(Circular) (Six-base) MAPSORT of: pFN21AE4120.seq Check: 7262 from: 1 to: 6214 pFN21AE4120 6214 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3134 3134 Size: 6214 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4024 278 Size: 53 83 186 936 2488 2468 Fragments arranged by size: 2488 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3233 3370 3557 1937 Size: 1296 137 187 4594 Fragments arranged by size: 4594 1296 187 137 Acc65I G'GTAC_C Cuts at: 3535 3535 Size: 6214 AclI AA'CG_TT Cuts at: 1563 4341 4714 1563 Size: 2778 373 3063 Fragments arranged by size: 3063 2778 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2029 2868 4277 5661 5929 1632 Size: 397 839 1409 1384 268 1917 Fragments arranged by size: 1917 1409 1384 839 397 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6017 AflIII A'CryG_T Cuts at: 1203 2184 5113 6090 1203 Size: 981 2929 977 1327 Fragments arranged by size: 2929 1327 981 977 AgeI A'CCGG_T Cuts at: 5045 5045 Size: 6214 AhdI GACnn_n'nnGTC Cuts at: 4943 4943 Size: 6214 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6214 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6214 AlwNI CAG_nnn'CTG Cuts at: 2368 3957 5529 2368 Size: 1589 1572 3053 Fragments arranged by size: 3053 1589 1572 ApaLI G'TGCA_C Cuts at: 4271 5427 4271 Size: 1156 5058 ApoI r'AATT_y Cuts at: 1493 3523 3749 3901 6010 1493 Size: 2030 226 152 2109 1697 Fragments arranged by size: 2109 2030 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2391 4768 160 Size: 872 1359 2377 1606 Fragments arranged by size: 2377 1606 1359 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 AvaI C'yCGr_G Cuts at: 1870 1943 3420 3539 1870 Size: 73 1477 119 4545 Fragments arranged by size: 4545 1477 119 73 BamHI G'GATC_C Cuts at: 3544 3911 3544 Size: 367 5847 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3535 4990 618 Size: 325 217 627 1748 1455 1842 Fragments arranged by size: 1842 1748 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2140 2634 3491 3533 1391 Size: 749 494 857 42 4072 Fragments arranged by size: 4072 857 749 494 42 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2874 928 Size: 971 975 4268 Fragments arranged by size: 4268 975 971 BbvCI CC'TCA_GC Cuts at: 2400 3432 2400 Size: 1032 5182 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4405 4439 1903 Size: 34 2468 34 3678 Fragments arranged by size: 3678 2468 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4106 5316 4106 Size: 1210 5004 BclI T'GATC_A Cuts at: 1548 1548 Size: 6214 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2897 2940 4825 Size: 107 122 71 631 605 1225 43 1885 Cuts at: 4825 136 Size: 1525 Fragments arranged by size: 1885 1525 1225 631 605 122 107 71 43 BglII A'GATC_T Cuts at: 6209 6209 Size: 6214 BlpI GC'TnA_GC Cuts at: 3631 3631 Size: 6214 Bme1580I G_kGCm'C Cuts at: 1790 4275 5431 1790 Size: 2485 1156 2573 Fragments arranged by size: 2573 2485 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6214 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3513 3945 4903 Size: 416 53 83 399 24 2096 432 958 Cuts at: 4903 442 Size: 1753 Fragments arranged by size: 2096 1753 958 432 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6214 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2390 2436 2774 2808 3272 3398 4874 1239 Size: 1151 46 338 34 464 126 1476 2579 Fragments arranged by size: 2579 1476 1151 464 338 126 46 34 Bpu10I CC'TnA_GC Cuts at: 2060 2400 2492 3009 3432 2060 Size: 340 92 517 423 4842 Fragments arranged by size: 4842 517 423 340 92 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4339 5204 5502 4339 Size: 865 298 5051 Fragments arranged by size: 5051 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2440 4877 882 Size: 617 941 2437 2219 Fragments arranged by size: 2437 2219 941 617 BsaAI yAC'GTr Cuts at: 493 1204 4005 493 Size: 711 2801 2702 Fragments arranged by size: 2801 2702 711 BsaBI GATnn'nnATC Cuts at: 6208 6208 Size: 6214 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4021 4403 5038 275 Size: 53 83 186 936 2488 382 635 1451 Fragments arranged by size: 2488 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3105 4647 5045 5319 5466 5877 3105 Size: 1542 398 274 147 411 3442 Fragments arranged by size: 3442 1542 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2880 2910 3380 3410 3451 Size: 30 165 30 1477 30 470 30 41 Cuts at: 3451 3481 1178 Size: 30 3911 Fragments arranged by size: 3911 1477 470 165 41 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2591 2771 1186 Size: 1405 180 4629 Fragments arranged by size: 4629 1405 180 BseYI C'CCAG_C Cuts at: 3017 5417 5913 3017 Size: 2400 496 3318 Fragments arranged by size: 3318 2400 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2826 1163 Size: 573 1090 4551 Fragments arranged by size: 4551 1090 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3664 4425 5453 1540 Size: 275 186 1663 761 1028 2301 Fragments arranged by size: 2301 1663 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4275 4360 5431 4275 Size: 85 1071 5058 Fragments arranged by size: 5058 1071 85 BsmI GAATG_Cn' Cuts at: 2376 3725 3818 2376 Size: 1349 93 4772 Fragments arranged by size: 4772 1349 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2099 3125 2099 Size: 1026 5188 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2140 2634 3491 3533 4275 4360 5431 Size: 399 350 494 857 42 742 85 1071 Cuts at: 5431 1391 Size: 2174 Fragments arranged by size: 2174 1071 857 742 494 399 350 85 42 BspEI T'CCGG_A Cuts at: 5877 5877 Size: 6214 BspHI T'CATG_A Cuts at: 4103 5833 4103 Size: 1730 4484 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3134 3569 844 Size: 2290 435 3489 Fragments arranged by size: 3489 2290 435 BsrBI CCG'CTC Cuts at: 1394 1823 2487 3097 4101 1394 Size: 429 664 610 1004 3507 Fragments arranged by size: 3507 1004 664 610 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3048 4709 4883 65 Size: 1183 1800 1661 174 1396 Fragments arranged by size: 1800 1661 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2523 4858 5045 1700 Size: 110 713 2335 187 2869 Fragments arranged by size: 2869 2335 713 187 110 BsrGI T'GTAC_A Cuts at: 96 2357 96 Size: 2261 3953 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6214 BssSI C'ACGA_G Cuts at: 4274 5286 4274 Size: 1012 5202 BstAPI GCAn_nnn'nTGC Cuts at: 794 3135 794 Size: 2341 3873 BstBI TT'CG_AA Cuts at: 5018 5018 Size: 6214 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6214 BstYI r'GATC_y Cuts at: 1972 2364 3544 3911 4296 4313 5765 5851 5863 Size: 392 1180 367 385 17 1452 86 12 Cuts at: 5863 6209 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1180 392 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3234 3371 3234 Size: 137 6077 Bsu36I CC'TnA_GG Cuts at: 2574 2574 Size: 6214 BtgI C'CryG_G Cuts at: 513 1065 2004 2689 2823 2845 513 Size: 552 939 685 134 22 3882 Fragments arranged by size: 3882 939 685 552 134 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5475 Fragments arranged by size: 5475 683 56 BtsI GCAGTG_nn' Cuts at: 2920 2950 3730 4524 4544 2920 Size: 30 780 794 20 4590 Fragments arranged by size: 4590 794 780 30 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6214 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6179 DraI TTT'AAA Cuts at: 3518 3518 Size: 6214 DraIII CAC_nnn'GTG Cuts at: 2146 2146 Size: 6214 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5221 809 Size: 507 3905 1802 Fragments arranged by size: 3905 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2903 3661 4550 Size: 54 1425 50 275 18 1073 758 889 Cuts at: 4550 6179 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1073 889 758 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3661 1537 Size: 275 1849 4090 Fragments arranged by size: 4090 1849 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3027 3432 3450 4144 1353 Size: 1674 405 18 694 3423 Fragments arranged by size: 3423 1674 694 405 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2290 2699 4801 5175 5321 387 Size: 1064 138 701 409 2102 374 146 1280 Fragments arranged by size: 2102 1280 1064 701 409 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2029 2390 2436 2774 2808 2868 3272 Size: 393 397 361 46 338 34 60 404 Cuts at: 3272 3398 4277 4874 5661 5929 1239 Size: 126 879 597 787 268 1524 Fragments arranged by size: 1524 879 787 597 404 397 393 361 338 268 126 60 46 34 EcoO109I rG'GnC_Cy Cuts at: 3383 3383 Size: 6214 EcoRI G'AATT_C Cuts at: 3523 3901 3523 Size: 378 5836 EcoRV GAT'ATC Cuts at: 2299 2554 2299 Size: 255 5959 FspI TGC'GCA Cuts at: 2081 4720 2081 Size: 2639 3575 HaeII r_GCGC'y Cuts at: 1678 5361 1678 Size: 3683 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 2422 2454 3425 Size: 32 135 32 599 32 219 32 971 Cuts at: 3425 3451 3457 3483 4855 4887 4929 4961 1373 Size: 26 6 26 1372 32 42 32 2626 Fragments arranged by size: 2626 1372 971 599 219 135 42 32 32 32 32 32 32 26 26 6 HincII GTy'rAC Cuts at: 669 1657 1938 2923 3558 3804 669 Size: 988 281 985 635 246 3079 Fragments arranged by size: 3079 988 985 635 281 246 HindIII A'AGCT_T Cuts at: 748 2127 748 Size: 1379 4835 HpaI GTT'AAC Cuts at: 3804 3804 Size: 6214 KpnI G_GTAC'C Cuts at: 3539 3539 Size: 6214 MfeI C'AATT_G Cuts at: 2027 3813 2027 Size: 1786 4428 MluI A'CGCG_T Cuts at: 6090 6090 Size: 6214 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2678 5328 5512 1305 Size: 1373 2650 184 2007 Fragments arranged by size: 2650 2007 1373 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4392 Fragments arranged by size: 4392 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3297 3303 4172 4531 4690 518 Size: 648 2131 6 869 359 159 2042 Fragments arranged by size: 2131 2042 869 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2318 3250 3630 4307 5455 5700 1606 Size: 712 932 380 677 1148 245 2120 Fragments arranged by size: 2120 1148 932 712 677 380 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2823 2845 513 Size: 552 939 819 22 3882 Fragments arranged by size: 3882 939 819 552 22 NdeI CA'TA_TG Cuts at: 387 387 Size: 6214 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6214 NotI GC'GGCC_GC Cuts at: 3661 3661 Size: 6214 NspI r_CATG'y Cuts at: 1132 1229 2086 2188 3181 3220 3572 5117 1132 Size: 97 857 102 993 39 352 1545 2229 Fragments arranged by size: 2229 1545 993 857 352 102 97 39 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6214 PciI A'CATG_T Cuts at: 2184 5113 2184 Size: 2929 3285 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6214 PfoI T'CCnGG_A Cuts at: 2175 2367 2175 Size: 192 6022 PmeI GTTT'AAAC Cuts at: 3518 3518 Size: 6214 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3086 3118 4283 4315 5796 5828 3086 Size: 32 1165 32 1481 32 3472 Fragments arranged by size: 3472 1481 1165 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6214 PsiI TTA'TAA Cuts at: 3784 3784 Size: 6214 PstI C_TGCA'G Cuts at: 830 3566 830 Size: 2736 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6214 PvuII CAG'CTG Cuts at: 2318 3250 2318 Size: 932 5282 RsrII CG'GwC_CG Cuts at: 2686 2686 Size: 6214 SalI G'TCGA_C Cuts at: 1936 3556 1936 Size: 1620 4594 SbfI CC_TGCA'GG Cuts at: 3566 3566 Size: 6214 ScaI AGT'ACT Cuts at: 4462 6174 4462 Size: 1712 4502 SfcI C'TryA_G Cuts at: 826 1045 2853 3562 4697 5378 5569 826 Size: 219 1808 709 1135 681 191 1471 Fragments arranged by size: 1808 1471 1135 709 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6214 SmaI CCC'GGG Cuts at: 1872 3541 1872 Size: 1669 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3420 4318 5219 5481 820 Size: 197 926 1477 898 901 262 1553 Fragments arranged by size: 1553 1477 926 901 898 262 197 SnaBI TAC'GTA Cuts at: 493 4005 493 Size: 3512 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6214 SphI G_CATG'C Cuts at: 1132 2086 3572 1132 Size: 954 1486 3774 Fragments arranged by size: 3774 1486 954 SspI AAT'ATT Cuts at: 5 52 4138 5 Size: 47 4086 2081 Fragments arranged by size: 4086 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6214 StyI C'CwwG_G Cuts at: 513 1065 2004 2823 2845 2892 3653 513 Size: 552 939 819 22 47 761 3074 Fragments arranged by size: 3074 939 819 761 552 47 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4241 4258 4411 4596 5041 325 Size: 1255 2661 17 153 185 445 1498 Fragments arranged by size: 2661 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2204 2357 2731 Size: 274 80 33 51 1443 227 153 374 Cuts at: 2731 4460 6172 96 Size: 1729 1712 138 Fragments arranged by size: 1729 1712 1443 374 274 227 153 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2880 2912 3380 3412 6174 6206 2880 Size: 32 468 32 2762 32 2888 Fragments arranged by size: 2888 2762 468 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6214 XbaI T'CTAG_A Cuts at: 3550 3550 Size: 6214 XhoI C'TCGA_G Cuts at: 1943 3420 1943 Size: 1477 4737 XmaI C'CCGG_G Cuts at: 1870 3539 1870 Size: 1669 4545 XmnI GAAnn'nnTTC Cuts at: 4343 4343 Size: 6214 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4022 276 Size: 53 83 186 936 2488 2468 Fragments arranged by size: 2488 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI