(Circular) (Six-base) MAPSORT of: pFN21AB7310.seq Check: 3463 from: 1 to: 9529 pFN21AB7310 9529 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4109 4382 4531 5398 5576 5767 4109 Size: 273 149 867 178 191 7871 Fragments arranged by size: 7871 867 273 191 178 149 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7339 278 Size: 53 83 186 936 5803 2468 Fragments arranged by size: 5803 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3108 5361 6872 1937 Size: 1171 2253 1511 4594 Fragments arranged by size: 4594 2253 1511 1171 Acc65I G'GTAC_C Cuts at: 5057 6850 5057 Size: 1793 7736 AclI AA'CG_TT Cuts at: 1563 7656 8029 1563 Size: 6093 373 3063 Fragments arranged by size: 6093 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2617 4496 5808 6038 7592 8976 9244 1632 Size: 985 1879 1312 230 1554 1384 268 1917 Fragments arranged by size: 1917 1879 1554 1384 1312 985 268 230 AfeI AGC'GCT Cuts at: 6077 6077 Size: 9529 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9332 AflIII A'CryG_T Cuts at: 1203 8428 9405 1203 Size: 7225 977 1327 Fragments arranged by size: 7225 1327 977 AgeI A'CCGG_T Cuts at: 8360 8360 Size: 9529 AhdI GACnn_n'nnGTC Cuts at: 4201 8258 4201 Size: 4057 5472 AleI CACnn'nnGTG Cuts at: 1166 4025 4052 5021 1166 Size: 2859 27 969 5674 Fragments arranged by size: 5674 2859 969 27 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4070 4461 4817 4887 1184 Size: 2886 391 356 70 5826 Fragments arranged by size: 5826 2886 391 356 70 AlwNI CAG_nnn'CTG Cuts at: 3009 3130 3215 3895 4397 4541 4756 4828 4859 Size: 121 85 680 502 144 215 72 31 Cuts at: 4859 5170 5426 5626 5666 5851 6139 7272 8844 Size: 311 256 200 40 185 288 1133 1572 Cuts at: 8844 3009 Size: 3694 Fragments arranged by size: 3694 1572 1133 680 502 311 288 256 215 200 185 144 121 85 72 40 31 ApaI G_GGCC'C Cuts at: 2653 2733 3555 3713 3982 4321 4360 5106 5145 Size: 80 822 158 269 339 39 746 39 Cuts at: 5145 5417 5789 6421 6674 2653 Size: 272 372 632 253 5508 Fragments arranged by size: 5508 822 746 632 372 339 272 269 253 158 80 39 39 ApaLI G'TGCA_C Cuts at: 7586 8742 7586 Size: 1156 8373 ApoI r'AATT_y Cuts at: 1493 5807 6838 7064 7216 9325 1493 Size: 4314 1031 226 152 2109 1697 Fragments arranged by size: 4314 2109 1697 1031 226 152 AseI AT'TA_AT Cuts at: 160 1032 8083 160 Size: 872 7051 1606 Fragments arranged by size: 7051 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9529 AvaI C'yCGr_G Cuts at: 1870 1943 3060 3267 4149 4306 4735 6153 6215 Size: 73 1117 207 882 157 429 1418 62 Cuts at: 6215 6854 1870 Size: 639 4545 Fragments arranged by size: 4545 1418 1117 882 639 429 207 157 73 62 BamHI G'GATC_C Cuts at: 3559 3784 6859 7226 3559 Size: 225 3075 367 5862 Fragments arranged by size: 5862 3075 367 225 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2137 2643 3227 3380 4121 Size: 325 217 627 350 506 584 153 741 Cuts at: 4121 4153 4270 4301 4445 4519 4871 4909 4936 Size: 32 117 31 144 74 352 38 27 Cuts at: 4936 5057 5588 6376 6629 6850 8305 618 Size: 121 531 788 253 221 1455 1842 Fragments arranged by size: 1842 1455 788 741 627 584 531 506 352 350 325 253 221 217 153 144 121 117 74 38 32 31 27 BanII G_rGCy'C Cuts at: 1391 2321 2418 2653 2733 2949 2981 3196 