(Circular) (Six-base) MAPSORT of: pFN21AB6259.seq Check: 9617 from: 1 to: 6997 pFN21AB6259 6997 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2897 2897 Size: 6997 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4807 278 Size: 53 83 186 936 3271 2468 Fragments arranged by size: 3271 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2298 4340 1937 Size: 361 2042 4594 Fragments arranged by size: 4594 2042 361 Acc65I G'GTAC_C Cuts at: 4318 4318 Size: 6997 AclI AA'CG_TT Cuts at: 1563 5124 5497 1563 Size: 3561 373 3063 Fragments arranged by size: 3561 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3975 5060 6444 6712 1632 Size: 2343 1085 1384 268 1917 Fragments arranged by size: 2343 1917 1384 1085 268 AfeI AGC'GCT Cuts at: 2846 2846 Size: 6997 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6800 AflIII A'CryG_T Cuts at: 1203 5896 6873 1203 Size: 4693 977 1327 Fragments arranged by size: 4693 1327 977 AgeI A'CCGG_T Cuts at: 4089 5828 4089 Size: 1739 5258 AhdI GACnn_n'nnGTC Cuts at: 5726 5726 Size: 6997 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6997 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6997 AlwNI CAG_nnn'CTG Cuts at: 4740 6312 4740 Size: 1572 5425 ApaLI G'TGCA_C Cuts at: 5054 6210 5054 Size: 1156 5841 ApoI r'AATT_y Cuts at: 1493 2684 2882 3612 3702 4022 4097 4292 4306 Size: 1191 198 730 90 320 75 195 14 Cuts at: 4306 4532 4684 6793 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1191 730 320 226 198 195 152 90 75 14 AseI AT'TA_AT Cuts at: 160 1032 5551 160 Size: 872 4519 1606 Fragments arranged by size: 4519 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6997 AvaI C'yCGr_G Cuts at: 1870 1943 3455 4322 1870 Size: 73 1512 867 4545 Fragments arranged by size: 4545 1512 867 73 BamHI G'GATC_C Cuts at: 4327 4694 4327 Size: 367 6630 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3254 3301 4276 4318 5773 Size: 325 217 627 1467 47 975 42 1455 Cuts at: 5773 618 Size: 1842 Fragments arranged by size: 1842 1467 1455 975 627 325 217 47 42 BanII G_rGCy'C Cuts at: 1391 4316 1391 Size: 2925 4072 BbeI G_GCGC'C Cuts at: 3305 3305 Size: 6997 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2098 2777 928 Size: 971 199 679 5148 Fragments arranged by size: 5148 971 679 199 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5188 5222 1903 Size: 34 3251 34 3678 Fragments arranged by size: 3678 3251 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2830 2923 4889 6099 2830 Size: 93 1966 1210 3728 Fragments arranged by size: 3728 1966 1210 93 BclI T'GATC_A Cuts at: 1548 1548 Size: 6997 BfrBI ATG'CAT Cuts at: 2327 2327 Size: 6997 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5608 136 Size: 107 122 71 631 605 3936 1525 Fragments arranged by size: 3936 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6992 6992 Size: 6997 BlpI GC'TnA_GC Cuts at: 4414 4414 Size: 6997 Bme1580I G_kGCm'C Cuts at: 1790 2871 5058 6214 1790 Size: 1081 2187 1156 2573 Fragments arranged by size: 2573 2187 1156 1081 BmgBI CAC'GTC Cuts at: 1318 3001 1318 Size: 1683 5314 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2303 3152 4197 Size: 416 53 83 399 24 886 849 1045 Cuts at: 4197 4728 5686 442 Size: 531 958 1753 Fragments arranged by size: 1753 1045 958 886 849 531 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6997 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3181 5657 1239 Size: 1942 2476 2579 Fragments arranged by size: 2579 2476 1942 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5122 5987 6285 5122 Size: 865 298 5834 Fragments arranged by size: 5834 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5660 882 Size: 617 4161 2219 Fragments arranged by size: 4161 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4259 4788 493 Size: 711 3055 529 2702 Fragments arranged by size: 3055 2702 711 529 BsaBI GATnn'nnATC Cuts at: 3117 3625 6991 3117 Size: 508 3366 3123 Fragments arranged by size: 3366 3123 508 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3302 4804 5186 5821 Size: 53 83 186 936 1769 1502 382 635 Cuts at: 5821 275 Size: 1451 Fragments arranged by size: 1769 1502 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4089 5430 5828 6102 6249 6660 4089 Size: 1341 398 274 147 411 4426 Fragments arranged by size: 4426 1341 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6772 Fragments arranged by size: 6772 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6997 BseYI C'CCAG_C Cuts at: 2338 3179 3242 6200 6696 2338 Size: 841 63 2958 