(Circular) (Six-base) MAPSORT of: pFN21AE3032.seq Check: 993 from: 1 to: 5188 pFN21AE3032 5188 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2998 278 Size: 53 83 186 936 1462 2468 Fragments arranged by size: 2468 1462 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2531 1937 Size: 594 4594 Acc65I G'GTAC_C Cuts at: 2509 2509 Size: 5188 AclI AA'CG_TT Cuts at: 1563 3315 3688 1563 Size: 1752 373 3063 Fragments arranged by size: 3063 1752 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2383 2436 3251 4635 4903 1632 Size: 751 53 815 1384 268 1917 Fragments arranged by size: 1917 1384 815 751 268 53 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4991 AflIII A'CryG_T Cuts at: 1203 4087 5064 1203 Size: 2884 977 1327 Fragments arranged by size: 2884 1327 977 AgeI A'CCGG_T Cuts at: 4019 4019 Size: 5188 AhdI GACnn_n'nnGTC Cuts at: 3917 3917 Size: 5188 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5188 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2291 1184 Size: 1107 4081 AlwNI CAG_nnn'CTG Cuts at: 2931 4503 2931 Size: 1572 3616 ApaLI G'TGCA_C Cuts at: 3245 4401 3245 Size: 1156 4032 ApoI r'AATT_y Cuts at: 1493 2497 2723 2875 4984 1493 Size: 1004 226 152 2109 1697 Fragments arranged by size: 2109 1697 1004 226 152 AseI AT'TA_AT Cuts at: 160 1032 3742 160 Size: 872 2710 1606 Fragments arranged by size: 2710 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5188 AvaI C'yCGr_G Cuts at: 1870 1943 2513 1870 Size: 73 570 4545 Fragments arranged by size: 4545 570 73 BamHI G'GATC_C Cuts at: 2518 2885 2518 Size: 367 4821 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2258 2509 3964 618 Size: 325 217 627 471 251 1455 1842 Fragments arranged by size: 1842 1455 627 471 325 251 217 BanII G_rGCy'C Cuts at: 1391 2507 1391 Size: 1116 4072 BbeI G_GCGC'C Cuts at: 2262 2262 Size: 5188 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2447 928 Size: 971 548 3669 Fragments arranged by size: 3669 971 548 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3379 3413 1903 Size: 34 1442 34 3678 Fragments arranged by size: 3678 1442 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3080 4290 3080 Size: 1210 3978 BclI T'GATC_A Cuts at: 1548 1548 Size: 5188 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3799 136 Size: 107 122 71 631 605 2127 1525 Fragments arranged by size: 2127 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5183 5183 Size: 5188 BlpI GC'TnA_GC Cuts at: 2219 2605 2219 Size: 386 4802 Bme1580I G_kGCm'C Cuts at: 1790 3249 4405 1790 Size: 1459 1156 2573 Fragments arranged by size: 2573 1459 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5188 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2919 3877 442 Size: 416 53 83 399 24 1502 958 1753 Fragments arranged by size: 1753 1502 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5188 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2252 3848 1239 Size: 1013 1596 2579 Fragments arranged by size: 2579 1596 1013 Bpu10I CC'TnA_GC Cuts at: 2033 2033 Size: 5188 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3313 4178 4476 3313 Size: 865 298 4025 Fragments arranged by size: 4025 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2135 3851 882 Size: 617 636 1716 2219 Fragments arranged by size: 2219 1716 636 617 BsaAI yAC'GTr Cuts at: 493 1204 2245 2979 493 Size: 711 1041 734 2702 Fragments arranged by size: 2702 1041 734 711 BsaBI GATnn'nnATC Cuts at: 5182 5182 Size: 5188 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2259 2995 3377 4012 Size: 53 83 186 936 726 736 382 635 Cuts at: 4012 275 Size: 1451 Fragments arranged by size: 1451 936 736 726 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3621 4019 4293 4440 4851 3621 Size: 398 274 147 411 3958 Fragments arranged by size: 3958 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4963 Fragments arranged