(Linear) (Six-base) MAPSORT of: KIBB5788.seq Check: 614 from: 1 to: 1056 KIBB5788.orf With 182 enzymes: SgfI * July 3, 2012 16:06 .. AccI GT'mk_AC Cuts at: 0 659 992 1056 Size: 659 333 64 Fragments arranged by size: 659 333 64 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 155 604 643 1056 Size: 155 449 39 413 Fragments arranged by size: 449 413 155 39 AflIII A'CryG_T Cuts at: 0 938 1056 Size: 938 118 AhdI GACnn_n'nnGTC Cuts at: 0 1026 1056 Size: 1026 30 AvaI C'yCGr_G Cuts at: 0 485 1056 Size: 485 571 BanII G_rGCy'C Cuts at: 0 11 494 1056 Size: 11 483 562 Fragments arranged by size: 562 483 11 BbsI GAAGACnn'nnnn_ Cuts at: 0 176 527 1056 Size: 176 351 529 Fragments arranged by size: 529 351 176 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1012 1046 1056 Size: 1012 34 10 Fragments arranged by size: 1012 34 10 BglI GCCn_nnn'nGGC Cuts at: 0 256 314 1039 1056 Size: 256 58 725 17 Fragments arranged by size: 725 256 58 17 BlpI GC'TnA_GC Cuts at: 0 5 1056 Size: 5 1051 Bme1580I G_kGCm'C Cuts at: 0 572 1056 Size: 572 484 BmgBI CAC'GTC Cuts at: 0 819 941 1056 Size: 819 122 115 Fragments arranged by size: 819 122 115 BmtI G_CTAG'C Cuts at: 0 1018 1056 Size: 1018 38 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 287 1056 Size: 287 769 Bpu10I CC'TnA_GC Cuts at: 0 676 1056 Size: 676 380 BsaAI yAC'GTr Cuts at: 0 189 1056 Size: 189 867 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 895 925 1056 Size: 895 30 131 Fragments arranged by size: 895 131 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 957 1056 Size: 957 99 BseYI C'CCAG_C Cuts at: 0 43 160 373 1001 1056 Size: 43 117 213 628 55 Fragments arranged by size: 628 213 117 55 43 BsiWI C'GTAC_G Cuts at: 0 50 1056 Size: 50 1006 BsmBI CGTCTCn'nnnn_ Cuts at: 0 947 1056 Size: 947 109 Bsp1286I G_dGCh'C Cuts at: 0 11 494 572 1056 Size: 11 483 78 484 Fragments arranged by size: 484 483 78 11 BsrBI CCG'CTC Cuts at: 0 358 452 1056 Size: 358 94 604 Fragments arranged by size: 604 358 94 BsrDI GCAATG_nn' Cuts at: 0 966 1056 Size: 966 90 BsrFI r'CCGG_y Cuts at: 0 973 1056 Size: 973 83 BsrGI T'GTAC_A Cuts at: 0 911 1056 Size: 911 145 BstBI TT'CG_AA Cuts at: 0 23 1056 Size: 23 1033 BstEII G'GTnAC_C Cuts at: 0 684 1056 Size: 684 372 BstXI CCAn_nnnn'nTGG Cuts at: 0 501 823 1056 Size: 501 322 233 Fragments arranged by size: 501 322 233 BtgI C'CryG_G Cuts at: 0 286 894 946 1056 Size: 286 608 52 110 Fragments arranged by size: 608 286 110 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 251 1056 Size: 251 805 BtsI GCAGTG_nn' Cuts at: 0 512 1056 Size: 512 544 EaeI y'GGCC_r Cuts at: 0 735 1040 1056 Size: 735 305 16 Fragments arranged by size: 735 305 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 528 842 916 1056 Size: 528 314 74 140 Fragments arranged by size: 528 314 140 74 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 155 287 604 643 1056 Size: 155 132 317 39 413 Fragments arranged by size: 413 317 155 132 39 EcoNI CCTnn'n_nnAGG Cuts at: 0 679 827 1056 Size: 679 148 229 Fragments arranged by size: 679 229 148 EcoO109I rG'GnC_Cy Cuts at: 0 797 1056 Size: 797 259 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 783 879 1056 Size: 783 96 177 Fragments arranged by size: 783 177 96 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 21 53 183 215 1012 1044 1056 Size: 21 32 130 32 797 32 12 Fragments arranged by size: 797 130 32 32 32 21 12 HincII GTy'rAC Cuts at: 0 672 1056 Size: 672 384 HindIII A'AGCT_T Cuts at: 0 760 1056 Size: 760 296 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 496 1056 Size: 496 560 MscI TGG'CCA Cuts at: 0 737 1042 1056 Size: 737 305 14 Fragments arranged by size: 737 305 14 MslI CAynn'nnrTG Cuts at: 0 985 1056 Size: 985 71 MspA1I CmG'CkG Cuts at: 0 288 410 1056 Size: 288 122 646 Fragments arranged by size: 646 288 122 NheI G'CTAG_C Cuts at: 0 1014 1056 Size: 1014 42 PflMI CCAn_nnn'nTGG Cuts at: 0 733 1056 Size: 733 323 PmlI CAC'GTG Cuts at: 0 189 1056 Size: 189 867 PpuMI rG'GwC_Cy Cuts at: 0 797 1056 Size: 797 259 PstI C_TGCA'G Cuts at: 0 557 1056 Size: 557 499 SacII CC_GC'GG Cuts at: 0 289 1056 Size: 289 767 SanDI GG'GwC_CC Cuts at: 0 797 1056 Size: 797 259 SfcI C'TryA_G Cuts at: 0 414 553 1056 Size: 414 139 503 Fragments arranged by size: 503 414 139 SfiI GGCCn_nnn'nGGCC Cuts at: 0 314 1056 Size: 314 742 SmaI CCC'GGG Cuts at: 0 487 1056 Size: 487 569 StyI C'CwwG_G Cuts at: 0 251 1056 Size: 251 805 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 387 495 537 829 1056 Size: 387 108 42 292 227 Fragments arranged by size: 387 292 227 108 42 TatI w'GTAC_w Cuts at: 0 911 1056 Size: 911 145 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 321 353 1056 Size: 321 32 703 Fragments arranged by size: 703 321 32 Tth111I GACn'n_nGTC Cuts at: 0 778 1056 Size: 778 278 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 790 1056 Size: 790 266 XmaI C'CCGG_G Cuts at: 0 485 1056 Size: 485 571 Enzymes that do cut: AccI AcuI AflIII AhdI AvaI BanII BbsI BcgI BglI BlpI Bme1580I BmgBI BmtI BpmI Bpu10I BsaAI BsaXI BseRI BseYI BsiWI BsmBI Bsp1286I BsrBI BsrDI BsrFI BsrGI BstBI BstEII BstXI BtgI BtgZI BtsI EaeI EciI Eco57MI EcoNI EcoO109I FalI Hin4I HincII HindIII MmeI MscI MslI MspA1I NheI PflMI PmlI PpuMI PstI SacII SanDI SfcI SfiI SmaI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BciVI BclI BfrBI BglII BmrI BplI BpuEI BsaI BsaBI BsaHI BsaWI BsgI BsiEI BsiHKAI BsmI BspEI BspHI BspMI BssHII BssSI BstAPI BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XhoI XmnI ZraI