(Circular) (Six-base) MAPSORT of: pFN21AB7329.seq Check: 2665 from: 1 to: 7405 pFN21AB7329 7405 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3498 3498 Size: 7405 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3365 5215 278 Size: 53 83 186 936 1829 1850 2468 Fragments arranged by size: 2468 1850 1829 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2684 3542 4748 1937 Size: 747 858 1206 4594 Fragments arranged by size: 4594 1206 858 747 Acc65I G'GTAC_C Cuts at: 4522 4726 4522 Size: 204 7201 AclI AA'CG_TT Cuts at: 1563 5532 5905 1563 Size: 3969 373 3063 Fragments arranged by size: 3969 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2263 2367 3227 3269 3950 4502 5468 6852 Size: 631 104 860 42 681 552 966 1384 Cuts at: 6852 7120 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 966 860 681 631 552 268 104 42 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7208 AflIII A'CryG_T Cuts at: 1203 6304 7281 1203 Size: 5101 977 1327 Fragments arranged by size: 5101 1327 977 AgeI A'CCGG_T Cuts at: 6236 6236 Size: 7405 AhdI GACnn_n'nnGTC Cuts at: 6134 6134 Size: 7405 AleI CACnn'nnGTG Cuts at: 1166 4361 1166 Size: 3195 4210 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7405 AlwNI CAG_nnn'CTG Cuts at: 4367 5148 6720 4367 Size: 781 1572 5052 Fragments arranged by size: 5052 1572 781 ApaI G_GGCC'C Cuts at: 4595 4595 Size: 7405 ApaLI G'TGCA_C Cuts at: 5462 6618 5462 Size: 1156 6249 ApoI r'AATT_y Cuts at: 1493 2560 3163 3784 4714 4940 5092 7201 1493 Size: 1067 603 621 930 226 152 2109 1697 Fragments arranged by size: 2109 1697 1067 930 621 603 226 152 AseI AT'TA_AT Cuts at: 160 1032 2460 5959 160 Size: 872 1428 3499 1606 Fragments arranged by size: 3499 1606 1428 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7405 AvaI C'yCGr_G Cuts at: 1870 1943 3823 4170 4730 1870 Size: 73 1880 347 560 4545 Fragments arranged by size: 4545 1880 560 347 73 BamHI G'GATC_C Cuts at: 4735 5102 4735 Size: 367 7038 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4522 4570 4726 6181 618 Size: 325 217 627 2735 48 156 1455 1842 Fragments arranged by size: 2735 1842 1455 627 325 217 156 48 BanII G_rGCy'C Cuts at: 1391 3382 3674 4595 4724 1391 Size: 1991 292 921 129 4072 Fragments arranged by size: 4072 1991 921 292 129 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2193 2201 3215 3365 3721 3807 4345 Size: 971 294 8 1014 150 356 86 538 Cuts at: 4345 928 Size: 3988 Fragments arranged by size: 3988 1014 971 538 356 294 150 86 8 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3257 3291 5596 5630 1903 Size: 34 1320 34 2305 34 3678 Fragments arranged by size: 3678 2305 1320 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5297 6507 5297 Size: 1210 6195 BclI T'GATC_A Cuts at: 1548 1548 Size: 7405 BfrBI ATG'CAT Cuts at: 2089 2844 2089 Size: 755 6650 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6016 136 Size: 107 122 71 631 605 4344 1525 Fragments arranged by size: 4344 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7400 7400 Size: 7405 BlpI GC'TnA_GC Cuts at: 4822 4822 Size: 7405 Bme1580I G_kGCm'C Cuts at: 1790 3999 4276 4595 5466 6622 1790 Size: 2209 277 319 871 1156 2573 Fragments arranged by size: 2573 2209 1156 871 319 277 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7405 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4114 4180 5136 Size: 416 53 83 399 24 2697 66 956 Cuts at: 5136 6094 442 Size: 958 1753 Fragments arranged by size: 2697 1753 958 956 416 399 83 66 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7405 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3482 3482 Size: 7405 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2041 2837 3933 6065 1239 Size: 802 796 1096 2132 2579 Fragments arranged by size: 2579 2132 1096 802 796 Bpu10I CC'TnA_GC Cuts at: 2967 2967 Size: 7405 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3154 3371 4514 5530 6395 6693 3154 Size: 217 1143 1016 865 298 3866 Fragments arranged by size: 3866 1143 1016 865 298 217 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3567 4225 6068 882 Size: 617 2068 658 1843 2219 Fragments arranged by size: 2219 2068 1843 658 617 BsaAI yAC'GTr Cuts at: 493 1204 3197 5196 493 Size: 711 1993 1999 2702 Fragments arranged by size: 2702 1999 1993 711 BsaBI GATnn'nnATC Cuts at: 7399 7399 Size: 7405 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2544 3362 5212 5594 Size: 53 83 186 936 1011 818 1850 382 Cuts at: 5594 6229 275 Size: 635 1451 Fragments arranged by size: 1850 1451 1011 936 818 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3919 5838 6236 6510 6657 7068 3919 Size: 1919 398 274 147 411 4256 Fragments arranged by size: 4256 1919 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3347 3377 4606 4636 1178 Size: 30 165 30 1944 30 1229 30 3947 Fragments arranged by size: 3947 1944 1229 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2259 3362 1186 Size: 1073 1103 5229 Fragments arranged by size: 5229 1103 1073 BseYI C'CCAG_C Cuts at: 2700 4625 4665 6608 7104 2700 Size: 1925 40 1943 496 3001 Fragments arranged by size: 3001 1943 1925 496 40 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3470 4476 4517 1163 Size: 573 1734 1006 41 4051 Fragments arranged by size: 4051 1734 1006 573 41 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4552 4855 5616 6644 1540 Size: 275 186 2551 303 761 1028 2301 Fragments arranged by size: 2551 2301 1028 761 303 275 186 BsiHKAI G_wGCw'C Cuts at: 2865 5466 5551 6622 2865 Size: 2601 85 1071 3648 Fragments arranged by size: 3648 2601 1071 85 BsmI GAATG_Cn' Cuts at: 2089 3894 4916 5009 2089 Size: 1805 1022 93 4485 Fragments arranged by size: 4485 1805 1022 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3389 3389 Size: 7405 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2865 3382 3674 3999 4276 4595 4724 Size: 399 1075 517 292 325 277 319 129 Cuts at: 4724 5466 5551 6622 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1075 1071 742 517 399 325 319 292 277 129 85 BspEI T'CCGG_A Cuts at: 3919 7068 3919 Size: 3149 4256 BspHI T'CATG_A Cuts at: 4255 5294 7024 4255 Size: 1039 1730 4636 Fragments arranged by size: 4636 1730 1039 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3498 3604 4760 844 Size: 2654 106 1156 3489 Fragments arranged by size: 3489 2654 1156 106 BsrBI CCG'CTC Cuts at: 1394 1823 2053 4078 4554 5292 1394 Size: 429 230 2025 476 738 3507 Fragments arranged by size: 3507 2025 738 476 429 230 BsrDI GCAATG_nn' Cuts at: 65 1248 3010 5900 6074 65 Size: 1183 1762 2890 174 1396 Fragments arranged by size: 2890 1762 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4547 6049 6236 1700 Size: 110 2737 1502 187 2869 Fragments arranged by size: 2869 2737 1502 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7405 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7405 BssSI C'ACGA_G Cuts at: 5465 6477 5465 Size: 1012 6393 BstAPI GCAn_nnn'nTGC Cuts at: 794 4181 4367 794 Size: 3387 186 3832 Fragments arranged by size: 3832 3387 186 BstBI TT'CG_AA Cuts at: 6209 6209 Size: 7405 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7405 BstXI CCAn_nnnn'nTGG Cuts at: 4042 4396 4042 Size: 354 7051 BstYI r'GATC_y Cuts at: 1972 2218 3046 3073 3462 3916 4404 4435 4443 Size: 246 828 27 389 454 488 31 8 Cuts at: 4443 4735 5102 5487 5504 6956 7042 7054 7400 Size: 292 367 385 17 1452 86 12 346 Cuts at: 7400 1972 Size: 1977 Fragments arranged by size: 1977 1452 828 488 454 389 385 367 346 292 246 86 31 27 17 12 8 BstZ17I GTA'TAC Cuts at: 2685 2685 Size: 7405 BtgI C'CryG_G Cuts at: 513 1065 2004 2779 3999 4476 513 Size: 552 939 775 1220 477 3442 Fragments arranged by size: 3442 1220 939 775 552 477 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6666 Fragments arranged by size: 6666 683 56 BtsI GCAGTG_nn' Cuts at: 3318 3561 4299 4356 4368 4574 4921 5715 5735 Size: 243 738 57 12 206 347 794 20 Cuts at: 5735 3318 Size: 4988 Fragments arranged by size: 4988 794 738 347 243 206 57 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7405 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7370 DraI TTT'AAA Cuts at: 3790 4709 3790 Size: 919 6486 DraIII CAC_nnn'GTG Cuts at: 4570 4570 Size: 7405 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4480 6412 809 Size: 507 3164 1932 1802 Fragments arranged by size: 3164 1932 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2776 4338 4386 Size: 54 1425 50 275 18 946 1562 48 Cuts at: 4386 4549 4852 5741 7370 8 Size: 163 303 889 1629 43 Fragments arranged by size: 1629 1562 1425 946 889 303 275 163 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4549 4852 1537 Size: 275 2737 303 4090 Fragments arranged by size: 4090 2737 303 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2318 5335 1353 Size: 965 3017 3423 Fragments arranged by size: 3423 3017 965 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4587 5992 6366 6512 387 Size: 1064 138 2998 1405 374 146 1280 Fragments arranged by size: 2998 1405 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2041 2263 2367 2837 3227 3269 3933 Size: 393 409 222 104 470 390 42 664 Cuts at: 3933 3950 4502 5468 6065 6852 7120 1239 Size: 17 552 966 597 787 268 1524 Fragments arranged by size: 1524 966 787 664 597 552 470 409 393 390 268 222 104 42 17 EcoNI CCTnn'n_nnAGG Cuts at: 4599 4599 Size: 7405 EcoO109I rG'GnC_Cy Cuts at: 4591 4592 4650 4591 Size: 1 58 7346 Fragments arranged by size: 7346 58 1 EcoRI G'AATT_C Cuts at: 4714 5092 4714 Size: 378 7027 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3652 3652 Size: 7405 FspI TGC'GCA Cuts at: 3893 5911 3893 Size: 2018 5387 HaeII r_GCGC'y Cuts at: 1678 6552 1678 Size: 4874 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2210 2242 2288 2320 3224 Size: 32 135 32 638 32 46 32 904 Cuts at: 3224 3256 3347 3379 4237 4269 6046 6078 6120 Size: 32 91 32 858 32 1777 32 42 Cuts at: 6120 6152 1373 Size: 32 2626 Fragments arranged by size: 2626 1777 904 858 638 135 91 46 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3633 4749 4995 669 Size: 988 281 1695 1116 246 3079 Fragments arranged by size: 3079 1695 1116 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7405 HpaI GTT'AAC Cuts at: 4995 4995 Size: 7405 KpnI G_GTAC'C Cuts at: 4526 4730 4526 Size: 204 7201 MfeI C'AATT_G Cuts at: 5004 5004 Size: 7405 MluI A'CGCG_T Cuts at: 7281 7281 Size: 7405 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2996 6519 6703 1305 Size: 1691 3523 184 2007 Fragments arranged by size: 3523 2007 1691 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2778 4340 10 Size: 54 1425 343 946 1562 3075 Fragments arranged by size: 3075 1562 1425 946 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2094 2595 2613 3335 4254 4361 5363 Size: 648 928 501 18 722 919 107 1002 Cuts at: 5363 5722 5881 518 Size: 359 159 2042 Fragments arranged by size: 2042 1002 928 919 722 648 501 359 159 107 18 MspA1I CmG'CkG Cuts at: 1606 2302 2635 4319 4821 5498 6646 6891 1606 Size: 696 333 1684 502 677 1148 245 2120 Fragments arranged by size: 2120 1684 1148 696 677 502 333 245 NaeI GCC'GGC Cuts at: 4549 4549 Size: 7405 NcoI C'CATG_G Cuts at: 513 1065 2004 2779 4476 513 Size: 552 939 775 1697 3442 Fragments arranged by size: 3442 1697 939 775 552 NdeI CA'TA_TG Cuts at: 387 3157 387 Size: 2770 4635 NgoMIV G'CCGG_C Cuts at: 4547 4547 Size: 7405 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7405 NotI GC'GGCC_GC Cuts at: 4852 4852 Size: 7405 NsiI A_TGCA'T Cuts at: 2091 2846 2091 Size: 755 6650 NspI r_CATG'y Cuts at: 1132 1229 3026 4763 6308 1132 Size: 97 1797 1737 1545 2229 Fragments arranged by size: 2229 1797 1737 1545 97 PasI CC'CwG_GG Cuts at: 1348 4646 1348 Size: 3298 4107 PciI A'CATG_T Cuts at: 6304 6304 Size: 7405 PflMI CCAn_nnn'nTGG Cuts at: 1108 4126 1108 Size: 3018 4387 PfoI T'CCnGG_A Cuts at: 3939 4446 3939 Size: 507 6898 PmeI GTTT'AAAC Cuts at: 4709 4709 Size: 7405 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5474 5506 6987 7019 5474 Size: 32 1481 32 5860 Fragments arranged by size: 5860 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4650 4650 Size: 7405 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7405 PsiI TTA'TAA Cuts at: 3437 4975 3437 Size: 1538 5867 PspOMI G'GGCC_C Cuts at: 4591 4591 Size: 7405 PstI C_TGCA'G Cuts at: 830 2994 4363 4515 4581 4757 830 Size: 2164 1369 152 66 176 3478 Fragments arranged by size: 3478 2164 1369 176 152 66 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7405 PvuII CAG'CTG Cuts at: 2302 2635 2302 Size: 333 7072 SalI G'TCGA_C Cuts at: 1936 4747 1936 Size: 2811 4594 SanDI GG'GwC_CC Cuts at: 4650 4650 Size: 7405 SapI GCTCTTCn'nnn_ Cuts at: 2318 2318 Size: 7405 SbfI CC_TGCA'GG Cuts at: 4757 4757 Size: 7405 ScaI AGT'ACT Cuts at: 5653 7365 5653 Size: 1712 5693 SexAI A'CCwGG_T Cuts at: 4566 4566 Size: 7405 SfcI C'TryA_G Cuts at: 826 1045 2390 2990 3217 4359 4511 4577 4753 Size: 219 1345 600 227 1142 152 66 176 Cuts at: 4753 5888 6569 6760 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1345 1142 1135 681 600 227 219 191 176 152 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7405 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7405 SmaI CCC'GGG Cuts at: 1872 4732 1872 Size: 2860 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3169 3386 4529 5509 6410 6672 Size: 197 926 1226 217 1143 980 901 262 Cuts at: 6672 820 Size: 1553 Fragments arranged by size: 1553 1226 1143 980 926 901 262 217 197 SnaBI TAC'GTA Cuts at: 493 5196 493 Size: 4703 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7405 SphI G_CATG'C Cuts at: 1132 4763 1132 Size: 3631 3774 SspI AAT'ATT Cuts at: 5 52 3178 5329 5 Size: 47 3126 2151 2081 Fragments arranged by size: 3126 2151 2081 47 StuI AGG'CCT Cuts at: 1517 4586 1517 Size: 3069 4336 StyI C'CwwG_G Cuts at: 513 1065 2004 2779 3742 4093 4241 4476 4485 Size: 552 939 775 963 351 148 235 9 Cuts at: 4485 4844 513 Size: 359 3074 Fragments arranged by size: 3074 963 939 775 552 359 351 235 148 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2714 3774 4679 5432 5449 5602 5787 Size: 1255 1134 1060 905 753 17 153 185 Cuts at: 5787 6232 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1134 1060 905 753 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2129 2176 4146 Size: 274 80 33 51 1443 152 47 1970 Cuts at: 4146 5651 7363 96 Size: 1505 1712 138 Fragments arranged by size: 1970 1712 1505 1443 274 152 138 80 51 47 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2733 2765 4554 4586 4606 4638 7365 7397 2733 Size: 32 1789 32 20 32 2727 32 2741 Fragments arranged by size: 2741 2727 1789 32 32 32 32 20 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7405 XbaI T'CTAG_A Cuts at: 4741 4741 Size: 7405 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2771 3598 4483 4633 2771 Size: 827 885 150 5543 Fragments arranged by size: 5543 885 827 150 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7405 XmaI C'CCGG_G Cuts at: 1870 4730 1870 Size: 2860 4545 XmnI GAAnn'nnTTC Cuts at: 3429 3718 3935 5534 3429 Size: 289 217 1599 5300 Fragments arranged by size: 5300 1599 289 217 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3363 5213 276 Size: 53 83 186 936 1829 1850 2468 Fragments arranged by size: 2468 1850 1829 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BsiWI Bsu36I EcoICRI EcoRV FseI FspAI KasI NarI NruI PacI PmlI PspXI PsrI RsrII SacI SacII SfiI SfoI SrfI SwaI