3546 Size: 930 97 235 80 216 32 215 350 Cuts at: 3546 3555 3665 3713 3982 4166 4321 4360 4385 Size: 9 110 48 269 184 155 39 25 Cuts at: 4385 4844 4952 5106 5114 5145 5417 5789 6170 Size: 459 108 154 8 31 272 372 381 Cuts at: 6170 6276 6421 6674 6778 6848 1391 Size: 106 145 253 104 70 4072 Fragments arranged by size: 4072 930 459 381 372 350 272 269 253 235 216 215 184 155 154 145 110 108 106 104 97 80 70 48 39 32 31 25 9 8 BbeI G_GCGC'C Cuts at: 4274 4274 Size: 9529 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 6029 6256 928 Size: 971 4130 227 4201 Fragments arranged by size: 4201 4130 971 227 BbvCI CC'TCA_GC Cuts at: 3631 4095 5317 5763 6597 3631 Size: 464 1222 446 834 6563 Fragments arranged by size: 6563 1222 834 464 446 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7720 7754 1903 Size: 34 5783 34 3678 Fragments arranged by size: 5783 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7421 8631 7421 Size: 1210 8319 BclI T'GATC_A Cuts at: 1548 1548 Size: 9529 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3096 3236 3550 Size: 107 122 71 631 605 1424 140 314 Cuts at: 3550 3977 4355 5507 6485 8140 136 Size: 427 378 1152 978 1655 1525 Fragments arranged by size: 1655 1525 1424 1152 978 631 605 427 378 314 140 122 107 71 BglII A'GATC_T Cuts at: 9524 9524 Size: 9529 BlpI GC'TnA_GC Cuts at: 3049 4744 6946 3049 Size: 1695 2202 5632 Fragments arranged by size: 5632 2202 1695 Bme1580I G_kGCm'C Cuts at: 1790 2533 2653 2733 2880 2918 3555 3713 3948 Size: 743 120 80 147 38 637 158 235 Cuts at: 3948 3982 4059 4142 4259 4304 4321 4360 4450 Size: 34 77 83 117 45 17 39 90 Cuts at: 4450 4613 4876 5043 5106 5145 5232 5417 5789 Size: 163 263 167 63 39 87 185 372 Cuts at: 5789 6421 6674 7590 8746 1790 Size: 632 253 916 1156 2573 Fragments arranged by size: 2573 1156 916 743 637 632 372 263 253 235 185 167 163 158 147 120 117 90 87 83 80 77 63 45 39 39 38 34 17 BmgBI CAC'GTC Cuts at: 1318 6349 1318 Size: 5031 4498 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2192 2512 3178 Size: 416 53 83 399 24 775 320 666 Cuts at: 3178 3252 3668 4152 4699 5226 5633 7260 8218 Size: 74 416 484 547 527 407 1627 958 Cuts at: 8218 442 Size: 1753 Fragments arranged by size: 1753 1627 958 775 666 547 527 484 416 416 407 399 320 83 74 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9529 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2413 2413 Size: 9529 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2319 3180 3851 5648 5873 5883 6241 6612 Size: 1080 861 671 1797 225 10 358 371 Cuts at: 6612 8189 1239 Size: 1577 2579 Fragments arranged by size: 2579 1797 1577 1080 861 671 371 358 225 10 Bpu10I CC'TnA_GC Cuts at: 2286 2975 3540 3631 3664 4095 4384 4946 5317 Size: 689 565 91 33 431 289 562 371 Cuts at: 5317 5763 6597 2286 Size: 446 834 5218 Fragments arranged by size: 5218 834 689 565 562 446 431 371 289 91 33 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3383 5693 7654 8519 8817 3383 Size: 2310 1961 865 298 4095 Fragments arranged by size: 4095 2310 1961 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3802 4152 5708 5786 8192 882 Size: 617 2303 350 1556 78 2406 2219 Fragments arranged by size: 2406 2303 2219 1556 617 350 78 BsaAI yAC'GTr Cuts at: 493 1204 7320 493 Size: 711 6116 2702 Fragments arranged by size: 6116 2702 711 BsaBI GATnn'nnATC Cuts at: 9523 9523 Size: 9529 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4100 4271 7336 7718 Size: 53 83 186 936 2567 171 3065 382 Cuts at: 7718 8353 275 Size: 635 1451 Fragments arranged by size: 3065 2567 1451 936 635 382 186 171 83 53 BsaWI w'CCGG_w Cuts at: 2685 7962 8360 8634 8781 9192 2685 Size: 5277 398 274 147 411 3022 Fragments arranged by size: 5277 3022 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2977 3007 3927 3957 5259 Size: 30 165 30 1574 30 920 30 1302 Cuts at: 5259 5289 5707 5737 6350 6380 1178 Size: 30 418 30 613 30 4327 Fragments arranged by size: 4327 1574 1302 920 613 418 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2402 2405 3332 3353 3595 3619 3888 3932 Size: 1216 3 927 21 242 24 269 44 Cuts at: 3932 4993 5305 5780 5868 6122 6376 6594 1186 Size: 1061 312 475 88 254 254 218 4121 Fragments arranged by size: 4121 1216 1061 927 475 312 269 254 254 242 218 88 44 24 21 3 BseYI C'CCAG_C Cuts at: 2141 2178 3737 3866 3982 4171 4252 4397 4460 Size: 37 1559 129 116 189 81 145 63 Cuts at: 4460 4503 4528 4562 4749 4875 4883 4925 4951 Size: 43 25 34 187 126 8 42 26 Cuts at: 4951 5137 5144 5238 5331 5440 5462 5511 5519 Size: 186 7 94 93 109 22 49 8 Cuts at: 5519 5813 5882 5893 6656 6689 6765 6780 8732 Size: 294 69 11 763 33 76 15 1952 Cuts at: 8732 9228 2141 Size: 496 2442 Fragments arranged by size: 2442 1952 1559 763 496 294 189 187 186 145 129 126 116 109 94 93 81 76 69 63 49 43 42 37 34 33 26 25 22 15 11 8 8 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2183 4557 4629 4742 4883 5045 5183 Size: 573 447 2374 72 113 141 162 138 Cuts at: 5183 5277 5289 5595 1163 Size: 94 12 306 5097 Fragments arranged by size: 5097 2374 573 447 306 162 141 138 113 94 72 12 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6979 7740 8768 1540 Size: 275 186 4978 761 1028 2301 Fragments arranged by size: 4978 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2949 2981 3196 4922 5114 7590 7675 8746 2949 Size: 32 215 1726 192 2476 85 1071 3732 Fragments arranged by size: 3732 2476 1726 1071 215 192 85 32 BsiWI C'GTAC_G Cuts at: 5097 5097 Size: 9529 BsmI GAATG_Cn' Cuts at: 2623 5562 7040 7133 2623 Size: 2939 1478 93 5019 Fragments arranged by size: 5019 2939 1478 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2879 2903 4107 2879 Size: 24 1204 8301 Fragments arranged by size: 8301 1204 24 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2321 2418 2533 2653 2733 2880 2918 Size: 399 531 97 115 120 80 147 38 Cuts at: 2918 2949 2981 3196 3546 3555 3665 3713 3948 Size: 31 32 215 350 9 110 48 235 Cuts at: 3948 3982 4059 4142 4166 4259 4304 4321 4360 Size: 34 77 83 24 93 45 17 39 Cuts at: 4360 4385 4450 4613 4844 4876 4922 4952 5043 Size: 25 65 163 231 32 46 30 91 Cuts at: 5043 5106 5114 5145 5232 5417 5789 6170 6276 Size: 63 8 31 87 185 372 381 106 Cuts at: 6276 6421 6674 6778 6848 7590 7675 8746 1391 Size: 145 253 104 70 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 531 399 381 372 350 253 235 231 215 185 163 147 145 120 115 110 106 104 97 93 91 87 85 83 80 77 70 65 63 48 46 45 39 38 34 32 32 31 31 