################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI AocI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BbrPI BbsI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BsiWI BsmBI BspDI BspHI BspLU11I BsrDI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MseI MunI NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tru9I Tth111I Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AccI 1021 GTCTAC GT^[AC][GT]AC AcsI 1000 GAATTC [GA]^AATT[CT] AflIII 1412 ACACGT A^C[GA][CT]GT Alw44I 1558 GTGCAC G^TGCAC ApaLI 1558 GTGCAC G^TGCAC ApoI 1000 GAATTC [GA]^AATT[CT] BbeI 282 GGCGCC GGCGC^C BglI 1271 GCCAGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 543 GCTGAGC GC^T[ACGT]AGC Bpu1102I 543 GCTGAGC GC^T[ACGT]AGC BsaAI 1413 CACGTA [CT]AC^GT[GA] Bsp1407I 1775 TGTACA T^GTACA BsrFI 1157 ACCGGC [GA]^CCGG[CT] BsrGI 1775 TGTACA T^GTACA CelII 543 GCTGAGC GC^T[ACGT]AGC Cfr10I 1157 ACCGGC [GA]^CCGG[CT] DraIII 1387 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 558 CCTGACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1000 GAATTC G^AATTC EcoT14I 1919 CCAAGG C^C[AT][AT]GG KasI 282 GGCGCC G^GCGCC NarI 282 GGCGCC GG^CGCC NotI 1650 GCGGCCGC GC^GGCCGC PvuII 1405 CAGCTG CAG^CTG ScaI 392 AGTACT AGT^ACT SexAI 1418 ACCTGGT A^CC[AT]GGT SfoI 282 GGCGCC GGC^GCC SspBI 1775 TGTACA T^GTACA StuI 1892 AGGCCT AGG^CCT StyI 1919 CCAAGG C^C[AT][AT]GG Tsp509I 1001 AATT ^AATT TspRI 2069 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccIII 884 TCCGGA T^CCGGA 2033 TCCGGA Aor51HI 254 AGCGCT AGC^GCT 1186 AGCGCT AviII 200 TGCGCA TGC^GCA 1508 TGCGCA BamHI 758 GGATCC G^GATCC 837 GGATCC BbvCI 406 GCTGAGG CCTCAGC(-5/-2) 742 GCTGAGG BcgI 1335 CGAAGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2068 GCAGTGCGCTCG BciVI 1072 GGATAC GTATCC(6/5) 1906 GTATCC BsaWI 884 TCCGGA [AT]^CCGG[AT] 2033 TCCGGA BseAI 884 TCCGGA T^CCGGA 2033 TCCGGA BsmI 2148 GAATGC GAATGC(1/-1) 2101 GCATTC BspEI 884 TCCGGA T^CCGGA 2033 TCCGGA BstXI 1919 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2090 CCACCTTCATGG EarI 682 CTCTTC CTCTTC(1/4) 2136 GAAGAG Eco47III 254 AGCGCT AGC^GCT 1186 AGCGCT FspI 200 TGCGCA TGC^GCA 1508 TGCGCA Ksp632I 682 CTCTTC CTCTTC(1/4) 2136 GAAGAG MaeIII 295 GTCAC ^GT[ACGT]AC 1362 GTCAC MroI 884 TCCGGA T^CCGGA 2033 TCCGGA MslI 189 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2091 CACCTTCATG PaeR7I 1972 CTCGAG C^TCGAG 2050 CTCGAG SapI 2136 GAAGAGC GCTCTTC(1/4) 681 GCTCTTC Tsp45I 295 GTCAC ^GT[GC]AC 1362 GTCAC XcmI 599 CCATCGAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1806 CCAGGGGGCCTCTGG XhoI 1972 CTCGAG C^TCGAG 2050 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssHII BssKI BssSI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI TaqI TfiI TseI TthHB8I XhoII XmaCI XmaI