################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbsI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BpuAI BsaBI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI DraI DraIII DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI SacI SalI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp509I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AcyI 1047 GGCGCC G[GA]^CG[CT]C AocI 1030 CCTGAGG CC^T[ACGT]AGG AviII 776 TGCGCA TGC^GCA BbeI 1047 GGCGCC GGCGC^C BfaI 1369 CTAG C^TAG BglII 1448 AGATCT A^GATCT BsaHI 1047 GGCGCC G[GA]^CG[CT]C BsaI 1562 GAGACC GGTCTC(1/5) BsaWI 655 TCCGGT [AT]^CCGG[AT] BspLU11I 913 ACATGT A^CATGT BsrDI 1766 GCAATG GCAATG(2/0) Bsu36I 1030 CCTGAGG CC^T[ACGT]AGG CpoI 652 CGGTCCG CG^G[AT]CCG Eco81I 1030 CCTGAGG CC^T[ACGT]AGG FseI 1788 GGCCGGCC GGCCGG^CC FspI 776 TGCGCA TGC^GCA HgaI 1081 GCGTC GACGC(5/10) Hin1I 1047 GGCGCC G[GA]^CG[CT]C KasI 1047 GGCGCC G^GCGCC KspI 1239 CCGCGG CCGC^GG MaeI 1369 CTAG C^TAG MslI 319 CACCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 1047 GGCGCC GG^CGCC NcoI 141 CCATGG C^CATGG PvuII 227 CAGCTG CAG^CTG RsrII 652 CGGTCCG CG^G[AT]CCG SacII 1239 CCGCGG CCGC^GG SfoI 1047 GGCGCC GGC^GCC TfiI 1386 GATTC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AfaI 1344 GTAC GT^AC 1413 GTAC Alw44I 815 GTGCAC G^TGCAC 1491 GTGCAC ApaLI 815 GTGCAC G^TGCAC 1491 GTGCAC BalI 179 TGGCCA TGG^CCA 980 TGGCCA BbvCI 482 CCTCAGC CCTCAGC(-5/-2) 466 GCTGAGG BssHII 1470 GCGCGC G^CGCGC 1472 GCGCGC BstAPI 223 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1589 GCAAACAGTGC EarI 1222 CTCTTC CTCTTC(1/4) 745 GAAGAG EcoNI 433 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 735 CCTCCGCAAGG Ksp632I 1222 CTCTTC CTCTTC(1/4) 745 GAAGAG MluNI 179 TGGCCA TGG^CCA 980 TGGCCA MscI 179 TGGCCA TGG^CCA 980 TGGCCA NaeI 204 GCCGGC GCC^GGC 1789 GCCGGC NgoMIV 204 GCCGGC G^CCGGC 1789 GCCGGC NspI 913 ACATGT [GA]CATG^[CT] 1415 ACATGC PpuMI 188 AGGTCCC [GA]G^G[AT]CC[CT] 882 AGGTCCT PstI 559 CTGCAG CTGCA^G 669 CTGCAG RsaI 1344 GTAC GT^AC 1413 GTAC SapI 745 GAAGAGC GCTCTTC(1/4) 1221 GCTCTTC SmlI 94 CTCAAG C^T[CT][GA]AG 1486 CTCAAG StuI 853 AGGCCT AGG^CCT 1027 AGGCCT XcmI 318 CCACCAGTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1466 CCACGCGCGCGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccII AciI AflIII AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbrPI BbvI BcnI BglI BmyI BpmI BsaAI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PmaCI PmlI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SmaI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XhoII XmaCI XmaI