Restriction map for
pFN21AB6918
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KspI MaeI
MaeIII MamI MfeI MluI MroI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI Tsp45I TspRI
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 546 GGTACC G^GTACC
AlwI 74 GATCC GGATC(4/5)
AocI 424 CCTCAGG CC^T[ACGT]AGG
Asp718I 546 GGTACC G^GTACC
AvaII 429 GGTCC G^G[AT]CC
BalI 372 TGGCCA TGG^CCA
BanI 546 GGTACC G^G[CT][GA]CC
BanII 177 GGGCTC G[GA]GC[CT]^C
BbvI 604 GCTGC GCAGC(8/12)
BmyI 177 GGGCTC G[AGT]GC[ACT]^C
BpmI 599 CTGGAG CTGGAG(16/14)
BsiYI 217 CCATTTTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 217 CCATTTTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 177 GGGCTC G[AGT]GC[ACT]^C
BsrDI 564 CATTGC GCAATG(2/0)
BsrI 174 ACTGG ACTGG(1/-1)
BstXI 329 CCAAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 73 AGATCC [GA]^GATC[CT]
Bsu36I 424 CCTCAGG CC^T[ACGT]AGG
Cfr13I 429 GGTCC G^G[ACGT]CC
DraI 126 TTTAAA TTT^AAA
DraII 428 AGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 372 TGGCCA [CT]^GGCC[GA]
Eco81I 424 CCTCAGG CC^T[ACGT]AGG
EcoO109I 428 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 4 GATATC GAT^ATC
EcoT14I 402 CCAAGG C^C[AT][AT]GG
Fnu4HI 604 GCTGC GC^[ACGT]GC
HinfI 259 GATTC G^A[ACGT]TC
HphI 659 TCACC GGTGA(8/7)
ItaI 604 GCTGC GC^[ACGT]GC
KpnI 546 GGTACC GGTAC^C
MaeII 540 ACGT A^CGT
MflI 73 AGATCC [GA]^GATC[CT]
MluNI 372 TGGCCA TGG^CCA
MscI 372 TGGCCA TGG^CCA
MslI 135 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 217 CCATTTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 428 AGGTCCC [GA]G^G[AT]CC[CT]
Sau96I 429 GGTCC G^G[ACGT]CC
SfaNI 613 GATGC GCATC(5/9)
SmlI 195 CTTGAG C^T[CT][GA]AG
StuI 152 AGGCCT AGG^CCT
StyI 402 CCAAGG C^C[AT][AT]GG
TfiI 259 GATTC G^A[AT]TC
TseI 604 GCTGC G^C[AT]GC
Van91I 217 CCATTTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 73 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
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AfaI 538 GTAC GT^AC
547 GTAC
BsmAI 198 GAGAC GTCTC(1/5)
481 GAGAC
BsmFI 430 GTCCC GGGAC(10/14)
642 GTCCC
BstF5I 182 CATCC GGATG(2/0)
612 GGATG
Cac8I 150 GCAGGC GC[ACGT]^[ACGT]GC
594 GCAGGC
DdeI 95 CTCAG C^T[ACGT]AG
425 CTCAG
EarI 69 GAAGAG CTCTTC(1/4)
478 GAAGAG
FokI 182 CATCC GGATG(9/13)
612 GGATG
HaeIII 153 GGCC GG^CC
373 GGCC
Ksp632I 69 GAAGAG CTCTTC(1/4)
478 GAAGAG
MwoI 598 GCTGGAGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
607 GCCAGGGATGC
NlaIV 429 GGTCCC GG[ACGT]^[ACGT]CC
546 GGTACC
RsaI 538 GTAC GT^AC
547 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AcsI AluI ApoI BsaJI BssKI
BstNI DpnII EcoRII MboI MboII
MnlI MseI MvaI NdeII NlaIII
Sau3AI ScrFI Tru9I Tsp509I