(Circular) (Six-base) MAPSORT of: pFN21AE1731.seq Check: 2826 from: 1 to: 5422 pFN21AE1731 5422 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3232 278 Size: 53 83 186 936 1696 2468 Fragments arranged by size: 2468 1696 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2109 2765 1937 Size: 172 656 4594 Fragments arranged by size: 4594 656 172 Acc65I G'GTAC_C Cuts at: 2289 2743 2289 Size: 454 4968 AclI AA'CG_TT Cuts at: 1563 3549 3922 1563 Size: 1986 373 3063 Fragments arranged by size: 3063 1986 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3485 4869 5137 1632 Size: 1853 1384 268 1917 Fragments arranged by size: 1917 1853 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5225 AflIII A'CryG_T Cuts at: 1203 2466 4321 5298 1203 Size: 1263 1855 977 1327 Fragments arranged by size: 1855 1327 1263 977 AgeI A'CCGG_T Cuts at: 4253 4253 Size: 5422 AhdI GACnn_n'nnGTC Cuts at: 4151 4151 Size: 5422 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5422 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5422 AlwNI CAG_nnn'CTG Cuts at: 3165 4737 3165 Size: 1572 3850 ApaI G_GGCC'C Cuts at: 2066 2434 2066 Size: 368 5054 ApaLI G'TGCA_C Cuts at: 3479 4635 3479 Size: 1156 4266 ApoI r'AATT_y Cuts at: 1493 2731 2957 3109 5218 1493 Size: 1238 226 152 2109 1697 Fragments arranged by size: 2109 1697 1238 226 152 AseI AT'TA_AT Cuts at: 160 1032 3976 160 Size: 872 2944 1606 Fragments arranged by size: 2944 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5422 AvaI C'yCGr_G Cuts at: 1870 1943 2182 2747 1870 Size: 73 239 565 4545 Fragments arranged by size: 4545 565 239 73 BamHI G'GATC_C Cuts at: 2364 2752 3119 2364 Size: 388 367 4667 Fragments arranged by size: 4667 388 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2128 2136 2289 2743 4198 Size: 325 217 627 341 8 153 454 1455 Cuts at: 4198 618 Size: 1842 Fragments arranged by size: 1842 1455 627 454 341 325 217 153 8 BanII G_rGCy'C Cuts at: 1391 2046 2066 2074 2434 2654 2670 2699 2741 Size: 655 20 8 360 220 16 29 42 Cuts at: 2741 1391 Size: 4072 Fragments arranged by size: 4072 655 360 220 42 29 20 16 8 BbeI G_GCGC'C Cuts at: 2132 2140 2132 Size: 8 5414 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4451 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3613 3647 1903 Size: 34 1676 34 3678 Fragments arranged by size: 3678 1676 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3314 4524 3314 Size: 1210 4212 BclI T'GATC_A Cuts at: 1548 1548 Size: 5422 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2050 2103 2632 Size: 107 122 71 631 605 378 53 529 Cuts at: 2632 4033 136 Size: 1401 1525 Fragments arranged by size: 1525 1401 631 605 529 378 122 107 71 53 BglII A'GATC_T Cuts at: 5417 5417 Size: 5422 BlpI GC'TnA_GC Cuts at: 2030 2839 2030 Size: 809 4613 Bme1580I G_kGCm'C Cuts at: 1790 2066 2434 3483 4639 1790 Size: 276 368 1049 1156 2573 Fragments arranged by size: 2573 1156 1049 368 276 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5422 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2702 3153 4111 Size: 416 53 83 399 24 1285 451 958 Cuts at: 4111 442 Size: 1753 Fragments arranged by size: 1753 1285 958 451 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5422 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 2156 2228 2684 2726 4082 1239 Size: 821 96 72 456 42 1356 2579 Fragments arranged by size: 2579 1356 821 456 96 72 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3547 4412 4710 3547 Size: 865 298 4259 Fragments arranged by size: 4259 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2279 4085 882 Size: 617 780 1806 2219 Fragments arranged by size: 2219 1806 780 617 BsaAI yAC'GTr Cuts at: 493 1204 2388 2467 3213 493 Size: 711 1184 79 746 2702 Fragments arranged by size: 2702 1184 746 711 79 BsaBI GATnn'nnATC Cuts at: 5416 5416 Size: 5422 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2106 2129 2137 3229 Size: 53 83 186 936 573 23 8 1092 Cuts at: 3229 3611 4246 275 Size: 382 635 1451 Fragments arranged by size: 1451 1092 936 635 573 382 186 83 53 23 8 BsaWI w'CCGG_w Cuts at: 2406 3855 4253 4527 4674 5085 2406 Size: 1449 398 274 147 411 2743 Fragments arranged by size: 2743 1449 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5197 Fragments arranged by size: 5197 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5422 