################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AccI AclI AflII AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I AspEI AspI AvrII BamHI BbrPI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsiWI BsmI BspDI BspLU11I BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KspI MamI MfeI MluI MseI MunI NaeI NcoI NgoMIV NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RsrII SacII SalI SbfI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 135 GACGTC GACGT^C Acc65I 1668 GGTACC G^GTACC AccIII 636 TCCGGA T^CCGGA AcsI 567 AAATTT [GA]^AATT[CT] Alw44I 1510 GTGCAC G^TGCAC AocI 715 CCTGAGG CC^T[ACGT]AGG ApaLI 1510 GTGCAC G^TGCAC ApoI 567 AAATTT [GA]^AATT[CT] Asp718I 1668 GGTACC G^GTACC AviII 1127 TGCGCA TGC^GCA BbeI 149 GGCGCC GGCGC^C BbvCI 1579 CCTCAGC CCTCAGC(-5/-2) BcgI 89 CGAGTGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1589 GTATCC GTATCC(6/5) BfaI 1019 CTAG C^TAG BglII 1641 AGATCT A^GATCT BseAI 636 TCCGGA T^CCGGA BseRI 1717 CTCCTC GAGGAG(10/8) BsgI 337 CTGCAC GTGCAG(16/14) Bsp1407I 749 TGTACA T^GTACA BspEI 636 TCCGGA T^CCGGA BspHI 1628 TCATGA T^CATGA BsrFI 13 GCCGGT [GA]^CCGG[CT] BsrGI 749 TGTACA T^GTACA Bsu36I 715 CCTGAGG CC^T[ACGT]AGG Cfr10I 13 GCCGGT [GA]^CCGG[CT] Eco81I 715 CCTGAGG CC^T[ACGT]AGG EcoNI 1626 CCTCATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 944 CCAAGG C^C[AT][AT]GG FspI 1127 TGCGCA TGC^GCA HincII 1402 GTTGAC GT[CT]^[GA]AC HindII 1402 GTTGAC GT[CT]^[GA]AC KasI 149 GGCGCC G^GCGCC KpnI 1668 GGTACC GGTAC^C MaeI 1019 CTAG C^TAG MroI 636 TCCGGA T^CCGGA NarI 149 GGCGCC GG^CGCC NdeI 1480 CATATG CA^TATG PpuMI 551 GGGACCT [GA]G^G[AT]CC[CT] PvuI 960 CGATCG CGAT^CG RcaI 1628 TCATGA T^CATGA SacI 418 GAGCTC GAGCT^C SapI 1461 GCTCTTC GCTCTTC(1/4) ScaI 521 AGTACT AGT^ACT SfoI 149 GGCGCC GGC^GCC SspBI 749 TGTACA T^GTACA StyI 944 CCAAGG C^C[AT][AT]GG TaqI 963 TCGA T^CGA TthHB8I 963 TCGA T^CGA XcmI 1442 CCAGCCGCCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcyI 135 GACGTC G[GA]^CG[CT]C 149 GGCGCC ApaI 73 GGGCCC GGGCC^C 1568 GGGCCC BalI 1140 TGGCCA TGG^CCA 1280 TGGCCA BbsI 651 GAAGAC GAAGAC(2/6) 1216 GAAGAC BpuAI 651 GAAGAC GAAGAC(2/6) 1216 GAAGAC BsaHI 135 GACGTC G[GA]^CG[CT]C 149 GGCGCC BsaWI 636 TCCGGA [AT]^CCGG[AT] 1105 TCCGGT BsmBI 781 GAGACG CGTCTC(1/5) 799 GAGACG BsrBI 59 CCGCTC CCGCTC(-3/-3) 1116 GAGCGG BstXI 671 CCAACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1283 CCACAGTAGTGG DraII 551 GGGACCT [GA]G^G[ACGT]CC[CT] 1568 GGGCCCT DraIII 361 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG 1226 CACCCCGTG EcoO109I 551 GGGACCT [GA]G^G[ACGT]CC[CT] 1568 GGGCCCT HaeII 149 GGCGCC [GA]GCGC^[CT] 256 AGCGCC HgaI 391 GACGC GACGC(5/10) 290 GCGTC Hin1I 135 GACGTC G[GA]^CG[CT]C 149 GGCGCC MaeII 136 ACGT A^CGT 802 ACGT MluNI 1140 TGGCCA TGG^CCA 1280 TGGCCA MscI 1140 TGGCCA TGG^CCA 1280 TGGCCA NotI 46 GCGGCCGC GC^GGCCGC 173 GCGGCCGC SfiI 1356 GGCCCCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1485 GGCCCTGTCGGCC SmlI 517 CTCAAG C^T[CT][GA]AG 556 CTCAAG TfiI 1077 GAATC G^A[AT]TC 1779 GATTC Tsp509I 568 AATT ^AATT 1318 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbvI BcnI BglI BmyI BpmI BsaI BsaJI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EagI EarI EclXI Eco52I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI TseI Tsp45I TspRI XhoII XmaCI XmaI