Restriction map for pFN21AE1731
 (Linear) (Six-base) MAPSORT of: KIEE1731.seq  Check: 9373  from: 1  to: 717



KIEE1731.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AccI GT'mk_AC

Cuts at:      0     104     717 
   Size:        104     613
 
Acc65I G'GTAC_C

Cuts at:      0     284     717 
   Size:        284     433
 
AflIII A'CryG_T

Cuts at:      0     461     717 
   Size:        461     256
 
ApaI G_GGCC'C

Cuts at:      0      61     429     717 
   Size:         61     368     288

  Fragments arranged by size: 

                 368     288      61
 
AvaI C'yCGr_G

Cuts at:      0     177     717 
   Size:        177     540
 
BamHI G'GATC_C

Cuts at:      0     359     717 
   Size:        359     358
 
BanI G'GyrC_C

Cuts at:      0     123     131     284     717 
   Size:        123       8     153     433

  Fragments arranged by size: 

                 433     153     123       8
 
BanII G_rGCy'C

Cuts at:      0      41      61      69     429     649     665     694     717 
   Size:         41      20       8     360     220      16      29      23

  Fragments arranged by size: 

                 360     220      41      29      23      20      16       8
            
 
BbeI G_GCGC'C

Cuts at:      0     127     135     717 
   Size:        127       8     582

  Fragments arranged by size: 

                 582     127       8
 
BglI GCCn_nnn'nGGC

Cuts at:      0      45      98     627     717 
   Size:         45      53     529      90

  Fragments arranged by size: 

                 529      90      53      45
 
BlpI GC'TnA_GC

Cuts at:      0      25     717 
   Size:         25     692
 
Bme1580I G_kGCm'C

Cuts at:      0      61     429     717 
   Size:         61     368     288

  Fragments arranged by size: 

                 368     288      61
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     697     717 
   Size:        697      20
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     151     223     679     717 
   Size:         55      96      72     456      38

  Fragments arranged by size: 

                 456      96      72      55      38
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     274     717 
   Size:        274     443
 
BsaAI yAC'GTr

Cuts at:      0     383     462     717 
   Size:        383      79     255

  Fragments arranged by size: 

                 383     255      79
 
BsaHI Gr'CG_yC

Cuts at:      0     101     124     132     717 
   Size:        101      23       8     585

  Fragments arranged by size: 

                 585     101      23       8
 
BsaWI w'CCGG_w

Cuts at:      0     401     717 
   Size:        401     316
 
BseYI C'CCAG_C

Cuts at:      0     369     439     695     717 
   Size:        369      70     256      22

  Fragments arranged by size: 

                 369     256      70      22
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     717 
   Size:        157     560
 
BsiEI CG_ry'CG

Cuts at:      0      93     186     717 
   Size:         93      93     531

  Fragments arranged by size: 

                 531      93      93
 
BsiHKAI G_wGCw'C

Cuts at:      0     113     329     717 
   Size:        113     216     388

  Fragments arranged by size: 

                 388     216     113
 
Bsp1286I G_dGCh'C

Cuts at:      0      41      61      69     113     329     429     649     665 
   Size:         41      20       8      44     216     100     220      16

Cuts at:    665     694     717 
   Size:         29      23

  Fragments arranged by size: 

                 220     216     100      44      41      29      23      20
                  16       8
 
BsrBI CCG'CTC

Cuts at:      0     182     357     717 
   Size:        182     175     360

  Fragments arranged by size: 

                 360     182     175
 
BsrFI r'CCGG_y

Cuts at:      0     431     482     717 
   Size:        431      51     235

  Fragments arranged by size: 

                 431     235      51
 
BssSI C'ACGA_G

Cuts at:      0     364     562     717 
   Size:        364     198     155

  Fragments arranged by size: 

                 364     198     155
 
BstYI r'GATC_y

Cuts at:      0     359     717 
   Size:        359     358
 
BtgI C'CryG_G

Cuts at:      0      93     717 
   Size:         93     624
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      95     717 
   Size:         95     622
 
BtsI GCAGTG_nn'

Cuts at:      0     245     717 
   Size:        245     472
 
EaeI y'GGCC_r

Cuts at:      0      90     183     257     290     717 
   Size:         90      93      74      33     427

  Fragments arranged by size: 

                 427      93      90      74      33
 
EagI C'GGCC_G

Cuts at:      0      90     183     717 
   Size:         90      93     534

  Fragments arranged by size: 

                 534      93      90
 
EarI CTCTTCn'nnn_

Cuts at:      0     277     717 
   Size:        277     440
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     151     223     679     717 
   Size:         55      96      72     456      38

  Fragments arranged by size: 

                 456      96      72      55      38
 
EcoO109I rG'GnC_Cy

Cuts at:      0      58     425     717 
   Size:         58     367     292

  Fragments arranged by size: 

                 367     292      58
 
FseI GG_CCGG'CC

Cuts at:      0     486     717 
   Size:        486     231
 
FspI TGC'GCA

Cuts at:      0     713     717 
   Size:        713       4
 
FspAI rTGC'GCAy

Cuts at:      0     713     717 
   Size:        713       4
 
HaeII r_GCGC'y

Cuts at:      0     127     135     215     338     446     717 
   Size:        127       8      80     123     108     271

  Fragments arranged by size: 

                 271     127     123     108      80       8
 
HincII GTy'rAC

Cuts at:      0     192     717 
   Size:        192     525
 
KasI G'GCGC_C

Cuts at:      0     123     131     717 
   Size:        123       8     586

  Fragments arranged by size: 

                 586     123       8
 
KpnI G_GTAC'C

Cuts at:      0     288     717 
   Size:        288     429
 
MscI TGG'CCA

Cuts at:      0     259     292     717 
   Size:        259      33     425

  Fragments arranged by size: 

                 425     259      33
 
MspA1I CmG'CkG

Cuts at:      0      55     171     699     717 
   Size:         55     116     528      18

  Fragments arranged by size: 

                 528     116      55      18
 
NaeI GCC'GGC

Cuts at:      0     433     484     717 
   Size:        433      51     233

  Fragments arranged by size: 

                 433     233      51
 
NarI GG'CG_CC

Cuts at:      0     124     132     717 
   Size:        124       8     585

  Fragments arranged by size: 

                 585     124       8
 
NgoMIV G'CCGG_C

Cuts at:      0     431     482     717 
   Size:        431      51     235

  Fragments arranged by size: 

                 431     235      51
 
NotI GC'GGCC_GC

Cuts at:      0     183     717 
   Size:        183     534
 
PasI CC'CwG_GG

Cuts at:      0      61     717 
   Size:         61     656
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     369     567     717 
   Size:        369     198     150

  Fragments arranged by size: 

                 369     198     150
 
PspOMI G'GGCC_C

Cuts at:      0      57     425     717 
   Size:         57     368     292

  Fragments arranged by size: 

                 368     292      57
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     199     231     717 
   Size:        199      32     486

  Fragments arranged by size: 

                 486     199      32
 
PvuII CAG'CTG

Cuts at:      0     171     699     717 
   Size:        171     528      18

  Fragments arranged by size: 

                 528     171      18
 
SfcI C'TryA_G

Cuts at:      0     216     330     580     717 
   Size:        216     114     250     137

  Fragments arranged by size: 

                 250     216     137     114
 
SfoI GGC'GCC

Cuts at:      0     125     133     717 
   Size:        125       8     584

  Fragments arranged by size: 

                 584     125       8
 
SnaBI TAC'GTA

Cuts at:      0     383     717 
   Size:        383     334
 
StyI C'CwwG_G

Cuts at:      0     114     717 
   Size:        114     603
 
Tth111I GACn'n_nGTC

Cuts at:      0     408     717 
   Size:        408     309
 

 Enzymes that do cut:

     AccI   Acc65I   AflIII     ApaI     AvaI    BamHI     BanI    BanII
     BbeI     BglI     BlpI Bme1580I     BmrI     BpmI     BsaI    BsaAI
    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BsrBI
    BsrFI    BssSI    BstYI     BtgI    BtgZI     BtsI     EaeI     EagI
     EarI  Eco57MI EcoO109I     FseI     FspI    FspAI    HaeII   HincII
     KasI     KpnI     MscI   MspA1I     NaeI     NarI   NgoMIV     NotI
     PasI    PflMI   PspOMI     PsrI    PvuII     SfcI     SfoI    SnaBI
     StyI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII    BmgBI     BmtI     BplI   Bpu10I    BpuEI    BsaBI
    BsaXI    BseRI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrDI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI    Hin4I  HindIII     HpaI     MfeI
     MluI     MmeI     MslI     NcoI     NdeI     NheI     NruI     NsiI
     NspI     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI     SmlI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI
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