496 2639 Fragments arranged by size: 2958 2639 841 496 63 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6424 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4447 5208 6236 1540 Size: 275 186 2446 761 1028 2301 Fragments arranged by size: 2446 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3221 5058 5143 6214 3221 Size: 1837 85 1071 4004 Fragments arranged by size: 4004 1837 1071 85 BsmI GAATG_Cn' Cuts at: 3360 4508 4601 3360 Size: 1148 93 5756 Fragments arranged by size: 5756 1148 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2871 3221 4316 5058 5143 6214 1391 Size: 399 1081 350 1095 742 85 1071 2174 Fragments arranged by size: 2174 1095 1081 1071 742 399 350 85 BspEI T'CCGG_A Cuts at: 6660 6660 Size: 6997 BspHI T'CATG_A Cuts at: 3423 4886 6616 3423 Size: 1463 1730 3804 Fragments arranged by size: 3804 1730 1463 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2897 3885 4352 844 Size: 2053 988 467 3489 Fragments arranged by size: 3489 2053 988 467 BsrBI CCG'CTC Cuts at: 1394 1823 4884 1394 Size: 429 3061 3507 Fragments arranged by size: 3507 3061 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2467 3051 5492 5666 65 Size: 1183 1219 584 2441 174 1396 Fragments arranged by size: 2441 1396 1219 1183 584 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2205 4089 5641 5828 1700 Size: 110 395 1884 1552 187 2869 Fragments arranged by size: 2869 1884 1552 395 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6997 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6997 BssSI C'ACGA_G Cuts at: 5057 6069 5057 Size: 1012 5985 BstAPI GCAn_nnn'nTGC Cuts at: 794 3050 3760 794 Size: 2256 710 4031 Fragments arranged by size: 4031 2256 710 BstBI TT'CG_AA Cuts at: 3700 5801 3700 Size: 2101 4896 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6997 BstXI CCAn_nnnn'nTGG Cuts at: 3448 3448 Size: 6997 BstYI r'GATC_y Cuts at: 1972 4327 4694 5079 5096 6548 6634 6646 6992 Size: 2355 367 385 17 1452 86 12 346 Cuts at: 6992 1972 Size: 1977 Fragments arranged by size: 2355 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2382 513 Size: 552 1317 5128 Fragments arranged by size: 5128 1317 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3025 487 Size: 683 56 1799 4459 Fragments arranged by size: 4459 1799 683 56 BtsI GCAGTG_nn' Cuts at: 3066 3975 4513 5307 5327 3066 Size: 909 538 794 20 4736 Fragments arranged by size: 4736 909 794 538 20 ClaI AT'CG_AT Cuts at: 1554 3675 4010 1554 Size: 2121 335 4541 Fragments arranged by size: 4541 2121 335 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6962 DraI TTT'AAA Cuts at: 2932 2944 4301 2932 Size: 12 1357 5628 Fragments arranged by size: 5628 1357 12 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2707 6004 809 Size: 507 1391 3297 1802 Fragments arranged by size: 3297 1802 1391 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2262 4444 Size: 54 1425 50 275 18 207 225 2182 Cuts at: 4444 5333 6962 8 Size: 889 1629 43 Fragments arranged by size: 2182 1629 1425 889 275 225 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4444 1537 Size: 275 2632 4090 Fragments arranged by size: 4090 2632 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3816 4927 1353 Size: 2463 1111 3423 Fragments arranged by size: 3423 2463 1111 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3679 5584 5958 6104 387 Size: 1064 138 2090 1905 374 146 1280 Fragments arranged by size: 2090 1905 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3181 3975 5060 5657 6444 6712 1239 Size: 393 1549 794 1085 597 787 268 1524 Fragments arranged by size: 1549 1524 1085 794 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3510 3925 4000 3510 Size: 415 75 6507 Fragments arranged by size: 6507 415 75 EcoRI G'AATT_C Cuts at: 2882 3702 4306 4684 2882 Size: 820 604 378 5195 Fragments arranged by size: 5195 820 604 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2876 3775 2876 Size: 899 6098 FspI TGC'GCA Cuts at: 5503 5503 Size: 6997 HaeII r_GCGC'y Cuts at: 1678 2848 3305 6144 1678 Size: 1170 457 2839 2531 Fragments arranged by size: 2839 2531 1170 457 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5638 5670 5712 5744 1373 Size: 32 135 32 4066 32 42 32 2626 Fragments arranged by size: 4066 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2299 2700 3799 4341 4587 669 Size: 988 281 361 401 1099 542 246 3079 Fragments arranged by size: 3079 1099 988 542 401 361 281 246 HindIII A'AGCT_T Cuts at: 748 3723 4082 748 Size: 2975 359 3663 Fragments arranged by size: 3663 2975 359 HpaI GTT'AAC Cuts at: 4587 4587 Size: 6997 KasI G'GCGC_C Cuts at: 3301 3301 Size: 6997 KpnI G_GTAC'C Cuts at: 4322 4322 Size: 6997 MfeI C'AATT_G Cuts at: 4596 4596 Size: 6997 MluI A'CGCG_T Cuts at: 6873 6873 Size: 6997 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4036 6111 6295 1305 Size: 2731 2075 184 2007 Fragments arranged by size: 2731 2075 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2039 10 Size: 54 1425 343 207 4968 Fragments arranged by size: 4968 1425 343 207 54 MslI CAynn'nnrTG Cuts at: 518 1166 2381 3044 3263 4955 5314 5473 518 Size: 648 1215 663 219 1692 359 159 2042 Fragments arranged by size: 2042 1692 1215 663 648 359 219 159 MspA1I CmG'CkG Cuts at: 1606 4413 5090 6238 6483 1606 Size: 2807 677 1148 245 2120 Fragments arranged by size: 2807 2120 1148 677 245 NarI GG'CG_CC Cuts at: 3302 3302 Size: 6997 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6445 NdeI CA'TA_TG Cuts at: 387 387 Size: 6997 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6997 NotI GC'GGCC_GC Cuts at: 4444 4444 Size: 6997 NsiI A_TGCA'T Cuts at: 2329 2329 Size: 6997 NspI r_CATG'y Cuts at: 1132 1229 4355 5900 1132 Size: 97 3126 1545 2229 Fragments arranged by size: 3126 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6997 PciI A'CATG_T Cuts at: 5896 5896 Size: 6997 PflMI CCAn_nnn'nTGG Cuts at: 1108 2315 3113 1108 Size: 1207 798 4992 Fragments arranged by size: 4992 1207 798 PfoI T'CCnGG_A Cuts at: 2971 2971 Size: 6997 PmeI GTTT'AAAC Cuts at: 4301 4301 Size: 6997 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5066 5098 6579 6611 5066 Size: 32 1481 32 5452 Fragments arranged by size: 5452 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3510 3925 3510 Size: 415 6582 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6997 PsiI TTA'TAA Cuts at: 4567 4567 Size: 6997 PstI C_TGCA'G Cuts at: 830 3750 4349 830 Size: 2920 599 3478 Fragments arranged by size: 3478 2920 599 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6997 SalI G'TCGA_C Cuts at: 1936 2297 4339 1936 Size: 361 2042 4594 Fragments arranged by size: 4594 2042 361 SapI GCTCTTCn'nnn_ Cuts at: 3816 3816 Size: 6997 SbfI CC_TGCA'GG Cuts at: 4349 4349 Size: 6997 ScaI AGT'ACT Cuts at: 2742 3187 3849 4186 5245 6957 2742 Size: 445 662 337 1059 1712 2782 Fragments arranged by size: 2782 1712 1059 662 445 337 SexAI A'CCwGG_T Cuts at: 2787 2787 Size: 6997 SfcI C'TryA_G Cuts at: 826 1045 3746 4345 5480 6161 6352 826 Size: 219 2701 599 1135 681 191 1471 Fragments arranged by size: 2701 1471 1135 681 599 219 191 SfoI GGC'GCC Cuts at: 3303 3303 Size: 6997 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6997 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6997 SmaI CCC'GGG Cuts at: 1872 4324 1872 Size: 2452 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5101 6002 6264 820 Size: 197 926 3158 901 262 1553 Fragments arranged by size: 3158 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4259 4788 493 Size: 3766 529 2702 Fragments arranged by size: 3766 2702 529 SpeI A'CTAG_T Cuts at: 152 152 Size: 6997 SphI G_CATG'C Cuts at: 1132 4355 1132 Size: 3223 3774 SspI AAT'ATT Cuts at: 5 52 2779 3544 4128 4921 5 Size: 47 2727 765 584 793 2081 Fragments arranged by size: 2727 2081 793 765 584 47 StuI AGG'CCT Cuts at: 1517 3465 1517 Size: 1948 5049 StyI C'CwwG_G Cuts at: 513 1065 3135 4436 513 Size: 552 2070 1301 3074 Fragments arranged by size: 3074 2070 1301 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5024 5041 5194 5379 5824 325 Size: 1255 3444 17 153 185 445 1498 Fragments arranged by size: 3444 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2740 3185 3791 Size: 274 80 33 51 1443 763 445 606 Cuts at: 3791 3847 4184 5243 6955 96 Size: 56 337 1059 1712 138 Fragments arranged by size: 1712 1443 1059 763 606 445 337 274 138 80 56 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2592 2624 6957 6989 2592 Size: 32 4333 32 2600 Fragments arranged by size: 4333 2600 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6997 XbaI T'CTAG_A Cuts at: 4333 4333 Size: 6997 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2579 2579 Size: 6997 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6997 XmaI C'CCGG_G Cuts at: 1870 4322 1870 Size: 2452 4545 XmnI GAAnn'nnTTC Cuts at: 3614 5126 3614 Size: 1512 5485 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4805 276 Size: 53 83 186 936 3271 2468 Fragments arranged by size: 3271 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SrfI SwaI