################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AgeI AhdI Alw44I AlwI AlwNI AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BbrPI BciVI BclI BfrI BlnI BsaBI BseRI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrGI BsrI BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I ClaI DraI DraIII DrdI Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI MamI MfeI MluI MluNI MscI MseI MunI NdeI NheI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SalI SbfI ScaI SexAI SfiI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 472 GTCTAC GT^[AC][GT]AC AccIII 1059 TCCGGA T^CCGGA AflIII 296 ACGTGT A^C[GA][CT]GT ApaI 710 GGGCCC GGGCC^C AviII 395 TGCGCA TGC^GCA BbvCI 991 CCTCAGC CCTCAGC(-5/-2) BcgI 110 GCAACGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 879 AGATCT A^GATCT BsaAI 295 TACGTG [CT]AC^GT[GA] BsaI 1028 GAGACC GGTCTC(1/5) BseAI 1059 TCCGGA T^CCGGA BspEI 1059 TCCGGA T^CCGGA BspMI 895 GCAGGT ACCTGC(4/8) BsrDI 1022 GCAATG GCAATG(2/0) BssHII 302 GCGCGC G^CGCGC BstBI 922 TTCGAA TT^CGAA BstF5I 1043 GGATG GGATG(2/0) BstYI 879 AGATCT [GA]^GATC[CT] CpoI 1056 CGGTCCG CG^G[AT]CCG DpnII 880 GATC ^GATC EarI 919 CTCTTC CTCTTC(1/4) EcoNI 732 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FokI 1043 GGATG GGATG(9/13) FspI 395 TGCGCA TGC^GCA HinfI 555 GACTC G^A[ACGT]TC Ksp632I 919 CTCTTC CTCTTC(1/4) KspI 1017 CCGCGG CCGC^GG MboI 880 GATC ^GATC MflI 879 AGATCT [GA]^GATC[CT] MroI 1059 TCCGGA T^CCGGA MslI 174 CACGCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 971 CCATGG C^CATGG NdeII 880 GATC ^GATC NotI 950 GCGGCCGC GC^GGCCGC NspI 311 GCATGT [GA]CATG^[CT] NspV 922 TTCGAA TT^CGAA PleI 555 GACTC GAGTC(4/5) RsrII 1056 CGGTCCG CG^G[AT]CCG SacI 976 GAGCTC GAGCT^C SacII 1017 CCGCGG CCGC^GG SapI 918 GCTCTTC GCTCTTC(1/4) Sau3AI 880 GATC ^GATC SfaNI 588 GATGC GCATC(5/9) SfuI 922 TTCGAA TT^CGAA StuI 254 AGGCCT AGG^CCT TaqI 923 TCGA T^CGA TspRI 35 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 923 TCGA T^CGA XhoII 879 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### AcyI 384 GGCGCC G[GA]^CG[CT]C 628 GGCGCC AfaI 291 GTAC GT^AC 315 GTAC Aor51HI 353 AGCGCT AGC^GCT 750 AGCGCT AspHI 856 GTGCTC G[AT]GC[AT]^C 976 GAGCTC BanI 384 GGCGCC G^G[CT][GA]CC 628 GGCGCC BbeI 384 GGCGCC GGCGC^C 628 GGCGCC BbsI 48 GAAGAC GAAGAC(2/6) 865 GTCTTC BfaI 842 CTAG C^TAG 959 CTAG BpmI 550 CTGGAG CTGGAG(16/14) 875 CTGGAG BpuAI 48 GAAGAC GAAGAC(2/6) 865 GTCTTC BsaHI 384 GGCGCC G[GA]^CG[CT]C 628 GGCGCC BsaWI 762 ACCGGA [AT]^CCGG[AT] 1059 TCCGGA BsgI 149 CTGCAC GTGCAG(16/14) 184 CTGCAC BsiHKAI 856 GTGCTC G[AT]GC[AT]^C 976 GAGCTC DsaI 971 CCATGG C^C[GA][CT]GG 1017 CCGCGG EagI 464 CGGCCG C^GGCCG 951 CGGCCG EclXI 464 CGGCCG C^GGCCG 951 CGGCCG Eco47III 353 AGCGCT AGC^GCT 750 AGCGCT Eco52I 464 CGGCCG C^GGCCG 951 CGGCCG HgaI 609 GACGC GACGC(5/10) 845 GCGTC Hin1I 384 GGCGCC G[GA]^CG[CT]C 628 GGCGCC HincII 727 GTCAAC GT[CT]^[GA]AC 847 GTCAAC HindII 727 GTCAAC GT[CT]^[GA]AC 847 GTCAAC KasI 384 GGCGCC G^GCGCC 628 GGCGCC MaeI 842 CTAG C^TAG 959 CTAG MaeII 296 ACGT A^CGT 863 ACGT MaeIII 367 GTCAC ^GT[ACGT]AC 607 GTGAC NaeI 668 GCCGGC GCC^GGC 793 GCCGGC NarI 384 GGCGCC GG^CGCC 628 GGCGCC NgoMIV 668 GCCGGC G^CCGGC 793 GCCGGC NlaIII 312 CATG CATG^ 972 CATG PpuMI 897 AGGTCCC [GA]G^G[AT]CC[CT] 1067 GGGACCT PstI 349 CTGCAG CTGCA^G 736 CTGCAG PvuII 505 CAGCTG CAG^CTG 994 CAGCTG RsaI 291 GTAC GT^AC 315 GTAC SfoI 384 GGCGCC GGC^GCC 628 GGCGCC SmaI 890 CCCGGG CCC^GGG 1032 CCCGGG Tsp45I 367 GTCAC ^GT[GC]AC 607 GTGAC XmaCI 890 CCCGGG C^CCGGG 1032 CCCGGG XmaI 890 CCCGGG C^CCGGG 1032 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AccII AciI AluI AvaI AvaII BanII BbvI BcnI BglI BlpI BmyI Bpu1102I BsaJI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BssKI BstNI BstUI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI SfcI StyI TseI