################################## # NO CUT ENZYMES ( 184 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BsrI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MboII MfeI MluI MluNI MroI MscI MseI MslI MunI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I TspRI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccII 133 CGCG CG^CG ApaI 50 GGGCCC GGGCC^C AscI 131 GGCGCGCC GG^CGCGCC AspHI 124 GTGCTC G[AT]GC[AT]^C BamHI 149 GGATCC G^GATCC BanII 50 GGGCCC G[GA]GC[CT]^C BsaWI 146 ACCGGA [AT]^CCGG[AT] BsgI 247 GTGCAG GTGCAG(16/14) BsiHKAI 124 GTGCTC G[AT]GC[AT]^C BsrBI 162 GAGCGG CCGCTC(-3/-3) BsrFI 31 GCCGGC [GA]^CCGG[CT] BssHII 132 GCGCGC G^CGCGC BstUI 133 CGCG CG^CG BstYI 149 GGATCC [GA]^GATC[CT] Cfr10I 31 GCCGGC [GA]^CCGG[CT] DpnII 150 GATC ^GATC EaeI 29 TGGCCG [CT]^GGCC[GA] EcoRV 426 GATATC GAT^ATC HphI 159 GGTGA GGTGA(8/7) MboI 150 GATC ^GATC MflI 149 GGATCC [GA]^GATC[CT] MspA1I 56 CCGCTG C[AC]G^C[GT]G MvnI 133 CGCG CG^CG MwoI 164 GCGGGAGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NaeI 31 GCCGGC GCC^GGC NdeII 150 GATC ^GATC NgoMIV 31 GCCGGC G^CCGGC PflMI 110 CCATTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Sau3AI 150 GATC ^GATC SfaNI 271 GATGC GCATC(5/9) TaqI 276 TCGA T^CGA Tsp509I 436 AATT ^AATT TthHB8I 276 TCGA T^CGA Van91I 110 CCATTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 149 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AciI 56 CCGC CCGC(-3/-1) 164 GCGG AlwI 150 GATCC GGATC(4/5) 149 GGATC BmyI 50 GGGCCC G[AGT]GC[ACT]^C 124 GTGCTC BsmAI 207 GAGAC GTCTC(1/5) 415 GTCTC Bsp1286I 50 GGGCCC G[AGT]GC[ACT]^C 124 GTGCTC BstF5I 374 CATCC GGATG(2/0) 270 GGATG CfoI 132 GCGC GCG^C 134 GCGC DraII 50 GGGCCCC [GA]G^G[ACGT]CC[CT] 324 AGGCCCT EcoO109I 50 GGGCCCC [GA]G^G[ACGT]CC[CT] 324 AGGCCCT EcoT14I 69 CCAAGG C^C[AT][AT]GG 321 CCAAGG FauI 55 CCCGC CCCGC(4/6) 164 GCGGG FokI 374 CATCC GGATG(9/13) 270 GGATG HhaI 132 GCGC GCG^C 134 GCGC HinP1I 132 GCGC G^CGC 134 GCGC NlaIII 258 CATG CATG^ 331 CATG StyI 69 CCAAGG C^C[AT][AT]GG 321 CCAAGG XcmI 102 CCACTACCCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 257 CCATGAAGCTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AluI BbvI BsaJI BsiYI BslI BssKI BstNI Cac8I Cfr13I EcoRII Fnu4HI HaeIII HapII HpaII ItaI MnlI MspI MvaI NlaIV Sau96I ScrFI TseI