by size: 4963 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5188 BseYI C'CCAG_C Cuts at: 4391 4887 4391 Size: 496 4692 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 1163 Size: 573 345 4270 Fragments arranged by size: 4270 573 345 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2041 2638 3399 4427 1540 Size: 275 186 40 597 761 1028 2301 Fragments arranged by size: 2301 1028 761 597 275 186 40 BsiHKAI G_wGCw'C Cuts at: 2220 3249 3334 4405 2220 Size: 1029 85 1071 3003 Fragments arranged by size: 3003 1071 1029 85 BsmI GAATG_Cn' Cuts at: 2699 2792 2699 Size: 93 5095 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2220 2507 3249 3334 4405 1391 Size: 399 430 287 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 430 399 287 85 BspEI T'CCGG_A Cuts at: 4851 4851 Size: 5188 BspHI T'CATG_A Cuts at: 3077 4807 3077 Size: 1730 3458 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2543 844 Size: 1699 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3075 1394 Size: 429 1252 3507 Fragments arranged by size: 3507 1252 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3683 3857 65 Size: 1183 2435 174 1396 Fragments arranged by size: 2435 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3832 4019 1700 Size: 110 2022 187 2869 Fragments arranged by size: 2869 2022 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5188 BssHII G'CGCG_C Cuts at: 1925 2027 1925 Size: 102 5086 BssSI C'ACGA_G Cuts at: 3248 4260 3248 Size: 1012 4176 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5188 BstBI TT'CG_AA Cuts at: 3992 3992 Size: 5188 BstEII G'GTnAC_C Cuts at: 1184 2464 1184 Size: 1280 3908 BstXI CCAn_nnnn'nTGG Cuts at: 2269 2269 Size: 5188 BstYI r'GATC_y Cuts at: 1972 2364 2518 2885 3270 3287 4739 4825 4837 Size: 392 154 367 385 17 1452 86 12 Cuts at: 4837 5183 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 392 385 367 346 154 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2052 2310 513 Size: 552 987 258 3391 Fragments arranged by size: 3391 987 552 258 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2008 487 Size: 683 56 782 3667 Fragments arranged by size: 3667 782 683 56 BtsI GCAGTG_nn' Cuts at: 2704 3498 3518 2704 Size: 794 20 4374 Fragments arranged by size: 4374 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5188 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5153 DraI TTT'AAA Cuts at: 2492 2492 Size: 5188 DraIII CAC_nnn'GTG Cuts at: 2104 2104 Size: 5188 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4195 809 Size: 507 2879 1802 Fragments arranged by size: 2879 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2635 3524 5153 Size: 54 1425 50 275 18 805 889 1629 Cuts at: 5153 8 Size: 43 Fragments arranged by size: 1629 1425 889 805 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2635 1537 Size: 275 823 4090 Fragments arranged by size: 4090 823 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3118 1353 Size: 1765 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3775 4149 4295 387 Size: 1064 138 2186 374 146 1280 Fragments arranged by size: 2186 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 2383 2436 3251 3848 4635 4903 Size: 393 620 131 53 815 597 787 268 Cuts at: 4903 1239 Size: 1524 Fragments arranged by size: 1524 815 787 620 597 393 268 131 53 EcoNI CCTnn'n_nnAGG Cuts at: 2348 2348 Size: 5188 EcoRI G'AATT_C Cuts at: 2497 2875 2497 Size: 378 4810 FspI TGC'GCA Cuts at: 3694 3694 Size: 5188 HaeII r_GCGC'y Cuts at: 1678 2262 2304 4335 1678 Size: 584 42 2031 2531 Fragments arranged by size: 2531 2031 584 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2099 2131 3829 3861 3903 Size: 32 135 32 527 32 1698 32 42 Cuts at: 3903 3935 1373 Size: 32 2626 Fragments arranged by size: 2626 1698 527 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2532 2778 669 Size: 988 281 594 246 3079 Fragments arranged by size: 3079 988 594 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5188 HpaI GTT'AAC Cuts at: 2778 2778 Size: 5188 KasI G'GCGC_C Cuts at: 2258 2258 Size: 5188 KpnI G_GTAC'C Cuts at: 2513 2513 Size: 5188 MfeI C'AATT_G Cuts at: 2787 2787 Size: 5188 MluI A'CGCG_T Cuts at: 5064 5064 Size: 5188 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2505 4302 4486 1305 Size: 1200 1797 184 2007 Fragments arranged by size: 2007 1797 1200 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3366 Fragments arranged by size: 3366 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2051 2213 2267 3146 3505 3664 518 Size: 648 885 162 54 879 359 159 2042 Fragments arranged by size: 2042 885 879 648 359 162 159 54 MspA1I CmG'CkG Cuts at: 1606 2604 3281 4429 4674 1606 Size: 998 677 1148 245 2120 Fragments arranged by size: 2120 1148 998 677 245 NarI GG'CG_CC Cuts at: 2259 2259 Size: 5188 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4636 NdeI CA'TA_TG Cuts at: 387 387 Size: 5188 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5188 NotI GC'GGCC_GC Cuts at: 2635 2635 Size: 5188 NspI r_CATG'y Cuts at: 1132 1229 2546 4091 1132 Size: 97 1317 1545 2229 Fragments arranged by size: 2229 1545 1317 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5188 PciI A'CATG_T Cuts at: 4087 4087 Size: 5188 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5188 PmeI GTTT'AAAC Cuts at: 2492 2492 Size: 5188 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2060 2092 3257 3289 4770 4802 2060 Size: 32 1165 32 1481 32 2446 Fragments arranged by size: 2446 1481 1165 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5188 PsiI TTA'TAA Cuts at: 2758 2758 Size: 5188 PstI C_TGCA'G Cuts at: 830 2019 2540 830 Size: 1189 521 3478 Fragments arranged by size: 3478 1189 521 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5188 SalI G'TCGA_C Cuts at: 1936 2530 1936 Size: 594 4594 SbfI CC_TGCA'GG Cuts at: 2540 2540 Size: 5188 ScaI AGT'ACT Cuts at: 3436 5148 3436 Size: 1712 3476 SexAI A'CCwGG_T Cuts at: 2100 2100 Size: 5188 SfcI C'TryA_G Cuts at: 826 1045 2015 2368 2536 3671 4352 4543 826 Size: 219 970 353 168 1135 681 191 1471 Fragments arranged by size: 1471 1135 970 681 353 219 191 168 SfoI GGC'GCC Cuts at: 2260 2260 Size: 5188 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5188 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5188 SmaI CCC'GGG Cuts at: 1872 2515 1872 Size: 643 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3292 4193 4455 820 Size: 197 926 1349 901 262 1553 Fragments arranged by size: 1553 1349 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2979 493 Size: 2486 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5188 SphI G_CATG'C Cuts at: 1132 2546 1132 Size: 1414 3774 SspI AAT'ATT Cuts at: 5 52 3112 5 Size: 47 3060 2081 Fragments arranged by size: 3060 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5188 StyI C'CwwG_G Cuts at: 513 1065 2627 513 Size: 552 1562 3074 Fragments arranged by size: 3074 1562 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3215 3232 3385 3570 4015 325 Size: 1255 1635 17 153 185 445 1498 Fragments arranged by size: 1635 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3434 5146 96 Size: 274 80 33 51 1443 1457 1712 138 Fragments arranged by size: 1712 1457 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5148 5180 5148 Size: 32 5156 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5188 XbaI T'CTAG_A Cuts at: 2524 2524 Size: 5188 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5188 XmaI C'CCGG_G Cuts at: 1870 2513 1870 Size: 643 4545 XmnI GAAnn'nnTTC Cuts at: 3317 3317 Size: 5188 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2996 276 Size: 53 83 186 936 1462 2468 Fragments arranged by size: 2468 1462 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SrfI SwaI XcmI