 257 TGGCCA TGG^CCA
290 TGGCCA
BbeI 123 GGCGCC GGCGC^C
131 GGCGCC
BsaAI 381 TACGTA [CT]AC^GT[GA]
460 TACGTG
BsiEI 90 CGGCCG CG[GA][CT]^CG
183 CGGCCG
BsiHKAI 109 GAGCAC G[AT]GC[AT]^C
325 GAGCAC
BsmFI 306 GGGAC GGGAC(10/14)
557 GGGAC
BsrBI 180 GAGCGG CCGCTC(-3/-3)
355 GAGCGG
BsrFI 431 GCCGGC [GA]^CCGG[CT]
482 GCCGGC
BsrI 687 ACTGG ACTGG(1/-1)
707 CCAGT
BssSI 364 CACGAG CACGAG(-5/-1)
562 CACGAG
Cfr10I 431 GCCGGC [GA]^CCGG[CT]
482 GCCGGC
DraII 57 GGGCCCT [GA]G^G[ACGT]CC[CT]
424 GGGGCCC
EagI 90 CGGCCG C^GGCCG
183 CGGCCG
EclXI 90 CGGCCG C^GGCCG
183 CGGCCG
Eco52I 90 CGGCCG C^GGCCG
183 CGGCCG
EcoO109I 57 GGGCCCT [GA]G^G[ACGT]CC[CT]
424 GGGGCCC
HgaI 101 GCGTC GACGC(5/10)
188 GCGTC
KasI 123 GGCGCC G^GCGCC
131 GGCGCC
MluNI 257 TGGCCA TGG^CCA
290 TGGCCA
MscI 257 TGGCCA TGG^CCA
290 TGGCCA
NaeI 431 GCCGGC GCC^GGC
482 GCCGGC
NarI 123 GGCGCC GG^CGCC
131 GGCGCC
NgoMIV 431 GCCGGC G^CCGGC
482 GCCGGC
PflMI 363 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
561 CCACGAGATGG
PvuII 169 CAGCTG CAG^CTG
697 CAGCTG
SfoI 123 GGCGCC GGC^GCC
131 GGCGCC
Van91I 363 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
561 CCACGAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaII BanI BanII BbvI BcnI
BglI BmyI BpmI BsaHI BsaJI
BsiYI BslI BsmAI Bsp1286I BssKI
BstNI BstUI Cac8I CfoI Cfr13I
EaeI EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII ItaI MaeII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIV RsaI Sau96I ScrFI SfcI
TseI TspRI