BseYI C'CCAG_C Cuts at: 2374 2444 2700 4625 5121 2374 Size: 70 256 1925 496 2675 Fragments arranged by size: 2675 1925 496 256 70 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2162 1163 Size: 573 426 4423 Fragments arranged by size: 4423 573 426 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2098 2191 2872 3633 4661 1540 Size: 275 186 97 93 681 761 1028 2301 Fragments arranged by size: 2301 1028 761 681 275 186 97 93 BsiHKAI G_wGCw'C Cuts at: 2118 2334 3483 3568 4639 2118 Size: 216 1149 85 1071 2901 Fragments arranged by size: 2901 1149 1071 216 85 BsmI GAATG_Cn' Cuts at: 2933 3026 2933 Size: 93 5329 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2066 2074 2118 2334 2434 2654 Size: 399 256 20 8 44 216 100 220 Cuts at: 2654 2670 2699 2741 3483 3568 4639 1391 Size: 16 29 42 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 256 220 216 100 85 44 42 29 20 16 8 BspEI T'CCGG_A Cuts at: 5085 5085 Size: 5422 BspHI T'CATG_A Cuts at: 3311 5041 3311 Size: 1730 3692 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2777 844 Size: 1933 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2187 2362 3309 1394 Size: 429 364 175 947 3507 Fragments arranged by size: 3507 947 429 364 175 BsrDI GCAATG_nn' Cuts at: 65 1248 3917 4091 65 Size: 1183 2669 174 1396 Fragments arranged by size: 2669 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2436 2487 4066 4253 1700 Size: 110 626 51 1579 187 2869 Fragments arranged by size: 2869 1579 626 187 110 51 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5422 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5422 BssSI C'ACGA_G Cuts at: 2369 2567 3482 4494 2369 Size: 198 915 1012 3297 Fragments arranged by size: 3297 1012 915 198 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5422 BstBI TT'CG_AA Cuts at: 4226 4226 Size: 5422 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5422 BstYI r'GATC_y Cuts at: 1972 2364 2752 3119 3504 3521 4973 5059 5071 Size: 392 388 367 385 17 1452 86 12 Cuts at: 5071 5417 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 392 388 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2098 513 Size: 552 939 94 3837 Fragments arranged by size: 3837 939 552 94 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2100 487 Size: 683 56 874 3809 Fragments arranged by size: 3809 874 683 56 BtsI GCAGTG_nn' Cuts at: 2250 2938 3732 3752 2250 Size: 688 794 20 3920 Fragments arranged by size: 3920 794 688 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5422 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5387 DraI TTT'AAA Cuts at: 2726 2726 Size: 5422 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4429 809 Size: 507 3113 1802 Fragments arranged by size: 3113 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2095 2188 2262 Size: 54 1425 50 275 18 265 93 74 Cuts at: 2262 2295 2869 3758 5387 8 Size: 33 574 889 1629 43 Fragments arranged by size: 1629 1425 889 574 275 265 93 74 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2095 2188 2869 1537 Size: 275 283 93 681 4090 Fragments arranged by size: 4090 681 283 275 93 EarI CTCTTCn'nnn_ Cuts at: 1353 2282 3352 1353 Size: 929 1070 3423 Fragments arranged by size: 3423 1070 929 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4009 4383 4529 387 Size: 1064 138 2420 374 146 1280 Fragments arranged by size: 2420 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2156 2228 2684 2726 3485 4082 Size: 393 428 96 72 456 42 759 597 Cuts at: 4082 4869 5137 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 759 597 456 428 393 268 96 72 42 EcoO109I rG'GnC_Cy Cuts at: 2063 2430 2063 Size: 367 5055 EcoRI G'AATT_C Cuts at: 2731 3109 2731 Size: 378 5044 FseI GG_CCGG'CC Cuts at: 2491 2491 Size: 5422 FspI TGC'GCA Cuts at: 2718 3928 2718 Size: 1210 4212 FspAI rTGC'GCAy Cuts at: 2718 2718 Size: 5422 HaeII r_GCGC'y Cuts at: 1678 2132 2140 2220 2343 2451 4569 1678 Size: 454 8 80 123 108 2118 2531 Fragments arranged by size: 2531 2118 454 123 108 80 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4063 4095 4137 4169 1373 Size: 32 135 32 2491 32 42 32 2626 Fragments arranged by size: 2626 2491 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2197 2766 3012 669 Size: 988 281 259 569 246 3079 Fragments arranged by size: 3079 988 569 281 259 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5422 HpaI GTT'AAC Cuts at: 3012 3012 Size: 5422 KasI G'GCGC_C Cuts at: 2128 2136 2128 Size: 8 5414 KpnI G_GTAC'C Cuts at: 2293 2747 2293 Size: 454 4968 MfeI C'AATT_G Cuts at: 3021 3021 Size: 5422 MluI A'CGCG_T Cuts at: 5298 5298 Size: 5422 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4536 4720 1305 Size: 3231 184 2007 Fragments arranged by size: 3231 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2264 2297 10 Size: 54 1425 343 432 33 3135 Fragments arranged by size: 3135 1425 432 343 54 33 MslI CAynn'nnrTG Cuts at: 518 1166 3380 3739 3898 518 Size: 648 2214 359 159 2042 Fragments arranged by size: 2214 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2060 2176 2704 2838 3515 4663 4908 1606 Size: 454 116 528 134 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 528 454 245 134 116 NaeI GCC'GGC Cuts at: 2438 2489 2438 Size: 51 5371 NarI GG'CG_CC Cuts at: 2129 2137 2129 Size: 8 5414 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3931 Fragments arranged by size: 3931 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5422 NgoMIV G'CCGG_C Cuts at: 2436 2487 2436 Size: 51 5371 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5422 NotI GC'GGCC_GC Cuts at: 2188 2869 2188 Size: 681 4741 NspI r_CATG'y Cuts at: 1132 1229 2780 4325 1132 Size: 97 1551 1545 2229 Fragments arranged by size: 2229 1551 1545 97 PasI CC'CwG_GG Cuts at: 1348 2066 1348 Size: 718 4704 PciI A'CATG_T Cuts at: 4321 4321 Size: 5422 PflMI CCAn_nnn'nTGG Cuts at: 1108 2374 2572 1108 Size: 1266 198 3958 Fragments arranged by size: 3958 1266 198 PmeI GTTT'AAAC Cuts at: 2726 2726 Size: 5422 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3491 3523 5004 5036 3491 Size: 32 1481 32 3877 Fragments arranged by size: 3877 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5422 PsiI TTA'TAA Cuts at: 2992 2992 Size: 5422 PspOMI G'GGCC_C Cuts at: 2062 2430 2062 Size: 368 5054 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2204 2236 2204 Size: 32 5390 PstI C_TGCA'G Cuts at: 830 2774 830 Size: 1944 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5422 PvuII CAG'CTG Cuts at: 2176 2704 2176 Size: 528 4894 SalI G'TCGA_C Cuts at: 1936 2764 1936 Size: 828 4594 SbfI CC_TGCA'GG Cuts at: 2774 2774 Size: 5422 ScaI AGT'ACT Cuts at: 3670 5382 3670 Size: 1712 3710 SfcI C'TryA_G Cuts at: 826 1045 2221 2335 2585 2770 3905 4586 4777 Size: 219 1176 114 250 185 1135 681 191 Cuts at: 4777 826 Size: 1471 Fragments arranged by size: 1471 1176 1135 681 250 219 191 185 114 SfoI GGC'GCC Cuts at: 2130 2138 2130 Size: 8 5414 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5422 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5422 SmaI CCC'GGG Cuts at: 1872 2749 1872 Size: 877 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3526 4427 4689 820 Size: 197 926 1583 901 262 1553 Fragments arranged by size: 1583 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2388 3213 493 Size: 1895 825 2702 Fragments arranged by size: 2702 1895 825 SpeI A'CTAG_T Cuts at: 152 152 Size: 5422 SphI G_CATG'C Cuts at: 1132 2780 1132 Size: 1648 3774 SspI AAT'ATT Cuts at: 5 52 3346 5 Size: 47 3294 2081 Fragments arranged by size: 3294 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5422 StyI C'CwwG_G Cuts at: 513 1065 2004 2119 2861 513 Size: 552 939 115 742 3074 Fragments arranged by size: 3074 939 742 552 115 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3449 3466 3619 3804 4249 325 Size: 1255 1869 17 153 185 445 1498 Fragments arranged by size: 1869 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3668 5380 96 Size: 274 80 33 51 1443 1691 1712 138 Fragments arranged by size: 1712 1691 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5382 5414 5382 Size: 32 5390 Tth111I GACn'n_nGTC Cuts at: 1316 2413 1316 Size: 1097 4325 XbaI T'CTAG_A Cuts at: 2758 2758 Size: 5422 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5422 XmaI C'CCGG_G Cuts at: 1870 2747 1870 Size: 877 4545 XmnI GAAnn'nnTTC Cuts at: 3551 3551 Size: 5422 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3230 276 Size: 53 83 186 936 1696 2468 Fragments arranged by size: 2468 1696 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI NruI NsiI PacI PfoI PmlI PpuMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI