(Circular) (Six-base) MAPSORT of: pFN21AB9643.seq Check: 4040 from: 1 to: 5926 pFN21AB9643 5926 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3736 278 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3269 1937 Size: 1332 4594 Acc65I G'GTAC_C Cuts at: 3247 3247 Size: 5926 AclI AA'CG_TT Cuts at: 1563 4053 4426 1563 Size: 2490 373 3063 Fragments arranged by size: 3063 2490 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2172 2249 3049 3989 5373 5641 1632 Size: 540 77 800 940 1384 268 1917 Fragments arranged by size: 1917 1384 940 800 540 268 77 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5729 AflIII A'CryG_T Cuts at: 1203 2380 4825 5802 1203 Size: 1177 2445 977 1327 Fragments arranged by size: 2445 1327 1177 977 AgeI A'CCGG_T Cuts at: 4757 4757 Size: 5926 AhdI GACnn_n'nnGTC Cuts at: 2460 4655 2460 Size: 2195 3731 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5926 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5926 AlwNI CAG_nnn'CTG Cuts at: 2195 2527 3669 5241 2195 Size: 332 1142 1572 2880 Fragments arranged by size: 2880 1572 1142 332 ApaLI G'TGCA_C Cuts at: 3983 5139 3983 Size: 1156 4770 ApoI r'AATT_y Cuts at: 1493 2134 2555 2601 3166 3200 3235 3461 3613 Size: 641 421 46 565 34 35 226 152 Cuts at: 3613 5722 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 641 565 421 226 152 46 35 34 AseI AT'TA_AT Cuts at: 160 1032 2202 4480 160 Size: 872 1170 2278 1606 Fragments arranged by size: 2278 1606 1170 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 AvaI C'yCGr_G Cuts at: 1870 1943 3251 1870 Size: 73 1308 4545 Fragments arranged by size: 4545 1308 73 BamHI G'GATC_C Cuts at: 3256 3623 3256 Size: 367 5559 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3247 4702 618 Size: 325 217 627 1460 1455 1842 Fragments arranged by size: 1842 1460 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3245 1391 Size: 1854 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2377 2871 928 Size: 971 478 494 3983 Fragments arranged by size: 3983 971 494 478 BbvCI CC'TCA_GC Cuts at: 2712 2712 Size: 5926 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4117 4151 1903 Size: 34 2180 34 3678 Fragments arranged by size: 3678 2180 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2482 3818 5028 2482 Size: 1336 1210 3380 Fragments arranged by size: 3380 1336 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5926 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4537 136 Size: 107 122 71 631 605 2865 1525 Fragments arranged by size: 2865 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2023 2262 5921 2023 Size: 239 3659 2028 Fragments arranged by size: 3659 2028 239 BlpI GC'TnA_GC Cuts at: 3343 3343 Size: 5926 Bme1580I G_kGCm'C Cuts at: 1790 3987 5143 1790 Size: 2197 1156 2573 Fragments arranged by size: 2573 2197 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5926 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3127 3657 4615 Size: 416 53 83 399 24 1710 530 958 Cuts at: 4615 442 Size: 1753 Fragments arranged by size: 1753 1710 958 530 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5926 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2169 2169 Size: 5926 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2959 4586 1239 Size: 1720 1627 2579 Fragments arranged by size: 2579 1720 1627 Bpu10I CC'TnA_GC Cuts at: 2412 2712 2412 Size: 300 5626 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2889 4051 4916 5214 2889 Size: 1162 865 298 3601 Fragments arranged by size: 3601 1162 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2140 2447 2612 4589 882 Size: 617 641 307 165 1977 2219 Fragments arranged by size: 2219 1977 641 617 307 165 BsaAI yAC'GTr Cuts at: 493 1204 3717 493 Size: 711 2513 2702 Fragments arranged by size: 2702 2513 711 BsaBI GATnn'nnATC Cuts at: 2261 5920 2261 Size: 3659 2267 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2461 3733 4115 4750 Size: 53 83 186 936 928 1272 382 635 Cuts at: 4750 275 Size: 1451 Fragments arranged