################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BbsI BciVI BclI BfrI BglII BlnI BpuAI BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I ClaI CpoI DraI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tsp45I Tsp509I Tth111I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AfaI 684 GTAC GT^AC AflIII 672 ACATGT A^C[GA][CT]GT AlwNI 1234 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1317 CCTCAGG CC^T[ACGT]AGG AscI 798 GGCGCGCC GG^CGCGCC AviII 713 TGCGCA TGC^GCA BbeI 697 GGCGCC GGCGC^C BbrPI 643 CACGTG CAC^GTG BbvCI 928 GCTGAGG CCTCAGC(-5/-2) BcgI 530 CGACTCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 403 GCTGAGC GC^T[ACGT]AGC Bpu1102I 403 GCTGAGC GC^T[ACGT]AGC BsaAI 643 CACGTG [CT]AC^GT[GA] BsiEI 836 CGGCCG CG[GA][CT]^CG BsmBI 631 GAGACG CGTCTC(1/5) BspLU11I 672 ACATGT A^CATGT BsrBI 1312 CCGCTC CCGCTC(-3/-3) BstAPI 1044 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1162 CCAGAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1317 CCTCAGG CC^T[ACGT]AGG CelII 403 GCTGAGC GC^T[ACGT]AGC DraIII 744 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 172 GACCCAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 959 CCATGG C^C[GA][CT]GG EagI 836 CGGCCG C^GGCCG EclXI 836 CGGCCG C^GGCCG Eco52I 836 CGGCCG C^GGCCG Eco81I 1317 CCTCAGG CC^T[ACGT]AGG FspI 713 TGCGCA TGC^GCA HgaI 633 GACGC GACGC(5/10) KasI 697 GGCGCC G^GCGCC MaeIII 914 GTTAC ^GT[ACGT]AC MseI 1109 TTAA T^TAA NarI 697 GGCGCC GG^CGCC NcoI 959 CCATGG C^CATGG NspI 672 ACATGT [GA]CATG^[CT] PmaCI 643 CACGTG CAC^GTG PmlI 643 CACGTG CAC^GTG PpuMI 932 AGGTCCC [GA]G^G[AT]CC[CT] RsaI 684 GTAC GT^AC SfoI 697 GGCGCC GGC^GCC TaqI 381 TCGA T^CGA Tru9I 1109 TTAA T^TAA TthHB8I 381 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 633 GACGCC G[GA]^CG[CT]C 697 GGCGCC AlwI 390 GATCC GGATC(4/5) 552 GATCC BfaI 149 CTAG C^TAG 1249 CTAG BglI 283 GCCTGGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 829 GCCCGGGCGGC BsaHI 633 GACGCC G[GA]^CG[CT]C 697 GGCGCC DpnII 390 GATC ^GATC 552 GATC EaeI 836 CGGCCG [CT]^GGCC[GA] 896 TGGCCG EarI 193 CTCTTC CTCTTC(1/4) 238 GAAGAG EcoNI 51 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 207 CCTGCAGCAGG HaeII 697 GGCGCC [GA]GCGC^[CT] 848 AGCGCC Hin1I 633 GACGCC G[GA]^CG[CT]C 697 GGCGCC Ksp632I 193 CTCTTC CTCTTC(1/4) 238 GAAGAG MaeI 149 CTAG C^TAG 1249 CTAG MaeII 644 ACGT A^CGT 704 ACGT MboI 390 GATC ^GATC 552 GATC MboII 238 GAAGA GAAGA(8/7) 194 TCTTC MslI 252 CATGATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 954 CACGCCCATG NaeI 803 GCCGGC GCC^GGC 1280 GCCGGC NdeII 390 GATC ^GATC 552 GATC NgoMIV 803 GCCGGC G^CCGGC 1280 GCCGGC Sau3AI 390 GATC ^GATC 552 GATC SmaI 134 CCCGGG CCC^GGG 830 CCCGGG StuI 151 AGGCCT AGG^CCT 446 AGGCCT XcmI 248 CCAACATGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 575 CCAAGTTGCTGCTGG XmaCI 134 CCCGGG C^CCGGG 830 CCCGGG XmaI 134 CCCGGG C^CCGGG 830 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccII AciI AluI ApaI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI PstI PvuII Sau96I ScrFI SfaNI SfcI StyI TseI TspRI