(Linear) (Six-base) MAPSORT of: KIEE3713.seq Check: 7000 from: 1 to: 6075 KIEE3713.orf With 182 enzymes: SgfI * July 3, 2012 18:16 .. AccI GT'mk_AC Cuts at: 0 2420 4759 6075 Size: 2420 2339 1316 Fragments arranged by size: 2420 2339 1316 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 901 1688 2166 3107 3532 3665 4738 5020 Size: 901 787 478 941 425 133 1073 282 Cuts at: 5020 5228 5417 6075 Size: 208 189 658 Fragments arranged by size: 1073 941 901 787 658 478 425 282 208 189 133 AfeI AGC'GCT Cuts at: 0 1524 5973 6075 Size: 1524 4449 102 Fragments arranged by size: 4449 1524 102 AflII C'TTAA_G Cuts at: 0 2373 4552 6075 Size: 2373 2179 1523 Fragments arranged by size: 2373 2179 1523 AflIII A'CryG_T Cuts at: 0 4010 4377 6075 Size: 4010 367 1698 Fragments arranged by size: 4010 1698 367 AgeI A'CCGG_T Cuts at: 0 3891 6075 Size: 3891 2184 AhdI GACnn_n'nnGTC Cuts at: 0 5049 6075 Size: 5049 1026 AleI CACnn'nnGTG Cuts at: 0 5755 6075 Size: 5755 320 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 4406 6075 Size: 4406 1669 AlwNI CAG_nnn'CTG Cuts at: 0 2999 3187 3208 3847 6054 6075 Size: 2999 188 21 639 2207 21 Fragments arranged by size: 2999 2207 639 188 21 21 ApaI G_GGCC'C Cuts at: 0 954 6075 Size: 954 5121 ApoI r'AATT_y Cuts at: 0 91 180 187 360 1279 1906 2056 2209 Size: 91 89 7 173 919 627 150 153 Cuts at: 2209 2456 2589 2974 3177 3235 3578 3637 3726 Size: 247 133 385 203 58 343 59 89 Cuts at: 3726 4317 4472 6075 Size: 591 155 1603 Fragments arranged by size: 1603 919 627 591 385 343 247 203 173 155 153 150 133 91 89 89 59 58 7 AseI AT'TA_AT Cuts at: 0 1193 6075 Size: 1193 4882 AvaI C'yCGr_G Cuts at: 0 5224 6075 Size: 5224 851 AvrII C'CTAG_G Cuts at: 0 1120 6075 Size: 1120 4955 BamHI G'GATC_C Cuts at: 0 1080 2717 5316 6075 Size: 1080 1637 2599 759 Fragments arranged by size: 2599 1637 1080 759 BanI G'GyrC_C Cuts at: 0 4101 6075 Size: 4101 1974 BanII G_rGCy'C Cuts at: 0 816 954 2702 3927 4228 5741 5779 6075 Size: 816 138 1748 1225 301 1513 38 296 Fragments arranged by size: 1748 1513 1225 816 301 296 138 38 BbsI GAAGACnn'nnnn_ Cuts at: 0 638 1058 1816 2306 2567 3167 5234 6075 Size: 638 420 758 490 261 600 2067 841 Fragments arranged by size: 2067 841 758 638 600 490 420 261 BbvCI CC'TCA_GC Cuts at: 0 14 6075 Size: 14 6061 BciVI GTATCCnnnnn_n' Cuts at: 0 3780 3940 4935 6075 Size: 3780 160 995 1140 Fragments arranged by size: 3780 1140 995 160 BglII A'GATC_T Cuts at: 0 4616 6075 Size: 4616 1459 Bme1580I G_kGCm'C Cuts at: 0 954 5927 6075 Size: 954 4973 148 Fragments arranged by size: 4973 954 148 BmgBI CAC'GTC Cuts at: 0 5286 5494 6075 Size: 5286 208 581 Fragments arranged by size: 5286 581 208 BmrI ACTGGGnnnn_n' Cuts at: 0 957 1703 4023 5863 6032 6075 Size: 957 746 2320 1840 169 43 Fragments arranged by size: 2320 1840 957 746 169 43 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 5760 6075 Size: 5760 315 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1850 1916 5471 6075 Size: 1850 66 3555 604 Fragments arranged by size: 3555 1850 604 66 Bpu10I CC'TnA_GC Cuts at: 0 14 6075 Size: 14 6061 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 403 3517 3919 4240 6075 Size: 403 3114 402 321 1835 Fragments arranged by size: 3114 1835 403 402 321 BsaI GGTCTCn'nnnn_ Cuts at: 0 3825 4561 6075 Size: 3825 736 1514 Fragments arranged by size: 3825 1514 736 BsaAI yAC'GTr Cuts at: 0 66 346 3956 4380 6075 Size: 66 280 3610 424 1695 Fragments arranged by size: 3610 1695 424 280 66 BsaBI GATnn'nnATC Cuts at: 0 2176 6075 Size: 2176 3899 BsaHI Gr'CG_yC Cuts at: 0 332 6075 Size: 332 5743 BsaWI w'CCGG_w Cuts at: 0 3891 6075 Size: 3891 2184 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1396 1426 6075 Size: 1396 30 4649 Fragments arranged by size: 4649 1396 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2380 6075 Size: 2380 3695 BseYI C'CCAG_C Cuts at: 0 933 2949 5007 5740 6075 Size: 933 2016 2058 733 335 Fragments arranged by size: 2058 2016 933 733 335 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1418 1511 4379 4402 5200 6075 Size: 1418 93 2868 23 798 875 Fragments arranged by size: 2868 1418 875 798 93 23 BsiHKAI G_wGCw'C Cuts at: 0 816 2557 4310 4496 5549 5915 6075 Size: 816 1741 1753 186 1053 366 160 Fragments arranged by size: 1753 1741 1053 816 366 186 160 BsmI GAATG_Cn' Cuts at: 0 1924 4176 6075 Size: 1924 2252 1899 Fragments arranged by size: 2252 1924 1899 Bsp1286I G_dGCh'C Cuts at: 0 816 954 2557 2702 3927 4228 4310 4496 Size: 816 138 1603 145 1225 301 82 186 Cuts at: 4496 5549 5741 5779 5915 5927 6075 Size: 1053 192 38 136 12 148 Fragments arranged by size: 1603 1225 1053 816 301 192 186 148 145 138 136 82 38 12 BspHI T'CATG_A Cuts at: 0 2807 6075 Size: 2807 3268 BsrDI GCAATG_nn' Cuts at: 0 108 497 2953 6075 Size: 108 389 2456 3122 Fragments arranged by size: 3122 2456 389 108 BsrFI r'CCGG_y Cuts at: 0 3891 6075 Size: 3891 2184 BssSI C'ACGA_G Cuts at: 0 1848 5881 6075 Size: 1848 4033 194 Fragments arranged by size: 4033 1848 194 BstAPI GCAn_nnn'nTGC Cuts at: 0 5857 6075 Size: 5857 218 BstBI TT'CG_AA Cuts at: 0 3239 6075 Size: 3239 2836 BstEII G'GTnAC_C Cuts at: 0 805 4189 6075 Size: 805 3384 1886 Fragments arranged by size: 3384 1886 805 BstXI CCAn_nnnn'nTGG Cuts at: 0 3920 4578 6075 Size: 3920 658 1497 Fragments arranged by size: 3920 1497 658 BstYI r'GATC_y Cuts at: 0 1011 1080 1560 1982 2321 2717 4214 4616 Size: 1011 69 480 422 339 396 1497 402 Cuts at: 4616 5316 6075 Size: 700 759 Fragments arranged by size: 1497 1011 759 700 480 422 402 396 339 69 BstZ17I GTA'TAC Cuts at: 0 4760 6075 Size: 4760 1315 Bsu36I CC'TnA_GG Cuts at: 0 998 1655 6075 Size: 998 657 4420 Fragments arranged by size: 4420 998 657 BtgI C'CryG_G Cuts at: 0 1968 6075 Size: 1968 4107 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 260 542 6075 Size: 260 282 5533 Fragments arranged by size: 5533 282 260 BtsI GCAGTG_nn' Cuts at: 0 2193 2612 3209 4251 5959 6075 Size: 2193 419 597 1042 1708 116 Fragments arranged by size: 2193 1708 1042 597 419 116 DraI TTT'AAA Cuts at: 0 3455 3642 6075 Size: 3455 187 2433 Fragments arranged by size: 3455 2433 187 DraIII CAC_nnn'GTG Cuts at: 0 856 5857 5886 6075 Size: 856 5001 29 189 Fragments arranged by size: 5001 856 189 29 DrdI GACnn_nn'nnGTC Cuts at: 0 6012 6075 Size: 6012 63 EaeI y'GGCC_r Cuts at: 0 271 892 1205 1589 4055 5256 5430 6075 Size: 271 621 313 384 2466 1201 174 645 Fragments arranged by size: 2466 1201 645 621 384 313 271 174 EarI CTCTTCn'nnn_ Cuts at: 0 713 1862 2308 2396 3828 4559 4577 4853 Size: 713 1149 446 88 1432 731 18 276 Cuts at: 4853 4886 6005 6021 6072 6075 Size: 33 1119 16 51 3 Fragments arranged by size: 1432 1149 1119 731 713 446 276 88 51 33 18 16 3 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 49 1252 6075 Size: 49 1203 4823 Fragments arranged by size: 4823 1203 49 EcoICRI GAG'CTC Cuts at: 0 814 6075 Size: 814 5261 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 901 1688 1850 1916 2166 3107 3532 3665 Size: 901 787 162 66 250 941 425 133 Cuts at: 3665 4738 5020 5228 5417 5471 6075 Size: 1073 282 208 189 54 604 Fragments arranged by size: 1073 941 901 787 604 425 282 250 208 189 162 133 66 54 EcoNI CCTnn'n_nnAGG Cuts at: 0 3839 6075 Size: 3839 2236 EcoO109I rG'GnC_Cy Cuts at: 0 951 1109 1716 4575 5765 6075 Size: 951 158 607 2859 1190 310 Fragments arranged by size: 2859 1190 951 607 310 158 EcoRI G'AATT_C Cuts at: 0 2456 2974 3235 6075 Size: 2456 518 261 2840 Fragments arranged by size: 2840 2456 518 261 EcoRV GAT'ATC Cuts at: 0 3981 6075 Size: 3981 2094 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1416 2551 4531 6075 Size: 1416 1135 1980 1544 Fragments arranged by size: 1980 1544 1416 1135 HaeII r_GCGC'y Cuts at: 0 1526 5975 6075 Size: 1526 4449 100 Fragments arranged by size: 4449 1526 100 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1648 1680 1806 1838 2863 2875 2895 2907 Size: 1648 32 126 32 1025 12 20 12 Cuts at: 2907 3325 3357 3640 3672 3965 3997 5035 5067 Size: 418 32 283 32 293 32 1038 32 Cuts at: 5067 5536 5568 6075 Size: 469 32 507 Fragments arranged by size: 1648 1038 1025 507 469 418 293 283 126 32 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 0 1838 6075 Size: 1838 4237 HindIII A'AGCT_T Cuts at: 0 1473 6075 Size: 1473 4602 HpaI GTT'AAC Cuts at: 0 1838 6075 Size: 1838 4237 MfeI C'AATT_G Cuts at: 0 557 564 1579 6075 Size: 557 7 1015 4496 Fragments arranged by size: 4496 1015 557 7 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 3797 6075 Size: 3797 2278 MscI TGG'CCA Cuts at: 0 273 894 1207 1591 4057 5258 5432 6075 Size: 273 621 313 384 2466 1201 174 643 Fragments arranged by size: 2466 1201 643 621 384 313 273 174 MslI CAynn'nnrTG Cuts at: 0 68 323 4009 4169 5755 6075 Size: 68 255 3686 160 1586 320 Fragments arranged by size: 3686 1586 320 255 160 68 MspA1I CmG'CkG Cuts at: 0 2033 2999 3628 5409 6075 Size: 2033 966 629 1781 666 Fragments arranged by size: 2033 1781 966 666 629 NcoI C'CATG_G Cuts at: 0 1968 6075 Size: 1968 4107 NspI r_CATG'y Cuts at: 0 1030 2674 6075 Size: 1030 1644 3401 Fragments arranged by size: 3401 1644 1030 PasI CC'CwG_GG Cuts at: 0 5347 5772 6075 Size: 5347 425 303 Fragments arranged by size: 5347 425 303 PflMI CCAn_nnn'nTGG Cuts at: 0 5386 6075 Size: 5386 689 PfoI T'CCnGG_A Cuts at: 0 1827 5487 6075 Size: 1827 3660 588 Fragments arranged by size: 3660 1827 588 PmlI CAC'GTG Cuts at: 0 66 346 4380 6075 Size: 66 280 4034 1695 Fragments arranged by size: 4034 1695 280 66 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 5617 5649 6075 Size: 5617 32 426 Fragments arranged by size: 5617 426 32 PpuMI rG'GwC_Cy Cuts at: 0 4575 5765 6075 Size: 4575 1190 310 Fragments arranged by size: 4575 1190 310 PspOMI G'GGCC_C Cuts at: 0 950 6075 Size: 950 5125 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2411 2443 4781 4813 6075 Size: 2411 32 2338 32 1262 Fragments arranged by size: 2411 2338 1262 32 32 PstI C_TGCA'G Cuts at: 0 202 3192 3399 4258 4920 5034 6075 Size: 202 2990 207 859 662 114 1041 Fragments arranged by size: 2990 1041 859 662 207 202 114 PvuII CAG'CTG Cuts at: 0 2033 3628 5409 6075 Size: 2033 1595 1781 666 Fragments arranged by size: 2033 1781 1595 666 SacI G_AGCT'C Cuts at: 0 816 6075 Size: 816 5259 SapI GCTCTTCn'nnn_ Cuts at: 0 2396 6072 6075 Size: 2396 3676 3 Fragments arranged by size: 3676 2396 3 ScaI AGT'ACT Cuts at: 0 2044 6075 Size: 2044 4031 SexAI A'CCwGG_T Cuts at: 0 4017 5761 6075 Size: 4017 1744 314 Fragments arranged by size: 4017 1744 314 SfcI C'TryA_G Cuts at: 0 198 2417 2461 2852 3188 3395 4254 4916 Size: 198 2219 44 391 336 207 859 662 Cuts at: 4916 4966 5030 6075 Size: 50 64 1045 Fragments arranged by size: 2219 1045 859 662 391 336 207 198 64 50 44 SmlI C'TyrA_G Cuts at: 0 382 2373 3532 3898 4219 4552 6075 Size: 382 1991 1159 366 321 333 1523 Fragments arranged by size: 1991 1523 1159 382 366 333 321 SnaBI TAC'GTA Cuts at: 0 3956 6075 Size: 3956 2119 SspI AAT'ATT Cuts at: 0 2663 6075 Size: 2663 3412 StuI AGG'CCT Cuts at: 0 1534 6075 Size: 1534 4541 StyI C'CwwG_G Cuts at: 0 1120 1638 1968 3050 3368 4052 5259 5813 Size: 1120 518 330 1082 318 684 1207 554 Cuts at: 5813 6075 Size: 262 Fragments arranged by size: 1207 1120 1082 684 554 518 330 318 262 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 133 2406 4000 5820 5845 6075 Size: 133 2273 1594 1820 25 230 Fragments arranged by size: 2273 1820 1594 230 133 25 TatI w'GTAC_w Cuts at: 0 2042 3001 4031 6075 Size: 2042 959 1030 2044 Fragments arranged by size: 2044 2042 1030 959 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 4935 4967 5205 5237 6075 Size: 4935 32 238 32 838 Fragments arranged by size: 4935 838 238 32 32 Tth111I GACn'n_nGTC Cuts at: 0 5763 6075 Size: 5763 312 XbaI T'CTAG_A Cuts at: 0 1776 3784 5682 6075 Size: 1776 2008 1898 393 Fragments arranged by size: 2008 1898 1776 393 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 4771 6075 Size: 4771 1304 XmnI GAAnn'nnTTC Cuts at: 0 2237 3120 4340 4994 5075 5678 6075 Size: 2237 883 1220 654 81 603 397 Fragments arranged by size: 2237 1220 883 654 603 397 81 Enzymes that do cut: AccI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApoI AseI AvaI AvrII BamHI BanI BanII BbsI BbvCI BciVI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I NcoI NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PsrI PstI PvuII SacI SapI ScaI SexAI SfcI SmlI SnaBI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AloI ApaLI AscI AsiSI BaeI BbeI BcgI BclI BfrBI BglI BlpI BmtI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrGI BssHII ClaI CspCI EagI FseI FspI FspAI KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PshAI PsiI PspXI PvuI RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI SwaI XhoI XmaI ZraI