################################## # NO CUT ENZYMES ( 194 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbsI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TspRI Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccI 32 GTCTAC GT^[AC][GT]AC AcsI 334 GAATTT [GA]^AATT[CT] AlwNI 395 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 334 GAATTT [GA]^AATT[CT] BanI 259 GGTGCC G^G[CT][GA]CC BbrPI 328 CACGTG CAC^GTG BbvCI 468 CCTCAGC CCTCAGC(-5/-2) BciVI 75 GGATAC GTATCC(6/5) BsaAI 328 CACGTG [CT]AC^GT[GA] BsgI 392 GTGCAG GTGCAG(16/14) BspMI 386 GCAGGT ACCTGC(4/8) BsrI 432 CCAGT ACTGG(1/-1) DdeI 469 CTCAG C^T[ACGT]AG DraI 25 TTTAAA TTT^AAA DraII 464 AGGACCT [GA]G^G[ACGT]CC[CT] DsaI 451 CCGTGG C^C[GA][CT]GG EcoNI 139 CCTACAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 464 AGGACCT [GA]G^G[ACGT]CC[CT] FauI 302 GCGGG CCCGC(4/6) HphI 436 TCACC GGTGA(8/7) MaeII 329 ACGT A^CGT MboII 499 TCTTC GAAGA(8/7) MseI 26 TTAA T^TAA MwoI 269 GCCCAGGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PflMI 503 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 415 GACTC GAGTC(4/5) PmaCI 328 CACGTG CAC^GTG PmlI 328 CACGTG CAC^GTG PpuMI 464 AGGACCT [GA]G^G[AT]CC[CT] SfaNI 51 GATGC GCATC(5/9) SfcI 140 CTACAG C^T[GA][CT]AG TfiI 57 GATTC G^A[AT]TC Tru9I 26 TTAA T^TAA Van91I 503 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AvaII 448 GGACC G^G[AT]CC 465 GGACC BsmAI 121 GAGAC GTCTC(1/5) 160 GAGAC BsmFI 447 GGGAC GGGAC(10/14) 136 GTCCC BssKI 271 CCAGG ^CC[ACGT]GG 443 CCTGG BstNI 271 CCAGG CC^[AT]GG 443 CCTGG Cfr13I 448 GGACC G^G[ACGT]CC 465 GGACC EcoRII 271 CCAGG ^CC[AT]GG 443 CCTGG HinfI 57 GATTC G^A[ACGT]TC 415 GACTC MaeIII 326 GTCAC ^GT[ACGT]AC 435 GTCAC MvaI 271 CCAGG CC^[AT]GG 443 CCTGG NlaIV 259 GGTGCC GG[ACGT]^[ACGT]CC 447 GGGACC Sau96I 448 GGACC G^G[ACGT]CC 465 GGACC ScrFI 271 CCAGG CC^[ACGT]GG 443 CCTGG Tsp45I 326 GTCAC ^GT[GC]AC 435 GTCAC Tsp509I 107 AATT ^AATT 335 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AciI AluI BbvI BsaJI BsiYI BslI BstF5I Cac8I Fnu4HI FokI ItaI MnlI MspA1I PvuII TseI