(Linear) (Six-base) MAPSORT of: KIEE2091.seq Check: 4030 from: 1 to: 2295 KIEE2091.orf With 182 enzymes: SgfI * July 3, 2012 17:08 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 461 2295 Size: 461 1834 AccI GT'mk_AC Cuts at: 0 1022 2295 Size: 1022 1273 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 784 1464 1987 2155 2295 Size: 784 680 523 168 140 Fragments arranged by size: 784 680 523 168 140 AfeI AGC'GCT Cuts at: 0 256 1188 2295 Size: 256 932 1107 Fragments arranged by size: 1107 932 256 AflIII A'CryG_T Cuts at: 0 1412 2295 Size: 1412 883 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1721 2295 Size: 1721 574 AlwNI CAG_nnn'CTG Cuts at: 0 813 825 1506 2181 2295 Size: 813 12 681 675 114 Fragments arranged by size: 813 681 675 114 12 ApaI G_GGCC'C Cuts at: 0 800 866 1614 2066 2262 2295 Size: 800 66 748 452 196 33 Fragments arranged by size: 800 748 452 196 66 33 ApaLI G'TGCA_C Cuts at: 0 1558 2295 Size: 1558 737 ApoI r'AATT_y Cuts at: 0 1000 2295 Size: 1000 1295 AvaI C'yCGr_G Cuts at: 0 1606 1663 1711 1972 2050 2076 2229 2295 Size: 1606 57 48 261 78 26 153 66 Fragments arranged by size: 1606 261 153 78 66 57 48 26 BamHI G'GATC_C Cuts at: 0 758 837 2295 Size: 758 79 1458 Fragments arranged by size: 1458 758 79 BanI G'GyrC_C Cuts at: 0 282 595 1086 1668 2295 Size: 282 313 491 582 627 Fragments arranged by size: 627 582 491 313 282 BanII G_rGCy'C Cuts at: 0 800 866 1614 1940 2066 2212 2241 2262 Size: 800 66 748 326 126 146 29 21 Cuts at: 2262 2295 Size: 33 Fragments arranged by size: 800 748 326 146 126 66 33 29 21 BbeI G_GCGC'C Cuts at: 0 286 2295 Size: 286 2009 BbvCI CC'TCA_GC Cuts at: 0 407 743 2295 Size: 407 336 1552 Fragments arranged by size: 1552 407 336 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1324 1358 2057 2091 2295 Size: 1324 34 699 34 204 Fragments arranged by size: 1324 699 204 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1066 1917 2295 Size: 1066 851 378 Fragments arranged by size: 1066 851 378 BglI GCCn_nnn'nGGC Cuts at: 0 1277 2295 Size: 1277 1018 BlpI GC'TnA_GC Cuts at: 0 544 2295 Size: 544 1751 Bme1580I G_kGCm'C Cuts at: 0 71 121 167 800 866 876 1562 1614 Size: 71 50 46 633 66 10 686 52 Cuts at: 1614 1673 2066 2262 2295 Size: 59 393 196 33 Fragments arranged by size: 686 633 393 196 71 66 59 52 50 46 33 10 BmgBI CAC'GTC Cuts at: 0 2046 2295 Size: 2046 249 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 286 511 739 1033 1172 1336 1447 1474 Size: 286 225 228 294 139 164 111 27 Cuts at: 1474 1776 2295 Size: 302 519 Fragments arranged by size: 519 302 294 286 228 225 164 139 111 27 Bpu10I CC'TnA_GC Cuts at: 0 407 743 2295 Size: 407 336 1552 Fragments arranged by size: 1552 407 336 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 91 1555 2295 Size: 91 1464 740 Fragments arranged by size: 1464 740 91 BsaI GGTCTCn'nnnn_ Cuts at: 0 473 1148 2086 2295 Size: 473 675 938 209 Fragments arranged by size: 938 675 473 209 BsaAI yAC'GTr Cuts at: 0 1415 2295 Size: 1415 880 BsaHI Gr'CG_yC Cuts at: 0 283 1681 1766 2038 2295 Size: 283 1398 85 272 257 Fragments arranged by size: 1398 283 272 257 85 BsaWI w'CCGG_w Cuts at: 0 884 2033 2295 Size: 884 1149 262 Fragments arranged by size: 1149 884 262 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 88 580 1558 2010 2295 Size: 88 492 978 452 285 Fragments arranged by size: 978 492 452 285 88 BseYI C'CCAG_C Cuts at: 0 114 1227 2295 Size: 114 1113 1068 Fragments arranged by size: 1113 1068 114 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 238 286 316 439 1369 1393 2201 2295 Size: 238 48 30 123 930 24 808 94 Fragments arranged by size: 930 808 238 123 94 48 30 24 BsiEI CG_ry'CG Cuts at: 0 1526 1654 1844 2295 Size: 1526 128 190 451 Fragments arranged by size: 1526 451 190 128 BsiHKAI G_wGCw'C Cuts at: 0 242 772 1460 1562 2051 2295 Size: 242 530 688 102 489 244 Fragments arranged by size: 688 530 489 244 242 102 BsmI GAATG_Cn' Cuts at: 0 2101 2154 2295 Size: 2101 53 141 Fragments arranged by size: 2101 141 53 Bsp1286I G_dGCh'C Cuts at: 0 71 121 167 242 772 800 866 876 Size: 71 50 46 75 530 28 66 10 Cuts at: 876 1460 1562 1614 1673 1940 2051 2066 2212 Size: 584 102 52 59 267 111 15 146 Cuts at: 2212 2241 2262 2295 Size: 29 21 33 Fragments arranged by size: 584 530 267 146 111 102 75 71 66 59 52 50 46 33 29 28 21 15 10 BspEI T'CCGG_A Cuts at: 0 884 2033 2295 Size: 884 1149 262 Fragments arranged by size: 1149 884 262 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 461 821 1220 1785 2295 Size: 461 360 399 565 510 Fragments arranged by size: 565 510 461 399 360 BsrBI CCG'CTC Cuts at: 0 356 576 968 1277 2031 2295 Size: 356 220 392 309 754 264 Fragments arranged by size: 754 392 356 309 264 220 BsrFI r'CCGG_y Cuts at: 0 1157 2295 Size: 1157 1138 BsrGI T'GTAC_A Cuts at: 0 1775 2295 Size: 1775 520 BssHII G'CGCG_C Cuts at: 0 222 2012 2014 2295 Size: 222 1790 2 281 Fragments arranged by size: 1790 281 222 2 BssSI C'ACGA_G Cuts at: 0 70 1387 1516 1540 1992 2295 Size: 70 1317 129 24 452 303 Fragments arranged by size: 1317 452 303 129 70 24 BstAPI GCAn_nnn'nTGC Cuts at: 0 813 825 1392 2181 2295 Size: 813 12 567 789 114 Fragments arranged by size: 813 789 567 114 12 BstXI CCAn_nnnn'nTGG Cuts at: 0 1926 2097 2295 Size: 1926 171 198 Fragments arranged by size: 1926 198 171 BstYI r'GATC_y Cuts at: 0 758 837 1298 1502 2295 Size: 758 79 461 204 793 Fragments arranged by size: 793 758 461 204 79 BtgI C'CryG_G Cuts at: 0 32 800 1946 2295 Size: 32 768 1146 349 Fragments arranged by size: 1146 768 349 32 BtsI GCAGTG_nn' Cuts at: 0 2075 2295 Size: 2075 220 DraIII CAC_nnn'GTG Cuts at: 0 1392 2295 Size: 1392 903 EaeI y'GGCC_r Cuts at: 0 1159 1178 1278 1523 1651 1818 1841 2159 Size: 1159 19 100 245 128 167 23 318 Cuts at: 2159 2295 Size: 136 Fragments arranged by size: 1159 318 245 167 136 128 100 23 19 EagI C'GGCC_G Cuts at: 0 1523 1651 1841 2295 Size: 1523 128 190 454 Fragments arranged by size: 1523 454 190 128 EarI CTCTTCn'nnn_ Cuts at: 0 688 2131 2295 Size: 688 1443 164 Fragments arranged by size: 1443 688 164 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2184 2295 Size: 2184 111 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 286 511 739 784 1033 1172 1336 1447 Size: 286 225 228 45 249 139 164 111 Cuts at: 1447 1464 1474 1776 1987 2155 2295 Size: 17 10 302 211 168 140 Fragments arranged by size: 302 286 249 228 225 211 168 164 140 139 111 45 17 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 562 2295 Size: 562 1733 EcoO109I rG'GnC_Cy Cuts at: 0 701 810 862 863 906 1053 1610 1611 Size: 701 109 52 1 43 147 557 1 Cuts at: 1611 1797 1811 2258 2259 2295 Size: 186 14 447 1 36 Fragments arranged by size: 701 557 447 186 147 109 52 43 36 14 1 1 1 EcoRI G'AATT_C Cuts at: 0 1000 2295 Size: 1000 1295 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1494 2295 Size: 1494 801 FspI TGC'GCA Cuts at: 0 202 1510 2295 Size: 202 1308 785 Fragments arranged by size: 1308 785 202 HaeII r_GCGC'y Cuts at: 0 214 258 286 390 1144 1190 1272 1875 Size: 214 44 28 104 754 46 82 603 Cuts at: 1875 2192 2295 Size: 317 103 Fragments arranged by size: 754 603 317 214 104 103 82 46 44 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1290 1322 2228 2260 2295 Size: 1290 32 906 32 35 Fragments arranged by size: 1290 906 35 32 32 KasI G'GCGC_C Cuts at: 0 282 2295 Size: 282 2013 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1469 2295 Size: 1469 826 MscI TGG'CCA Cuts at: 0 1180 1820 2161 2295 Size: 1180 640 341 134 Fragments arranged by size: 1180 640 341 134 MslI CAynn'nnrTG Cuts at: 0 193 2095 2295 Size: 193 1902 200 Fragments arranged by size: 1902 200 193 MspA1I CmG'CkG Cuts at: 0 822 1174 1259 1407 2295 Size: 822 352 85 148 888 Fragments arranged by size: 888 822 352 148 85 NarI GG'CG_CC Cuts at: 0 283 2295 Size: 283 2012 NotI GC'GGCC_GC Cuts at: 0 1651 2295 Size: 1651 644 PasI CC'CwG_GG Cuts at: 0 867 2295 Size: 867 1428 PfoI T'CCnGG_A Cuts at: 0 341 1424 2295 Size: 341 1083 871 Fragments arranged by size: 1083 871 341 PpuMI rG'GwC_Cy Cuts at: 0 810 1053 1797 2295 Size: 810 243 744 498 Fragments arranged by size: 810 744 498 243 PspOMI G'GGCC_C Cuts at: 0 796 862 1610 2062 2258 2295 Size: 796 66 748 452 196 37 Fragments arranged by size: 796 748 452 196 66 37 PspXI vC'TCGA_Gb Cuts at: 0 1972 2050 2295 Size: 1972 78 245 Fragments arranged by size: 1972 245 78 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1573 1605 2295 Size: 1573 32 690 Fragments arranged by size: 1573 690 32 PstI C_TGCA'G Cuts at: 0 821 1700 2003 2295 Size: 821 879 303 292 Fragments arranged by size: 879 821 303 292 PvuII CAG'CTG Cuts at: 0 1407 2295 Size: 1407 888 SanDI GG'GwC_CC Cuts at: 0 1053 2295 Size: 1053 1242 SapI GCTCTTCn'nnn_ Cuts at: 0 688 2131 2295 Size: 688 1443 164 Fragments arranged by size: 1443 688 164 ScaI AGT'ACT Cuts at: 0 394 2295 Size: 394 1901 SexAI A'CCwGG_T Cuts at: 0 1418 2295 Size: 1418 877 SfcI C'TryA_G Cuts at: 0 817 1696 1999 2295 Size: 817 879 303 296 Fragments arranged by size: 879 817 303 296 SfoI GGC'GCC Cuts at: 0 284 2295 Size: 284 2011 SmaI CCC'GGG Cuts at: 0 1608 1665 2231 2295 Size: 1608 57 566 64 Fragments arranged by size: 1608 566 64 57 SmlI C'TyrA_G Cuts at: 0 106 1570 1972 2050 2295 Size: 106 1464 402 78 245 Fragments arranged by size: 1464 402 245 106 78 StuI AGG'CCT Cuts at: 0 1894 2295 Size: 1894 401 StyI C'CwwG_G Cuts at: 0 1919 2295 Size: 1919 376 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 388 906 1141 2295 Size: 388 518 235 1154 Fragments arranged by size: 1154 518 388 235 TatI w'GTAC_w Cuts at: 0 392 605 1775 2295 Size: 392 213 1170 520 Fragments arranged by size: 1170 520 392 213 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 866 898 2295 Size: 866 32 1397 Fragments arranged by size: 1397 866 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 606 1813 2295 Size: 606 1207 482 Fragments arranged by size: 1207 606 482 XhoI C'TCGA_G Cuts at: 0 1972 2050 2295 Size: 1972 78 245 Fragments arranged by size: 1972 245 78 XmaI C'CCGG_G Cuts at: 0 1606 1663 2229 2295 Size: 1606 57 566 66 Fragments arranged by size: 1606 566 66 57 Enzymes that do cut: AarI AccI AcuI AfeI AflIII AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI BglI BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI BtgI BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I NarI NotI PasI PfoI PpuMI PspOMI PspXI PsrI PstI PvuII SanDI SapI ScaI SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XhoI XmaI Enzymes that do not cut: AatII Acc65I AclI AflII AgeI AhdI AleI AloI AscI AseI AsiSI AvrII BaeI BbsI BclI BfrBI BglII BmrI BmtI BplI BsaBI BsaXI BsiWI BsmBI BspHI BsrDI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EcoICRI EcoRV FseI FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PvuI RsrII SacI SacII SalI SbfI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XmnI ZraI