(Linear) (Six-base) MAPSORT of: KIEE4161.seq Check: 1755 from: 1 to: 1809 KIEE4161.orf With 182 enzymes: SgfI * July 3, 2012 18:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1504 1809 Size: 1504 305 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1437 1809 Size: 1437 372 AflIII A'CryG_T Cuts at: 0 260 913 1352 1622 1809 Size: 260 653 439 270 187 Fragments arranged by size: 653 439 270 260 187 AleI CACnn'nnGTG Cuts at: 0 323 1809 Size: 323 1486 AlwNI CAG_nnn'CTG Cuts at: 0 229 232 592 766 886 1395 1809 Size: 229 3 360 174 120 509 414 Fragments arranged by size: 509 414 360 229 174 120 3 ApaI G_GGCC'C Cuts at: 0 457 478 894 1256 1809 Size: 457 21 416 362 553 Fragments arranged by size: 553 457 416 362 21 ApaLI G'TGCA_C Cuts at: 0 815 1491 1809 Size: 815 676 318 Fragments arranged by size: 815 676 318 AvaI C'yCGr_G Cuts at: 0 280 754 893 1067 1429 1809 Size: 280 474 139 174 362 380 Fragments arranged by size: 474 380 362 280 174 139 BanI G'GyrC_C Cuts at: 0 35 52 1017 1047 1150 1640 1809 Size: 35 17 965 30 103 490 169 Fragments arranged by size: 965 490 169 103 35 30 17 BanII G_rGCy'C Cuts at: 0 457 478 812 894 1256 1809 Size: 457 21 334 82 362 553 Fragments arranged by size: 553 457 362 334 82 21 BbeI G_GCGC'C Cuts at: 0 1051 1809 Size: 1051 758 BbvCI CC'TCA_GC Cuts at: 0 467 483 1809 Size: 467 16 1326 Fragments arranged by size: 1326 467 16 BglI GCCn_nnn'nGGC Cuts at: 0 452 1251 1733 1790 1809 Size: 452 799 482 57 19 Fragments arranged by size: 799 482 452 57 19 BglII A'GATC_T Cuts at: 0 1448 1809 Size: 1448 361 Bme1580I G_kGCm'C Cuts at: 0 40 368 457 478 819 894 1256 1318 Size: 40 328 89 21 341 75 362 62 Cuts at: 1318 1495 1809 Size: 177 314 Fragments arranged by size: 362 341 328 314 177 89 75 62 40 21 BmgBI CAC'GTC Cuts at: 0 498 1101 1623 1809 Size: 498 603 522 186 Fragments arranged by size: 603 522 498 186 BmrI ACTGGGnnnn_n' Cuts at: 0 378 830 1761 1809 Size: 378 452 931 48 Fragments arranged by size: 931 452 378 48 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1585 1809 Size: 1585 224 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1216 1420 1575 1809 Size: 1216 204 155 234 Fragments arranged by size: 1216 234 204 155 Bpu10I CC'TnA_GC Cuts at: 0 113 467 483 1809 Size: 113 354 16 1326 Fragments arranged by size: 1326 354 113 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 1471 1809 Size: 79 1392 338 Fragments arranged by size: 1392 338 79 BsaI GGTCTCn'nnnn_ Cuts at: 0 1556 1809 Size: 1556 253 BsaAI yAC'GTr Cuts at: 0 263 1095 1353 1437 1809 Size: 263 832 258 84 372 Fragments arranged by size: 832 372 263 258 84 BsaHI Gr'CG_yC Cuts at: 0 1048 1809 Size: 1048 761 BsaWI w'CCGG_w Cuts at: 0 655 1809 Size: 655 1154 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 393 411 423 441 1162 1192 1809 Size: 393 18 12 18 721 30 617 Fragments arranged by size: 721 617 393 30 18 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 205 419 484 1183 1809 Size: 205 214 65 699 626 Fragments arranged by size: 699 626 214 205 65 BseYI C'CCAG_C Cuts at: 0 200 585 958 1809 Size: 200 385 373 851 Fragments arranged by size: 851 385 373 200 BsiEI CG_ry'CG Cuts at: 0 577 804 1274 1710 1794 1809 Size: 577 227 470 436 84 15 Fragments arranged by size: 577 470 436 227 84 15 BsiHKAI G_wGCw'C Cuts at: 0 819 1436 1495 1809 Size: 819 617 59 314 Fragments arranged by size: 819 617 314 59 Bsp1286I G_dGCh'C Cuts at: 0 40 368 457 478 812 819 894 1256 Size: 40 328 89 21 334 7 75 362 Cuts at: 1256 1318 1436 1495 1809 Size: 62 118 59 314 Fragments arranged by size: 362 334 328 314 118 89 75 62 59 40 21 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 512 1504 1673 1809 Size: 512 992 169 136 Fragments arranged by size: 992 512 169 136 BsrBI CCG'CTC Cuts at: 0 213 750 1569 1809 Size: 213 537 819 240 Fragments arranged by size: 819 537 240 213 BsrDI GCAATG_nn' Cuts at: 0 1773 1809 Size: 1773 36 BsrFI r'CCGG_y Cuts at: 0 204 487 624 1346 1789 1809 Size: 204 283 137 722 443 20 Fragments arranged by size: 722 443 283 204 137 20 BssHII G'CGCG_C Cuts at: 0 1470 1472 1809 Size: 1470 2 337 Fragments arranged by size: 1470 337 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 229 1595 1809 Size: 229 1366 214 Fragments arranged by size: 1366 229 214 BstXI CCAn_nnnn'nTGG Cuts at: 0 325 328 636 966 1809 Size: 325 3 308 330 843 Fragments arranged by size: 843 330 325 308 3 BstYI r'GATC_y Cuts at: 0 640 1373 1448 1809 Size: 640 733 75 361 Fragments arranged by size: 733 640 361 75 Bsu36I CC'TnA_GG Cuts at: 0 1031 1809 Size: 1031 778 BtgI C'CryG_G Cuts at: 0 6 141 689 1239 1809 Size: 6 135 548 550 570 Fragments arranged by size: 570 550 548 135 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 765 1592 1809 Size: 765 827 217 Fragments arranged by size: 827 765 217 BtsI GCAGTG_nn' Cuts at: 0 1286 1809 Size: 1286 523 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 158 193 1809 Size: 158 35 1616 Fragments arranged by size: 1616 158 35 EaeI y'GGCC_r Cuts at: 0 179 515 626 801 980 1271 1283 1478 Size: 179 336 111 175 179 291 12 195 Cuts at: 1478 1791 1809 Size: 313 18 Fragments arranged by size: 336 313 291 195 179 179 175 111 18 12 EagI C'GGCC_G Cuts at: 0 801 1271 1791 1809 Size: 801 470 520 18 Fragments arranged by size: 801 520 470 18 EarI CTCTTCn'nnn_ Cuts at: 0 740 1228 1809 Size: 740 488 581 Fragments arranged by size: 740 581 488 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 414 597 1003 1046 1336 1809 Size: 414 183 406 43 290 473 Fragments arranged by size: 473 414 406 290 183 43 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1216 1420 1437 1575 1809 Size: 1216 204 17 138 234 Fragments arranged by size: 1216 234 204 138 17 EcoNI CCTnn'n_nnAGG Cuts at: 0 437 739 1809 Size: 437 302 1070 Fragments arranged by size: 1070 437 302 EcoO109I rG'GnC_Cy Cuts at: 0 189 453 454 474 475 732 763 883 Size: 189 264 1 20 1 257 31 120 Cuts at: 883 890 1253 1809 Size: 7 363 556 Fragments arranged by size: 556 363 264 257 189 120 31 20 7 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 321 1809 Size: 321 1488 FseI GG_CCGG'CC Cuts at: 0 1793 1809 Size: 1793 16 FspI TGC'GCA Cuts at: 0 778 1809 Size: 778 1031 FspAI rTGC'GCAy Cuts at: 0 778 1809 Size: 778 1031 HaeII r_GCGC'y Cuts at: 0 408 592 1051 1809 Size: 408 184 459 758 Fragments arranged by size: 758 459 408 184 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 393 425 493 525 1809 Size: 393 32 68 32 1284 Fragments arranged by size: 1284 393 68 32 32 KasI G'GCGC_C Cuts at: 0 1047 1809 Size: 1047 762 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 821 1809 Size: 821 988 MscI TGG'CCA Cuts at: 0 181 982 1809 Size: 181 801 827 Fragments arranged by size: 827 801 181 MslI CAynn'nnrTG Cuts at: 0 323 1809 Size: 323 1486 MspA1I CmG'CkG Cuts at: 0 229 543 827 1241 1392 1637 1809 Size: 229 314 284 414 151 245 172 Fragments arranged by size: 414 314 284 245 229 172 151 NaeI GCC'GGC Cuts at: 0 206 1791 1809 Size: 206 1585 18 Fragments arranged by size: 1585 206 18 NarI GG'CG_CC Cuts at: 0 1048 1809 Size: 1048 761 NcoI C'CATG_G Cuts at: 0 141 1809 Size: 141 1668 NgoMIV G'CCGG_C Cuts at: 0 204 1789 1809 Size: 204 1585 20 Fragments arranged by size: 1585 204 20 NspI r_CATG'y Cuts at: 0 917 1419 1809 Size: 917 502 390 Fragments arranged by size: 917 502 390 PasI CC'CwG_GG Cuts at: 0 439 766 1809 Size: 439 327 1043 Fragments arranged by size: 1043 439 327 PciI A'CATG_T Cuts at: 0 913 1809 Size: 913 896 PflMI CCAn_nnn'nTGG Cuts at: 0 142 766 886 1809 Size: 142 624 120 923 Fragments arranged by size: 923 624 142 120 PfoI T'CCnGG_A Cuts at: 0 254 1809 Size: 254 1555 PmlI CAC'GTG Cuts at: 0 263 1095 1353 1437 1809 Size: 263 832 258 84 372 Fragments arranged by size: 832 372 263 258 84 PpuMI rG'GwC_Cy Cuts at: 0 189 883 1809 Size: 189 694 926 Fragments arranged by size: 926 694 189 PspOMI G'GGCC_C Cuts at: 0 453 474 890 1252 1809 Size: 453 21 416 362 557 Fragments arranged by size: 557 453 416 362 21 PstI C_TGCA'G Cuts at: 0 563 673 1809 Size: 563 110 1136 Fragments arranged by size: 1136 563 110 PvuII CAG'CTG Cuts at: 0 229 1809 Size: 229 1580 RsrII CG'GwC_CG Cuts at: 0 653 1809 Size: 653 1156 SacII CC_GC'GG Cuts at: 0 1242 1809 Size: 1242 567 SapI GCTCTTCn'nnn_ Cuts at: 0 740 1228 1809 Size: 740 488 581 Fragments arranged by size: 740 581 488 SfcI C'TryA_G Cuts at: 0 559 593 669 1809 Size: 559 34 76 1140 Fragments arranged by size: 1140 559 76 34 SfiI GGCCn_nnn'nGGCC Cuts at: 0 452 1251 1790 1809 Size: 452 799 539 19 Fragments arranged by size: 799 539 452 19 SfoI GGC'GCC Cuts at: 0 1049 1809 Size: 1049 760 SmaI CCC'GGG Cuts at: 0 282 756 1069 1809 Size: 282 474 313 740 Fragments arranged by size: 740 474 313 282 SmlI C'TyrA_G Cuts at: 0 94 1486 1809 Size: 94 1392 323 Fragments arranged by size: 1392 323 94 SrfI GCCC'GGGC Cuts at: 0 756 1809 Size: 756 1053 StuI AGG'CCT Cuts at: 0 855 1029 1809 Size: 855 174 780 Fragments arranged by size: 855 780 174 StyI C'CwwG_G Cuts at: 0 141 176 386 422 629 728 899 1809 Size: 141 35 210 36 207 99 171 910 Fragments arranged by size: 910 210 207 171 141 99 36 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 591 1743 1809 Size: 591 1152 66 Fragments arranged by size: 1152 591 66 TatI w'GTAC_w Cuts at: 0 1412 1809 Size: 1412 397 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 411 443 1162 1194 1809 Size: 411 32 719 32 615 Fragments arranged by size: 719 615 411 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 325 1473 1809 Size: 325 1148 336 Fragments arranged by size: 1148 336 325 XmaI C'CCGG_G Cuts at: 0 280 754 1067 1809 Size: 280 474 313 742 Fragments arranged by size: 742 474 313 280 Enzymes that do cut: AarI AcuI AflIII AleI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbvCI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FalI FseI FspI FspAI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII RsrII SacII SapI SfcI SfiI SfoI SmaI SmlI SrfI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI BlpI BmtI BsaBI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssSI BstBI BstEII BstZ17I ClaI DraI DraIII DrdI EcoICRI EcoRI EcoRV HincII HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PmeI PpiI PshAI PsiI PspXI PsrI PvuI SacI SalI SanDI SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SspI SwaI Tth111I XbaI XhoI XmnI ZraI