(Circular) (Six-base) MAPSORT of: pFN21AB9670.seq Check: 6951 from: 1 to: 5524 pFN21AB9670 5524 bp With 182 enzymes: SgfI * March 28, 2012 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3334 278 Size: 53 83 186 936 1798 2468 Fragments arranged by size: 2468 1798 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2274 2867 1937 Size: 337 593 4594 Fragments arranged by size: 4594 593 337 Acc65I G'GTAC_C Cuts at: 2845 2845 Size: 5524 AclI AA'CG_TT Cuts at: 1563 3651 4024 1563 Size: 2088 373 3063 Fragments arranged by size: 3063 2088 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2413 3587 4971 5239 1632 Size: 781 1174 1384 268 1917 Fragments arranged by size: 1917 1384 1174 781 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5327 AflIII A'CryG_T Cuts at: 1203 4423 5400 1203 Size: 3220 977 1327 Fragments arranged by size: 3220 1327 977 AgeI A'CCGG_T Cuts at: 4355 4355 Size: 5524 AhdI GACnn_n'nnGTC Cuts at: 4253 4253 Size: 5524 AleI CACnn'nnGTG Cuts at: 1166 2222 1166 Size: 1056 4468 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5524 AlwNI CAG_nnn'CTG Cuts at: 3267 4839 3267 Size: 1572 3952 ApaLI G'TGCA_C Cuts at: 3581 4737 3581 Size: 1156 4368 ApoI r'AATT_y Cuts at: 1493 2833 3059 3211 5320 1493 Size: 1340 226 152 2109 1697 Fragments arranged by size: 2109 1697 1340 226 152 AseI AT'TA_AT Cuts at: 160 1032 4078 160 Size: 872 3046 1606 Fragments arranged by size: 3046 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5524 AvaI C'yCGr_G Cuts at: 1870 1943 2849 1870 Size: 73 906 4545 Fragments arranged by size: 4545 906 73 BamHI G'GATC_C Cuts at: 2854 3221 2854 Size: 367 5157 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2845 4300 618 Size: 325 217 627 1058 1455 1842 Fragments arranged by size: 1842 1455 1058 627 325 217 BanII G_rGCy'C Cuts at: 1391 2012 2843 1391 Size: 621 831 4072 Fragments arranged by size: 4072 831 621 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2150 2335 2437 2597 928 Size: 971 251 185 102 160 3855 Fragments arranged by size: 3855 971 251 185 160 102 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3715 3749 1903 Size: 34 1778 34 3678 Fragments arranged by size: 3678 1778 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3416 4626 3416 Size: 1210 4314 BclI T'GATC_A Cuts at: 1548 1548 Size: 5524 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4135 136 Size: 107 122 71 631 605 2463 1525 Fragments arranged by size: 2463 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5519 5519 Size: 5524 BlpI GC'TnA_GC Cuts at: 2941 2941 Size: 5524 Bme1580I G_kGCm'C Cuts at: 1790 3585 4741 1790 Size: 1795 1156 2573 Fragments arranged by size: 2573 1795 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5524 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2229 3255 4213 Size: 416 53 83 399 24 812 1026 958 Cuts at: 4213 442 Size: 1753 Fragments arranged by size: 1753 1026 958 812 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5524 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2089 2105 2498 2089 Size: 16 393 5115 Fragments arranged by size: 5115 393 16 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2409 2493 2577 2745 2829 4184 1239 Size: 1170 84 84 168 84 1355 2579 Fragments arranged by size: 2579 1355 1170 168 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3649 4514 4812 3649 Size: 865 298 4361 Fragments arranged by size: 4361 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2077 4187 882 Size: 617 578 2110 2219 Fragments arranged by size: 2219 2110 617 578 BsaAI yAC'GTr Cuts at: 493 1204 3315 493 Size: 711 2111 2702 Fragments arranged by size: 2702 2111 711 BsaBI GATnn'nnATC Cuts at: 5518 5518 Size: 5524 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3331 3713 4348 275 Size: 53 83 186 936 1798 382 635 1451 Fragments arranged by size: 1798 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3957 4355 4629 4776 5187 3957 Size: 398 274 147 411 4294 Fragments arranged by size: 4294 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5299 Fragments arranged by size: 5299 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5524 BseYI C'CCAG_C Cuts at: 4727 5223 4727 Size: 496 5028 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2030 2428 2596 1163 Size: 573 294 398 168 4091 Fragments arranged by size: 4091 573 398 294 168 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2974 3735 4763 1540 Size: 275 186 973 761 1028 2301 Fragments arranged by size: 2301 1028 973 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3585 3670 4741 3585 Size: 85 1071 4368 Fragments arranged by size: 4368 1071 85 BsmI GAATG_Cn' Cuts at: 2243 3035 3128 2243 Size: 792 93 4639 Fragments arranged by size: 4639 792 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2843 3585 3670 4741 1391 Size: 399 222 831 742 85 1071 2174 Fragments arranged by size: 2174 1071 831 742 399 222 85 BspEI T'CCGG_A Cuts at: 5187 5187 Size: 5524 BspHI T'CATG_A Cuts at: 3413 5143 3413 Size: 1730 3794 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2059 2343 2879 844 Size: 1215 284 536 3489 Fragments arranged by size: 3489 1215 536 284 BsrBI CCG'CTC Cuts at: 1394 1823 3411 1394 Size: 429 1588 3507 Fragments arranged by size: 3507 1588 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4019 4193 65 Size: 1183 2771 174 1396 Fragments arranged by size: 2771 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4168 4355 1700 Size: 110 2358 187 2869 Fragments arranged by size: 2869 2358 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5524 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5524 BssSI C'ACGA_G Cuts at: 3584 4596 3584 Size: 1012 4512 BstAPI GCAn_nnn'nTGC Cuts at: 794 2583 794 Size: 1789 3735 BstBI TT'CG_AA Cuts at: 4328 4328 Size: 5524 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5524 BstXI CCAn_nnnn'nTGG Cuts at: 2249 2249 Size: 5524 BstYI r'GATC_y Cuts at: 1972 2799 2854 3221 3606 3623 5075 5161 5173 Size: 827 55 367 385 17 1452 86 12 Cuts at: 5173 5519 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 827 385 367 346 86 55 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4972 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2034 487 Size: 683 56 808 3977 Fragments arranged by size: 3977 808 683 56 BtsI GCAGTG_nn' Cuts at: 2584 2637 3040 3834 3854 2584 Size: 53 403 794 20 4254 Fragments arranged by size: 4254 794 403 53 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5524 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2146 2181 2482 2517 529 Size: 35 1582 35 301 35 3536 Fragments arranged by size: 3536 1582 301 35 35 35 DraI TTT'AAA Cuts at: 2828 2828 Size: 5524 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4531 809 Size: 507 3215 1802 Fragments arranged by size: 3215 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2971 3860 5489 Size: 54 1425 50 275 18 1141 889 1629 Cuts at: 5489 8 Size: 43 Fragments arranged by size: 1629 1425 1141 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2971 1537 Size: 275 1159 4090 Fragments arranged by size: 4090 1159 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2530 3454 1353 Size: 1177 924 3423 Fragments arranged by size: 3423 1177 924 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4111 4485 4631 387 Size: 1064 138 2522 374 146 1280 Fragments arranged by size: 2522 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2409 2413 2493 2577 2745 2829 3587 Size: 393 777 4 80 84 168 84 758 Cuts at: 3587 4184 4971 5239 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 777 758 597 393 268 168 84 84 80 4 EcoRI G'AATT_C Cuts at: 2833 3211 2833 Size: 378 5146 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2293 2293 Size: 5524 FspI TGC'GCA Cuts at: 4030 4030 Size: 5524 HaeII r_GCGC'y Cuts at: 1678 4671 1678 Size: 2993 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4165 4197 4239 4271 1373 Size: 32 135 32 2593 32 42 32 2626 Fragments arranged by size: 2626 2593 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2275 2868 3114 669 Size: 988 281 337 593 246 3079 Fragments arranged by size: 3079 988 593 337 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5524 HpaI GTT'AAC Cuts at: 3114 3114 Size: 5524 KpnI G_GTAC'C Cuts at: 2849 2849 Size: 5524 MfeI C'AATT_G Cuts at: 3123 3123 Size: 5524 MluI A'CGCG_T Cuts at: 5400 5400 Size: 5524 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2644 4638 4822 1305 Size: 1339 1994 184 2007 Fragments arranged by size: 2007 1994 1339 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3702 Fragments arranged by size: 3702 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2097 2222 3482 3841 4000 518 Size: 648 931 125 1260 359 159 2042 Fragments arranged by size: 2042 1260 931 648 359 159 125 MspA1I CmG'CkG Cuts at: 1606 2940 3617 4765 5010 1606 Size: 1334 677 1148 245 2120 Fragments arranged by size: 2120 1334 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4972 NdeI CA'TA_TG Cuts at: 387 387 Size: 5524 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5524 NotI GC'GGCC_GC Cuts at: 2971 2971 Size: 5524 NspI r_CATG'y Cuts at: 1132 1229 2882 4427 1132 Size: 97 1653 1545 2229 Fragments arranged by size: 2229 1653 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5524 PciI A'CATG_T Cuts at: 4423 4423 Size: 5524 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5524 PmeI GTTT'AAAC Cuts at: 2828 2828 Size: 5524 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3593 3625 5106 5138 3593 Size: 32 1481 32 3979 Fragments arranged by size: 3979 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5524 PsiI TTA'TAA Cuts at: 3094 3094 Size: 5524 PstI C_TGCA'G Cuts at: 830 2644 2876 830 Size: 1814 232 3478 Fragments arranged by size: 3478 1814 232 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5524 SalI G'TCGA_C Cuts at: 1936 2273 2866 1936 Size: 337 593 4594 Fragments arranged by size: 4594 593 337 SbfI CC_TGCA'GG Cuts at: 2876 2876 Size: 5524 ScaI AGT'ACT Cuts at: 3772 5484 3772 Size: 1712 3812 SfcI C'TryA_G Cuts at: 826 1045 2640 2686 2872 4007 4688 4879 826 Size: 219 1595 46 186 1135 681 191 1471 Fragments arranged by size: 1595 1471 1135 681 219 191 186 46 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5524 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5524 SmaI CCC'GGG Cuts at: 1872 2851 1872 Size: 979 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3628 4529 4791 820 Size: 197 926 1685 901 262 1553 Fragments arranged by size: 1685 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3315 493 Size: 2822 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5524 SphI G_CATG'C Cuts at: 1132 2882 1132 Size: 1750 3774 SspI AAT'ATT Cuts at: 5 52 3448 5 Size: 47 3396 2081 Fragments arranged by size: 3396 2081 47 StuI AGG'CCT Cuts at: 1517 2762 1517 Size: 1245 4279 StyI C'CwwG_G Cuts at: 513 1065 2963 513 Size: 552 1898 3074 Fragments arranged by size: 3074 1898 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2478 3551 3568 3721 3906 4351 325 Size: 1255 898 1073 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1073 898 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3770 5482 96 Size: 274 80 33 51 1443 1793 1712 138 Fragments arranged by size: 1793 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5484 5516 5484 Size: 32 5492 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5524 XbaI T'CTAG_A Cuts at: 2860 2860 Size: 5524 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2384 2384 Size: 5524 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5524 XmaI C'CCGG_G Cuts at: 1870 2849 1870 Size: 979 4545 XmnI GAAnn'nnTTC Cuts at: 2342 2594 3653 2342 Size: 252 1059 4213 Fragments arranged by size: 4213 1059 252 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3332 276 Size: 53 83 186 936 1798 2468 Fragments arranged by size: 2468 1798 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI