(Circular) (Six-base) MAPSORT of: pFN21AE2091.seq Check: 7696 from: 1 to: 7000 pFN21AE2091 7000 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2466 2466 Size: 7000 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4810 278 Size: 53 83 186 936 3274 2468 Fragments arranged by size: 3274 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3027 4343 1937 Size: 1090 1316 4594 Fragments arranged by size: 4594 1316 1090 Acc65I G'GTAC_C Cuts at: 4321 4321 Size: 7000 AclI AA'CG_TT Cuts at: 1563 5127 5500 1563 Size: 3564 373 3063 Fragments arranged by size: 3564 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2789 3469 3992 4160 5063 6447 6715 1632 Size: 1157 680 523 168 903 1384 268 1917 Fragments arranged by size: 1917 1384 1157 903 680 523 268 168 AfeI AGC'GCT Cuts at: 2261 3193 2261 Size: 932 6068 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6803 AflIII A'CryG_T Cuts at: 1203 3417 5899 6876 1203 Size: 2214 2482 977 1327 Fragments arranged by size: 2482 2214 1327 977 AgeI A'CCGG_T Cuts at: 5831 5831 Size: 7000 AhdI GACnn_n'nnGTC Cuts at: 5729 5729 Size: 7000 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7000 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3726 1184 Size: 2542 4458 AlwNI CAG_nnn'CTG Cuts at: 2818 2830 3511 4186 4743 6315 2818 Size: 12 681 675 557 1572 3503 Fragments arranged by size: 3503 1572 681 675 557 12 ApaI G_GGCC'C Cuts at: 2805 2871 3619 4071 4267 2805 Size: 66 748 452 196 5538 Fragments arranged by size: 5538 748 452 196 66 ApaLI G'TGCA_C Cuts at: 3563 5057 6213 3563 Size: 1494 1156 4350 Fragments arranged by size: 4350 1494 1156 ApoI r'AATT_y Cuts at: 1493 3005 4309 4535 4687 6796 1493 Size: 1512 1304 226 152 2109 1697 Fragments arranged by size: 2109 1697 1512 1304 226 152 AseI AT'TA_AT Cuts at: 160 1032 5554 160 Size: 872 4522 1606 Fragments arranged by size: 4522 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7000 AvaI C'yCGr_G Cuts at: 1870 1943 3611 3668 3716 3977 4055 4081 4234 Size: 73 1668 57 48 261 78 26 153 Cuts at: 4234 4325 1870 Size: 91 4545 Fragments arranged by size: 4545 1668 261 153 91 78 73 57 48 26 BamHI G'GATC_C Cuts at: 2763 2842 4330 4697 2763 Size: 79 1488 367 5066 Fragments arranged by size: 5066 1488 367 79 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2287 2600 3091 3673 4321 Size: 325 217 627 500 313 491 582 648 Cuts at: 4321 5776 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 648 627 582 500 491 325 313 217 BanII G_rGCy'C Cuts at: 1391 2805 2871 3619 3945 4071 4217 4246 4267 Size: 1414 66 748 326 126 146 29 21 Cuts at: 4267 4319 1391 Size: 52 4072 Fragments arranged by size: 4072 1414 748 326 146 126 66 52 29 21 BbeI G_GCGC'C Cuts at: 2291 2291 Size: 7000 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6029 BbvCI CC'TCA_GC Cuts at: 2412 2748 2412 Size: 336 6664 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3329 3363 4062 4096 5191 5225 1903 Size: 34 1392 34 699 34 1095 34 3678 Fragments arranged by size: 3678 1392 1095 699 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3071 3922 4892 6102 3071 Size: 851 970 1210 3969 Fragments arranged by size: 3969 1210 970 851 BclI T'GATC_A Cuts at: 1548 1548 Size: 7000 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3282 5611 136 Size: 107 122 71 631 605 1610 2329 1525 Fragments arranged by size: 2329 1610 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6995 6995 Size: 7000 BlpI GC'TnA_GC Cuts at: 2549 4417 2549 Size: 1868 5132 Bme1580I G_kGCm'C Cuts at: 1790 2076 2126 2172 2805 2871 2881 3567 3619 Size: 286 50 46 633 66 10 686 52 Cuts at: 3619 3678 4071 4267 5061 6217 1790 Size: 59 393 196 794 1156 2573 Fragments arranged by size: 2573 1156 794 686 633 393 286 196 66 59 52 50 46 10 BmgBI CAC'GTC Cuts at: 1318 4051 1318 Size: 2733 4267 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4731 5689 442 Size: 416 53 83 399 24 3314 958 1753 Fragments arranged by size: 3314 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7000 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2291 2516 2744 3038 3177 3341 3452 3479 Size: 1052 225 228 294 139 164 111 27 Cuts at: 3479 3781 5660 1239 Size: 302 1879 2579 Fragments arranged by size: 2579 1879 1052 302 294 228 225 164 139 111 27 Bpu10I CC'TnA_GC Cuts at: 2412 2748 2412 Size: 336 6664 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2096 3560 5125 5990 6288 2096 Size: 1464 1565 865 298 2808 Fragments arranged by size: 2808 1565 1464 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2478 3153 4091 5663 882 Size: 617 979 675 938 1572 2219 Fragments arranged by size: 2219 1572 979 938 675 617 BsaAI yAC'GTr Cuts at: 493 1204 3420 4791 493 Size: 711 2216 1371 2702 Fragments arranged by size: 2702 2216 1371 711 BsaBI GATnn'nnATC Cuts at: 6994 6994 Size: 7000 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2288 3686 3771 4043 Size: 53 83 186 936 755 1398 85 272 Cuts at: 4043 4807 5189 5824 275 Size: 764 382 635 1451 Fragments arranged by size: 1451 1398 936 764 755 635 382 272 186 85 83 53 BsaWI w'CCGG_w Cuts at: 2889 4038 5433 5831 6105 6252 6663 2889 Size: 1149 1395 398 274 147 411 3226 Fragments arranged by size: 3226 1395 1149 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6775 Fragments arranged by size: 6775 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 2585 3563 4015 1186 Size: 907 492 978 452 4171 Fragments arranged by size: 4171 978 907 492 452 BseYI C'CCAG_C Cuts at: 2119 3232 6203 6699 2119 Size: 1113 2971 496 2420 Fragments arranged by size: 2971 2420 1113 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2243 2291 2321 2444 3374 3398 4206 Size: 573 507 48 30 123 930 24 808 Cuts at: 4206 1163 Size: 3957 Fragments arranged by size: 3957 930 808 573 507 123 48 30 24 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3531 3659 3849 4450 5211 6239 Size: 275 186 1530 128 190 601 761 1028 Cuts at: 6239 1540 Size: 2301 Fragments arranged by size: 2301 1530 1028 761 601 275 190 186 128 BsiHKAI G_wGCw'C Cuts at: 2247 2777 3465 3567 4056 5061 5146 6217 2247 Size: 530 688 102 489 1005 85 1071 3030 Fragments arranged by size: 3030 1071 1005 688 530 489 102 85 BsmI GAATG_Cn' Cuts at: 4106 4159 4511 4604 4106 Size: 53 352 93 6502 Fragments arranged by size: 6502 352 93 53 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2126 2172 2247 2777 2805 2871 Size: 399 286 50 46 75 530 28 66 Cuts at: 2871 2881 3465 3567 3619 3678 3945 4056 4071 Size: 10 584 102 52 59 267 111 15 Cuts at: 4071 4217 4246 4267 4319 5061 5146 6217 1391 Size: 146 29 21 52 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 584 530 399 286 267 146 111 102 85 75 66 59 52 52 50 46 29 28 21 15 10 BspEI T'CCGG_A Cuts at: 2889 4038 6663 2889 Size: 1149 2625 3226 Fragments arranged by size: 3226 2625 1149 BspHI T'CATG_A Cuts at: 4889 6619 4889 Size: 1730 5270 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2466 2826 3225 3790 4355 844 Size: 1622 360 399 565 565 3489 Fragments arranged by size: 3489 1622 565 565 399 360 BsrBI CCG'CTC Cuts at: 1394 1823 2361 2581 2973 3282 4036 4887 1394 Size: 429 538 220 392 309 754 851 3507 Fragments arranged by size: 3507 851 754 538 429 392 309 220 BsrDI GCAATG_nn' Cuts at: 65 1248 5495 5669 65 Size: 1183 4247 174 1396 Fragments arranged by size: 4247 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3162 5644 5831 1700 Size: 110 1352 2482 187 2869 Fragments arranged by size: 2869 2482 1352 187 110 BsrGI T'GTAC_A Cuts at: 96 3780 96 Size: 3684 3316 BssHII G'CGCG_C Cuts at: 1925 2227 4017 4019 1925 Size: 302 1790 2 4906 Fragments arranged by size: 4906 1790 302 2 BssSI C'ACGA_G Cuts at: 2075 3392 3521 3545 3997 5060 6072 2075 Size: 1317 129 24 452 1063 1012 3003 Fragments arranged by size: 3003 1317 1063 1012 452 129 24 BstAPI GCAn_nnn'nTGC Cuts at: 794 2818 2830 3397 4186 794 Size: 2024 12 567 789 3608 Fragments arranged by size: 3608 2024 789 567 12 BstBI TT'CG_AA Cuts at: 5804 5804 Size: 7000 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7000 BstXI CCAn_nnnn'nTGG Cuts at: 3931 4102 3931 Size: 171 6829 BstYI r'GATC_y Cuts at: 1972 2763 2842 3303 3507 4330 4697 5082 5099 Size: 791 79 461 204 823 367 385 17 Cuts at: 5099 6551 6637 6649 6995 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 823 791 461 385 367 346 204 86 79 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2037 2805 3951 513 Size: 552 939 33 768 1146 3562 Fragments arranged by size: 3562 1146 939 768 552 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6261 Fragments arranged by size: 6261 683 56 BtsI GCAGTG_nn' Cuts at: 4080 4516 5310 5330 4080 Size: 436 794 20 5750 Fragments arranged by size: 5750 794 436 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7000 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6965 DraI TTT'AAA Cuts at: 4304 4304 Size: 7000 DraIII CAC_nnn'GTG Cuts at: 3397 3397 Size: 7000 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6007 809 Size: 507 4691 1802 Fragments arranged by size: 4691 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3164 3183 3283 Size: 54 1425 50 275 18 1334 19 100 Cuts at: 3283 3528 3656 3823 3846 4164 4447 5336 6965 Size: 245 128 167 23 318 283 889 1629 Cuts at: 6965 8 Size: 43 Fragments arranged by size: 1629 1425 1334 889 318 283 275 245 167 128 100 54 50 43 23 19 18 EagI C'GGCC_G Cuts at: 1537 1812 3528 3656 3846 4447 1537 Size: 275 1716 128 190 601 4090 Fragments arranged by size: 4090 1716 601 275 190 128 EarI CTCTTCn'nnn_ Cuts at: 1353 2693 4136 4930 1353 Size: 1340 1443 794 3423 Fragments arranged by size: 3423 1443 1340 794 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4189 5587 5961 6107 387 Size: 1064 138 2600 1398 374 146 1280 Fragments arranged by size: 2600 1398 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2291 2516 2744 2789 3038 3177 3341 Size: 393 659 225 228 45 249 139 164 Cuts at: 3341 3452 3469 3479 3781 3992 4160 5063 5660 Size: 111 17 10 302 211 168 903 597 Cuts at: 5660 6447 6715 1239 Size: 787 268 1524 Fragments arranged by size: 1524 903 787 659 597 393 302 268 249 228 225 211 168 164 139 111 45 17 10 EcoNI CCTnn'n_nnAGG Cuts at: 2567 2567 Size: 7000 EcoO109I rG'GnC_Cy Cuts at: 2706 2815 2867 2868 2911 3058 3615 3616 3802 Size: 109 52 1 43 147 557 1 186 Cuts at: 3802 3816 4263 4264 2706 Size: 14 447 1 5442 Fragments arranged by size: 5442 557 447 186 147 109 52 43 14 1 1 1 EcoRI G'AATT_C Cuts at: 3005 4309 4687 3005 Size: 1304 378 5318 Fragments arranged by size: 5318 1304 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3499 3499 Size: 7000 FspI TGC'GCA Cuts at: 2207 3515 5506 2207 Size: 1308 1991 3701 Fragments arranged by size: 3701 1991 1308 HaeII r_GCGC'y Cuts at: 1678 2219 2263 2291 2395 3149 3195 3277 3880 Size: 541 44 28 104 754 46 82 603 Cuts at: 3880 4197 6147 1678 Size: 317 1950 2531 Fragments arranged by size: 2531 1950 754 603 541 317 104 82 46 44 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3295 3327 4233 4265 5641 Size: 32 135 32 1723 32 906 32 1376 Cuts at: 5641 5673 5715 5747 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1723 1376 906 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4344 4590 669 Size: 988 281 2406 246 3079 Fragments arranged by size: 3079 2406 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7000 HpaI GTT'AAC Cuts at: 4590 4590 Size: 7000 KasI G'GCGC_C Cuts at: 2287 2287 Size: 7000 KpnI G_GTAC'C Cuts at: 4325 4325 Size: 7000 MfeI C'AATT_G Cuts at: 4599 4599 Size: 7000 MluI A'CGCG_T Cuts at: 6876 6876 Size: 7000 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3474 6114 6298 1305 Size: 2169 2640 184 2007 Fragments arranged by size: 2640 2169 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3185 3825 4166 10 Size: 54 1425 343 1353 640 341 2844 Fragments arranged by size: 2844 1425 1353 640 343 341 54 MslI CAynn'nnrTG Cuts at: 518 1166 2198 4100 4958 5317 5476 518 Size: 648 1032 1902 858 359 159 2042 Fragments arranged by size: 2042 1902 1032 858 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2827 3179 3264 3412 4416 5093 6241 6486 Size: 1221 352 85 148 1004 677 1148 245 Cuts at: 6486 1606 Size: 2120 Fragments arranged by size: 2120 1221 1148 1004 677 352 245 148 85 NarI GG'CG_CC Cuts at: 2288 2288 Size: 7000 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5509 Fragments arranged by size: 5509 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7000 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7000 NotI GC'GGCC_GC Cuts at: 3656 4447 3656 Size: 791 6209 NspI r_CATG'y Cuts at: 1132 1229 4358 5903 1132 Size: 97 3129 1545 2229 Fragments arranged by size: 3129 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2872 1348 Size: 1524 5476 PciI A'CATG_T Cuts at: 5899 5899 Size: 7000 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7000 PfoI T'CCnGG_A Cuts at: 2346 3429 2346 Size: 1083 5917 PmeI GTTT'AAAC Cuts at: 4304 4304 Size: 7000 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5069 5101 6582 6614 5069 Size: 32 1481 32 5455 Fragments arranged by size: 5455 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2815 3058 3802 2815 Size: 243 744 6013 Fragments arranged by size: 6013 744 243 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7000 PsiI TTA'TAA Cuts at: 4570 4570 Size: 7000 PspOMI G'GGCC_C Cuts at: 2801 2867 3615 4067 4263 2801 Size: 66 748 452 196 5538 Fragments arranged by size: 5538 748 452 196 66 PspXI vC'TCGA_Gb Cuts at: 3977 4055 3977 Size: 78 6922 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3578 3610 3578 Size: 32 6968 PstI C_TGCA'G Cuts at: 830 2826 3705 4008 4352 830 Size: 1996 879 303 344 3478 Fragments arranged by size: 3478 1996 879 344 303 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7000 PvuII CAG'CTG Cuts at: 3412 3412 Size: 7000 SalI G'TCGA_C Cuts at: 1936 4342 1936 Size: 2406 4594 SanDI GG'GwC_CC Cuts at: 3058 3058 Size: 7000 SapI GCTCTTCn'nnn_ Cuts at: 2693 4136 2693 Size: 1443 5557 SbfI CC_TGCA'GG Cuts at: 4352 4352 Size: 7000 ScaI AGT'ACT Cuts at: 2399 5248 6960 2399 Size: 2849 1712 2439 Fragments arranged by size: 2849 2439 1712 SexAI A'CCwGG_T Cuts at: 3423 3423 Size: 7000 SfcI C'TryA_G Cuts at: 826 1045 2822 3701 4004 4348 5483 6164 6355 Size: 219 1777 879 303 344 1135 681 191 Cuts at: 6355 826 Size: 1471 Fragments arranged by size: 1777 1471 1135 879 681 344 303 219 191 SfoI GGC'GCC Cuts at: 2289 2289 Size: 7000 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7000 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7000 SmaI CCC'GGG Cuts at: 1872 3613 3670 4236 4327 1872 Size: 1741 57 566 91 4545 Fragments arranged by size: 4545 1741 566 91 57 SmlI C'TyrA_G Cuts at: 820 1017 1943 2111 3575 3977 4055 5104 6005 Size: 197 926 168 1464 402 78 1049 901 Cuts at: 6005 6267 820 Size: 262 1553 Fragments arranged by size: 1553 1464 1049 926 901 402 262 197 168 78 SnaBI TAC'GTA Cuts at: 493 4791 493 Size: 4298 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7000 SphI G_CATG'C Cuts at: 1132 4358 1132 Size: 3226 3774 SspI AAT'ATT Cuts at: 5 52 4924 5 Size: 47 4872 2081 Fragments arranged by size: 4872 2081 47 StuI AGG'CCT Cuts at: 1517 3899 1517 Size: 2382 4618 StyI C'CwwG_G Cuts at: 513 1065 2004 3924 4439 513 Size: 552 939 1920 515 3074 Fragments arranged by size: 3074 1920 939 552 515 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2393 2911 3146 5027 5044 5197 5382 Size: 1255 813 518 235 1881 17 153 185 Cuts at: 5382 5827 325 Size: 445 1498 Fragments arranged by size: 1881 1498 1255 813 518 445 235 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2397 2610 3780 Size: 274 80 33 51 1443 420 213 1170 Cuts at: 3780 5246 6958 96 Size: 1466 1712 138 Fragments arranged by size: 1712 1466 1443 1170 420 274 213 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2871 2903 6960 6992 2871 Size: 32 4057 32 2879 Fragments arranged by size: 4057 2879 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7000 XbaI T'CTAG_A Cuts at: 4336 4336 Size: 7000 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2611 3818 2611 Size: 1207 5793 XhoI C'TCGA_G Cuts at: 1943 3977 4055 1943 Size: 2034 78 4888 Fragments arranged by size: 4888 2034 78 XmaI C'CCGG_G Cuts at: 1870 3611 3668 4234 4325 1870 Size: 1741 57 566 91 4545 Fragments arranged by size: 4545 1741 566 91 57 XmnI GAAnn'nnTTC Cuts at: 5129 5129 Size: 7000 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4808 276 Size: 53 83 186 936 3274 2468 Fragments arranged by size: 3274 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI RsrII SacI SacII SfiI SrfI SwaI