30 25 24 17 9 8 BspEI T'CCGG_A Cuts at: 9192 9192 Size: 9529 BspHI T'CATG_A Cuts at: 2248 2625 7418 9148 2248 Size: 377 4793 1730 2629 Fragments arranged by size: 4793 2629 1730 377 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2194 2233 4109 4382 4531 5398 5465 5576 Size: 1350 39 1876 273 149 867 67 111 Cuts at: 5576 5767 6884 844 Size: 191 1117 3489 Fragments arranged by size: 3489 1876 1350 1117 867 273 191 149 111 67 39 BsrBI CCG'CTC Cuts at: 1394 1823 2290 2896 3412 5770 7416 1394 Size: 429 467 606 516 2358 1646 3507 Fragments arranged by size: 3507 2358 1646 606 516 467 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3360 4344 8024 8198 65 Size: 1183 2112 984 3680 174 1396 Fragments arranged by size: 3680 2112 1396 1183 984 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4817 8173 8360 1700 Size: 110 3007 3356 187 2869 Fragments arranged by size: 3356 3007 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9529 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9529 BssSI C'ACGA_G Cuts at: 2854 5107 7589 8601 2854 Size: 2253 2482 1012 3782 Fragments arranged by size: 3782 2482 2253 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2243 5047 5170 794 Size: 1449 2804 123 5153 Fragments arranged by size: 5153 2804 1449 123 BstBI TT'CG_AA Cuts at: 6069 8333 6069 Size: 2264 7265 BstEII G'GTnAC_C Cuts at: 1184 3415 4018 1184 Size: 2231 603 6695 Fragments arranged by size: 6695 2231 603 BstXI CCAn_nnnn'nTGG Cuts at: 2860 3689 4933 5718 6202 2860 Size: 829 1244 785 484 6187 Fragments arranged by size: 6187 1244 829 785 484 BstYI r'GATC_y Cuts at: 1972 2481 2700 3559 3784 6534 6859 7226 7611 Size: 509 219 859 225 2750 325 367 385 Cuts at: 7611 7628 9080 9166 9178 9524 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2750 1977 1452 859 509 385 367 346 325 225 219 86 17 12 Bsu36I CC'TnA_GG Cuts at: 6105 6105 Size: 9529 BtgI C'CryG_G Cuts at: 513 1065 2053 2382 4014 5568 5727 5796 6195 Size: 552 988 329 1632 1554 159 69 399 Cuts at: 6195 6445 6576 513 Size: 250 131 3466 Fragments arranged by size: 3466 1632 1554 988 552 399 329 250 159 131 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3838 4045 5539 487 Size: 683 56 2612 207 1494 4477 Fragments arranged by size: 4477 2612 1494 683 207 56 BtsI GCAGTG_nn' Cuts at: 2916 3517 4374 4641 4826 5192 5430 5747 5941 Size: 601 857 267 185 366 238 317 194 Cuts at: 5941 6725 7045 7839 7859 2916 Size: 784 320 794 20 4586 Fragments arranged by size: 4586 857 794 784 601 366 320 317 267 238 194 185 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9529 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2325 2360 2783 2818 3373 3408 529 Size: 35 1761 35 423 35 555 35 6650 Fragments arranged by size: 6650 1761 555 423 35 35 35 35 DraI TTT'AAA Cuts at: 6833 6833 Size: 9529 DraIII CAC_nnn'GTG Cuts at: 2200 2264 4237 2200 Size: 64 1973 7492 Fragments arranged by size: 7492 1973 64 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5834 6047 8536 809 Size: 507 4518 213 2489 1802 Fragments arranged by size: 4518 2489 1802 507 213 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2255 3237 3291 Size: 54 1425 50 275 18 425 982 54 Cuts at: 3291 4087 4782 4819 5370 5799 6141 6976 7865 Size: 796 695 37 551 429 342 835 889 Cuts at: 7865 9494 8 Size: 1629 43 Fragments arranged by size: 1629 1425 982 889 835 796 695 551 429 425 342 275 54 54 50 43 37 18 EagI C'GGCC_G Cuts at: 1537 1812 6976 1537 Size: 275 5164 4090 Fragments arranged by size: 5164 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 6066 6176 7459 1353 Size: 4713 110 1283 3423 Fragments arranged by size: 4713 3423 1283 110 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2361 3114 3583 3940 3948 4675 Size: 1064 138 772 753 469 357 8 727 Cuts at: 4675 8116 8490 8636 387 Size: 3441 374 146 1280 Fragments arranged by size: 3441 1280 1064 772 753 727 469 374 357 146 138 8 EcoICRI GAG'CTC Cuts at: 2947 2979 3194 5112 2947 Size: 32 215 1918 7364 Fragments arranged by size: 7364 1918 215 32 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2319 2617 3180 3851 4496 5648 5808 Size: 393 687 298 563 671 645 1152 160 Cuts at: 5808 5873 5883 6038 6241 6612 7592 8189 8976 Size: 65 10 155 203 371 980 597 787 Cuts at: 8976 9244 1239 Size: 268 1524 Fragments arranged by size: 1524 1152 980 787 687 671 645 597 563 393 371 298 268 203 160 155 65 10 EcoNI CCTnn'n_nnAGG Cuts at: 2537 4454 2537 Size: 1917 7612 EcoO109I rG'GnC_Cy Cuts at: 2022 2324 2650 2729 3041 3200 3451 3551 3552 Size: 302 326 79 312 159 251 100 1 Cuts at: 3552 3710 3842 3978 3979 4317 4318 4356 4357 Size: 158 132 136 1 338 1 38 1 Cuts at: 4357 4726 4768 4990 5141 5270 5344 5413 5530 Size: 369 42 222 151 129 74 69 117 Cuts at: 5530 5574 5785 5786 5848 6049 6220 6418 6670 Size: 44 211 1 62 201 171 198 252 Cuts at: 6670 6671 6749 6803 2022 Size: 1 78 54 4748 Fragments arranged by size: 4748 369 338 326 312 302 252 251 222 211 201 198 171 159 158 151 136 132 129 117 100 79 78 74 69 62 54 44 42 38 1 1 1 1 1 1 EcoRI G'AATT_C Cuts at: 6838 7216 6838 Size: 378 9151 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 6043 6043 Size: 9529 FseI GG_CCGG'CC Cuts at: 4821 4821 Size: 9529 FspI TGC'GCA Cuts at: 8035 8035 Size: 9529 HaeII r_GCGC'y Cuts at: 1678 3122 4274 5469 5781 6079 6415 8676 1678 Size: 1444 1152 1195 312 298 336 2261 2531 Fragments arranged by size: 2531 2261 1444 1195 1152 336 312 298 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2936 2968 3867 3899 4187 Size: 32 135 32 1364 32 899 32 288 Cuts at: 4187 4219 5259 5291 5707 5739 6212 6244 6668 Size: 32 1040 32 416 32 473 32 424 Cuts at: 6668 6700 8170 8202 8244 8276 1373 Size: 32 1470 32 42 32 2626 Fragments arranged by size: 2626 1470 1364 1040 899 473 424 416 288 135 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 6873 7119 669 Size: 988 281 4935 246 3079 Fragments arranged by size: 4935 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9529 HpaI GTT'AAC Cuts at: 7119 7119 Size: 9529 KasI G'GCGC_C Cuts at: 4270 4270 Size: 9529 KpnI G_GTAC'C Cuts at: 5061 6854 5061 Size: 1793 7736 MfeI C'AATT_G Cuts at: 7128 7128 Size: 9529 MluI A'CGCG_T Cuts at: 9405 9405 Size: 9529 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2232 2492 2886 3458 8643 8827 1305 Size: 927 260 394 572 5185 184 2007 Fragments arranged by size: 5185 2007 927 572 394 260 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5372 5801 6143 10 Size: 54 1425 343 3540 429 342 3396 Fragments arranged by size: 3540 3396 1425 429 343 342 54 MslI CAynn'nnrTG Cuts at: 518 1166 2069 2451 4025 4052 4799 5021 5567 Size: 648 903 382 1574 27 747 222 546 Cuts at: 5567 7487 7846 8005 518 Size: 1920 359 159 2042 Fragments arranged by size: 2042 1920 1574 903 747 648 546 382 359 222 159 27 MspA1I CmG'CkG Cuts at: 1606 2240 3078 3256 3707 3870 4171 4556 4566 Size: 634 838 178 451 163 301 385 10 Cuts at: 4566 4753 4900 4929 5033 5148 5242 5515 5903 Size: 187 147 29 104 115 94 273 388 Cuts at: 5903 6261 6635 6815 6945 7622 8770 9015 1606 Size: 358 374 180 130 677 1148 245 2120 Fragments arranged by size: 2120 1148 838 677 634 451 388 385 374 358 301 273 245 187 180 178 163 147 130 115 104 94 29 10 NaeI GCC'GGC Cuts at: 4819 4819 Size: 9529 NarI GG'CG_CC Cuts at: 4271 4271 Size: 9529 NcoI C'CATG_G Cuts at: 513 1065 4014 5796 6445 513 Size: 552 2949 1782 649 3597 Fragments arranged by size: 3597 2949 1782 649 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9529 NgoMIV G'CCGG_C Cuts at: 4817 4817 Size: 9529 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9529 NotI GC'GGCC_GC Cuts at: 6976 6976 Size: 9529 NspI r_CATG'y Cuts at: 1132 1229 2068 2310 2905 4798 6887 8432 1132 Size: 97 839 242 595 1893 2089 1545 2229 Fragments arranged by size: 2229 2089 1893 1545 839 595 242 97 PasI CC'CwG_GG Cuts at: 1348 2725 2930 3548 3731 4351 4426 4450 4541 Size: 1377 205 618 183 620 75 24 91 Cuts at: 4541 5053 5840 1348 Size: 512 787 5037 Fragments arranged by size: 5037 1377 787 620 618 512 205 183 91 75 24 PciI A'CATG_T Cuts at: 8428 8428 Size: 9529 PflMI CCAn_nnn'nTGG Cuts at: 1108 2200 2264 2758 3731 4541 1108 Size: 1092 64 494 973 810 6096 Fragments arranged by size: 6096 1092 973 810 494 64 PfoI T'CCnGG_A Cuts at: 3196 3196 Size: 9529 PmeI GTTT'AAAC Cuts at: 6833 6833 Size: 9529 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7598 7630 9111 9143 7598 Size: 32 1481 32 7984 Fragments arranged by size: 7984 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2324 3041 3200 3451 4768 4990 5270 5574 6049 Size: 717 159 251 1317 222 280 304 475 Cuts at: 6049 6220 6803 2324 Size: 171 583 5050 Fragments arranged by size: 5050 1317 717 583 475 304 280 251 222 171 159 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9529 PsiI TTA'TAA Cuts at: 7099 7099 Size: 9529 PspOMI G'GGCC_C Cuts at: 2649 2729 3551 3709 3978 4317 4356 5102 5141 Size: 80 822 158 269 339 39 746 39 Cuts at: 5141 5413 5785 6417 6670 2649 Size: 272 372 632 253 5508 Fragments arranged by size: 5508 822 746 632 372 339 272 269 253 158 80 39 39 PstI C_TGCA'G Cuts at: 830 3891 4674 5395 6135 6266 6528 6881 830 Size: 3061 783 721 740 131 262 353 3478 Fragments arranged by size: 3478 3061 783 740 721 353 262 131 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9529 PvuII CAG'CTG Cuts at: 2240 4753 4900 5242 5903 6261 6815 2240 Size: 2513 147 342 661 358 554 4954 Fragments arranged by size: 4954 2513 661 554 358 342 147 RsrII CG'GwC_CG Cuts at: 2688 2688 Size: 9529 SacI G_AGCT'C Cuts at: 2949 2981 3196 5114 2949 Size: 32 215 1918 7364 Fragments arranged by size: 7364 1918 215 32 SalI G'TCGA_C Cuts at: 1936 6871 1936 Size: 4935 4594 SapI GCTCTTCn'nnn_ Cuts at: 6066 6066 Size: 9529 SbfI CC_TGCA'GG Cuts at: 6881 6881 Size: 9529 ScaI AGT'ACT Cuts at: 7777 9489 7777 Size: 1712 7817 SexAI A'CCwGG_T Cuts at: 2260 4441 2260 Size: 2181 7348 SfcI C'TryA_G Cuts at: 826 1045 3122 3567 3887 4670 5115 5391 5488 Size: 219 2077 445 320 783 445 276 97 Cuts at: 5488 5904 6131 6262 6524 6877 8012 8693 8884 Size: 416 227 131 262 353 1135 681 191 Cuts at: 8884 826 Size: 1471 Fragments arranged by size: 2077 1471 1135 783 681 445 445 416 353 320 276 262 227 219 191 131 97 SfiI GGCCn_nnn'nGGCC Cuts at: 3977 5507 3977 Size: 1530 7999 SfoI GGC'GCC Cuts at: 4272 4272 Size: 9529 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9529 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9529 SmaI CCC'GGG Cuts at: 1872 4308 4737 6155 6856 1872 Size: 2436 429 1418 701 4545 Fragments arranged by size: 4545 2436 1418 701 429 SmlI C'TyrA_G Cuts at: 820 1017 1943 3398 5672 7633 8534 8796 820 Size: 197 926 1455 2274 1961 901 262 1553 Fragments arranged by size: 2274 1961 1553 1455 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 7320 493 Size: 6827 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9529 SphI G_CATG'C Cuts at: 1132 6887 1132 Size: 5755 3774 SspI AAT'ATT Cuts at: 5 52 7453 5 Size: 47 7401 2081 Fragments arranged by size: 7401 2081 47 StuI AGG'CCT Cuts at: 1517 2396 1517 Size: 879 8650 StyI C'CwwG_G Cuts at: 513 1065 2111 2538 2733 2988 3231 3759 4014 Size: 552 1046 427 195 255 243 528 255 Cuts at: 4014 4548 5409 5505 5781 5796 6445 6513 6968 Size: 534 861 96 276 15 649 68 455 Cuts at: 6968 513 Size: 3074 Fragments arranged by size: 3074 1046 861 649 552 534 528 455 427 276 255 255 243 195 96 68 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2155 2211 2811 2838 3434 3812 4163 Size: 1255 575 56 600 27 596 378 351 Cuts at: 4163 4487 4591 4663 4939 5323 6703 7556 7573 Size: 324 104 72 276 384 1380 853 17 Cuts at: 7573 7726 7911 8356 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1380 1255 853 600 596 575 445 384 378 351 324 276 185 153 104 72 56 27 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4482 7775 9487 Size: 274 80 33 51 1443 2505 3293 1712 Cuts at: 9487 96 Size: 138 Fragments arranged by size: 3293 2505 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2260 2292 2977 3009 3483 3515 3927 3959 9489 Size: 32 685 32 474 32 412 32 5530 Cuts at: 9489 9521 2260 Size: 32 2268 Fragments arranged by size: 5530 2268 685 474 412 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2518 1316 Size: 1202 8327 XbaI T'CTAG_A Cuts at: 6865 6865 Size: 9529 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2197 2805 4132 4358 4757 5566 2197 Size: 608 1327 226 399 809 6160 Fragments arranged by size: 6160 1327 809 608 399 226 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9529 XmaI C'CCGG_G Cuts at: 1870 4306 4735 6153 6854 1870 Size: 2436 429 1418 701 4545 Fragments arranged by size: 4545 2436 1418 701 429 XmnI GAAnn'nnTTC Cuts at: 2621 7658 2621 Size: 5037 4492 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7337 276 Size: 53 83 186 936 5803 2468 Fragments arranged by size: 5803 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BstZ17I EcoRV FspAI NruI NsiI PacI PmlI PspXI PsrI SacII SanDI SrfI SwaI