Restriction map for
pFN21AB9659
(Circular) (Six-base) MAPSORT of: pFN21AB9659.seq Check: 3690 from: 1 to: 6169
pFN21AB9659 6169 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3979 278
Size: 53 83 186 936 2443 2468
Fragments arranged by size:
2468 2443 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3512 1937
Size: 1575 4594
Acc65I G'GTAC_C
Cuts at: 3490 3490
Size: 6169
AclI AA'CG_TT
Cuts at: 1563 4296 4669 1563
Size: 2733 373 3063
Fragments arranged by size:
3063 2733 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2151 4232 5616 5884 1632
Size: 519 2081 1384 268 1917
Fragments arranged by size:
2081 1917 1384 519 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5972
AflIII A'CryG_T
Cuts at: 1203 5068 6045 1203
Size: 3865 977 1327
Fragments arranged by size:
3865 1327 977
AgeI A'CCGG_T
Cuts at: 2762 5000 2762
Size: 2238 3931
AhdI GACnn_n'nnGTC
Cuts at: 4898 4898
Size: 6169
AleI CACnn'nnGTG
Cuts at: 1166 2511 2595 2931 3267 1166
Size: 1345 84 336 336 4068
Fragments arranged by size:
4068 1345 336 336 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6169
AlwNI CAG_nnn'CTG
Cuts at: 3912 5484 3912
Size: 1572 4597
ApaLI G'TGCA_C
Cuts at: 4226 5382 4226
Size: 1156 5013
ApoI r'AATT_y
Cuts at: 1493 2166 2250 2334 2418 2586 2754 2838 2922
Size: 673 84 84 84 168 168 84 84
Cuts at: 2922 3090 3342 3478 3704 3856 5965 1493
Size: 168 252 136 226 152 2109 1697
Fragments arranged by size:
2109 1697 673 252 226 168 168 168
152 136 84 84 84 84 84
AseI AT'TA_AT
Cuts at: 160 1032 4723 160
Size: 872 3691 1606
Fragments arranged by size:
3691 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
AvaI C'yCGr_G
Cuts at: 1870 1943 3494 1870
Size: 73 1551 4545
Fragments arranged by size:
4545 1551 73
BamHI G'GATC_C
Cuts at: 3499 3866 3499
Size: 367 5802
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3249 3490 4945 618
Size: 325 217 627 1462 241 1455 1842
Fragments arranged by size:
1842 1462 1455 627 325 241 217
BanII G_rGCy'C
Cuts at: 1391 3488 1391
Size: 2097 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2139 3056 928
Size: 971 240 917 4041
Fragments arranged by size:
4041 971 917 240
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4360 4394 1903
Size: 34 2423 34 3678
Fragments arranged by size:
3678 2423 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2162 4061 5271 2162
Size: 1899 1210 3060
Fragments arranged by size:
3060 1899 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6169
BfrBI ATG'CAT
Cuts at: 3010 3010
Size: 6169
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4780 136
Size: 107 122 71 631 605 3108 1525
Fragments arranged by size:
3108 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2902 6164 2902
Size: 3262 2907
BlpI GC'TnA_GC
Cuts at: 3586 3586
Size: 6169
Bme1580I G_kGCm'C
Cuts at: 1790 4230 5386 1790
Size: 2440 1156 2573
Fragments arranged by size:
2573 2440 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6169
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3900 4858 442
Size: 416 53 83 399 24 2483 958 1753
Fragments arranged by size:
2483 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3036 4829 1239
Size: 1797 1793 2579
Fragments arranged by size:
2579 1797 1793
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3304 4294 5159 5457 3304
Size: 990 865 298 4016
Fragments arranged by size:
4016 990 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4832 882
Size: 617 3333 2219
Fragments arranged by size:
3333 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3960 493
Size: 711 2756 2702
Fragments arranged by size:
2756 2702 711
BsaBI GATnn'nnATC
Cuts at: 6163 6163
Size: 6169
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3976 4358 4993 275
Size: 53 83 186 936 2443 382 635 1451
Fragments arranged by size:
2443 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2762 4602 5000 5274 5421 5832 2762
Size: 1840 398 274 147 411 3099
Fragments arranged by size:
3099 1840 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5944
Fragments arranged by size:
5944 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6169
BseYI C'CCAG_C
Cuts at: 5372 5868 5372
Size: 496 5673
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5596
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3619 4380 5408 1540
Size: 275 186 1618 761 1028 2301
Fragments arranged by size:
2301 1618 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4230 4315 5386 4230
Size: 85 1071 5013
Fragments arranged by size:
5013 1071 85
BsmI GAATG_Cn'
Cuts at: 2282 2702 3012 3680 3773 2282
Size: 420 310 668 93 4678
Fragments arranged by size:
4678 668 420 310 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3488 4230 4315 5386 1391
Size: 399 1698 742 85 1071 2174
Fragments arranged by size:
2174 1698 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5832 5832
Size: 6169
BspHI T'CATG_A
Cuts at: 4058 5788 4058
Size: 1730 4439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3524 844
Size: 2680 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4056 1394
Size: 429 2233 3507
Fragments arranged by size:
3507 2233 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4664 4838 65
Size: 1183 3416 174 1396
Fragments arranged by size:
3416 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2762 4813 5000 1700
Size: 110 952 2051 187 2869
Fragments arranged by size:
2869 2051 952 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6169
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6169
BssSI C'ACGA_G
Cuts at: 4229 5241 4229
Size: 1012 5157
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2318 794
Size: 1524 4645
BstBI TT'CG_AA
Cuts at: 4973 4973
Size: 6169
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6169
BstYI r'GATC_y
Cuts at: 1972 2090 2902 3499 3866 4251 4268 5720 5806
Size: 118 812 597 367 385 17 1452 86
Cuts at: 5806 5818 6164 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 812 597 385 367 346 118
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4678
Fragments arranged by size:
4678 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5430
Fragments arranged by size:
5430 683 56
BtsI GCAGTG_nn'
Cuts at: 2647 2731 3685 4479 4499 2647
Size: 84 954 794 20 4317
Fragments arranged by size:
4317 954 794 84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6169
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2269 2304 2689 2724 529
Size: 35 1705 35 385 35 3974
Fragments arranged by size:
3974 1705 385 35 35 35
DraI TTT'AAA
Cuts at: 3473 3473
Size: 6169
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5176 809
Size: 507 3860 1802
Fragments arranged by size:
3860 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3616 4505 6134
Size: 54 1425 50 275 18 1786 889 1629
Cuts at: 6134 8
Size: 43
Fragments arranged by size:
1786 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3616 1537
Size: 275 1804 4090
Fragments arranged by size:
4090 1804 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4099 1353
Size: 2746 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2683 4756 5130 5276 387
Size: 1064 138 1094 2073 374 146 1280
Fragments arranged by size:
2073 1280 1094 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2151 3036 4232 4829 5616 5884 1239
Size: 393 519 885 1196 597 787 268 1524
Fragments arranged by size:
1524 1196 885 787 597 519 393 268
EcoRI G'AATT_C
Cuts at: 2166 2250 2334 2418 2586 2754 2838 2922 3090
Size: 84 84 84 168 168 84 84 168
Cuts at: 3090 3342 3478 3856 2166
Size: 252 136 378 4479
Fragments arranged by size:
4479 378 252 168 168 168 136 84
84 84 84 84
FspI TGC'GCA
Cuts at: 4675 4675
Size: 6169
HaeII r_GCGC'y
Cuts at: 1678 5316 1678
Size: 3638 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2110 2142 2484 2516 4810
Size: 32 135 32 538 32 342 32 2294
Cuts at: 4810 4842 4884 4916 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2294 538 342 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3513 3759 669
Size: 988 281 1575 246 3079
Fragments arranged by size:
3079 1575 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6169
HpaI GTT'AAC
Cuts at: 3759 3759
Size: 6169
KpnI G_GTAC'C
Cuts at: 3494 3494
Size: 6169
MfeI C'AATT_G
Cuts at: 3768 3768
Size: 6169
MluI A'CGCG_T
Cuts at: 6045 6045
Size: 6169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3187 5283 5467 1305
Size: 1882 2096 184 2007
Fragments arranged by size:
2096 2007 1882 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4347
Fragments arranged by size:
4347 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2109 2175 2259 2343 2427 2511 2595
Size: 648 943 66 84 84 84 84 84
Cuts at: 2595 2679 2763 2779 2847 2931 3099 3183 3267
Size: 84 84 16 68 84 168 84 84
Cuts at: 3267 3351 4127 4486 4645 518
Size: 84 776 359 159 2042
Fragments arranged by size:
2042 943 776 648 359 168 159 84
84 84 84 84 84 84 84 84
84 84 68 66 16
MspA1I CmG'CkG
Cuts at: 1606 3585 4262 5410 5655 1606
Size: 1979 677 1148 245 2120
Fragments arranged by size:
2120 1979 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4678
Fragments arranged by size:
4678 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6169
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6169
NotI GC'GGCC_GC
Cuts at: 3616 3616
Size: 6169
NsiI A_TGCA'T
Cuts at: 3012 3012
Size: 6169
NspI r_CATG'y
Cuts at: 1132 1229 2140 2709 3527 5072 1132
Size: 97 911 569 818 1545 2229
Fragments arranged by size:
2229 1545 911 818 569 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6169
PciI A'CATG_T
Cuts at: 5068 5068
Size: 6169
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6169
PmeI GTTT'AAAC
Cuts at: 3473 3473
Size: 6169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4238 4270 5751 5783 4238
Size: 32 1481 32 4624
Fragments arranged by size:
4624 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6169
PsiI TTA'TAA
Cuts at: 3739 3739
Size: 6169
PstI C_TGCA'G
Cuts at: 830 3521 830
Size: 2691 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6169
SalI G'TCGA_C
Cuts at: 1936 3511 1936
Size: 1575 4594
SbfI CC_TGCA'GG
Cuts at: 3521 3521
Size: 6169
ScaI AGT'ACT
Cuts at: 4417 6129 4417
Size: 1712 4457
SfcI C'TryA_G
Cuts at: 826 1045 3517 4652 5333 5524 826
Size: 219 2472 1135 681 191 1471
Fragments arranged by size:
2472 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6169
SmaI CCC'GGG
Cuts at: 1872 3496 1872
Size: 1624 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3319 4273 5174 5436 820
Size: 197 926 1376 954 901 262 1553
Fragments arranged by size:
1553 1376 954 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3960 493
Size: 3467 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6169
SphI G_CATG'C
Cuts at: 1132 3527 1132
Size: 2395 3774
SspI AAT'ATT
Cuts at: 5 52 4093 5
Size: 47 4041 2081
Fragments arranged by size:
4041 2081 47
StuI AGG'CCT
Cuts at: 1517 2297 2381 2484 2801 2885 3137 1517
Size: 780 84 103 317 84 252 4549
Fragments arranged by size:
4549 780 317 252 103 84 84
StyI C'CwwG_G
Cuts at: 513 1065 2004 3608 513
Size: 552 939 1604 3074
Fragments arranged by size:
3074 1604 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4196 4213 4366 4551 4996 325
Size: 1255 2616 17 153 185 445 1498
Fragments arranged by size:
2616 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4415 6127 96
Size: 274 80 33 51 1443 2438 1712 138
Fragments arranged by size:
2438 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6129 6161 6129
Size: 32 6137
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6169
XbaI T'CTAG_A
Cuts at: 3505 3505
Size: 6169
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6169
XmaI C'CCGG_G
Cuts at: 1870 3494 1870
Size: 1624 4545
XmnI GAAnn'nnTTC
Cuts at: 2980 4298 2980
Size: 1318 4851
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3977 276
Size: 53 83 186 936 2443 2468
Fragments arranged by size:
2468 2443 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI