Restriction map for
pFN21AB7310
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI Alw44I ApaLI AscI AseI
AsnI AviII AvrII BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BseAI BsiEI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
Bst1107I BstZ17I ClaI DraI EagI
EclXI Eco52I EcoRI EcoRV EcoT22I
FbaI FspI HincII HindII HindIII
HpaI KspI MamI MfeI MluI
MroI MseI MunI NdeI NheI
NotI NruI NsiI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI SacII SalI
SbfI ScaI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I XbaI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 3052 GGTACC G^GTACC
AcsI 3802 AAATTC [GA]^AATT[CT]
AhdI 2191 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4099 CCTGAGG CC^T[ACGT]AGG
Aor51HI 4070 AGCGCT AGC^GCT
ApoI 3802 AAATTC [GA]^AATT[CT]
Asp700I 612 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3052 GGTACC G^GTACC
AspEI 2191 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 510 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2265 GGCGCC GGCGC^C
BsaWI 680 ACCGGA [AT]^CCGG[AT]
BsiWI 3092 CGTACG C^GTACG
BsrFI 2812 GCCGGC [GA]^CCGG[CT]
BstBI 4063 TTCGAA TT^CGAA
Bsu36I 4099 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2812 GCCGGC [GA]^CCGG[CT]
CpoI 682 CGGACCG CG^G[AT]CCG
Eam1105I 2191 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4070 AGCGCT AGC^GCT
Eco81I 4099 CCTGAGG CC^T[ACGT]AGG
FseI 2811 GGCCGGCC GGCCGG^CC
KasI 2265 GGCGCC G^GCGCC
KpnI 3052 GGTACC GGTAC^C
NaeI 2812 GCCGGC GCC^GGC
NarI 2265 GGCGCC GG^CGCC
NgoMIV 2812 GCCGGC G^CCGGC
NspV 4063 TTCGAA TT^CGAA
PflFI 510 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 682 CGGACCG CG^G[AT]CCG
SapI 4066 GAAGAGC GCTCTTC(1/4)
SfoI 2265 GGCGCC GGC^GCC
SfuI 4063 TTCGAA TT^CGAA
StuI 389 AGGCCT AGG^CCT
TaqI 4064 TCGA T^CGA
Tsp509I 3803 AATT ^AATT
Tth111I 510 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 4064 TCGA T^CGA
XmnI 612 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1102 GTAGAC GT^[AC][GT]AC
3355 GTCTAC
AcyI 2094 GGCGTC G[GA]^CG[CT]C
2265 GGCGCC
BamHI 1554 GGATCC G^GATCC
1779 GGATCC
BbsI 4017 GAAGAC GAAGAC(2/6)
4244 GAAGAC
BlpI 1043 GCTCAGC GC^T[ACGT]AGC
2738 GCTCAGC
Bpu1102I 1043 GCTCAGC GC^T[ACGT]AGC
2738 GCTCAGC
BpuAI 4017 GAAGAC GAAGAC(2/6)
4244 GAAGAC
BsaHI 2094 GGCGTC G[GA]^CG[CT]C
2265 GGCGCC
BsmI 612 GAATGC GAATGC(1/-1)
3557 GCATTC
BspHI 243 TCATGA T^CATGA
620 TCATGA
BsrDI 1356 CATTGC GCAATG(2/0)
2340 CATTGC
BssSI 849 CACGAG CACGAG(-5/-1)
3102 CACGAG
BstEII 1410 GGTGACC G^GT[ACGT]ACC
2013 GGTCACC
BstPI 1410 GGTGACC G^GT[ACGT]ACC
2013 GGTCACC
CelII 1043 GCTCAGC GC^T[ACGT]AGC
2738 GCTCAGC
DrdI 3823 GACTGGCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4036 GACAACAGGGTC
EarI 4066 GAAGAG CTCTTC(1/4)
4176 GAAGAG
EcoNI 528 CCTACCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2445 CCTGGGCAAGG
EcoO65I 1410 GGTGACC G^GT[ACGT]ACC
2013 GGTCACC
Hin1I 2094 GGCGTC G[GA]^CG[CT]C
2265 GGCGCC
Ksp632I 4066 GAAGAG CTCTTC(1/4)
4176 GAAGAG
MaeII 504 ACGT A^CGT
4343 ACGT
RcaI 243 TCATGA T^CATGA
620 TCATGA
SexAI 255 ACCAGGT A^CC[AT]GGT
2436 ACCAGGT
SfiI 1965 GGCCTATGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3495 GGCCCCCAAGGCC
SmlI 1393 CTCAAG C^T[CT][GA]AG
3667 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TfiI TseI Tsp45I TspRI
Van91I XcmI XhoII XmaCI XmaI