by size: 1451 1272 936 928 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4359 4757 5031 5178 5589 4359 Size: 398 274 147 411 4696 Fragments arranged by size: 4696 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5701 Fragments arranged by size: 5701 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5926 BseYI C'CCAG_C Cuts at: 5129 5625 5129 Size: 496 5430 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5353 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3376 4137 5165 1540 Size: 275 186 1375 761 1028 2301 Fragments arranged by size: 2301 1375 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2373 3987 4072 5143 2373 Size: 1614 85 1071 3156 Fragments arranged by size: 3156 1614 1071 85 BsmI GAATG_Cn' Cuts at: 3437 3530 3437 Size: 93 5833 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2373 3245 3987 4072 5143 1391 Size: 399 583 872 742 85 1071 2174 Fragments arranged by size: 2174 1071 872 742 583 399 85 BspEI T'CCGG_A Cuts at: 5589 5589 Size: 5926 BspHI T'CATG_A Cuts at: 3815 5545 3815 Size: 1730 4196 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2203 3281 844 Size: 1359 1078 3489 Fragments arranged by size: 3489 1359 1078 BsrBI CCG'CTC Cuts at: 1394 1823 3813 1394 Size: 429 1990 3507 Fragments arranged by size: 3507 1990 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2706 4421 4595 65 Size: 1183 1458 1715 174 1396 Fragments arranged by size: 1715 1458 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4570 4757 1700 Size: 110 2760 187 2869 Fragments arranged by size: 2869 2760 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5926 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5926 BssSI C'ACGA_G Cuts at: 2361 3986 4998 2361 Size: 1625 1012 3289 Fragments arranged by size: 3289 1625 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2100 2758 794 Size: 1306 658 3962 Fragments arranged by size: 3962 1306 658 BstBI TT'CG_AA Cuts at: 4730 4730 Size: 5926 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5926 BstYI r'GATC_y Cuts at: 1972 2023 2262 2349 3256 3623 4008 4025 5477 Size: 51 239 87 907 367 385 17 1452 Cuts at: 5477 5563 5575 5921 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 907 385 367 346 239 87 86 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2063 513 Size: 552 939 59 4376 Fragments arranged by size: 4376 939 552 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5187 Fragments arranged by size: 5187 683 56 BtsI GCAGTG_nn' Cuts at: 3442 4236 4256 3442 Size: 794 20 5112 Fragments arranged by size: 5112 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5926 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2052 2087 529 Size: 35 1488 35 4368 Fragments arranged by size: 4368 1488 35 35 DraI TTT'AAA Cuts at: 3230 3230 Size: 5926 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4933 809 Size: 507 3617 1802 Fragments arranged by size: 3617 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3373 4262 5891 Size: 54 1425 50 275 18 1543 889 1629 Cuts at: 5891 8 Size: 43 Fragments arranged by size: 1629 1543 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3373 1537 Size: 275 1561 4090 Fragments arranged by size: 4090 1561 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2444 3856 1353 Size: 1091 1412 3423 Fragments arranged by size: 3423 1412 1091 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4513 4887 5033 387 Size: 1064 138 2924 374 146 1280 Fragments arranged by size: 2924 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2172 2249 2959 3049 3989 4586 5373 Size: 393 540 77 710 90 940 597 787 Cuts at: 5373 5641 1239 Size: 268 1524 Fragments arranged by size: 1524 940 787 710 597 540 393 268 90 77 EcoRI G'AATT_C Cuts at: 2134 3235 3613 2134 Size: 1101 378 4447 Fragments arranged by size: 4447 1101 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3071 3071 Size: 5926 FspI TGC'GCA Cuts at: 4432 4432 Size: 5926 HaeII r_GCGC'y Cuts at: 1678 5073 1678 Size: 3395 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2446 2478 2763 2795 4567 Size: 32 135 32 874 32 285 32 1772 Cuts at: 4567 4599 4641 4673 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1772 874 285 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3270 3516 669 Size: 988 281 1332 246 3079 Fragments arranged by size: 3079 1332 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5926 HpaI GTT'AAC Cuts at: 3516 3516 Size: 5926 KpnI G_GTAC'C Cuts at: 3251 3251 Size: 5926 MfeI C'AATT_G Cuts at: 3525 3525 Size: 5926 MluI A'CGCG_T Cuts at: 5802 5802 Size: 5926 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5040 5224 1305 Size: 3735 184 2007 Fragments arranged by size: 3735 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4104 Fragments arranged by size: 4104 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2161 3884 4243 4402 518 Size: 648 995 1723 359 159 2042 Fragments arranged by size: 2042 1723 995 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2338 3342 4019 5167 5412 1606 Size: 732 1004 677 1148 245 2120 Fragments arranged by size: 2120 1148 1004 732 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4435 Fragments arranged by size: 4435 939 552 NdeI CA'TA_TG Cuts at: 387 2647 387 Size: 2260 3666 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5926 NotI GC'GGCC_GC Cuts at: 3373 3373 Size: 5926 NspI r_CATG'y Cuts at: 1132 1229 2034 2175 2384 3284 4829 1132 Size: 97 805 141 209 900 1545 2229 Fragments arranged by size: 2229 1545 900 805 209 141 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5926 PciI A'CATG_T Cuts at: 2380 4825 2380 Size: 2445 3481 PflMI CCAn_nnn'nTGG Cuts at: 1108 2859 1108 Size: 1751 4175 PmeI GTTT'AAAC Cuts at: 3230 3230 Size: 5926 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3995 4027 5508 5540 3995 Size: 32 1481 32 4381 Fragments arranged by size: 4381 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5926 PsiI TTA'TAA Cuts at: 2949 3496 2949 Size: 547 5379 PstI C_TGCA'G Cuts at: 830 2214 3278 830 Size: 1384 1064 3478 Fragments arranged by size: 3478 1384 1064 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5926 PvuII CAG'CTG Cuts at: 2338 2338 Size: 5926 SalI G'TCGA_C Cuts at: 1936 3268 1936 Size: 1332 4594 SbfI CC_TGCA'GG Cuts at: 2214 3278 2214 Size: 1064 4862 ScaI AGT'ACT Cuts at: 4174 5886 4174 Size: 1712 4214 SfcI C'TryA_G Cuts at: 826 1045 2210 2519 3274 4409 5090 5281 826 Size: 219 1165 309 755 1135 681 191 1471 Fragments arranged by size: 1471 1165 1135 755 681 309 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5926 SmaI CCC'GGG Cuts at: 1872 3253 1872 Size: 1381 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2904 4030 4931 5193 820 Size: 197 926 961 1126 901 262 1553 Fragments arranged by size: 1553 1126 961 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3717 493 Size: 3224 2702 SpeI A'CTAG_T Cuts at: 152 2501 2875 152 Size: 2349 374 3203 Fragments arranged by size: 3203 2349 374 SphI G_CATG'C Cuts at: 1132 3284 1132 Size: 2152 3774 SspI AAT'ATT Cuts at: 5 52 3850 5 Size: 47 3798 2081 Fragments arranged by size: 3798 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5926 StyI C'CwwG_G Cuts at: 513 1065 2004 2493 3365 513 Size: 552 939 489 872 3074 Fragments arranged by size: 3074 939 872 552 489 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3953 3970 4123 4308 4753 325 Size: 1255 2373 17 153 185 445 1498 Fragments arranged by size: 2373 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4172 5884 96 Size: 274 80 33 51 1443 2195 1712 138 Fragments arranged by size: 2195 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5886 5918 5886 Size: 32 5894 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5926 XbaI T'CTAG_A Cuts at: 3262 3262 Size: 5926 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5926 XmaI C'CCGG_G Cuts at: 1870 3251 1870 Size: 1381 4545 XmnI GAAnn'nnTTC Cuts at: 4055 4055 Size: 5926 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3734 276 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI