(Circular) (Six-base) MAPSORT of: pFN21AE9630.seq Check: 9906 from: 1 to: 7960 pFN21AE9630 7960 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4043 4043 Size: 7960 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5770 278 Size: 53 83 186 936 4234 2468 Fragments arranged by size: 4234 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3827 5303 1937 Size: 1890 1476 4594 Fragments arranged by size: 4594 1890 1476 Acc65I G'GTAC_C Cuts at: 5281 5281 Size: 7960 AclI AA'CG_TT Cuts at: 1563 6087 6460 1563 Size: 4524 373 3063 Fragments arranged by size: 4524 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2012 2354 2583 3741 5070 5186 6023 7407 Size: 380 342 229 1158 1329 116 837 1384 Cuts at: 7407 7675 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1329 1158 837 380 342 268 229 116 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7763 AflIII A'CryG_T Cuts at: 1203 4011 4545 6859 7836 1203 Size: 2808 534 2314 977 1327 Fragments arranged by size: 2808 2314 1327 977 534 AgeI A'CCGG_T Cuts at: 6791 6791 Size: 7960 AhdI GACnn_n'nnGTC Cuts at: 6689 6689 Size: 7960 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7960 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2376 1184 Size: 1192 6768 AlwNI CAG_nnn'CTG Cuts at: 3007 4112 5703 7275 3007 Size: 1105 1591 1572 3692 Fragments arranged by size: 3692 1591 1572 1105 ApaI G_GGCC'C Cuts at: 5209 5209 Size: 7960 ApaLI G'TGCA_C Cuts at: 2666 3389 3860 6017 7173 2666 Size: 723 471 2157 1156 3453 Fragments arranged by size: 3453 2157 1156 723 471 ApoI r'AATT_y Cuts at: 1493 2113 2168 2225 2379 2747 3245 3962 4340 Size: 620 55 57 154 368 498 717 378 Cuts at: 4340 4454 4493 5093 5269 5495 5647 7756 1493 Size: 114 39 600 176 226 152 2109 1697 Fragments arranged by size: 2109 1697 717 620 600 498 378 368 226 176 154 152 114 57 55 39 AseI AT'TA_AT Cuts at: 160 1032 6514 160 Size: 872 5482 1606 Fragments arranged by size: 5482 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7960 AvaI C'yCGr_G Cuts at: 1870 1943 5285 1870 Size: 73 3342 4545 Fragments arranged by size: 4545 3342 73 BamHI G'GATC_C Cuts at: 5290 5657 5290 Size: 367 7593 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3076 3775 3943 5281 6736 Size: 325 217 627 1289 699 168 1338 1455 Cuts at: 6736 618 Size: 1842 Fragments arranged by size: 1842 1455 1338 1289 699 627 325 217 168 BanII G_rGCy'C Cuts at: 1391 2773 3794 4158 4608 5209 5279 1391 Size: 1382 1021 364 450 601 70 4072 Fragments arranged by size: 4072 1382 1021 601 450 364 70 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2823 3014 928 Size: 971 924 191 5874 Fragments arranged by size: 5874 971 924 191 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6151 6185 1903 Size: 34 4214 34 3678 Fragments arranged by size: 4214 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5852 7062 5852 Size: 1210 6750 BclI T'GATC_A Cuts at: 1548 2637 1548 Size: 1089 6871 BfrBI ATG'CAT Cuts at: 3500 3500 Size: 7960 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6571 136 Size: 107 122 71 631 605 4899 1525 Fragments arranged by size: 4899 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4426 7955 4426 Size: 3529 4431 BlpI GC'TnA_GC Cuts at: 4934 5377 4934 Size: 443 7517 Bme1580I G_kGCm'C Cuts at: 1790 2670 3393 3864 5209 6021 7177 1790 Size: 880 723 471 1345 812 1156 2573 Fragments arranged by size: 2573 1345 1156 880 812 723 471 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7960 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3350 3505 3774 Size: 416 53 83 399 24 1933 155 269 Cuts at: 3774 4504 5691 6649 442 Size: 730 1187 958 1753 Fragments arranged by size: 1933 1753 1187 958 730 416 399 269 155 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7960 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3168 3168 Size: 7960 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2607 6620 1239 Size: 1368 4013 2579 Fragments arranged by size: 4013 2579 1368 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2451 4659 6085 6950 7248 2451 Size: 2208 1426 865 298 3163 Fragments arranged by size: 3163 2208 1426 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6623 882 Size: 617 5124 2219 Fragments arranged by size: 5124 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2975 5213 5751 493 Size: 711 1771 2238 538 2702 Fragments arranged by size: 2702 2238 1771 711 538 BsaBI GATnn'nnATC Cuts at: 5127 7954 5127 Size: 2827 5133 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5767 6149 6784 275 Size: 53 83 186 936 4234 382 635 1451 Fragments arranged by size: 4234 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6393 6791 7065 7212 7623 6393 Size: 398 274 147 411 6730 Fragments arranged by size: 6730 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7735 Fragments arranged by size: 7735 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3144 5049 1186 Size: 1958 1905 4097 Fragments arranged by size: 4097 1958 1905 BseYI C'CCAG_C Cuts at: 2997 3130 4151 7163 7659 2997 Size: 133 1021 3012 496 3298 Fragments arranged by size: 3298 3012 1021 496 133 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3392 3798 1163 Size: 573 1656 406 5325 Fragments arranged by size: 5325 1656 573 406 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5410 6171 7199 1540 Size: 275 186 3409 761 1028 2301 Fragments arranged by size: 3409 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2670 3393 3864 4608 6021 6106 7177 2670 Size: 723 471 744 1413 85 1071 3453 Fragments arranged by size: 3453 1413 1071 744 723 471 85 BsmI GAATG_Cn' Cuts at: 4710 5471 5564 4710 Size: 761 93 7106 Fragments arranged by size: 7106 761 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4011 4011 Size: 7960 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2670 2773 3393 3794 3864 4158 4608 Size: 399 880 103 620 401 70 294 450 Cuts at: 4608 5209 5279 6021 6106 7177 1391 Size: 601 70 742 85 1071 2174 Fragments arranged by size: 2174 1071 880 742 620 601 450 401 399 294 103 85 70 70 BspEI T'CCGG_A Cuts at: 7623 7623 Size: 7960 BspHI T'CATG_A Cuts at: 3241 3417 5849 7579 3241 Size: 176 2432 1730 3622 Fragments arranged by size: 3622 2432 1730 176 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2692 4043 5315 844 Size: 1848 1351 1272 3489 Fragments arranged by size: 3489 1848 1351 1272 BsrBI CCG'CTC Cuts at: 1394 1823 5847 1394 Size: 429 4024 3507 Fragments arranged by size: 4024 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2071 2310 2810 3147 4122 4313 6455 Size: 1183 823 239 500 337 975 191 2142 Cuts at: 6455 6629 65 Size: 174 1396 Fragments arranged by size: 2142 1396 1183 975 823 500 337 239 191 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6604 6791 1700 Size: 110 4794 187 2869 Fragments arranged by size: 4794 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2271 2307 96 Size: 2175 36 5749 Fragments arranged by size: 5749 2175 36 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7960 BssSI C'ACGA_G Cuts at: 6020 7032 6020 Size: 1012 6948 BstAPI GCAn_nnn'nTGC Cuts at: 794 3821 794 Size: 3027 4933 BstBI TT'CG_AA Cuts at: 6764 6764 Size: 7960 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7960 BstXI CCAn_nnnn'nTGG Cuts at: 2285 2285 Size: 7960 BstYI r'GATC_y Cuts at: 1972 2932 4132 4426 5290 5657 6042 6059 7511 Size: 960 1200 294 864 367 385 17 1452 Cuts at: 7511 7597 7609 7955 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1200 960 864 385 367 346 294 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2278 4226 4946 513 Size: 552 939 274 1948 720 3527 Fragments arranged by size: 3527 1948 939 720 552 274 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7221 Fragments arranged by size: 7221 683 56 BtsI GCAGTG_nn' Cuts at: 3207 4594 5476 6270 6290 3207 Size: 1387 882 794 20 4877 Fragments arranged by size: 4877 1387 882 794 20 ClaI AT'CG_AT Cuts at: 1554 4962 1554 Size: 3408 4552 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7925 DraI TTT'AAA Cuts at: 2223 2508 4464 5264 2223 Size: 285 1956 800 4919 Fragments arranged by size: 4919 1956 800 285 DraIII CAC_nnn'GTG Cuts at: 3260 3260 Size: 7960 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6967 809 Size: 507 5651 1802 Fragments arranged by size: 5651 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3204 5407 6296 Size: 54 1425 50 275 18 1374 2203 889 Cuts at: 6296 7925 8 Size: 1629 43 Fragments arranged by size: 2203 1629 1425 1374 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5407 1537 Size: 275 3595 4090 Fragments arranged by size: 4090 3595 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2103 2626 3571 3717 5890 1353 Size: 750 523 945 146 2173 3423 Fragments arranged by size: 3423 2173 945 750 523 146 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6547 6921 7067 387 Size: 1064 138 4958 374 146 1280 Fragments arranged by size: 4958 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4606 4606 Size: 7960 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2012 2354 2583 2607 3741 5070 5186 Size: 393 380 342 229 24 1134 1329 116 Cuts at: 5186 6023 6620 7407 7675 1239 Size: 837 597 787 268 1524 Fragments arranged by size: 1524 1329 1134 837 787 597 393 380 342 268 229 116 24 EcoNI CCTnn'n_nnAGG Cuts at: 4050 4050 Size: 7960 EcoO109I rG'GnC_Cy Cuts at: 3657 4506 5205 5206 3657 Size: 849 699 1 6411 Fragments arranged by size: 6411 849 699 1 EcoRI G'AATT_C Cuts at: 2379 3245 4493 5269 5647 2379 Size: 866 1248 776 378 4692 Fragments arranged by size: 4692 1248 866 776 378 EcoRV GAT'ATC Cuts at: 2595 2595 Size: 7960 FspI TGC'GCA Cuts at: 6466 6466 Size: 7960 HaeII r_GCGC'y Cuts at: 1678 3017 7107 1678 Size: 1339 4090 2531 Fragments arranged by size: 4090 2531 1339 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2600 2632 3255 3287 3791 Size: 32 135 32 1028 32 623 32 504 Cuts at: 3791 3823 4270 4302 4802 4834 6601 6633 6675 Size: 32 447 32 500 32 1767 32 42 Cuts at: 6675 6707 1373 Size: 32 2626 Fragments arranged by size: 2626 1767 1028 623 504 500 447 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5304 5550 669 Size: 988 281 3366 246 3079 Fragments arranged by size: 3366 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2726 3842 748 Size: 1978 1116 4866 Fragments arranged by size: 4866 1978 1116 HpaI GTT'AAC Cuts at: 5550 5550 Size: 7960 KpnI G_GTAC'C Cuts at: 5285 5285 Size: 7960 MfeI C'AATT_G Cuts at: 4346 5559 4346 Size: 1213 6747 MluI A'CGCG_T Cuts at: 7836 7836 Size: 7960 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7074 7258 1305 Size: 5769 184 2007 Fragments arranged by size: 5769 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3206 10 Size: 54 1425 343 1374 4764 Fragments arranged by size: 4764 1425 1374 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2358 3422 3941 4550 4734 5012 5018 Size: 648 1192 1064 519 609 184 278 6 Cuts at: 5018 5918 6277 6436 518 Size: 900 359 159 2042 Fragments arranged by size: 2042 1192 1064 900 648 609 519 359 278 184 159 6 MspA1I CmG'CkG Cuts at: 1606 5376 6053 7201 7446 1606 Size: 3770 677 1148 245 2120 Fragments arranged by size: 3770 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2278 4226 4946 513 Size: 552 939 274 1948 720 3527 Fragments arranged by size: 3527 1948 939 720 552 274 NdeI CA'TA_TG Cuts at: 387 387 Size: 7960 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7960 NotI GC'GGCC_GC Cuts at: 5407 5407 Size: 7960 NsiI A_TGCA'T Cuts at: 3502 3502 Size: 7960 NspI r_CATG'y Cuts at: 1132 1229 2050 2357 4015 4549 4999 5318 6863 Size: 97 821 307 1658 534 450 319 1545 Cuts at: 6863 1132 Size: 2229 Fragments arranged by size: 2229 1658 1545 821 534 450 319 307 97 PasI CC'CwG_GG Cuts at: 1348 4420 4488 1348 Size: 3072 68 4820 Fragments arranged by size: 4820 3072 68 PciI A'CATG_T Cuts at: 4011 4545 6859 4011 Size: 534 2314 5112 Fragments arranged by size: 5112 2314 534 PflMI CCAn_nnn'nTGG Cuts at: 1108 3260 4488 4516 1108 Size: 2152 1228 28 4552 Fragments arranged by size: 4552 2152 1228 28 PfoI T'CCnGG_A Cuts at: 2536 2536 Size: 7960 PmeI GTTT'AAAC Cuts at: 5264 5264 Size: 7960 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6029 6061 7542 7574 6029 Size: 32 1481 32 6415 Fragments arranged by size: 6415 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3657 4506 3657 Size: 849 7111 PshAI GACnn'nnGTC Cuts at: 1613 4845 1613 Size: 3232 4728 PsiI TTA'TAA Cuts at: 5530 5530 Size: 7960 PspOMI G'GGCC_C Cuts at: 5205 5205 Size: 7960 PstI C_TGCA'G Cuts at: 830 2352 3118 4054 5312 830 Size: 1522 766 936 1258 3478 Fragments arranged by size: 3478 1522 1258 936 766 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7960 SacI G_AGCT'C Cuts at: 4608 4608 Size: 7960 SalI G'TCGA_C Cuts at: 1936 5302 1936 Size: 3366 4594 SanDI GG'GwC_CC Cuts at: 4506 4506 Size: 7960 SbfI CC_TGCA'GG Cuts at: 5312 5312 Size: 7960 ScaI AGT'ACT Cuts at: 3238 6208 7920 3238 Size: 2970 1712 3278 Fragments arranged by size: 3278 2970 1712 SfcI C'TryA_G Cuts at: 826 1045 2348 3114 4050 4609 5308 6443 7124 Size: 219 1303 766 936 559 699 1135 681 Cuts at: 7124 7315 826 Size: 191 1471 Fragments arranged by size: 1471 1303 1135 936 766 699 681 559 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7960 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7960 SmaI CCC'GGG Cuts at: 1872 5287 1872 Size: 3415 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2466 4674 6064 6965 7227 820 Size: 197 926 523 2208 1390 901 262 1553 Fragments arranged by size: 2208 1553 1390 926 901 523 262 197 SnaBI TAC'GTA Cuts at: 493 5213 5751 493 Size: 4720 538 2702 Fragments arranged by size: 4720 2702 538 SpeI A'CTAG_T Cuts at: 152 152 Size: 7960 SphI G_CATG'C Cuts at: 1132 2357 4999 5318 1132 Size: 1225 2642 319 3774 Fragments arranged by size: 3774 2642 1225 319 SspI AAT'ATT Cuts at: 5 52 2660 5116 5884 5 Size: 47 2608 2456 768 2081 Fragments arranged by size: 2608 2456 2081 768 47 StuI AGG'CCT Cuts at: 1517 3998 1517 Size: 2481 5479 StyI C'CwwG_G Cuts at: 513 1065 2004 2278 2338 4226 4946 5399 513 Size: 552 939 274 60 1888 720 453 3074 Fragments arranged by size: 3074 1888 939 720 552 453 274 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3397 3793 5987 6004 6157 6342 6787 Size: 1255 1817 396 2194 17 153 185 445 Cuts at: 6787 325 Size: 1498 Fragments arranged by size: 2194 1817 1498 1255 445 396 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2235 2271 2307 Size: 274 80 33 51 1443 258 36 36 Cuts at: 2307 3236 5227 6206 7918 96 Size: 929 1991 979 1712 138 Fragments arranged by size: 1991 1712 1443 979 929 274 258 138 80 51 36 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7920 7952 7920 Size: 32 7928 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7960 XbaI T'CTAG_A Cuts at: 3481 5296 3481 Size: 1815 6145 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3199 4379 3199 Size: 1180 6780 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7960 XmaI C'CCGG_G Cuts at: 1870 5285 1870 Size: 3415 4545 XmnI GAAnn'nnTTC Cuts at: 4743 5097 6089 4743 Size: 354 992 6614 Fragments arranged by size: 6614 992 354 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5768 276 Size: 53 83 186 936 4234 2468 Fragments arranged by size: 4234 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstZ17I Bsu36I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI